Water Extract Of Bark Research Articles

상백피 물 추출물의 항산화 활성 및 항천식 효과

This study was performed to evaluate the antioxidant activities and anti-asthma effects of Morus bark water extracts. Inhibitory effect of Morus bark onto free radical generation was determined by measuring DPPH and hydroxyl radical scavenging activities in vitro. Anti-asthma activities of Morus bark water extracts were assessed by testing their effects on the degranulation of mast cell. For this, β-hexosaminidase released from a basophilic cell line, RBL-2H3 was used and pro-inflammatory cytokines were measured by ELISA kit. The antioxidant activities of water extracts of Morus bark was 59.2% in the DPPH assay at 2,000 ㎍/mL and 78.8% in the hydroxyl radical scavenging assay at 2,000 ㎍/mL. Our results indicated that Morus bark water extracts effectively inhibited free radical generation. Morus bark water extracts inhibited inflammation-mediating substances such as histamine and β-hexosaminidase release from RBL-2H3 cells. Cytokine release demonstrated a more effective blockading ability of the Morus bark water extracts to the release of IL-4 and TNF-α compared to control. These results demonstrate that Morus bark may be beneficial in the treatment of allergic inflammatory disease.

Evaluating extracts of spondias mombin for antimicrobial activitie

The plant Spondias mombin Linn. also called yellow mombin in English, Igongo/Ichankla in Idoma, and Uchakuru in Igbo, is common in the forest and savanna regions of Nigeria. It is used in several countries of the world to treat various ailments including infectious diseases. Water, chloroform, methanol, and petroleum ether extracts of leaf, root, and bark of the mature plant were screened for antimicrobial activity using an indicator-based microdilution technique. Inhibition was measured as minimal inhibitory concentration (MIC). The growth of Streptococcus pyogenes (mean MIC = 0.139 mg), Candida albicans (mean MIC = 0.148 mg), Salmonella typhi (mean MIC = 0.226 mg), Escherichia coli (mean MIC = 0.265 mg), and Staphylococcus aureus (mean MIC = 0.289 mg) in broth cultures were inhibited. Inhibition was significantly correlated with plant parts (r = -0.435; p < 0.05), leaf extracts having the greatest inhibitory effect (mean MIC = 0.060 mg), and the bark extracts the least (mean MIC = 0.389 mg). Statistical tests show that the mean MICs of leaf and bark extracts differ significantly (p

Hypoglycemic and antihyperglycemic effect of different extracts of Acacia arabica lamk bark in normal and alloxan induced diabetic rats

Acacia arabica commonly known as babool are used in traditional Indian medicine for treatment of diabetes mellitus. The hypoglycemic effect of aqueous extract (hot and cold water) and hydroalcoholic extract of Acacia arabica was investigated. Oral administration of cold water extract of Acacia arabica bark to diabetic and normal rats at a dose of 400 mg/kg body weight resulted in significant reduction of blood glucose, cholesterol and triglycerides. Phytochemical investigations found that phenolic compounds are presents in Acacia arabica extracts. The cold water extract of Acacia arabica was found to reduce blood glucose level to its normal level with in seven days. Histological studies of the β-cells show its action on pancreas. Keywords: Diabetes, antidiabetics, Acacia arabica and babool.

Anti-inflammatory effects of the willow bark extract STW 33-I (Proaktiv®) in LPS-activated human monocytes and differentiated macrophages

Willow bark extract is frequently used in the treatment of painful rheumatological diseases, such as arthritis and back pain. Its effect has been attributed to its main component salicin, but pharmacological studies have shown that the clinical efficacy of the willow bark extract cannot be explained by its salicin content alone. Therefore different modes of action have been suggested for the anti-inflammatory effect of willow bark extract. Here, we report in vitro data revelling the effect and mode of action of the aqueous willow bark extract STW 33-I as well as a water-soluble fraction (fraction E [Fr E]) in comparison with well-known non-steroidal anti-inflammatory drugs (NSAIDs) like aspirin (ASA) and diclofenac (Diclo) on pro-inflammatorily activated human monocytes and differentiated macrophages. STW 33-I and the water-soluble Fr E showed concentration-dependent and significant anti-inflammatory effects in lipopolysaccharide-activated monocytes. Both inhibited the intracellular protein expression of tumour necrosis factor-alpha (TNFα) as well as the mRNA expression of TNFα and cyclooxygenase 2 (COX-2), and the release of nitric oxide (NO). In addition, apoptosis of pro-inflammatorily activated monocytes was induced. Furthermore, treatment of activated macrophages with STW 33-I inhibited the nuclear translocation of the p65 subunit of the nuclear transcription factor-kappa B (NF-κB p65). The present in vitro investigations suggest a significant anti-inflammatory activity of willow bark water extract STW 33-1 and of its water-soluble fraction by inhibiting pro-inflammatory cytokines (TNFα), COX-2 and nuclear translocation of the transcription factor NF-κB in pro-inflammatorily activated monocytes. Our results provide further evidence for the therapeutic use of STW 33-I in inflammation-related disorders.

In vitro effects of Warburgia ugandensis, Psiadia punctulata and Chasmanthera dependens on Leishmania major promastigotes.

Plant extracts from Warburgia ugandensis Sprague (Family: Canellaceae), Psiadia punctulata Vatke (Family: Compositae) and Chasmanthera dependens Hoschst (Family: Menispermaceae) were tested for activity on Leishmania major promastigotes (Strain IDU/KE/83 = NLB-144) and infected macrophages in vitro. Plants were collected from Baringo district, dried, extracted, weighed and tested for antileishmanial activity. Serial dilutions of the crude extracts were assayed for their activity against Leishmania major in cell free cultures and in infected macrophages in vitro. Inhibitory concentrations and levels of cytotoxicity were determined. Warburgia ugandensis, Psiadia punctulata and Chasmanthera dependens had an IC(50) of 1.114 mg/ml, 2.216 mg/ml and 4.648 mg/ml, respectively. The cytotoxicity of the drugs on BALB/c peritoneal macrophage cells was insignificant as compared to the highly toxic drug of choice Pentostam(®). The supernatants from control and Leishmania infected macrophages were analyzed for their nitrite contents by Griess reaction and nitrite absorbance measured at 540 nm. Warburgia ugandensis (stem bark water extract), Chasmanthera dependens (stem bark water extract) and Psiadia punctulata (stem bark methanol extract) produced 112.3%, 94% and 88.5% more nitric oxide than the untreated infected macrophages respectively. Plant crude extracts had significant (p<0.05) anti-leishmanial and immunomodulative effects but insignificant cytotoxic effects at 1mg/ml concentration. All experiments were performed in triplicate. Statistical analysis of the differences between mean values obtained from the experimental group compared to the controls was done by students't test. ANOVA was used to determine the differences between the various treatment groups. The analysis program Probit was used to determine IC(50)s.

Superoxide anion radical scavenging activity of Cassia siamea and Cassia javanica

This study was designed to explore the antioxidant potential of hexane, chloroform, ethyl acetate, methanol, and water extract of bark and leaves of Cassia siamea and Cassia javanica by superoxide anion radical scavenging assay. The different extracts showed significant inhibition of superoxide radicals in a dose-dependant manner. Among all the bark extracts of C. siamea, the methanol extract showed the maximum inhibition of 60.5% at 800 μg/ml concentration and water extract also showed strong antioxidant potential of 51.3% at 1000 μg/ml concentration. The water extract of bark of C. javanica showed strong antioxidant potential of 55% at 1000 μg/ml concentration. The various leaf extracts of C. siamea showed moderate antioxidant potential of 25–50% at 1000 μg/ml, whereas methanol leaf extract of C. javanica showed strong antioxidant potential of 50.4% at 300 μg/ml concentration. This preliminary study indicates the antioxidant activity of the bark and leaves of C. siamea and C. javanica.

Elucidation of Anti-inflammatory Potencies ofEucommia ulmoidesBark andPlantago asiaticaSeeds

This study elucidated possible mechanisms for the different anti-inflammatory potencies exhibited by the water extracts of Eucommia ulmoides bark and Plantago asiatica seeds, which contain various iridoids. Water extracts of both herbal materials were tested in vitro with a battery of assay models: lipopolysaccharide-induced thromboxane B(2) for cyclooxygenase-1 (COX-1), prostaglandin E(2) for cyclooxygenase-2 (COX-2), the translocation of nuclear factor-kappaB (NF-kappaB), and tumor necrosis factor alpha (TNFalpha) and nitric oxide (NO) production in RAW 264.7 cells. The contents of the iridoid glycosides, aucubin (AU), catalpol (CA), and geniposide (GE), were quantified by high-performance liquid chromatography (HPLC). Neither E. ulmoides nor P. asiatica suppressed the COX-1 enzyme. P. asiatica significantly inhibited COX-2 (concentration required for 50% inhibition [IC(50)] = 8.61 (microg/mL), TNFalpha (IC(50) = 9.63 (microg/mL), and NO (IC(50) = 8.65 (microg/mL) production. P. asiatica blocked the translocation of NF-kappaB from the cytosol to the nucleus. E. ulmoides suppressed only the COX-2 enzyme (IC(50) = 9.92 (microg/mL). The results of the HPLC analysis revealed that P. asiatica contained three iridoid glycosides, AU, CA, and GE. E. ulmoides contained CA and GE, but no AU was detected. The difference in the anti-inflammatory potencies of E. ulmoides and P. asiatica appears to be dependent on the presence of AU. Considering the IC(50) values, both herbal extracts exhibit modest and less potent anti-inflammatory activities than common synthetic non-steroidal anti-inflammatory drugs.

Antifungal activity of extracts and phenolic compounds from Barringtonia racemosa L. (Lecythidaceae)

The antifungal activity of methanolic, ethanolic and boiling water extracts of Barringtonia racemosa leaves, sticks and barks were investigate against Fusarium sp., Tricoderma koningii, Penicillium sp., Ganoderma tropicum, Ganoderma lucidum, Aspergillus sp. and Rhizopus sp. at concentration of 50 mg/ml. Better antifungal activity was observed with the methanolic extracts in all aerial parts of B. racemosa that showed excellent inhibitory activity against all the fungi tested. The strongest inhibitory activity effect was observed with the methanolic extract of leaf against Fusarium sp. (53.45%), G. lucidum (34.57%), Aspergillus sp. (32.27%) and T. koningii (20.99%). Remarkable are also the specific effects of the boiling water extract of leaf against Fusarium sp. (51.72%) and with the ethanolic extract of bark against Rhizopus sp. (37.50%). None of the boiling water extracts of leaf, stick and bark showed inhibitory activity effect against G. tropicum and T. koningii. Among different fungi tested, Fusarium sp. was found to be more sensitive to B. racemosa extracts when compared to others. The increase in the production of phenolics in the extracts can be correlated with the induction of resistance in treated plant against phytopathogenic fungi. HPLC analysis of the extract of B. racemosa (leaves, sticks and barks) showed two different phenolic acids (gallic acid and ferrulic acid) and four different flavonoids (naringin, rutin, luteolin and kaempferol). The results of present study provide scientific basis for the use of the plant extract in the future development as antifungal, antibacterial, antioxidant and antiinflammatory agent.

Comparative evaluation of successive extracts of leaf and stem bark of Albizzia lebbeck for mast cell stabilization activity

Successive chloroform, methanol and water extracts of bark and leaves of Albizzia lebbeck were tested for its in vitro mast cell stabilizing effect against compound 48/80. Methanolic extract of leaf and methanolic and water extracts of bark have shown maximum activity comparable to that of disodium chromoglycate.

두충 추출물이 DNCB로 유발된 알레르기성 접촉피부염과 산화적 손상에 미치는 영향

Inhibitory effects of allergic contact dermatitis of hot water extract of Duchung (Eucommia ulmoides Oliver) leaf, bark and stem growing at Sancheong-gun were investigated for female BALB/c mouse induced by repeat elicitation of DNCB (2,4-dinitro-chlorobenzene). Skin reactions, consisting of increased ear thickness and the presence of ear inflammation, were observed in mice treated with DNCB and Duchung. Weight of lymph node, spleen and thymus in mice treated with Duchung extracts were lower than that of mouse treated with DNCB. Ear weight of mouse treated with Duchung extracts was decreased by increasing the concentration of sample as compared to control group and dropped as low as control level at 1,000 ㎎/㎏. Ear thickness became thinner as test time on Duchung extract progressed. MDA (malondialdehyde) contents in liver tissue were not different in sample group with DNCB group, but were different in ear tissue. NO (nitric oxide) contents was decreased in Duchung extract groups at serum and ear tissues as compared to 1% DNCB group. In the present study, the results suggested that Duchung extract inhibits inflammatory response and oxidative damage induced DNCB allergen.

Effects of Sterculia tomentosa stem bark extract on haematological parameters and Trypanosoma brucei infection in rats

Evaluation of antitrypanosomal efficacy of stem bark water extract of Sterculia tomentosa using rats as a model revealed significant suppressive effect on parasitaemia with extended survival time in group D rats treated with extract subcutaneously. The extract did not affect packed cell volume of treated rats. There was no significant difference (p

Effects of Elm Bark (Ulmus davidianavar.japonica) Extracts on the Modulation of Immunocompetence in Mice

The immunomodulative effects of elm bark extract were studied in vitro by the proliferation of splenocytes and the production capacity of three kinds of cytokines [interleukin (IL)-1beta, IL-6, and tumor necrosis factor (TNF)-alpha] by mouse peritoneal macrophages cultured with various fractions (methanol, hexane, chloroform, ethyl acetate, butanol, and water) of elm bark extract. Splenocyte proliferation and cell viability of peritoneal macrophages were increased with concentrations of polar fractions, such as butanol and water, in the range of 1-500 microg/mL. Significantly higher levels of the production of all three cytokines were detected with supplementation of methanol extract compared with other fractions. In order to elucidate its effect in vivo, elm bark water extract was orally administrated every other day for 2 weeks. Proliferation of splenocytes and the production capacity of cytokines (IL-1beta, IL-6, and TNF-alpha) by mouse peritoneal macrophages were used as indices for immune activity. Splenocyte proliferation induced by elm bark with lipopolysaccharide or concanavalin A stimulation was enhanced at 500 mg/kg of body weight concentrations compared to that of the control group. In the case of cytokines, the highest production of IL-6 and TNF was detected at 500 mg/kg of body weight concentrations. In conclusion, this study suggests through in vitro and in vivo experiments that Ulmus davidiana var. japonica (elm bark) extracts may enhance the immunocompetent properties such as splenocyte proliferation and cytokine production capacity by activated macrophages and have a protective effect in mice.

In vitro macrophage activation by organic waste materials

The EU-wide ban on antibiotic growth promoters in animal production has stimulated research on functional feed additives that improve animal health and performance in the related industries. Organic wastes from industries like pharmaceutical or food production often have to be disposed at high costs and sometimes also cause environmental problems. The potential further use of such plant residues or by-products as value-added products in animal or human nutrition is currently evaluated in the course of the EU-funded project SAFEWASTES. Here we report on possible immunomodulating activity of several organic wastes by in vitro activation of a chicken macrophage cell line. Macrophages are important elements of the innate, non-specific and specific immune reaction of vertebrates. They represent a first-line defence against invading pathogens by phagocytosis, cytokine and nitric monoxide (NO) production and antigen presentation. In vitro activation of a chicken bone marrow-derived macrophage cell line (HD-11) was measured by NO production in a cell culture assay. Active substances were checked for lipopolysaccharide (LPS) contaminations caused by gram-negative bacteria that could lead to false-positive results. 52 extracts prepared from 18 different organic waste materials were investigated. Nine water extracts showed high macrophage activation comparable to the positive control, a bacterial cell wall preparation. Among them, willow bark water extract had no detectable amounts of LPS while they probably accounted for some activity of the other extracts. Bioassay-guided fractionation was done to further identify the active principles of the materials.

Changes in chromosome structure, mitotic activity and nuclear DNA content from cells of Allium Test induced by bark water extract of Uncaria tomentosa (Willd.) DC

The influence of water extract of Uncaria tomentosa (Willd.) DC bark on the meristematic cells of the root tips of Allium cepa L., e.g. cells of Allium Test, was investigated. The experiment was carried out in two variants: (1) continuous incubation at different concentrations (2, 4, 8 and 16 mg/ml) of the extract for 3, 6, 12, 24, 48 and 72 h; and (2) 24-h incubation in three concentrations of the extract (4, 8 or 16 mg/ml), followed by post-incubation in distilled water for 3, 6, 12, 24 and 48 h. During the continuous incubation, the mitotic activity was reduced (2 and 4 mg/ml) or totally inhibited (8 and 16 mg/ml), depending on the concentration of the extract. All the concentrations resulted in gradual reduction of the mitotic activity. In the concentration of 2 mg/ml, the mitotic activity reached its lowest value after 12 h (2 mg/ml) and after 24 h in 4 mg/ml, followed by spontaneous intensification of divisions during further incubation. Instead, in higher concentrations of the extracts (8 and 16 mg/ml), the mitotic activity was totally inhibited within 24 h and did not resume even after 72 h. Incubation caused changes in the phase index, mainly as an increase in the number of prophases. After 24 h of incubation, in all phases, condensation and contraction of chromosomes were observed. During post-incubation, divisions resumed in all concentrations, reaching even higher values than the control. Cytometric analysis showed that the extract caused inhibition of the cell cycle at the border between gap 2 and beginning of mitosis (G 2/M).

Effects of Pouteria cambodiana extracts on in vitro immunomodulatory activity of mouse immune system

Immunomodulatory activity of water and acetone extracts of stem bark of Pouteria cambodiana was examined on murine macrophage phagocytosis [nitroblue tetrazolium (NBT) dye reduction and lysosomal enzyme activity] and proliferation of splenocytes and bone marrow cells by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay with and without lipopolysaccharide (LPS) or pokeweed mitogen (PWM). Both aqueous and acetone extracts presented immunomodulatory activity without clear dose response relationship.

Effects of Elm Bark (Ulmus davidiana var. japonica) extracts on modulation of immunocompetence in mice

Ulmus davidiana var japonica (Elm bark) has been traditionally used for many medicine purposes as diuretic, laxative, or cure for insomnia and gastrointestinal disorders. Recently, its anti-inflammation response, antioxidant activity and anticancer activity have been reported. In order to elucidate its effect in vitro and in vivo, proliferation of splenocytes and the production capacity of cytokines (IL-1beta, IL-6 and TNF-alpha) by mice peritoneal macrophage were used as indices for immune activity. Splenocytes proliferation and cell viability of peritoneal macrophages was increased in the range of 1~500 §¶/§¢ and polar fractions such as butanol and water. Significantly higher levels of the production of all three cytokines were detected with supplementation of methanol fraction compared to those of other fraction. In vivo, Elm bark water extract was orally administrated every other day for 2 weeks. Splenocytes proliferation of Elm bark with LPS or con A stimulation was enhanced at 500 §¶/§¢ b.w concentrations compared to that of control group. In case of cytokine, the highest production of IL-6 and TNF-¥á was detected at 500 §·/§¸ b.w concentrations. In conclusion, this study suggests that Ulmus davidiana var japonica (Elm bark) extracts may enhance the immunocompetence such as splenocyte proliferation and cytokine production capacity by activated macrophages in mice through in vitro and in vivo experiments.

EFFECTS OF ULMUS DAVIDIANA PLANCH ON MINERALIZATION, BONE MORPHOGENETIC PROTEIN-2, ALKALINE PHOSPHATASE, TYPE I COLLAGEN, AND COLLAGENASE-1 IN BONE CELLS

Ulmus davidiana Planch (Ulmaceae) (UD) long has been known to have anti-inflammatory and protective effects on damaged tissue, inflammation, and bone among other functions. The herbal medicine also is being used in Oriental medicine to treat osteoporosis. In a preliminary study, treatment of osteoclasts containing long bone cells with the water extract of UD bark prevented the intracellular maturation of cathepsin K (cat K), and thus, it was considered that UD is a pro-drug of a potent bone-resorption inhibitor. To further clarify the role of UD in ossification, we investigated the effects of UD on the proliferation and differentiation of osteoblastic cell lines in vitro. In this study, we assessed the effects of UD on osteoblastic differentiation in nontransformed osteoblastic cells (MC3T3-E1) and rat bone marrow cells. UD enhanced alkaline phosphatase (ALP) activity and mineralization in a dose- and time-dependent fashion. This stimulatory effect of the UD was observed at relatively low doses (significant at 5-50 microg/ml and maximal at 50 microg/ml). Northern blot analysis showed that UD (100 microg/ml) increases in bone morphogenic protein-2 as well as ALP mRNA concentrations in MC3T3-E1 cells. UD slightly increased in type I collagen mRNA abundance throughout the culture period, whereas it markedly inhibited the gene expression of collagenase-1 between days 15 and 20 of culture. These results indicate that UD has anabolic effects on bone through the promotion of osteoblastic differentiation, suggesting that it could be used for the treatment of common metabolic bone diseases such as osteoporosis.

Anti-oxidant activity and effect of Pinus morrisonicola Hay. on the survival of leukemia cell line U937

The free radical scavenging and anti-cancer activites of Pinus morrisonicola Hay. were studied using different parts of the pine, namely, needle, bark and cone. Results showed that pine needle water extract has the highest scavenging superoxide anion activity and the lowest IC50 value in inhibiting superoxide anion formation; however, the bark water extract showed the best anti-lipid peroxidation activity. Additionally, needle water extract displayed the highest inhibition of leukemia cell line U937 growth. The results indicated that P. morrisonicola Hay. possesses potential chemopreventative and therapeutic properties.

Acute Toxicity of Water Extracts of Barks of Yellow Oleander, Thevetia peruviana (Persoon), and Neem Plant, Azadirachta indica (Lodd) to Mormyrids, Hyperopisus bebe occidentalis (Gunther)

Fingerling of mormyrids, Hyperopisus bebe occidentalis (Gunther), mean weight 13.02±0.54 g, were exposed in 45 × 25 × 20 cm3 glass aquaria to 0.00,0.05,0.25,0.50, and 1.00 mg/L concentrations of freeze-dried water extracts of barks of yellow oleander, Thevetia peruviana, and neem plant, Azadirachta indica, for 96 hours in a static bioassay test. The 96-hour LC50, opercular ventilation rates, and threshold values (maximum dose at which mortality does not occur) were determined. The 96-hour LC50 of the water extracts of bark of yellow oleander and the neem plant were 0.05 and 0.125 mg/L, respectively. Extracts of yellow oleander were observed to be more toxic than the extracts of the neem plant. Opercular ventilation increased markedly with exposure to both extracts, then declined as the fish became fatigued. The threshold value for the yellow oleander was observed to be lower, 0.047mg/L than that of the neem plant, 0.124 mg/L. Mortality is believed to be due to fatigue and direct toxic effects of the plant extracts on the fish tissues.

Anti-hyperglycemic Activity of Psidium guajava Bark Extract

Objective: To evaluate the anti-hyperglycemic activity of water extract of Psidium guajava stem bark. Materials and methods: The water extract of stem bark of Psidium guajava was tested for its hypoglycemic activity in normal glucose loaded and alloxan-induced hyperglycemic Wistar rats. Blood glucose levels were evaluated at intervals of 30 and 90 minutes in normal glucose loaded animals, at intervals of 0, 1 and 3 h in acute study and at days 1, 3, 7 and 10 during sub acute treatment after extract administration at an oral dose of 250 mg/kg. Results: During both acute and sub-acute tests, the water extract showed statistically significant and considerable anti-hyperglycemic activity and enhanced glucose tolerance in normal glucose loaded rats. Conclusion: Psidium guajava stem bark possessed statistically significant anti-hyperglycemic potential in alloxan induced diabetic rats and enhanced glucose tolerance in glucose loaded normal rats.