Plant Water Use Efficiency Research Articles

Optimizing plastic mulching improves the growth and increases grain yield and water use efficiency of spring maize in dryland of the Loess Plateau in China

Transparent plastic film mulching (PFM) with flat cropping cultivation has been widely applied to grow maize (Zea mays L.) in dryland agricultural production areas of the Loess Plateau in China. However, with global warming-induced gradual increase in soil temperature, film-mulched spring maize experiences premature senescence and yield loss. In this study, a new form of mulching strategy involving dual mulching of transparent plastic film with whole maize stalks in a flat plot was established to improve growth and increase water use efficiency (WUE), precipitation use efficiency (PUE), and spring maize yield. The field experiment was conducted in 20 182 019, and 2020 on a conventional flat plot without mulching (CK), a flat plot with single transparent PFM, and a flat plot of transparent film mulching with whole maize stalks (PFM+ST) in the dryland of the Loess Plateau in China, to quantify the effects of different treatments on soil hydrothermal conditions, maize phenology, plant growth dynamics, WUE, PUE, and maize yield. Results demonstrated that soil water storage (SWS) at 0–100 cm soil layer in PFM and PFM+ST were significantly superior to those in CK, but SWS did not significantly differ between PFM and PFM+ST. Soil temperature under PFM+ST at 0–25 cm soil layer decreased by 1.2 ℃ compared with that under PFM during growth, especially in early growing seasons (20–60 days after sowing [DAS]). PFM+ST extended vegetative and reproductive growth periods by 1–2 and 6–7 days compared with those in PFM, respectively. In later growth period (80–120 DAS), PFM+ST increased the root dry matter weight, SPAD, and LAI and significantly increased the aboveground dry matter accumulation. PFM+ST significantly increased the 100-kernel dry weight, grain yield, WUE, and PUE. PFM+ST had 3-year average grain yields of 10.82% and 36.48% greater than PFM and CK, respectively. The corresponding WUE and in 0–200 cm soil layer improved by 12.55% and 39.84%, and PUE improved by 10.82% and 36.48%, respectively. With global warming, applying PFM+ST cultivation practice effectively extended growth period and increased mulching spring maize yield and WUE in the study site.

Combining Planting Patterns with Mulching Bolsters the Soil Water Content, Growth, Yield, and Water Use Efficiency of Spring Wheat under Limited Water Supply in Arid Regions

Innovations in water-saving cultivation strategies are urgently needed to achieve high yield and elevated water use efficiency (WUE) simultaneously in arid regions with limited water resources. Here, we conducted a two-year field study to compare the impacts of eight combinations of planting patterns (PPs) and mulching on the soil water content (SWC) in the top 60 cm soil layer, the growth, the yield, and the WUE of wheat under two irrigation rates (1.00 and 0.50 ET). These combinations included three conventional flat planting (CF) patterns, including CF without mulch (CFNM), with plastic film (CFPM), and with wheat straw mulch (CFSM); three raised-bed planting (RB) patterns, including RB without mulch (RBNM), with plastic film (RBPM), and wheat straw (RBSM) mulch; and two ridge–furrow planting (RF) patterns, including RF without mulch (RFNM) and with plastic film mulch (RFPM). The results showed that the tested treatments affected the SWC at different depths under both irrigation rates. Compared with the two non-mulched treatments under 0.50 ET, the SWC of the three PPs with plastic film and the two PPs with wheat straw mulching were significantly higher before irrigation by 14.4–22.0% and 6.9–17.2% at 0–20 cm soil depth, 16.4–29.0% and 6.6–14.9% at 20–40 cm soil depth, and 3.3–34.8% and 3.4–14.5% at 40–60 cm soil depth, respectively. All measured wheat parameters, except harvest index, were significantly affected by the interaction between irrigation rate and PPs. The highest values for plant dry weight (PDW), yield components, grain yield (GY), and WUE under 1.00 ET were obtained in the two PPs with wheat straw mulch, while the three PPs with plastic film showed the highest values of these parameters under 0.50 ET. The yield response factor (Ky) based on PDW was acceptable for all PPs mulched with plastic film and wheat straw as well as for RFNM, while Ky based on GY was acceptable only for the PPs mulched with plastic film and for RFNM, as the Ky values of these PPs were less than 1 under 0.50 ET. The SWC at different depths exhibited quadratic and nonsignificant relationships with all parameters under 1.00 ET, while these relationships were linear and strong under 0.50 ET, with a few exceptions. Overall, we conclude that combining any PPs with plastic film mulching could be used as a feasible and effective strategy for obtaining high wheat yield and WUE in the irrigated and arid agroecosystem.

A deep learning-integrated phenotyping pipeline for vascular bundle phenotypes and its application in evaluating sap flow in the maize stem

Plant vascular bundles are responsible for water and material transportation, and their quantitative and functional evaluation is desirable in plant research. At the single-plant level, the number, size, and distribution of vascular bundles vary widely, posing a challenge to automatically and accurately identifying and quantifying them. In this study, a deep learning-integrated phenotyping pipeline was developed to robustly and accurately detect vascular bundles in Computed Tomography (CT) images of stem internodes. Two semantic indicators were used to evaluate and identify a suitable feature extraction network for semantic segmentation models. The epidermis thickness of maize stem was evaluated for the first time and adjacent vascular bundles were improved using an adaptive watershed-based approach. The counting accuracy (R2) of vascular bundles was 0.997 for all types of stem internodes, and the measured accuracy of size traits was over 0.98. Combining sap flow experiments, multiscale traits of vascular bundles were evaluated at the single-plant level, which provided an insight into the water use efficiency of the maize plant.

Guard-cell-targeted overexpression of Arabidopsis Hexokinase 1 can improve water use efficiency in field-grown tobacco plants.

Water deficit currently acts as one of the largest limiting factors for agricultural productivity worldwide. Additionally, limitation by water scarcity is projected to continue in the future with the further onset of effects of global climate change. As a result, it is critical to develop or breed for crops that have increased water use efficiency and that are more capable of coping with water scarce conditions. However, increased intrinsic water use efficiency (iWUE) typically brings a trade-off with CO2 assimilation as all gas exchange is mediated by stomata, through which CO2 enters the leaf while water vapor exits. Previously, promising results were shown using guard-cell-targeted overexpression of hexokinase to increase iWUE without incurring a penalty in photosynthetic rates or biomass production. Here, two homozygous transgenic tobacco (Nicotiana tabacum) lines expressing Arabidopsis Hexokinase 1 (AtHXK1) constitutively (35SHXK2 and 35SHXK5) and a line that had guard-cell-targeted overexpression of AtHXK1 (GCHXK2) were evaluated relative to wild type for traits related to photosynthesis and yield. In this study, iWUE was significantly higher in GCHXK2 compared with wild type without negatively impacting CO2 assimilation, although results were dependent upon leaf age and proximity of precipitation event to gas exchange measurement.

Variations in Plant Water Use Efficiency Response to Manipulated Precipitation in a Temperate Grassland

Water use efficiency (WUE) plays important role in understanding the interaction between carbon and water cycles in the plant-soil-atmosphere system. However, little is known regarding the impact of altered precipitation on plant WUE in arid and semi-arid regions. The study examined the effects of altered precipitation [i.e., ambient precipitation (100% of natural precipitation), decreased precipitation (DP, −50%) and increased precipitation (IP, +50%)] on the WUE of grass species (Stipa grandis and Stipa bungeana) and forb species (Artemisia gmelinii) in a temperate grassland. The results found that WUE was significantly affected by growth stages, precipitation and plant species. DP increased the WUE of S. grandis and S. bungeana generally, but IP decreased WUE especially in A. gmelinii. And the grasses had the higher WUE than forbs. For different growth stages, the WUE in the initial growth stage was lower than that in the middle and late growth stages. Soil temperature, available nutrients (i.e., NO3–, NH4+, and AP) and microorganisms under the altered precipitations were the main factors affecting plant WUE. These findings highlighted that the grasses have higher WUE than forbs, which can be given priority to vegetation restoration in arid and semi-arid areas.

Transcriptome and Physiological Analyses of a Navel Orange Mutant with Improved Drought Tolerance and Water Use Efficiency Caused by Increases of Cuticular Wax Accumulation and ROS Scavenging Capacity.

Drought is one of the main abiotic stresses limiting the quality and yield of citrus. Cuticular waxes play an important role in regulating plant drought tolerance and water use efficiency (WUE). However, the contribution of cuticular waxes to drought tolerance, WUE and the underlying molecular mechanism is still largely unknown in citrus. ‘Longhuihong’ (MT) is a bud mutant of ‘Newhall’ navel orange with curly and bright leaves. In this study, significant increases in the amounts of total waxes and aliphatic wax compounds, including n-alkanes, n-primary alcohols and n-aldehydes, were overserved in MT leaves, which led to the decrease in cuticular permeability and finally resulted in the improvements in drought tolerance and WUE. Compared to WT leaves, MT leaves possessed much lower contents of malondialdehyde (MDA) and hydrogen peroxide (H2O2), significantly higher levels of proline and soluble sugar, and enhanced superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activities under drought stress, which might reduce reactive oxygen species (ROS) damage, improve osmotic regulation and cell membrane stability, and finally, enhance MT tolerance to drought stress. Transcriptome sequencing results showed that seven structural genes were involved in wax biosynthesis and export, MAPK cascade, and ROS scavenging, and seven genes encoding transcription factors might play an important role in promoting cuticular wax accumulation, improving drought tolerance and WUE in MT plants. Our results not only confirmed the important role of cuticular waxes in regulating citrus drought resistance and WUE but also provided various candidate genes for improving citrus drought tolerance and WUE.

The New Soil Conditioner DewEco Could Improve Sandy Soil’s Properties for Efficient Maize Growth

Sandy soil, one of the most abundant soil types in the world, often has lower crop productivity because of poor water and fertilizer retention capacity. The objective of this study was to investigate the effects of the new soil conditioner DewEco (fermented organic material consisted mainly of salt of L-lysine and citric acid) on sandy soil quality and plant growth. Serial dosages of DewEco and nitrogen (N) fertilizer were mixed into sandy soils and planted maize in a greenhouse. DewEco application increased large soil particle composition and decreased small soil particle composition. Soil porosity and the liquid phase increased as the DewEco dosage increased. DewEco also decreased soil pH and increased soil electrical conductivity, soil organic matter content, total nitrogen and available potassium. DewEco significantly enhanced the soil water-holding capacity and soil effective water content although it also increased the wilting coefficient. Finally, DewEco markedly promoted maize growth while improving water use efficiency (WUE) and nitrogen use efficiency (NUE). In addition, there was an interaction effect between DewEco and nitrogen fertilizer, such that the combined effects of DewEco and N exceeded the sum of their respective effects promoting plant growth. Thus, DewEco application can significantly enhance soil water content and nutrient levels by alleviating sandy soil’s physical and chemical properties, thereby promoting plant growth, WUE and NUE. This study indicates that DewEco is a useful eco-friendly sandy soil conditioner for arid and semi-arid regions.

Effects of fulvic acid on photosynthetic characteristics, yield and quality of cucumber under drought stress

Fulvic acid (FA) participates in the regulation of drought stress tolerance in plants, but the underlying mechanisms remain unclear. We carried out an experiment with cucumber cultivar 'Jinyou 35' as the test material and the polyethylene glycol (PEG-6000) being used to simulate drought stress. The concentration effect of FA on drought alleviation of cucumber as well as the effect of FA on photosynthetic enzymes activities, chloroplast ultrastructure, fluorescence parameters, water use efficiency, yield and quality of cucumber plants were studied through spraying FA with different concentrations (0, 100, 300, 500, 700 and 900 mg·L-1). The results showed that FA with different concentrations significantly promoted the relative water content and leaf area and decreased drought injure index as well as the electrolyte leakage and malondialdehyde (MDA) content, compared with the control (0 mg·L-1) under drought stress. The mitigative effect of FA increased first and then decreased with the increases of FA concentration, with 700 mg·L-1 FA showing the best effect. FA significantly enhanced the chlorophyll content, Rubiasco and Rubisco activase (RCA) activities and gene expression, net photosynthesis (Pn), maximal photochemical efficiency of PSⅡin darkness, actual photochemical efficiency, absorbed light energy per unit area, captured light energy per unit area, quantum yield of electron transport and PSⅠ activity, decreased the increase of K point and maintained chloroplast ultrastructure. The experiment in solar-greenhouse showed that FA obviously increased water use efficiency, promoted dry matter accumulation as well as the contents of Vc, soluble sugar, soluble protein and free amino acid, and decreased tannin content. Our results suggested that FA could improve the yield and quality of cucumber in solar greenhouse under drought stress.

Effects of Supplementary Irrigation and Nitrogen Fertilization on Characteristics of Sun Cured Tobacco in Semi-Arid Conditions

ABSTRACT Nitrogen and water should be applied at the right time and amount to obtain optimum crop yield in arid and semi-arid regions. The study was carried out in 2017–2018 to investigate the effects of different supplementary irrigation levels applied after harvests, and different nitrogen sources on water use efficiency (WUE), yield and quality characteristics of sun-cured tobacco. The study included five different nitrogen sources (N1: zero-nitrogen, N2: ammonium nitrate, N3: ammonium sulfate, N4: calcium ammonium nitrate, and N5: urea) and four different irrigation treatments (I0:No irrigation; I33:66% deficit, I66:33% deficit and I100:100% full irrigation). The effects of nitrogen sources and supplementary irrigation treatments on plant height, leaf number, leaf width, leaf height, yield, quality grade, sugars, nicotine, phenolics, WUE and irrigation water use efficiency (IWUE) were statistically significant. Seasonal crop evapotranspiration values ranged between 117.7 and 235.3 mm. The highest WUE 1.65 kg m−3 and IWUE: 10.48 kg m−3 were recorded in I33 treatment. Plant characteristics were different between nitrogen sources; however, the results did not indicate a prominent nitrogen form. The results for supplementary irrigation revealed that the I100 treatment in semi-arid conditions increased the sun-cured tobacco yield by 22% with acceptable quality improvements. The gross income increased 1705 $ ha−1 with the increase in tobacco yield and sale price per hectare.

Effects of Plant Growth Promoting Rhizobacteria (PGPR) Strain Bacillus licheniformis with Biochar Amendment on Potato Growth and Water Use Efficiency under Reduced Irrigation Regime

The objective of this study was to explore the effects of plant growth-promoting rhizobacteria (PGPR), strain Bacillus licheniformis, with softwood biochar amendment on potato growth and water use efficiency (WUE) under a deficit irrigation (DI) regime. A pot experiment was conducted in a greenhouse. The results showed that PGPR improved leaf gas exchange rates, including photosynthesis rate, stomatal conductance and transpiration rate at early seedling stage, while tended to depress these parameters gradually until final harvest. The effects of biochar on plant leaf physiology, plant growth and WUE were not evident. Plants were more affected by DI than PGPR inoculation and biochar amendment. DI significantly decreased leaf gas exchange rates after exposure to water treatment for around three weeks, and the negative effect was eliminated at final harvest. At final harvest, DI significantly decreased leaf area, specific leaf area, dry mass of leaf and stem, total dry mass, dry mass increment and plant water use. The synergistical effect of PGPR strain Bacillus licheniformis and DI on plant growth and WUE were not observed in our study. WUE was solely improved by DI, indicating that, compared to PGPR inoculation, DI was a more effective measure to enhance plant WUE.

Effects of increasing CO2 concentration and water deficit on photosynthetic performance and water use efficiency of typical green manure plants

Exploring the impacts of CO2 and soil water availability on the photosynthetic performance and water use efficiency of three green manure plants could provide theoretical basis for the adaptive management of grassland ecosystems under future climate change. An experiment was conducted in an artificial climate chamber with precisely controled CO2 concentrations of 400 (natural atmospheric) and 800 μmol·mol-1 (doubled), and four water treatments, 80% field water holding capacity (FC) (full irrigation control group), 55%-60% FC (mild water deficit), 35%-40% FC (moderate water deficit), <35% FC (severe water deficit) to investigate the impacts of increasing CO2 concentration and water deficit on chlorophyll content, gas exchange variables, and water use efficiency (WUE) of oilseed rape (Brassica napus), white clover (Trifolium repens), and alfalfa (Medicago sativa). The results showed that under the same CO2 concentration, when soil moisture was less than 40% FC, the chlorophyll content and gas exchange parameters of three plants were significantly decreased. The treatment of 55%-60% FC did not alter the total chlorophyll content of three species, but reduced the photosynthetic rate (Pn) and transpiration rate (Tr) of white clover and alfalfa by 6%-25% and did not affect their WUE. Compared with atmospheric CO2 concentration, the doubled CO2 concentration significantly decreased the Pn of oilseed rape by 21.5% under the full irrigation treatment, increased the Pn of three species under mild water deficit, increased the Pn of oilseed rape and alfalfa under moderate water deficit, but only improved the Pn of alfalfa under severe water deficit. The doubled CO2 concentration significantly increased WUE of white clover and alfalfa under all water deficit conditions, but only increased WUE of oilseed rape under mild water deficit. Increasing CO2 concentration and water deficit significantly interacted to affect Pn of three species and the WUE of oilseed rape. In summary, the three species differed in their responses to doubled atmospheric CO2 concentration and different levels of water deficit. Our results suggested that elevated CO2 concentration could improve the adverse effects of mild water deficit on photosynthetic performance and WUE of three species, but only improve the photosynthetic performance of alfalfa under severe water deficit.

Elevated CO2 effect on the response of stomatal control and water use efficiency in amaranth and maize plants to progressive drought stress

Rising CO2 concentration ([CO2]) in the atmosphere may modulate the response of crop plants to drought stress. This study aimed to investigate the response of leaf gas exchange and plant growth of two C4 species representing both dicot (amaranth) and monocot (maize) to progressive drought under two different [CO2] (ambient (a[CO2], 400 ppm) and elevated (e[CO2], 800 ppm)). The soil water status in the pots was expressed as the fraction of transpirable soil water (FTSW). The results showed that as compared to a[CO2], e[CO2] significantly increased net photosynthetic rate (An) at non-stress condition (An max) for both species, while the increase was more pronounced in maize than in amaranth. Stomatal conductance (gs) at non-stress condition was significantly lower at e[CO2] in both species. The FTSW threshold, at which An starts to decrease, was higher in maize grown at e[CO2] than at a[CO2], whereas it was not affected in amaranth. In both species, gs decreased at higher FTSW threshold when grown at e[CO2] than at a[CO2]. e[CO2] decreased stomatal density (SD) in amaranth but increased it in maize; drought increased SD in amaranth but not in maize. Intrinsic water use efficiency (WUEi) was significantly enhanced by e[CO2] and drought stress at FTSW ranged from 0.0 to 0.6, particularly in maize. e[CO2] increased leaf area of well-watered plants in maize and decreased specific leaf area in amaranth. In amaranth, water consumption of well-watered plants was increased and plant WUE was decreased at e[CO2]. The varied responses of leaf gas exchange and WUE to soil water deficits and e[CO2] among the two C4 species imply their different mechanisms in stomatal control over carbon gain versus water loss in dicot and monocot plants, which is essentially important for selecting crop species and developing strategies to optimize crop WUE in a future drier and CO2-enriched climate. The more physiological and biochemical response from soil, stomatal to plant scale related to various environments would be considered in further investigation.

Physiological response, phytohormone signaling, biomass production and water use efficiency of the CAM plant Ananas comosus under different water and nitrogen regimes

The objective of this study was to investigate and unravel the mechanisms for the impact of soil water regimes and N application rates on growth, physiological responses, phytohormone signaling, water use efficiency (WUE) and nitrogen recovery efficiency (NRE) of crassulacean acid metabolism (CAM) plant pineapple. The experimental treatments included three soil water regimes (90%, 70% and 50% of soil water holding capacity, representing well watered, mildly and moderately water stressed conditions) and N application rates (109, 218 and 473 mg kg-1 soil). Results showed that the well watered and mildly water stressed treatments increased the shoot dry biomass by 70.7–110.9% and the plant water use by 25.7–30.4%, consequently, the plant WUE was significantly improved compared to the moderately water stressed treatment. The specific leaf N content was significantly and positively correlated with plant carbon (C) accumulation that was increased by 66.9–89.9%, implying that the enhanced specific leaf N content in the well watered and mildly water stressed treatments could have facilitated the carbon fixation, thus increased the shoot biomass accumulation. Moreover, the well watered and mildly water stress treatments significantly increased leaf δ18O, indicating the significantly higher transpiration in line with the markedly increased plant water use due to both the large leaf water concentration and the characteristic diel pattern of stomatal conductance associated with CAM. The enhanced leaf δ13C and plant WUE in the well watered and mildly water stress treatments were ascribed to the enhanced specific leaf N content and the improved leaf relative water content. The moderately water stressed treatment decreased leaf and root water potential while significantly intensified root endogenous ABA due to water deficit. The [ZR], [IAA] and [GA3] in the leaves and roots interacted complicatedly with water and N rates. The well watered and mildly water stressed treatments enhanced ability of the roots to absorb water and nutrients from the soil, resulting in the significantly higher N and 15N accumulation. Conclusively, in the production of Ananas comosus maintaining high soil water supply is critical to achieve improved growth, water and fertilizer-N use efficiencies.

The Differential Response of Intracellular Water Metabolism Derived from Intrinsic Electrophysiological Information in Morus alba L. and Broussonetia papyrifera (L.) Vent. Subjected to Water Shortage

Plant electrical signals can quickly respond to the shifting environment. Almost all life activities of plants are dependent on water. The measurement of plant electrophysiological indices provides a more convenient method for studying the intracellular water utilization. In this study, Morus alba L. (Morus alba or M. alba) and Broussonetia papyrifera (L.) Vent. (Broussonetia papyrifera or B. papyrifera) were experimental materials, and the parameters were measured in two habitats (waterfront, well-water and arid slopes, deficient-water). The physiological and electrophysiological responses of leaves to different habitats were analyzed. The theoretically intrinsic relationships between the clamping force and leaf impedance (Z), capacitive reactance (Xc), resistance (R), and inductive reactance (Xl) were revealed as 3-parameter exponential decay and linear models based on bioenergetics, respectively. Leaf intrinsic electrophysiological parameters were successfully obtained by using the above-mentioned relationships and were used to manifest metabolic activity in plants. The intracellular water-holding capacity (IWHC), water use efficiency (IWUE), water-holding time (IWHT), and water transfer rate (WTR) of plant leaves were defined based on the intrinsic electrophysiological parameters and were used to reflect the intracellular water metabolism. The correlation between the physiological and electrophysiological parameters of the two plant species in the two habitats was also analyzed. The results showed that Morus alba continuously adapted to the shifting environment, the intracellular water metabolism was insensitive to soil water shortage and was independent from the external physiological state. The intracellular water metabolism in Broussonetia papyrifera was very sensitive to soil water shortage, and both intracellular water metabolism and immediate physiological parameters could characterize the response of Broussonetia papyrifera growth and development to soil water.

Effect of Colored Shading Nets on the Growth and Water Use Efficiency of Sweet Pepper Grown under Semi-arid Conditions

Colored shading nets have been increasingly studied in semi-arid crop production systems, primarily because of their ability to reduce solar radiation with the attendant reductions in air, plant, and soil temperatures. However, there is a paucity of research concerning the impact of colored shading nets on various crops grown under semi-arid environments, particularly the sweet pepper (Capsicum annum) production system. This study aimed to investigate the effects of three colored shading net treatments (i.e., white, green, and black shading nets with 50% shading intensity and control with unshaded conditions) on the growth and instantaneous water use efficiency (WUE) of sweet pepper. The results showed that all colored shading nets exhibited significantly lower daytime air temperatures and light intensity (22 to 28 °C and 9992 lx, respectively) compared with the control (32 to 37 °C and 24,973 lx, respectively). There were significant differences in sweet pepper growth performance among treatments, including plant height, shoot dry weight, leaf area, leaf chlorophyll content, and vitamin C in ripened fruit. The enhanced photosynthetic rates were observed in sweet pepper plants under the colored shading nets compared with control plants. WUE increased among the colored shading net treatments in the following order: control ≤ white < black < green. Overall, the application of green and black shading nets to sweet pepper production systems under semi-arid environments significantly enhanced plant growth responses and WUE.

Methods of application of salicylic acid as attenuator of salt stress in cherry tomato.

Salt stress caused by excess salts present in irrigation water, is one of the biggest barriers in agricultural production, especially in semi-arid regions. Thus, the use of substances, such as salicylic acid, that minimize the deleterious effects of salinity on plants can be an alternative to ensure satisfactory production. In this context, the objective of this study was to evaluate the effects of different methods of application of salicylic acid on the growth, production and water use efficiency of cherry tomato plants under salt stress. The study was conducted in a greenhouse, using an Entisol soil with a sandy loam texture. The treatments were distributed in a completely randomized design, in a 2×4 factorial arrangement, corresponding to two levels of electrical conductivity of irrigation water - ECw (0.6 and 2.6 dS m-1) and four methods of application of salicylic acid (Control - without application of SA; via spraying; via irrigation and via spraying and irrigation), with five replicates and one plant per plot. The salicylic acid concentration used in the different methods was 1.0 mM. Application of salicylic acid via foliar spraying increased the growth, production and water use efficiency of cherry tomato plants. The salt stress induced by the electrical conductivity of 2.6 dS m-1 was attenuated by the foliar application of salicylic acid. The use of water of 2.6 dS m-1 associated with the application of salicylic acid via irrigation water further intensified the adverse effects of salinity on cherry tomato plants.

Water and fertilizer use efficiency of lettuce plants cultivated in soilless conditions under different irrigation systems

Water and fertilizer use efficiency of lettuce plants cultivated in soilless conditions under different irrigation systems

Combined effects of irrigation level and fertilization practice on yield, economic benefit and water-nitrogen use efficiency of drip-irrigated greenhouse tomato

The over-application of irrigation and chemical fertilization in the greenhouse vegetable production system (GVPS) in China can jeopardize fruit quality, compromise economic profit and waste resources. Seeking optimal water and fertilizer supply mode for GVPS is urgently required to improve resource use efficiency and economic benefit. A greenhouse experiment was conducted during four consecutive tomato growing seasons to investigate the combined effects of various irrigation levels and fertilization practices on yield, fruit quality, economic benefit, water use efficiency (WUE) and nitrogen use efficiency (NUE) of drip-fertigated tomato, and to obtain the optimal water and fertilizer supply practice by the principal component analysis (PCA). On the basis of full irrigation (W1: 100%ETc, ETc was the crop evapotranspiration), two deficit irrigation levels (W2: 75%ETc, W3: 50%ETc) were set. Except for the conventional fertilization practice used by local farmers (CC: chicken manures for basal application and chemical fertilizer for topdressing), four other fertilization practices were considered, including soluble organic fertilizers for topdressing (SO), soluble chemical fertilizers for topdressing (SC), both soluble organic and chemical fertilizers for topdressing (SOSC), and a control with no fertilizer application under full irrigation (CK). The results showed that SOSC obtained relatively high plant nitrogen uptake, net profit and WUE, and significantly improved tomato yield. Organic fertilizers addition reduced nitrate content in tomato fruits. Deficit irrigation promoted CC to obtain higher WUE than SC. According to the result of PCA, the comprehensive ranking of SOSC was first among the fertilization practices, regardless of irrigation levels and growing seasons. W1SOSC and W2SOSC were highly recommended for greenhouse tomato production in spring and autumn seasons, respectively.

Effects of soil drought and vapour pressure deficit (VPD) on water use efficiency of tomato plants with contrasting endogenous ABA levels

To investigate the interaction and elusive underlying mechanism of soil drought and vapour pressure deficit (VPD) on plant growth and water use efficiency (WUE), tomato plants (Solanum lycopersicum) (wild type and its ABA-deficient mutant) were grown under two different VPD environments. The parameters like stomatal conductance (gs), plant water relations, WUE, abscisic acid concentration (ABA) in leaf and xylem sap, carbon and oxygen isotope composition in leaf (δ13Cleaf and δ18Oleaf) were measured to estimate the physiological basis of VPD and drought in these genotypes of tomato. The results showed that, in relation to relatively higher VPD (ca. 1.15 kPa), lower VPD (ca. 0.55 kPa) significantly improved plant water status including leaf relative water content, leaf water potential and root water potential. Lower VPD chamber increased dry mass increment but decreased daily transpiration and plant water use, hereby improving WUE. Under drought conditions, decrease in plant water status and gs occurred in the wild type plants, along with higher concentration of ABA in leaf and xylem sap. However, there was no significant difference in plant WUE between the soil-droughted plants and the well-watered controls. And the interaction of soil drought and VPD on WUE was not significant. In contrast, plant WUE was superior in the wild type plants, especially under lower VPD environment. These findings help to understand the mechanism of stomata response to drought under varied environments of VPD. It could provide useful information for tomato cultivation under climate-controlled environments.

Drought priming alleviated salinity stress and improved water use efficiency of wheat plants

Drought priming alleviated salinity stress and improved water use efficiency of wheat plants