Although Unmanned Aerial Vehicles (UAVs) are developed for military missions originally, they have been used widely in civil activities for several decades globally. In agricultural, UAVs have been developed as an efficient sprayer for pesticide application since 1987. UAV-based sprayers are popular for the prevention and control of pests and diseases in field crop in many countries recently. Some of the UAV-based sprayers are developed to be equipped with fruit tree mode aiming at solving droplet penetration in the inside and bottom part of the fruit tree canopy. In this study, a newly released UAV-based sprayer (i.e., T30) equipped fruit tree mode is chosen as spraying platform to optimize the spraying parameters for practical application. The flight velocity and application rate are the variables, while droplet coverage, density, size, and penetration are the observed metrics. Three treatments with different flight velocities (2 m s-1 or 3 m s-1) or application rates (60 L ha-1 or 75 L ha-1) are arranged to collect the droplets for assessment. Water Sensitive Papers (WSPs) are placed in the outside, bottom, and inside layers of the canopy to collect droplets. The results show that the treatment combined a flight velocity of 2 m s-1 and an application rate of 60 L ha-1 obtains the most droplets among all the variables based on the values of droplet coverage and density. The treatment with a flight velocity of 2 m s-1 and an application rate of 75 L ha-1 has the best penetration, while the treatment with a velocity of 2 m s-1 and an application rate of 60 L ha-1 takes the second place according to the percentages of droplet deposition in the three layers. Overall consideration of the total droplet distribution and penetration of the application parameters, a flight velocity of 2 m s-1 and an application rate of 60 L ha-1 are recommended to get an ideal droplet distribution in tree canopy when UAV-based sprayer T30 equipped with fruit tree mode flies at 1.6∼2 m above the citrus tree canopy.