Atmospheric vapor pressure deficit (VPD) is the driving force that regulates the rate of water transport within plants. Under High VPD (HVPD), plants always reduce their photosynthesis rate and close their stomata. Experiments were performed under greenhouse conditions with cucumber and tomato plants to identify the regulatory effect of VPD on plant water capacity. Treatments included two levels of soil water (100% and 60% field capacity [FC]) combined with two levels of VPD (LVPD and HVPD). Results indicated that with 60%FC, the plant heights of tomato and cucumber were enhanced under LVPD compared with those under HVPD. With 60%FC, relative leaf water contents under LVPD increased by 11% compared with those under HVPD. Furthermore, LVPD significantly improved the photosynthetic capacity of the two crops and changed their stress responses. Our results indicated that LVPD at different soil moisture levels reduced irrigation demand under greenhouse conditions. This approach can be applied in water management in greenhouse vegetable production in China and other regions of the world with temperate continental climates.