Nitrogen pollution was the main factor leading to reservoir eutrophication. The removal of low-concentration nitrate pollutants in water source reservoirs is an international problem to be solved. Insufficient organic electron donors in natural reservoir are a crucial limiting factor for in situ denitrification. Microbial mixotrophic aerobic denitrification using iron as the assisted electron donor is a potential pathway to treat micropolluted water bodies. The iron/activated carbon (IAC) was used to be an assisted electron provider to advance the mixotrophic aerobic denitrification performance of denitrifying bacterial community in micro-polluted reservoir water. Upon adding IAC into the reactors, the total nitrogen (TN) reduction capacity improved >41.10 %. A slight increase in organic matter removal efficiency observed in the IAC reactors compared to the control reactors. High-throughput sequencing revealed that IAC altered the richness, and co-occurrence of denitrifying bacterial community, further advancing nitrate transformation and removal. The relative abundant of Thauera, Dechloromonas, Cupriavidus, Alicycliphilus, and Azoarcus increased and presented predominant in the IAC reactors. Meanwhile, network analysis, niche width model, neutral community model, and species abundance distribution supported this finding. The Jinpen reservoir (JP; 34°32'38" N, 107°53'53" E, located in Xi’an, North China) and Xikeng reservoir (XK; 34°32'38" N, 107°53'53" E, located in Shenzhen, South China) raw water treatment experiments demonstrated excellent TN (> 60 %) and organic matter removal efficiencies (> 44 %).