AbstractAimModern pollen assemblages provide a means to calibrate fossil pollen data and to provide a translation from habitat and vegetation type to pollen representation. Here we provide a database of modern pollen abundances from a broad range of neotropical habitats and locations.LocationThe Neotropics, especially western Amazonia, the Andes, The Galápagos, Central America, Puerto Rico and Mexico.TaxonAngiosperms and GymnospermsMethodsOver a 30‐year period, mud–water interface samples, moss polsters, soils and pollen traps were used to assay modern pollen assemblages. Standard extraction methods were used, and a minimum of 300 terrestrial pollen grains were counted and identified in each sample. Bioclimatic data were assigned to each location, and sexual traits and pollination syndromes are provided for some genera. A detrended correspondence analysis (DCA) shows the structuring of the data relative to mean annual temperatures (MAT), mean annual precipitation (MAP) and precipitation of the driest quarter (PDQ). Probability density functions of distances among samples are calculated within and between Holdridge Life Zones, based on sample DCA scores.ResultsThe modern pollen of 636 locations are documented, with >500 pollen types identified. The dataset spans settings with MAT <5°C to > 25°C, and with MAP ranging from c. 500 to 5,000 mm, and PDQ from 80 to 1,500 mm. Ordination of the dataset provides evidence that assemblages differ according to temperature and precipitation, although historical biogeography also shapes pollen rains. The pollen grains of zoophilous species contribute important information to habitat descriptions, but they are under‐represented in the dataset.Main conclusionsThe dataset covers a broad environmental range and can help inform biogeographical and palaeoenvironmental interpretation of palaeoecological records. Some caution in using the data is needed as the field of tropical palynology is maturing and the data gathered 30 years ago are less detailed than those gathered recently. Although anemophilous taxa tend to be over‐represented in neotropical pollen spectra, the majority of pollen identified in each sample are from zoophilous species.