Water use efficiency (WUE) reflects the balance between carbon assimilation and water consumption in terrestrial ecosystems. Considering the fragile conditions of global water resources, the evaluation of regional WUE variation characteristics and response mechanisms is critical for promoting sustainable ecological development and water resource utilization. Based on gross primary productivity (GPP) and evapotranspiration (ET) datasets, combined with vegetation and meteorological data, this study examined the spatiotemporal variations, annual variation contribution rate, and driving mechanism of WUE in Inner Mongolia from 2001 to 2020. The main results are as follows: (1) The interannual and spatial variations of GPP, ET, and WUE all exhibited increasing trends, with WUE increasing in approximately 70% of the region and significantly increasing in 22.35% of pixels exhibiting a significant increase. Areas with the most significant increases were located in the Horqin sandy land and Mu Us sandy land. (2) The highest WUE values were observed in the summer season, followed by autumn and winter, and the lowest in spring. (3) Among all vegetation types, the typical steppe ecosystems contributed most to the interannual variability (IAV) of GPP, ET, and WUE, with values of 169.89%, 141.09%, and 193.42%, respectively. While the coniferous forest contributed least or negatively to GPP, ET, and WUE IAV, with values of −36.28%, 28.20%, and −32.86%, respectively. (4) The primary driver of WUE variation was found to be GPP, which contributed 59.36%, mainly in the central and western regions. The remaining 40.64% was attributable to ET, concentrated in the northeast region. (5) Human activities significantly affected WUE, with a contribution (about 53.52%) larger than that of climate change (nearly 46.48%). Increased precipitation improves vegetation WUE and is the most important climate factor influencing WUE variations. These findings will aid the formulation of vegetation protection and water resource management strategies in water-stressed areas.
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