Drylands are characterised by spatially discontinuous vegetation coverage. Consequently, during most rainfalls, runoff is generated in open areas and redistributed to vegetation. This transfer of water and nutrients from source to sink areas has been identified as a key ecohydrological process modulating drylands functioning. However, there are only a few experimental studies assessing the role of runoff redistribution on vegetation, and none of them evaluate its effect on determining the response of vegetation patches to aridification. In this study, we conducted a 3-year runoff exclusion experiment on 8 study sites consisting of Macrochloa tenacissima steppes distributed along two aridity gradients with contrasting lithologies in SE Spain. The aim was to assess the effect of runoff redistribution on vegetation and underlying soil communities as aridity increases, and to determine the influence of lithology on this response. For that, we compared a set of plants (photosynthetic activity, leaf chlorophyll content and green biomass production) and soil (microbial activity and composition) traits measured during different field campaigns on plants receiving run-on with the same variables measured on plants where run-on was excluded. Our findings demonstrate that run-on increases plant green biomass during wet periods with control plants showing a gain of the green biomass fraction higher than run-on excluded plants (difference between control and excluded plants is about ∼10 % of the total AGB in both gradients). By altering green biomass accumulation, run-on exclusion also shapes soil microbial communities (∼20 % of the aridity dependent ASVs are affected by run-on exclusion) and ameliorates the negative effects of drought on the activity and biomass of soil microorganisms (the decrease of SIR values between time 1 and 2 is 17 % lower in control than on run-on excluded plants), but it does not affect photosynthetic rate and chlorophyll concentration. This is due to the poikilohydric behaviour of M. tenacissima and the contrasting timing in the response of the measured variables to water pulses. Vegetation photosynthetic rates respond to short-term water availability, while green biomass and its indirect effect on the soil microbial communities results from a delayed response to the different rainfalls during the growing season. The effects of run-on were lower than expected and did not vary along aridity gradients, probably due to the unusual drought conditions and the atypical rainfalls during the studied period. During the last years, only a few large rainfalls were registered, causing soil moisture saturation under both excluded and control plants. Under these conditions, resources provided by runoff to control plants is diminished, thus lessening their competitive position compared to excluded plots. Long-term monitoring is necessary to evaluate the response of vegetation and the underlying soil to resource supply through runoff water with increasing aridity.
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