Karst ecosystems offer a wealth of ecosystem services, but their protection is increasingly challenging due to degradation and land-use conversion, including limestone mining. This study investigates the water provision service derived from karst ecosystems threatened by limestone mining in the Watuputih groundwater basin, Central Java, Indonesia. Water supply was quantified by measuring water discharge rates from major springs around the basin. Water usage was quantified for household consumption, agriculture, and recreation. This study also measured water infiltration rates in mining and non-mining areas to indicate the effects of limestone mining to hydrological processes. This study identified three primary springs around the basin: Sumber Seribu spring, Brubulan spring, and Kalutan spring, with discharge rates of 1,080 liters per second, 85 liters per second, and 0.76 liters per second, respectively. A local water company extracts water from Sumber Seribu spring at a rate of 80 liters per second to supply approximately 57,600 individuals. The water supply from the three springs can irrigate approximately 1,594 hectares of rice fields. Water from Sumber Seribu spring also supports recreational activities in Sumber Semen recreation park, attracting an average of 19,173 visitors per year. This study revealed a complete impairment of soil's water infiltration capacity in limestone mining areas. These findings underscore the pressing need to safeguard the Watuputih groundwater basin and to mitigate the detrimental effects of limestone mining on hydrological processes. This involves designating the Watuputih karst ecosystem as a protected area, implementing Payment for Ecosystem Services (PES) programs, and restoring degraded post-mining sites.