Excessive nitrogen inputs from human activities have become the main cause of water eutrophication and related ecological hazards. In order to study the impact of human activities on nitrogen in the basin, and based on statistical data of administrative units in 16 towns and villages, this study used the NANI model to calculate net anthropogenic nitrogen inputs (NANI) at township scale in Erhai Lake basin. Results show that the total amount of NANI in Erhai Lake basin was 29.81×103 t in 2014, and nitrogen input intensity per unit area was 10986 kg·(km2·a)-1, significantly higher than the national average. The input of nitrogen from food by the local tourist population was 0.26×103 t, accounting for 8% of local food nitrogen input. Nitrogen input from chemical fertilizer is the largest NANI input source, accounting for 47% of net nitrogen input in the basin, followed by net nitrogen input of food and feed. The spatial distribution of NANI at township scale shows evident regionalization, with higher values in the north and lower values in the south of the basin. The intensity of NANI in towns with cropland or population is high. The corresponding risk of nitrogen pollution in Erhai Lake basin is therefore a primary concern, and will remain so in the near future.