Aquaculture Water Research Articles

Comparative pathogenicity and histopathological analysis of Edwardsiella anguillarum intraperitoneal infection in milkfish (Chanos chanos), Nile tilapia (Oreochromis niloticus) and Asian seabass (Lates calcarifer).

Edwardsiella anguillarum, a highly virulent species within the Edwardsiella genus, causes significant mortality in milkfish farms in Taiwan. This study aimed to investigate the comparison of milkfish susceptibility, a newly identified host species in Taiwanese aquaculture, with other species Nile tilapia (Oreochromis niloticus) and Asian seabass (Lates calcarifer), to E. anguillarum, elucidating its pathogenicity across both seawater and freshwater aquaculture environments. The results showed milkfish exhibited the highest mortality rate of 85% within 48 h of infection, whereas Nile tilapia exhibited a mortality rate of 70% between the second- and tenth-day post challenge, and seabass exhibited a mortality rate of 25% between the second- and sixth-day post challenge. Gross lesions observed in milkfish included splenomegaly and haemorrhage, whereas Nile tilapia exhibited signs of ascites, exophthalmia and brain haemorrhage. Seabass displayed spleen granulomas and haemorrhage at the injection site. Histopathological analysis revealed common features across all three species, including multifocal necrosis, bacterial presence in the necrotic areas, serositis and oedema. Asian seabass also exhibited chronic lesions in the form of splenic granulomas. This study highlights the high susceptibility of milkfish and Nile tilapia to E. anguillarum, emphasizing the urgent need for further investigation into targeted vaccine development for these fish species. These results not only deepen our understanding of the differing levels of pathogenicity among the three species but also offer valuable insights for improving disease prevention and management strategies in aquaculture, including those applied within polyculture systems and for the maintenance of aquaculture water environments.

Study of Life Feed in Cantang Grouper Hatcheries (Epinephelus fuscoguttatus x Epinephelus lanceolatus)

Abstract Cantang grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus) is a cross between female tiger grouper (Epinephelus lanceolatus) and male tiger grouper (Epinephelus fuscoguttatus), according to statistical data from the Ministry of Maritime Affairs and Fisheries (KKP) it has a production value of 4,406 tons in 2022 and experienced an increase in growth of 33.92%. Fulfilling this need can be done by maximizing the hatchery process, the key to which is natural food, namely for broodstock (flying fish, tuna and squid) and larvae (chlorella, artemia, rotifers, rebon shrimp). The aim of this article is to study natural feed in the hatchery of cantang grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus). This study was conducted at the Pecaron Brackish Water Aquaculture Fisheries Center (BPBAP), Situbondo, East Java on 19 June - 19 August 2023. The work method used was a descriptive method with primary and secondary data collection. The study results showed that the Hatching Rate (HR) was 73.25%, the Survival Rate (SR) was 15.98%, the Specific Grow Rate (SGR) was 0.24 g/day. Water quality measured is temperature ranging from 28-30°C, pH between 7.78-8.44, salinity between 31-32 ppt, dissolved oxygen (DO) between 5.72-6.20 mg/L, ammonia between 0.001-0.031 mg/L, nitrite is 0.001 mg/L. Natural feed has complete amino acids, fatty acids, enzymes and minerals which function in the maturation of the parent gonads and larval growth.

Exploring the variations in molecular characteristics of dissolved organic matter driven by aquaculture types

In recent decades, global aquaculture has expanded rapidly, raising concerns about coastal environmental degradation due to unregulated or poorly regulated discharge of aquaculture tailwater. Despite the crucial role of dissolved organic matter (DOM) in biogeochemical processes and aquatic biodiversity, the influence of aquaculture type on the molecular characteristics of DOM remains largely unexplored. Herein, this study investigated the variations in chemical and spectroscopic properties as well as molecular characteristics and composition of DOM across different aquaculture types including crustacean, fish and shellfish. Our findings revealed notable differences in DOM quantities among different aquaculture types, with crustacean and fish aquaculture water containing higher DOM amount compared to shellfish aquaculture water. This disparity can be attributed to the more frequent formulated feeds of crustacean and fish in contrast to shellfish aquaculture. Furthermore, distinct differences were also observed in the characteristics and composition of DOM among the different aquaculture waters. Specifically, DOM in shellfish aquaculture water exhibited a higher abundance of unsaturated and reduced molecules as well as increased aromaticity compared to the other two aquaculture waters. Conversely, DOM from fish aquaculture water showed a greater contribution from terrestrial origin characterized by elevated levels of plant-based components such as lignin-like and tannin-like compounds. Interestingly, DOM from shellfish aquaculture water contained lower levels of microbial-derived components such as lipid-like and protein-like compounds, likely due to reduced microorganism populations resulting from lower nutrients availability and higher salinity. Overall, these significant variations in characteristics and composition of DOM underscore the potential impacts of aquaculture type on the DOM biogeochemical cycle and the environmental quality in aquatic ecosystems.

Detection and mitigation of soil salinization risk from saline/brackish water aquaculture in coastal areas: an application of remote sensing and managed aquifer recharge

This study focuses on detecting and mitigating soil salinization in four coastal areas of the Mekong Delta (Vietnam). Salinity patterns in the soils of the Mekong River Delta are not random but linked to land use practices and distance to the sea. We examine two quick yet reliable remote sensing-based techniques to detect the coastal aquaculture area and separate it from the inland freshwater farmland. These techniques can eventually be used to identify locations with an elevated risk of salinization in other coastal regions. Finally, we investigate a salinization mitigation solution based on creating a managed aquifer recharge system along the buffer zone that separates the coastal aquaculture area from the inland freshwater agriculture area. The implementation of an infiltration pond system is technically feasible in the Mekong Delta provided that hydrogeological characteristics, the fresh-saline interface position, and freshwater demands are considered. The transitional zone between freshwater agriculture and brackish water aquaculture in Bac Lieu, Soc Trang, Tra Vinh and Ben Tre provinces is optimal for implementing an aquifer recharge/freshwater barrier scheme.

Hydrogel‐Based Capacitive Sensor Model for Ammonium Monitoring in Aquaculture

Monitoring aquaculture water quality, especially ammonium levels affecting fish health, is challenging due to limitations in traditional methods. This study presents an innovative capacitive sensor with an ionic hydrogel transducer for real‐time ammonium concentration monitoring. The sensor operates through ammonium‐induced dissociation in the hydrogel, causing osmotic pressure changes and altering electrode capacitance. Analytical formulations verified by finite element simulations demonstrate the sensor's ability to detect varying ammonium levels. Detailed analysis covers diffusion kinetics, swelling behavior, stress distributions, voltage variations, and ion equilibrium concentrations. The findings show a 20% increase in capacitance change within 200 s, even with a 3 μM ammonium concentration exposure. Additionally, the capacitance change plateau at ammonium levels exceeding 1 mM. The presented concept offers a promising and cost‐effective approach to addressing the limitations of traditional methods, paving the way for future research and development in real‐time water quality monitoring in aquaculture systems.

Development and evaluation of an Arduino-based multi-sensor aquaculture water quality monitoring kit

Development and evaluation of an Arduino-based multi-sensor aquaculture water quality monitoring kit

Teknik Pembenihan Ikan Kerapu Cantang (Ephinephelus sp.) di Balai Budidaya Air Payau, Situbondo, Jawa Timur

Grouper fish (Epinephelus sp.) is one of the popular marine fish species in the market in Indonesia and abroad in Southeast Asia, this fish has high economic value, good taste. Currently, the development of grouper aquaculture has been growing, so the seeds must be available continuously to meet the demand for seeds, therefore the foundation of interspecies crosses between groupers that have a high heterosis effect in growth traits, disease resistance and environmental resistance. This research activity was carried out at the Brackish Water Aquaculture Center located in Situbondo, East Java and was carried out from June 19, 2023 to August 19, 2023. The working method used in the Field Work Practice is descriptive method with data collection including primary and secondary. The technique of grouper hatchery conducted at the Brackish Water Aquaculture Fisheries Center stages include tub preparation, broodstock rearing, hybridization, hatching and egg stocking, larval development, feeding, water quality management, growth sampling and grading, pests and diseases, and harvesting and marketing. The results of the observed parameters are HR 73.25%, SR 15.98%, FR 74.16%. Water quality management carried out in grouper hatcheries is water reduction and water changes according to larval age, siphon once a week and water quality tests.

Effects of cadmium on the growth, muscle composition, digestion, gene expression of antioxidant and lipid metabolism in juvenile tilapia (Oreochromis niloticus)

Cadmium could induce various degrees of harm to aquatic organisms. A 30-day feeding trial was conducted to investigate the effects of cadmium on growth, muscle composition, digestive enzyme activity, gene expression of antioxidants and lipid metabolism in juvenile genetic improvement of farmed tilapia (GIFT, Oreochromis niloticus, Initial weight: 21.36 ± 0.24 g). Four cadmium concentrations of aquaculture water were designed: 0, 0.2, 0.4, and 0.6 mg/L Cd2+. The main results are as follows: Compared with the control group (0 mg/L Cd2+), the weight gain rate (WGR), specific growth rate (SGR), daily growth index (DGI), and spleen index (SI) of juvenile GIFT under cadmium stress were significantly decreased (p< 0.05). The contents of crude protein and crude lipid in muscle were significantly decreased (p< 0.05), and the ash was significantly increased (p< 0.05). The activities of trypsin, lipase, and α-amylase in the intestinal were significantly decreased (p< 0.05). The relative expression levels of carnitine palmityl transferase 1 (cpt-1), peroxisome proliferator-activated receptor α (pparα), pparγ, hormone-sensitive lipase (hsl), lipoprotein lipase (lpl), malate dehydrogenase (mdh), leptin (lep), fatty acid synthetase (fas), cholesterol response element binding protein 1 (srebp1), squalene cyclooxygenase (sqle), and stearoyl-CoA desaturase (scd) genes in liver were significantly decreased (p< 0.05). The relative expression levels of catalase (cat), superoxide dismutase (sod), glutathione S-transferase (gst), and glutathione peroxidase (gsh-px) genes in the liver were significantly decreased (p< 0.05). In conclusion, exposure to cadmium stress could impact growth, muscle composition, digestive enzyme activity, gene expression of antioxidant and lipid metabolism in juvenile GIFT.

POTENTIAL OF BIOFLOC TECHNOLOGY IN AQUACULTURE WASTEWATER TREATMENT

The increasing global demand for aquatic products and decline in wild fisheries pose a challenge in achieving the United Nations’ Sustainable Development Goal 14, which is to conserve and sustainably use marine resources. The depletion of fish populations due to overfishing, destruction of aquatic habitats as well as climate change has adversely affected aquatic ecosystems, which leads to further pressure in establishing food security. To meet the rising demand for fish products, countries have turned to aquaculture, but the industry itself faces many environmental challenges, particularly in wastewater management. This review explores the potential of using biofloc technology (BFT) to treat wastewater. BFT utilises microbial ecosystem processes to remove excess nutrients and acts as a natural “cleaning” mechanism. It transforms organic waste into valuable microbial biomass, which enhances water quality and minimises the ecological footprint of aquaculture. In this way, BFT reduces the amount of solid waste generated, increases the level of dissolved oxygen and creates an environment that is less conducive for the growth of harmful bacteria, thus reducing the need for chemical treatments. This paper also discusses the role of BFT in toxic remediation by analysing the nature and composition of aquaculture wastewater. This study provides a comprehensive overview of the mean values for various water quality parameters in aquaculture and biofloc water, and compares them with aquaculture standards.

Isolation of Trichoderma strains from rhizospheric soil and assessment of their potential for biofertilizer from freshwater aquaculture pond sediment

Abstract. Thi QVC, Nhan TH, Hung NVK, Thuy NP. 2024. Isolation of Trichoderma strains from rhizospheric soil and assessment of their potential for biofertilizer from freshwater aquaculture pond sediment. Biodiversitas 25: 2866-2876. Trichoderma spp. are filamentous fungi found in almost all soils and can synthesize massive quantities of cellulase enzymes which can simply break down cellulose polysaccharides. Freshwater water aquaculture pond sediment has caused some environmental pollution which is required to be properly dealt with. The objective of this study was to isolate Trichoderma strains from rhizosphere soil and to evaluate their potential for biofertilizer from freshwater aquaculture pond sediments. Twenty-six strains of Trichoderma spp. were isolated from soil. The Carboxymethyl Cellulose (CMC) degrading ability of the S25 strain was extremely strong, with a halo diameter zone measuring 8.9±3.8 cm. According to the BLAST result, there was 99.11% similarity between S25 strains and Trichoderma asperelloides, with a max score of 1011 and an E-value of 0.0. The T. asperelloides S25 was selected for bioproduct production. The use of T. asperelloides S25 bioproduct passively enhanced the efficient decomposition of organic fertilizers. After just 30 days of incubation, total organic matter content was ? 30% and material had a spongy, soft, and brown-black in color. According to the Government of Vietnam’s Decree No. 14/2019/ND-CP on fertilizer management, dated September 14, 2019, all treatments were chosen for the production of granular bio-compost. These findings highlight the potential of bioproduct T. asperelloides S25 for sustainable aquaculture practices and organic waste management.

A multi-model ensemble approach for reservoir dissolved oxygen forecasting based on feature screening and machine learning

Dissolved oxygen (DO) concentration in aquatic systems plays a vital role in water aquaculture. An innovative approach that combines feature selection and ensemble learning to predict DO in aquatic ecosystems was proposed. Feature selection was first performed using Maximum Information Coefficient (MIC). Five machine learning algorithms were then employed to construct five hybrid-MIC models, including K-Nearest Neighbors (KNN), Backpropagation (BP) Neural Network, Long Short-Term Memory (LSTM), Kernel Ridge Regression (KRR), and Support Vector Regression (SVR). Finally, an ensemble-RF prediction model was built using Random Forests(RF). The main findings are as follows: (1) The MIC technique can effectively identify the key factors influencing DO. (2) The MIC significantly improves model performance. (3) The hybrid-MIC model was further improved by the ensemble-RF model, the average R2 and NSE were both as high as 0.99, and the average MAE and RMSE were decreased by 72 % and 64 %, respectively.

Spatiotemporal dynamics, bioaccumulation, and critical influencing factors of antibiotics in tilapia aquaculture: A study on source identification and environmental fate within typical farming systems

This study investigated the spatiotemporal dynamics, bioaccumulation, and critical influencing factors of antibiotics in tilapia aquaculture, focusing on source identification and environmental fate within typical farming systems. The results revealed a progressive increase in antibiotic concentrations in pond water and sediments over the cultivation period, with suspended solids and chemical oxygen demand identified as significant environmental factors influencing the distribution and dissemination of antibiotics. The aquaculture water source was the primary contributor of antibiotics in the farming system. Furthermore, the bioaccumulation factor (BAF) calculations indicated varying degrees of antibiotic enrichment in tilapia tissues, with sulfadimethoxine exhibiting the highest BAFs. Correlation analyses, redundancy analysis, and multivariate linear regression analysis provided insights into the relationship between environmental factors and antibiotics, identifying key antibiotics and influencing factors. The study highlighted the importance of managing and treating water sources to reduce the inflow of antibiotics into aquaculture systems and emphasized the need for non-antibiotic aquaculture practices to minimize the impact on the environment and public health. In conclusion, this research contributes valuable information for the development of effective management strategies and policies aimed at curbing antibiotic pollution in aquaculture environments, ensuring the sustainability of the aquaculture industry, and protecting ecosystem and consumer health.

Research progress on formation mechanism of pearl

Pearls are deeply cherished for their rich color and gorgeous luster, and their quality directly affects their value. Currently, the evaluation of pearl quality is mainly based on four aspects: color, shape, size and smoothness. The quality of pearls is influenced by a variety of factors, categorized into internal factors, such as the structural composition of the nacreous layer and genetic factors of the mussels, and external factors, including the aquaculture environment. Existing research results indicates that genetic factors are the dominant factor controlling the pearl quality. However, the macromolecules such as metal ions, organic pigments and various physical and chemical factors in the aquaculture water environment will also significantly impact pearl quality. Among these, matrix proteins are organic macromolecules found in the nacreous layer that play an important role in pearl quality. They participate in the deposition of calcium carbonate and the construction of the organic framework, affecting the pearls’ size and shape. The color of pearls is influenced by the deposition of metal ions, the transport of organic pigments and the regulation of microstructure.

The dynamics of catching aquatic bioresources in the south of Ukraine: Analysis, challenges and prospects for their solution in the context of sustainable development

Given the significance of fish farming as a promising sector of Ukraine’s agriculture, and the Mykolaiv, Odesa, and Kherson regions are characterised by a significant number of natural and artificial water bodies, the study of aquatic bioresource catching indicators in the south is both important and timely. The research aimed to determine trends in aquatic bioresource catching in southern Ukraine during the period 2018-2023 and to analyse the factors influencing the extraction of aquatic bioresources. This article presents overall catch statistics, as well as catch data for inland waters of the south and aquaculture conditions over the past six years. It has been established that the catch of aquatic biological resources in southern Ukraine has been declining each year from 2018 to 2023. The negative trends in aquatic bioresource catching, both in aquaculture and natural water bodies, are linked to several factors. In particular, the decline in catches has been influenced by: COVID-19; the unstable military and political situation in Ukraine, associated with Russian military aggression; outdated material and technical base of fishing enterprises; lack of government subsidies and foreign investment in the sector; imperfections in the systems of control and regulation of the extraction of aquatic bioresources; and irrational use of the bioproductive potential of water bodies in southern Ukraine. Based on a scientific analysis of the dynamics of aquatic bioresource extraction in southern Ukraine and the identification of key factors contributing to the decline in catches, a set of measures has been developed to positively impact the fishing industry. These measures include: attracting foreign investment in fish farming; improving the quality of monitoring, regulation, and control over aquatic bioresource catching at local, regional, and state levels; restoring critical fish farming infrastructure damaged by Russian aggression; ensuring sustainable fisheries management; and introducing EU best practices into Ukrainian fish farming. The scientific results obtained from this research can be used in practice to ensure the sustainable development of fisheries in southern Ukraine

Coupling Imports of Dissolved Inorganic Nitrogen and Particulate Organic Matter by Aquaculture Sewage to Zhangjiang Estuary, Southeastern China

Estuary ecosystems serve as crucial connectors between terrestrial and marine environments, thus playing vital roles in maintaining the ecological balance of coastal marine ecosystems. In recent years, the eutrophication in estuaries caused by aquaculture sewage has been revealed, highlighting the necessity to understand its influence on the nutrient conditions and carbon storage of estuaries. In this study, δ15N and δ18O were used to indicate the contribution of aquaculture-derived sewage to dissolved inorganic nitrogen in Zhangjiang Estuary, and δ13C and C:N ratio were used to reveal its effects on the particulate organic matter. The major results are as follows: (1) Aquaculture water contributed 62~86% and 60~100% of the total nitrate and ammonium in Zhangjiang Estuary, respectively, and the drainage periods of the cultured species has a great influence on the content and composition of dissolved inorganic nitrogen. (2) Aquaculture water was also the major source of particulate organic matter (24~33% of the total content) here, most of which may be derived from crab ponds. (3) The imports of nutrients by aquaculture water may potentially regulate particulate organic matter in Zhangjiang Estuary by promoting the growth of phytoplankton and zooplankton. Our study revealed the coupling effects of aquaculture activities on the nitrogen and carbon storage in an estuarine ecosystem. It also indicates that isotopes may be efficient in the monitoring of a coastal environment, which may further aid the management of inshore cultivation.

Combined Electrochemical Deposition and Photo-Reduction to Fabricate SERS-Active Silver Substrates: Characterization and Application for Malachite Green Detection in Aquaculture Water.

This research introduces a novel approach using silver (Ag) nanostructures generated through electrochemical deposition and photo-reduction of Ag on fluorine-doped tin oxide glass substrates (denoted as X-Ag-AgyFTO, where 'X' and 'y' represent the type of light source and number of deposited cycles, respectively) for surface-enhanced Raman spectroscopy (SERS). This study used malachite green (MG) as a Raman probe to evaluate the enhancement factors (EFs) in SERS-active substrates under varied fabrication conditions. For the substrates produced via electrochemical deposition, we determined a Raman EF of 6.15 × 104 for the Ag2FTO substrate. In photo-reduction, the impact of reductant concentration, light source, and light exposure duration were examined on X-Ag nanoparticle formation to achieve superior Raman EFs. Under optimal conditions (9.0 mM sodium citrate, 460 nm blue-LED at 10 W for 90 min), the combination of blue-LED-reduced Ag (B-Ag) and an Ag2FTO substrate (denoted as B-Ag-Ag2FTO) exhibited the best Raman EF of 2.79 × 105. This substrate enabled MG detection within a linear range of 0.1 to 1.0 µM (R2 = 0.98) and a detection limit of 0.02 µM. Additionally, the spiked recoveries in aquaculture water samples were between 90.0% and 110.0%, with relative standard deviations between 3.9% and 6.3%, indicating the substrate's potential for fungicide detection in aquaculture.

A novel nitrogenous coumarin: The promising antiviral therapy against white spot syndrome virus in the culture of shrimp seedlings

White spot disease (WSD), attributed to the white spot syndrome virus (WSSV), represents a substantial danger to the international shrimp sector, resulting in considerable losses in both production and economic terms. Nevertheless, there are currently no approved therapeutic measures to control the outbreak. This study involved the synthesis of 15 new compounds for the assessment of their anti-WSSV efficacy in Litopenaeus vannamei post-larvae. Among these compounds, N-(4-methyl-2-oxo-2H-chromen-7-yl)acetamide (P13) exhibited a maximum antiviral response of > 90 %, with a median effective concentration (EC50) < 12 mg/L, making P13 the most promising antiviral compound. In the cases where the larvae were simultaneously incubated with WSSV and P13, as well as where the larvae were first infected with WSSV and then treated with P13, the replication of WSSV was significantly inhibited. However, pre-treatment with P13 had minimal impact on WSSV multiplication, suggesting a potential therapeutic effect. Simultaneously, the cumulative mortality rate of WSSV-infected post-larvae decreased in the presence of P13. After the shrimp larvae were infected with WSSV, continuously changing the aquacultural water containing DMSO resulted in a mortality rate of 80 % at 120 hours post-infection (hpi); whereas continuously changing the aquaculture water containing P13 resulted in a mortality rate of 45 %. Given that P13 remains stable in farmed water for up to two days, it is recommended to regularly replace the medication to eliminate the virus threat. In conclusion, P13 demonstrates a positive anti-WSSV activity and holds potential for further development as an antiviral therapy against WSSV infection.

Photodegradation of bisphenol A (BPA) in coastal aquaculture waters: Influencing factors, products, and pathways

Bisphenol A (BPA), an endocrine-disrupting contaminant, is ubiquitous in the environment due to its presence in plastics, wastewater, and agricultural runoff. This study investigated the photodegradation behavior of BPA in coastal aquaculture waters near Qingdao, China. Lower salinity promoted BPA photodegradation, while higher salinity has an inhibitory effect, suggesting slower degradation in seawater compared to ultrapure water. Triplet-excited dissolved organic matter (3DOM*) was identified as the primary mediator of BPA degradation, with additional contributions from hydroxyl radicals (•OH), singlet oxygen (1O2), and halogen radicals (HRS). Alepocephalidae aquaculture water exhibited the fastest degradation rate, likely due to its high DOM and nitrate/nitrite (NO3−/NO2−) content, which are sources of 3DOM* and •OH. A positive correlation existed between NO3−/NO2− concentration and the BPA degradation rate. Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) analysis identified the primary BPA photodegradation products, formed mainly through oxidative degradation, hydroxyl substitution, nitration, and chlorination pathways. Elucidating these photodegradation mechanisms provides valuable insights into the environmental fate and potential ecological risks of BPA in aquaculture environments. This knowledge can inform strategies for marine environmental protection and the development of sustainable practices.

Nitrogen and phosphorus budgets of the bullfrog (Aquarana catesbeiana) in different body weights

Aquaculture water eutrophication is primarily driven by nitrogen (N) and phosphorus (P), partly derived from animal excretions. Understanding the N and P metabolism is crucial for mitigating environmental pollution caused by aquaculture. This study investigated N and P budgets in bullfrogs (Aquarana catesbeiana) of varying body weights: 52.63 ± 0.33 (50 g), 98.64 ± 0.55 (100 g), 159.97 ± 0.58 (150 g) and 250.01 ± 2.61 (250 g). Our findings highlighted the significant influence of body size on N and P budgets in bullfrogs. As body weight increased, feed intake rate, N and P intake rates, growth rate, N and P growth rates, N and P metabolism rates, feces rate, while the N and P feces rates all decreased. The relationship between each index and body weight was expressed as a power function (Y = aWb). The N and P budget equations for bullfrogs were established as follows: 100 Intake N (IN) = 30.75 Growth N (GN) + 62.48 Metabolism N (MN) + 2.10 Feces N (FN) + 4.67 Others (ON), and 100 P Intake P (IP) = 74.16 Growth P (GP) + 4.18 Metabolism P (MP) + 7.92 Feces P (FP) + 13.75 Others (OP). In conclusion, daily feeding per body weight of bullfrog should be reduced as their body weight increased. Consequently, the dietary protein should be progressively lowered to minimize nitrogen waste, while bullfrogs demonstrated effective utilization of P in the soybean meal diet.

Monitoring Water Quality Parameters in Aquaculture Using Edge Computing

In this paper, we study an integration of temperature and pH sensors into the system to collect and monitor water quality parameters in aquaculture using a combination of edge computing (EC), Artificial Intelligence (AI) and Internet-of-Things (IoT). We develop and deploy a model using edge computing and a Long-Short Term Memory (LSTM) algorithm to forecast the aquaculture water quality at the network's edge. The system automatically operates and performs sampling, forecasting, and displaying predicting results on the user interface in real-time. The results of this paper demonstrate that the proposed solution with edge computing and LTSM can produce accurate forecasts with high reliability and prompt responses to water quality monitoring, thus reducing losses and harm due to water pollution in the aquaculture industry.