The assessment of the ratio of self-purification processes in the natural environment, including the water bodies of streams and rivers is possible by the means of the numerical modelling. Nonetheless, the reliability of results of the qualitative numerical calculations may be affected by the quality of the collected input data and efficiency of the model calibration. The commonly required input data include pollutants characteristics and coefficients for empirical equations of their transport and decay, while calibration of a model requires series of measurements of selected pollutants concentration in water. This paper presents studies of concentration of pollutants measured in the treated sanitary wastewater discharged to the Bystrzyca river from municipal wastewater treatment plant, and their concentrations measured directly in the river, above and below the location of discharge point. The performed qualitative measurements of treated sewage discharged from municipal wastewater treatment plant and water of the Bystrzyca river included determination of COD, BOD5, N-NH4, N-NO2, N-NNO3, TKN, TN, TP and TSS concentration. The presented results of measurements are the first step in development and calibration of the model, allowing to successfully predict the influence of discharged sanitary wastewater on quality of water in the river.