Insukamini catchment depends on hand dug wells, sand water abstraction, dam, and borehole water sources for their domestic uses. On hand dug wells and sand water abstraction, people rely on natural filtration of bacteria and consider the water as safe, however the location and management of some of these water sources raise suspicion of possible contamination. The aim of this study was to assess drinking water quality in terms of selected physico-chemical and microbiological parameters, and people's perceptions on aesthetic parameters. Water samples were collected from five water sources, namely sand water abstraction, dam, hand dug well, borehole and municipal water. Three samples were collected from each water source to make 63 samples. Temperature, pH, electrical conductivity, and dissolved oxygen measured using HI 9143 dissolved oxygen meter, YSI pH 100, HI 9143 dissolved oxygen meter and HI 8633 conductivity meter respectively. On laboratory experiments total suspended solids measured using filter disk, total dissolved solids using gravimetric method (SA, 2008), total and feacal coliform were measured using spread plate method. All water sources had total coliform levels above the WHO standards for drinking water of 1000mg/L. For E. coli, borehole and municipal water sources complied with the WHO standard with the means of zero cfu/ 100ml. Low pH mean of 5.001 was found in borehole water and the other water sources were within the accepted range of 6.5 to 8.5. Municipal and dam point (A) water sources had highest levels of TDS, borehole, and dam point (C) had TSS levels above maximums allowable of 1000mg/L and 150mg/L respectively. Temperature, DO and EC of all water sources were within the WHO standards. Overall, all water sources were not complying with WHO standard for drinking water. People described municipal water source as best for drinking purpose, dam, sand water abstraction and hand dug well water sources described as soft and good for laundry and bathing as compared to borehole which was described as hard. The results indicated that water from the sources under study were not suitable for drinking purposes without treatment. The water should rather be used for other domestic uses such as laundry and bathing. However, municipal water should be boiled or use water purification tablets (chlorine tablets) before drinking. An integrated approach is therefore required to minimize and avoid water pollution within the catchment.