ABSTRACT The current study fractionated pollution sources and investigated the impacts of seasonal and tidal variations on Saigon River’s surface water quality. Ninety-six water samples were collected across dry and rainy seasons, covering ebb and flood tides, and analysed for 19 parameters. During the dry season, ebb tide showed higher levels of electrical conductivity, sulfate, chloride, sodium, and potassium. During the rainy season, ebb tide exhibited greater concentrations of ammonium, total nitrogen, and phosphorus. The water quality index was significantly lower during the dry season’s ebb tide compared to the flood tide but remained similar in both tidal phases during the rainy season. Four primary pollution sources were quantified, with seawater intrusion and agricultural activities contributing 62.68% and 28.54%, respectively, to water quality degradation. Briefly, the river’s surface water quality was inferior during the dry season compared to the rainy season, primarily due to seawater intrusion and agricultural activities, requiring remediation.