Bottled Water Research Articles

Molecular and serological diagnosis of multiple bacterial zoonoses in febrile outpatients in Garissa County, north-eastern Kenya

Bacterial zoonoses are diseases caused by bacterial pathogens that can be naturally transmitted between humans and vertebrate animals. They are important causes of non-malarial fevers in Kenya, yet their epidemiology remains unclear. We investigated brucellosis, Q-fever and leptospirosis in the venous blood of 216 malaria-negative febrile patients recruited in two health centres (98 from Ijara and 118 from Sangailu health centres) in Garissa County in north-eastern Kenya. We determined exposure to the three zoonoses using serological (Rose Bengal test for Brucella spp., ELISA for C. burnetti and microscopic agglutination test for Leptospira spp.) and real-time PCR testing and identified risk factors for exposure. We also used non-targeted metagenomic sequencing on nine selected patients to assess the presence of other possible bacterial causes of non-malarial fevers. Considerable PCR positivity was found for Brucella (19.4%, 95% confidence intervals [CI] 14.2–25.5) and Leptospira spp. (1.7%, 95% CI 0.4–4.9), and high endpoint titres were observed against leptospiral serovar Grippotyphosa from the serological testing. Patients aged 5–17 years old had 4.02 (95% CI 1.18–13.70, p-value = 0.03) and 2.42 (95% CI 1.09–5.34, p-value = 0.03) times higher odds of infection with Brucella spp. and Coxiella burnetii than those of ages 35–80. Additionally, patients who sourced water from dams/springs, and other sources (protected wells, boreholes, bottled water, and water pans) had 2.39 (95% CI 1.22–4.68, p-value = 0.01) and 2.24 (1.15–4.35, p-value = 0.02) times higher odds of exposure to C. burnetii than those who used unprotected wells. Streptococcus and Moraxella spp. were determined using metagenomic sequencing. Brucellosis, leptospirosis, Streptococcus and Moraxella infections are potentially important causes of non-malarial fevers in Garissa. This knowledge can guide routine diagnosis, thus helping lower the disease burden and ensure better health outcomes, especially in younger populations.

The poverty of sustainability: behavioral choices of drinking water in Kenya and Germany

Worldwide countries are engaged in technological improvements, changes in legislation, economic incentives, strengthened administrative structures, and political strategies to allocate and distribute water among different sections of the population. These macro measures treat water as an object of social, cultural, and environmental production unit for distribution and allocation, distancing it from the micro behavioral practices of drinking water. The poverty of sustainability stems from the gap between macro instrumental measures and the inner human dimensions that determine the micro practices. This paper explores the disconnect between macro sustainability measures and the micro practice of drinking water choice among individuals and their motives in the city of Nakuru in Kenya and the state of North Rhine-Westphalia in Germany. Both Kenya and Germany have developed top-down policies with a fix-it mindset. Kenya aims to provide drinking water to its population through piped network technology despite being a water-scarce region with inadequate housing and governance. In contrast, Germany, which is rich in water resources with highly regulated governance arrangements, spends billions treating water to attain drinking water quality only for most citizens to buy bottled water to drink. Despite water resources being finite, both of these nations source additional water through instrumental approaches to satisfy the demands of the materialistic world. In this context, the challenge of overcoming the poverty of sustainability remains a topic of debate that can be ameliorated by complementing scientific approaches with nonscientific knowledge and practices.

A Simple, Ecofriendly, and Fast Method for Nitrate Quantification in Bottled Water Using Visible Spectrophotometry.

There are many works associating the presence of nitrate in water and the occurrence of cancer in humans. The most common method for quantifying nitrate in water is based on the use of toxic cadmium as a reductant. In this work, a new approach was developed for the quantification of nitrate in bottled water with indirect spectrophotometry using Zn0 as a reductant. Nitrate is reduced to nitrite using Zn0 in a buffered medium (acetate/acetic acid) and quantified with visible spectrophotometry using the Griess reaction between sulfanilamide and N-(1-naphthyl)-ethylenediamine. The influence of pH, buffer solution (constitution and concentration), Zn0 (mass and granulometry), and agitation time on the efficiency of nitrite generation was evaluated. The optimal conditions were an acetate-acetic acid buffer solution with a concentration and pH of 0.75 mol L-1 and 6.00, respectively, and a Zn0 particle size of 20 MESH and Zn0 mass of 300 mg. The limits of detection and quantification (LoD and LoQ) were 0.024 and 0.08 mg L-1, respectively. The method's accuracy and precision were evaluated using the analysis of commercial bottled water. In conclusion, the use of Zn0 instead of cadmium provided a green method with excellent LoD/LoQ. Further, the method proved to be simple and easy to apply during outdoor analysis.

An unmodified recycled polyethylene terephthalate (PET) nanofibrous membrane for water-in-oil and oil-in-water emulsions separation

A nanofibrous membrane made of unmodified recycled polyethylene terephthalate (rPET) was fabricated by electrospinning using plastic water bottle as raw materials. Using rPET polymer to fabricate membranes may help limit the discharge of plastic bottle waste into the environment and can treat oily wastewater simultaneously, especially emulsions. The variation in the concentration of rPET polymer and the properties of electrospun nanofibers, such as surface morphology and fiber diameter, had a significant impact on both the efficiency of oil droplet capturing and the surface wettability of the membrane. Water-in-oil emulsions and oil-in-water emulsions were separated using the nanofibrous membranes of different concentrations of rPET polymer, and the separation efficiencies at different concentrations of oil, and oil types were measured. The rPET nanofibrous membrane exhibited a water contact angle of 140.1° and was superoleophilic in air and underwater. The membranes exhibited excellent separation performances: >98% separation efficiency and a flux of more than 6500 L m-2 h-1 for water in hexadecane and octane emulsions; >95 % efficiency and a flux of 1509.70 L m-2 h-1 after three separation cycles of water-in-oil emulsions. For oil-in-water emulsions, the separation efficiencies were higher than 95 %, and flux was more than 3525.22 L m-2 h-1 for hexadecane octane and heptane in water emulsions, with an efficiency of >87.9 % and flux of 4186.20 L m-2 h-1 after three separation cycles. Moreover, the as-prepared membrane exhibited high performance of oil-water separation in harsh conditions, including strong acidic, strong basic, and strong salt solutions, with separation efficiencies of more than 98 % and flux of more than 12,700 L m-2 h-1. However, as water accumulated on the membrane surface, absorption and separation efficiency and flux were lowered significantly. This suggests the need to enhance the efficiency of the membrane and reduce the chance of water accumulation on the membrane surface.

Heavy metals pollution, distribution and associated human health risks in groundwater and surface water: a case of Kampala and Mbarara districts, Uganda

Groundwater and surface water quality is of great significance for humanity as they serve as the primary drinking water sources globally. Due to population growth and the need to provide people with necessities that depend on water as an essential resource, these bodies of water are becoming more polluted. The present study involved the collection of groundwater and surface water samples from Kampala and Mbarara districts in Uganda during the dry and wet seasons. Also, concentrations of lead (Pb), manganese (Mn), cadmium (Cd), copper (Cu), and iron (Fe) were analyzed in order to evaluate the toxicity of metals, identify potential sources, and determine the health risk associated with their presence in water. Results showed that metals were observed with higher concentration during the wet season than the dry season. The concentrations of Fe (8.646 ± 0.00 mg/L), Mn (2.691 ± 0.01 mg/L) and Cd (0.090 ± 0.41 mg/L) measured in groundwater were significantly higher than those measured in surface water. However, only Cu was observed with higher concentration (0.322 ± 0.06 mg/L) in surface water during the wet season. Furthermore, the degree of contamination (Cd) and the heavy metal pollution index (HPI) were evaluated for both the wet and dry seasons. Results showed that few samples were found in the category of portable drinking water while majority are within the “poor” and “very poor” classes which require proper treatment before consumption. The oral hazard index (HIoral) results showed that none of the samples are suitable for consumption; therefore, cause potential non-carcinogenic health issues to the consumer (HIoral > 1). Moreover, children are more at risk than adults in the study districts (higher HI values in both seasons). This study recommends frequent monitoring of quality of water and also effluent waste treatment by the major source of pollution.

Preliminary Failure Analyses of Loaded Hot Water Bottles

Hot water bottles are widely utilised for their therapeutic advantages, such as relieving muscle tension and imparting warmth. However, the increasing frequency and potential risks associated with bursting or failure necessitate a detailed examination of the contributing factors as their failure is not fully understood in a scientific manner. With the apparent lack of analysis of hot water bottles in the literature, this study employs, for the first time, a dual methodology involving finite-element (FE) analysis conducted in ABAQUS and experimental validation to systematically investigate the underlying mechanisms leading to failure incidents. Through FE modelling and analysis, the stress and strain distribution within typical hot water bottles is modelled under compression loading conditions, facilitating the identification of vulnerable areas prone to failure. Experimental validation encompasses uniaxial loading compression tests on distinct specimens, generating load–displacement curves that elucidate material responses to compressive forces and highlight variations in load-bearing capacities. The study explores diverse failure modes, attributing them to stress concentration at geometric transitions and contact regions. Stress–strain curves contribute valuable insights into material characteristics, with ultimate stress values as crucial indicators of resistance to deformation and rupture. The FE analysis simulation results visualise deformation patterns and stress concentration zones. The findings illustrate that the highest stress concentration areas exist in the internal boundary of hot water bottles near the neck and cap region. This is experimentally confirmed through the bursting failures of four samples, with three failures occurring in this specific region. The findings support the guidance that users should avoid sleeping with a hot water bottle as it may fail under compression if they lay on top of it. Meanwhile, this result guides manufacturers to strengthen the weak areas of hot water bottles around the nicks and edges. This study significantly enhances our understanding of hot water bottle mechanics, thereby guiding design practice to improve overall performance and user safety. In summary, hot water bottles are commonly used but have not been investigated scientifically regarding external loading conditions and their related failure, as the current study has achieved. Identifying the weak points through experiment and simulation directs manufacturers towards required improvements in particular regions, such as the bottleneck and edge reinforcement during the design and manufacturing phases.

Effect of a Water, Sanitation, and Hygiene Program on Handwashing with a Cleansing Agent among Diarrhea Patients and Attendants in Healthcare Facilities in the Democratic Republic of the Congo: A Randomized Pilot of the PICHA7 Program.

Healthcare-acquired infections are a major problem in healthcare facility settings around the world. The Democratic Republic of the Congo (DRC) has over 2 million diarrhea patients hospitalized each year. These healthcare settings become high-risk environments for spreading diarrheal illnesses such as cholera. The objective of the Preventative Intervention for Cholera for 7 Days (PICHA7) program is to develop evidence-based water, sanitation, and hygiene (WASH) interventions to reduce cholera and other severe diarrheal diseases in the DRC. The study objective was to evaluate the effectiveness of PICHA7 program delivery in increasing handwashing with a cleansing agent at stool/vomit- and food-related events in a healthcare facility setting among diarrhea patients and patient attendants. A pilot of the PICHA7 program was conducted among 284 participants in 27 healthcare facilities from March 2020 to November 2021 in urban Bukavu in the South Kivu Province of the DRC. The standard arm received the standard message provided in the DRC to diarrhea patients on the use of oral rehydration solution and a basic WASH message at healthcare facility discharge. The PICHA7 arm received the PICHA7 WASH pictorial module delivered by a health promoter focused on handwashing with a cleansing agent at the bedside of the diarrhea patient in the healthcare facility and provision of a soapy water bottle (water and detergent powder). Within 24 h of intervention delivery, a three-hour structured observation of handwashing practices at stool/vomit- and food-related events (key events) was conducted in healthcare facilities of diarrhea patients and their attendants. Compared to the standard arm, there was significantly more handwashing with a cleansing agent at key events in the PICHA7 arm (40% vs. 15%) (odds ratio: 5.04; (95% confidence interval (CI): 2.01, 12.7)). These findings demonstrate that delivery of the PICHA7 WASH pictorial module and provision of a soapy water bottle to diarrhea patients and their attendants presents a promising approach to increase handwashing with a cleansing agent among this high-risk population in healthcare facilities in the eastern DRC.

Game Model to Improve the Motor Abilities of Primary School Students

This research aims to produce a learning model to improve children's motor skills through traditional games which are prepared based on the curriculum and characteristics of elementary school students. Research development procedures use Research and Development. The results of the development research obtained five game models, namely: bicycle wheel racing, rubber band throwing, rubber band unclang, mineral water bottle toss, and bicycle wheel rim target balls which were arranged in the form of a guidebook. The manual for motor skills game models for elementary school students was declared suitable for use based on the assessment of material experts and elementary school teachers. The results of the experts' assessments are as follows: 1) the bicycle wheel racing game is included in the "very good" category with an average rating of 4.8, which means X > 4.2; 2) the mineral water bottle gandon game is included in the "very good" category with an average rating of 4.9, which means X > 4.2; 3) the rubber band throwing game is included in the "very good" criteria with a mean rating of 4.86, which means X > 4.2; 4) the rubber band unclang game is included in the "very good" criteria with a mean rating of 4.9, which means X > 4.2; and 5) the bicycle wheel target ball game is included in the "very good" criteria with an average rating of 4.9, which means X > 4.2

Drinking water sources, quality, and associated health outcomes in Appalachian Virginia: A risk characterization study in two counties

ObjectivesIn the US, violations of drinking water regulations are highest in lower-income rural areas overall, and particularly in Central Appalachia. However, data on drinking water use, quality, and associated health outcomes in rural Appalachia are limited. We sought to assess public and private drinking water sources and associated risk factors for waterborne pathogen exposures for individuals living in rural regions of Appalachian Virginia. MethodsWe administered surveys and collected tap water, bottled water, and saliva samples in lower-income households in two adjacent rural counties in southwest Virginia (bordering Kentucky and Tennessee). Water samples were tested for pH, temperature, conductivity, total coliforms, E. coli, free chlorine, nitrate, fluoride, heavy metals, and specific pathogen targets. Saliva samples were analyzed for antibody responses to potentially waterborne infections. We also shared water analysis results with households. ResultsWe enrolled 33 households (83 individuals), 82% (n = 27) with utility-supplied water and 18% with private wells (n = 3) or springs (n = 3). 58% (n = 19) reported household incomes of <$20,000/year. Total coliforms were detected in water samples from 33% (n = 11) of homes, E. coli in 12%, all with wells or springs (n = 4), and Aeromonas, Campylobacter, and Enterobacter in 9%, all spring water (n = 3). Diarrhea was reported for 10% of individuals (n = 8), but was not associated with E. coli detection. 34% (n = 15) of saliva samples had detectable antibody responses for Cryptosporidium spp., C. jejuni, and Hepatitis E. After controlling for covariates and clustering, individuals in households with septic systems and straight pipes had significantly higher likelihoods of antibody detection (risk ratios = 3.28, 95%CI = 1.01–10.65). ConclusionsTo our knowledge, this is the first study to collect and analyze drinking water samples, saliva samples, and reported health outcome data from low-income households in Central Appalachia. Our findings indicate that utility-supplied water in this region was generally safe, and individuals in low-income households without utility-supplied water or sewerage have higher exposures to waterborne pathogens.

NATURE AND EFFECT OF WASTE GENERATED IN NASARAWA STATE, NIGERIA

The problem of indiscriminate waste disposal dates back to the earliest urban settlements in human society. The trend has been on the increase across many societies including Nigeria. This paper examined the nature and effect of waste generated in Nasarawa State, Nigeria. The study adopted the Reasoned Action Theory to explain the nature of waste generated in the study area. A cross-sectional survey design was adopted for the study. A sample of400 respondents was drawn through simple random, systematic sampling, clustered and purposive sampling techniques in the selection of Local Government Areas, electoral wards, towns/villages, main streets, houses, households and individuals. Primary data were generated through the use of questionnaire and in-depth interview. The questionnaire copies were analysed quantitatively using simple percentage while test frequency tables, mean and in-depth interview were unbundled using content analysis. The findings revealed that decomposed food items, empty sachet water packs and water bottles, empty engine oil gallons, used and unused drinking straw, sachets of all kinds, biscuit, condemned zincs and building materials were the solid wastes indiscriminately disposed in the study area. The study recommends that the Nasarawa State Waste Management Board should intensify the collection of solid wastes from the doorsteps of residents for appropriate recycling to mitigate the associated health and environmental hazards associated with the indiscriminate disposal of solid waste in the study area.

Detection, detoxification, and anti-counterfeiting applications of pullulan-modified nitrogen-doped carbon dots

Nitrogen-doped carbon dots (N-CDs) modified with pullulan were prepared using a hydrothermal method for use in sensing, detoxification, and anti-counterfeiting applications. The excitation-independent N-CDs demonstrated selectivity for hydroquinone and 4-nitrophenol (4-NP) with detection limits of 58 and 13 nM. The N-CD-based detection method was extended to environmental samples such as bottled water, tap water, and river water, and acceptable relative standard deviations (RSDs) and good recoveries were found. In the presence of NaBH4, N-CDs converted the toxic MB to colorless leuco-methylene blue in 10.5 minutes, confirming that the catalytic ability of N-CDs can be evaluated using MB as a model pollutant. With an excellent quantum yield of 31%, the N-CDs had the potential as a fluorescent ink for anti-counterfeiting applications by helping to visualize fingerprints, drawings, and chemical structures under 365 nm UV light while being completely invisible in daylight and sunlight. The N-CDs have significant potential for industrial use as fluorescent inks, detoxification catalysts, and pollutant sensors.

The origin and water quality of spring systems in Monchique, Portugal: A focus on Long-Term sustainability and elevated sodium levels

Spring water systems in Monchique, SW Portugal, not only serve diverse local utilities–from thermal baths to bottled water–but also represent a microcosm of a global concern: elevated sodium levels in spring-sourced bottled waters. This research employs hydrogeochemical and isotopic analyses to investigate the origin, hierarchy and quality of the springs offering a new focus on elevated sodium concentrations, a known global cardiometabolic risk factor. The springs arise from a complex hydrogeological system formed by extensive faulting between argillaceous country-rocks and intruded Cretaceous syenites. Here we show that thermal springs with temperatures of 23–31 °C, have a groundwater age of 5000 years, indicative of extended water–rock interactions and recharge at elevations of 450 to 650 m revealing a hitherto unreported resilience in regional groundwater hydraulics. Conversely, cold springs recharging from 200 m to the area’s peak at 900 m follow shorter paths within the regolith influenced by shallow geological features. The study detects nitrate contamination from surface sources in the cold springs and relatively high sodium levels in the geothermal springs that approach or surpass guideline values. Elevated sodium is attributed, for the first time, to long-term interactions and cation exchange with Na-rich syenites, possibly intensified by heat-induced dissolution at depth. This study informs current perspectives on the long-term sustainability and public health implications of spring water systems. The ion chemistry and isotopic evidence suggest that thermal springs, due to their longer circulation times, are less vulnerable to climatic shifts compared to the more susceptible cold springs. While offering specific insights into the Monchique springs, the research has broad implications to similar high-sodium thermal springs across the Iberian Peninsula and potentially worldwide.

Application of Game Theory in Solving Urban Water Challenges in Ibadan-North Local Government Area, Oyo State, Nigeria

The significance of water in human life cannot be ignored, but in most of the developing countries; availability, provision and access to good water source to the citizens has post major challenges. The nature of water scarcity has been majorly attributed to seasonal factor and high population pressure. This study therefore aimed at developing a practical approach and mechanism to solving water challenges in urban areas in Nigeria by means of game theory. The findings revealed that majority of respondents lack access to portable or drinkable water from water agency and that regular water supply sources have been traced to be borehole or well which was provided by citizens in the community at a cost. Applying the game theory approach, the outcome of the game indicates that the equilibrium value for the two players (the water agency and the residents) is N2,000 for 1,000 liters of water supply monthly. However, the payoff can be maiximised by every player (monetary values for the water agency and satisfactory water consumption for the residents). It is therefore importance that water agency should improve in the quality of their work in providing adequate water supply to the community to reduce or eradicate ailment arises from non availability of quality water.

The potential of chitosan intercalated nickel/iron layered double hydroxide composites as extraction adsorbent for phthalates

In this research, the potential utility of chitosan-nickel/iron LDHs (CS-Ni/Fe LDHs) has been examined for the separation and pre-concentration of five phthalates (PAEs: diethylphthalate (DEP), dipropylphthalate (DPP), dibutylphthalate (DBP), benzylbutylphthalate (BBP), and dioctylphthalate (DOP)). The extraction efficiency of CS-Ni/Fe LDH composites against the target phthalates is studied through the control of the key variables (e.g., eluent type, elution volume, flow rate, adsorbent amount, solution pH, and salt addition effect). Under the optimal conditions, the method detection limit towards target PAEs is estimated as 0.023–0.033 ng/mL over a 0.1–100 ng/mL range (R2>0.999). The CS-Ni/Fe LDH composites are utilized successfully to extract PAEs in real samples such as perfume, cream, bottled water, and bottled milk (recovery: 97.3–136.8%). The spent CS-Ni/Fe LDH composites can be regenerated at least over 30 cycles. The good agreement in adsorption isotherms with the non-linear Langmuir model indicates the formation of monolayered PAE molecules over CS-Ni/Fe LDH composites. The computed partition coefficients (mg g−1μM−1) for CS-Ni/Fe LDH are found as follows: DOP (802) > DBP (491) > DPP (355) > DEP (248) > BBP (247). The thermodynamic analysis reveals the exothermic and spontaneous behavior of the adsorption process. The CS-Ni/Fe LDHs are thus identified as efficient sorbent media for PAEs through hydrogen bonding and hydrophobic interactions. Accordingly, CS-Ni/Fe LDH composites are recommended as a useful option for capturing PAEs under real-world conditions.

Occurrence, migration and health risks of fluorescent whitening agents and phthalates in bottled water

The occurrence and health risks of fluorescent whitening agents (FWAs) in bottled water were reported for the first time. FWA184 and FWA393 were the most frequently detected FWAs, with mean concentrations of 3.99–17.00 ng L−1. Phthalates (PAEs) such as dibutyl phthalate (DBP), di-iso-butyl phthalate (DiBP), and diethylhexyl phthalate (DEHP) were prevalent in bottled water, with mean levels of 40.89–716.66 ng L−1, and their concentrations in bottled water were much higher than those of FWAs. FWAs and PAEs in bottles and caps were extracted using organic solvent, and the correlation analysis showed that FWA393 and DEHP most likely originated from bottles, while bottle caps were the main sources of DBP and DiBP. The calculated risk quotients (RQs) of target substances and all age groups were considerably lower than the threshold of 0.1, indicating that consuming bottled water containing these plastic additives was unlikely to pose health risks for people of all ages. However, RQ values for underage people were several times higher than those for adults and hence cannot be neglected; therefore, special attention should be paid to understand the potential risks posed by the exposure to these plastic additives during early life stages, especially the infant stage.

Engineering of Pt@Ni,N-doped graphene electrocatalyst based on recycled water bottles waste as an efficient cathode material for PEM fuel cells

Fuel cells (FCs) represent one of the most promising clean power sources. Their cost represents a considerable challenge to their widespread commercialization because of their sensitivity and the expensive cost of the employed platinum electrocatalysts. Accordingly, one feature of a fuel cell device that many interested parties are looking for is the engineering of catalysts with a low Pt content. Herewith, Pt supported on nickel loaded nitrogen-doped graphene (Pt@Ni,N-G) was synthesized through a simple pyrolysis process of mineral water bottles waste with urea and nickel metal at 800 °C followed by loading Pt in different proportions via a simple reduction method in aqueous solution at room temperature. The physicochemical and electrochemical properties of a promising Pt@Ni,N-G cathode electrocatalyst for polymer electrolyte membrane fuel cell (PEMFC) were characterized and evaluated using a number of specialized techniques. The results showed that the high Pt@Ni,N-G content enhanced the catalytic activity of the oxygen reduction reaction (ORR), with a specific activity of 3.13 mA cm−2 and a mass activity of 12.35 mA μg−1Pt at −0.1 V. A maximum power density of 87.8 mW cm−2 was achieved which is closest to that of commercial Pt/C. Designing Pt@Ni,N-G electrocatalysts by recycling waste bottles is not only expected to reduce the cost of fuel cells by ~30 % to raise the industry standard but also protect the environment and humanity from harmful pollutants.

Characterization of Thermotoga maritima Esterase Capable of Hydrolyzing Bis(2-hydroxyethyl) Terephthalate.

The gene-encoding carboxylesterase (TM1022) from the hyperthermophilic bacterium Thermotoga maritima (T. maritima) was cloned and expressed in Escherichia coli Top10 and BL21 (DE3). Recombinant TM1022 showed the best activity at pH 8.0 and 85 °C and retained 57% activity after 8 h cultivation at 90 °C. TM1022 exhibited good stability at pH 6.0-9.0, maintaining 53% activity after incubation at pH 10.0 and 37 °C for 6 h. The esterase TM1022 exhibited the optimum thermo-alkali stability and kcat/Km (598.57 ± 19.97 s-1mM-1) for pN-C4. TM1022 hydrolyzed poly(ethylene terephthalate) (PET) degradation intermediates, such as bis(2-hydroxyethyl) terephthalate (BHET) and mono(2-hydroxyethyl) terephthalate (MHET). The Km, kcat, and kcat/Km values for BHET were 0.82 ± 0.01 mM, 2.20 ± 0.02 s-1, and 2.67 ± 0.02 mM-1 s-1, respectively; those for MHET were 2.43 ± 0.07 mM, 0.04 ± 0.001 s-1, and 0.02 ± 0.001 mM-1 s-1, respectively. When purified TM1022 was added to the cutinase BhrPETase, hydrolysis of PET from drinking water bottle tops produced pure terephthalic acids (TPA) with 166% higher yield than those obtained after 72 h of incubation with BhrPETase alone as control. The above findings demonstrate that the esterase TM1022 from T. maritima has substantial potential for depolymerizing PET into monomers for reuse.

Physico-Chemical, Microbiological and Mineral Quality of Selected Boreholes and Stream Waters in Elele Community, Rivers State, Nigeria West Africa

The analysis of the quality of drinking water from selected boreholes in Elele Community of Rivers State was conducted. The results obtained were compared with WHO standards. The physicochemical parameters values obtained were as follows: colour (all clear), pH (4.09 – 7.04 with mean value of 5.11. which is slightly acidic), temperature (24.5 -32.0 with mean value of 29.2), total dissolved solids (5-180 mg/l with mean value of 46.6 mg/l), conductivity (8-242 μs/cm with mean value of 63μs/cm), chloride (&lt;1.0-24.7 mg/l), total hardness (&lt;0.2 mg/l), calcium (&lt;0.08), magnesium (&lt;0.05mg/l), total alkalinity (4.0 – 8.0mg/l with mean value of 5.0), sulphate (&lt;1.0mg/l), phosphate (&lt;0.05 mg/l), and nitrate (&lt;0.2 – 5.92mg/l), all of which did not exceed WHO standards, except for turbidity values in STN 7and 8 (5.51 and 7.40 NTU) which were slightly above the standard. For mineral analyses, Fe was in compliance with the standards, so also was Mn, except for STN 1 and 2 (2.576 and 0.471ppm) which were slightly above the limits. For microbiological analyses, FCB and TCB were less than detection limit, while THB values (2.4 x 102 – 8.0 x 102 efu/ml) all exceeded the WHO limits for portable water, which is the major source of contamination of the water samples. However, these variations in the results were not too pronounced to cause serious health hazards, since most of the parameters analyzed met the WHO limit and others which did not meet were slightly above the limit. But, this does not rule out completely the need for appropriate treatment of these water sources for portability and safe drinking. Recommendations on the strategies to reduce/eliminate some of the pollutants in the water were made.

Microplastics and non-natural cellulosic particles in Spanish bottled drinking water

This investigation explored the presence of microplastics (MPs) and artificial cellulosic particles (ACPs) in commercial water marketed in single use 1.5 L poly(ethylene terephthalate) bottles. In this work we determined a mass concentration of 1.61 (1.10–2.88) µg/L and 1.04 (0.43–1.82) µg/L for MPs and ACPs respectively in five top-selling brands from the Spanish bottled water market. Most MPs consisted of white and transparent polyester and polyethylene particles, while most ACPs were cellulosic fibers likely originating from textiles. The median size of MPs and ACPs was 93 µm (interquartile range 76–130 µm) and 77 µm (interquartile range 60–96 µm), respectively. Particle mass size distributions were fitted to a logistic function, enabling comparisons with other studies. The estimated daily intake of MPs due to the consumption of bottled water falls within the 4–18 ng kg−1 day−1 range, meaning that exposure to plastics through bottled water probably represents a negligible risk to human health. However, it's worth noting that the concentration of plastic found was much higher than that recorded for tap water, which supports the argument in favour of municipal drinking water.

The first investigation on the radiotoxicity risks associated with ingesting radium in drinking water from the Oujda Region-Morocco and some bottled mineral waters

Drinking water is essential to human life. However, it can be polluted by various factors, including radioactive substances such as radon 222Rn and radium 226Ra. Therefore, the determination of their concentrations is important for public health. The aim of this work is to measure the concentration of 226Ra in samples of tap, natural spring and well water taken from different sources in the eastern region of Morocco, as well as in a few samples of bottled mineral water. We used an AlphaGUARD detector with an AlphaKit accessory and an RTM1688-2 to carry out measurements of radon in secular equilibrium with radium. The got results show that the 226Ra activity is less than 0.104 ± 0.023 Bq/L, the Annual Effective Dose (AED)) for adults and children is less than 29.1 ± 4.7 μSv.y−1and 123.8 ± 4.7 μSv.y−1 for infants. The chemical toxicity risk evaluated using the Lifetime Average Daily Dose (LADD) was found less than 0.23 ± 0.05 μgkg−1day−1.The obtained results are reasonable in relation to international guidelines, and do not present any radiological hazard to consumers that could be attributed to the radium and radon in the analyzed water samples.