Water Balance Research Articles

EGFR inhibition with erlotinib induces membrane accumulation of aquaporin-2 via PDK1 and RSK activation

In the canonical aquaporin-2 (AQP2) signaling pathway, vasopressin (VP) binds to its V2 receptor, which in turn activates adenylate cyclase and protein kinase A (PKA), resulting in AQP2 S256 phosphorylation and accumulation of AQP2 in the membrane. The epidermal growth factor receptor (EGFR) inhibitor erlotinib also increases AQP2 S256 phosphorylation and membrane accumulation, yet it does not increase cAMP or PKA activity. We hypothesize that the ribosomal s6 kinase (RSK), a downstream effector in the EGFR-MAPK/ERK pathway, is the terminal kinase phosphorylating AQP2 in this novel PKA-independent pathway activated by erlotinib, and that the phosphoinositide dependent kinase-1 (PDK1), a well-established activator of RSK, is indispensable for erlotinib-induced AQP2 activation. Using our LLC-AQP2 cell model, we show that erlotinib-induced AQP2 membrane accumulation and S256 phosphorylation are abolished by the specific RSK inhibitor BI-D1870. RSK knockdown with siRNA also blocked AQP2 S256 phosphorylation and membrane accumulation, as did RSK knockout with CRISPR/cas9 editing. Next, rat kidney slices were incubated in Hank’s buffer with or without BI-D1870 and treated with erlotinib. BI-D1870 inhibited AQP2 membrane accumulation in collecting duct principal cells, supporting the relevance of this pathway in kidney cells in situ. An in-vitro kinase assay using purified proteins demonstrated that RSK directly phosphorylates AQP2 at the S256 residue. Additionally, erlotinib consistently increased phosphorylation of RSK T359, a residue associated with RSK’s active state. In canonical RSK activation, a phosphorylation cascade results in PDK1 binding to and activating RSK. We found that PDK1 inhibition with an experimental compound PS-222 also blocked erlotinib-induced AQP2 membrane accumulation and S256 phosphorylation. This implicates PDK1 along with RSK in the novel erlotinib-induced pathway of AQP2 traffcking. Our data show that RSK and PDK1 are terminal effectors in the novel, PKA-independent pathway of AQP2 activation induced by erlotinib. Further elaboration of this new non-canonical pathway promises to uncover potential pharmacological targets for the treatment of water balance disorders. This work was supported by the National Institutes of Health (NIH) grant DK096586 (D. Brown). P. W. Cheung was supported was supported by NIH K-award DK115901. Richard Babicz is the recipient of the Ben J Lipps Research Fellowship (American Society of Nephrology). Additional support for the Program in Membrane Biology Microscopy Core came from the Boston Area Diabetes and Endocrinology Research Center (DK057521) and the Massachusetts General Hospital (MGH) Center for the Study of Inflammatory Bowel Disease (DK043351). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Insights to the water balance of a Boreal watershed using a SWAT model

The hydrological characteristics of a watershed play a crucial role in shaping ecosystems within the Boreal zone and have a significant impact on regional environments. Knowing these characteristics, such as the distinctive topography, vegetation, soil composition, and climatic conditions in the Canadian Boreal ecozone, is essential for implementing sustainable water management. This study focuses on assessing the hydrological dynamics of the Upper Humber River Watershed (UHRW) in western Newfoundland, Canada, using the Soil and Water Assessment Tool (SWAT) model. The UHRW includes sub-basins and hydrological response units (HRUs), with diverse land uses, soil types, and slope characteristics. Key parameters influencing streamflow simulation were identified through sensitivity analysis, including the runoff curve number, the effective hydraulic conductivity, the temperature lapse rate, the soil evaporation compensation factor, and the available water capacity of the soil layer. The SWAT model, using data from the Reidville hydrometric station, shows favorable performance metrics, with R2 values of 0.79 and 0.83 during the calibration and evaluation periods, respectively. The model effectively captures seasonal and monthly flow patterns, displaying right-skewed distributions and seasonal variations. The analyzed hydrological parameters, such as precipitation, evaporation, transpiration, surface runoff, and groundwater flow, reveal their significant contributions to the water balance. The flow duration curve analysis indicates the model’s capability to estimate peak and low flows, with slight under-prediction during the recession phase. Seasonal analysis further supports the model’s performance, with positive Nash-Sutcliffe Efficiency (NSE) values ranging from 0.65 to 0.91. The study concludes that the SWAT model is suitable for simulating the hydrological processes in the studied watershed providing valuable insights for sustainable water resource management and decision-making in the UHRW. The results can be useful for other Boreal ecozone watersheds.

Groundwater recharge estimation in Mediterranean mountain environments by isotope profiles – Partitioning of macropore and matrix flow

Developing and continuously improving effective and feasible methods to estimate groundwater recharge is essential to ensure sustainable resource assessment. Mountain environments represent key areas for the regional water balance. In this study, the isotope profile method was used to investigate water fluxes through the vadose zone and subsequent groundwater recharge in a Mediterranean mountain environment. Six high-resolution soil water isotope profiles were taken at two different locations in the Troodos Massif (Cyprus) in the eastern Mediterranean, focusing on estimating recharge in the soil of hillslopes. Analysis of the isotope profiles yielded recharge rates ranging from 30–70 mm/year, leading to a re-evaluation of previous studies on groundwater recharge. The comparison of results obtained by the peak-shift method and mathematical modeling was used to partition matrix and macropore flow, reaching up to 39 %. Potential methodological limits of the environmental isotope profile method in skeleton-rich soils were found.

Exploring the hydrologic regulation and pollutant release of complex paddy systems in a large-scale agricultural catchment

Complex paddy systems (CPSs) are prevalent in humid agricultural regions, presenting a dilemma between restoring them to wetlands or maintaining them for food production. Thus, a comprehensive assessment of CPSs is necessary. However, the current model’s description of CPS is insufficient, leading to incomplete representation of the water and pollutant transfer processes of CPS in the model. Therefore, a new version of the soil and water assessment tool (SWAT-CPS) was proposed, which utilizes physically based rules to measure the horizontal seepage-overflow process within CPSs. Overall, the SWAT-CPS outperforms SWAT in streamflow, water cycle simulation, hydrological event reproduction and pollutant release simulation. Water balance analysis and scenario simulations showed that CPSs increase baseflow and pollutant release throughout the year (about 7.69% and 3.05%, respectively) while affecting quickflow in different ways depending on the streamflow conditions. Changing the area of the CPS may have significant impacts on the hydrological composition. This study highlights the differences between CPSs and wetlands in hydrological regulation and pollutant release. In fact, not all areas are suitable for “returning farmland to wetlands”. Our work providing insights for decision-makers in planning the allocation of CPS and wetland shares.

Calibration and validation of DRAINMOD to predict long-term permeable pavement hydrology

The hydrologic performance of permeable pavement varies widely due to underlying soil type, drainage configuration, contributing drainage area, surface infiltration rate, and aggregate depth. A long-term hydrologic model is needed to better understand the influence of these design variables on surface runoff, drainage, exfiltration, and evaporation from permeable pavement systems. Most permeable pavement models have not been calibrated with field monitored data, are usually unable to accurately model internal water storage (IWS) zones, and do not account for evaporation from the aggregate profile. Because permeable pavement employs drainage and exfiltration as primary hydrologic mechanisms, it was hypothesized that DRAINMOD, a model shown to accurately simulate bioretention hydrology, could be calibrated to predict the hydrologic response from permeable pavements. Hydrologic data were collected from two permeable pavement applications in North Carolina (Boone and Durham) and two permeable pavement applications in Ohio (Perkins Township and Willoughby Hills) to calibrate and test the model. The specific permeable pavements applications varied widely in aggregate depth, drainage configuration, underlying soil type, and contributing drainage area. Nash-Sutcliffe Efficiencies for drainage ranged from 0.77 to 0.95 during calibration and validation of all sites. Nash-Sutcliffe Efficiencies for exfiltration/evaporation ranged from 0.55 to 0.97 for Boone and Perkins Township, but prediction of exfiltration/evaporation volumes on an event basis was poor for the remaining two sites due to low exfiltration rates (less than 0.20 mm/hr). Despite this, the cumulative volume of exfiltration/evaporation was predicted to within 1–9 % of what was measured during monitoring. Modeled and monitored surface runoff from the Willoughby Hills application (8 % of the water balance) was equivalent. The model predicted the percentage of drainage, surface runoff, and exfiltration/evaporation to within 2 % of what was monitored/estimated at each site, suggesting long-term modeling of permeable pavement hydrology with DRAINMOD is viable.

Generation of an Inducible Avp Knockout Mouse Line Using CRISPR/Cas9 and Cre/LoxP

Background: Arginine vasopressin (AVP) is a peptide hormone coded by the Avp gene, synthesized in the hypothalamus and secreted by the posterior pituitary. Dysregulation of AVP secretion contributes to a variety of human diseases. Previous studies of functional roles of AVP have been largely dependent on the use of Brattleboro rats, which manifest a spontaneous mutation in the Avp gene and lack circulating AVP. Despite their utility, Brattleboro rats are diffcult to breed owing largely to the fixed nature of the Avp mutation, resulting in increased neonatal death and behavioral effects in the adults. Consequently, commercial breeders have ceased production, despite a continued need. To fill this need, we have now created a mouse model with a tamoxifen-inducible deletion of AVP. Methods: We employed CRISPR/Cas9 to target the desired regions (first intron and 3' downstream) within the Avp gene. Successful insertion of the two loxP sites was confirmed by PCR using primers flanking the targeted regions. Restriction enzymes BamHI or EcoRI were used to confirm correct targeting. For additional confirmation, PCR products were cloned into the TA-cloning vector and sequenced. Mice harboring the floxed allele were mated to B6.Cg-Tg(CAG-cre/Esr1*)5Amc/J mice that globally express a tamoxifen-inducible Cre recombinase. Results: The resultant homozygous conditional knockout mice ( Cre+ Avpflx/ flx) are viable, fertile, and show no signs of polydipsia or polyuria prior to induction, indicating that the conditional alleles retain their wild-type function. Induction of Cre-mediated recombination by administration of tamoxifen (2-3 mg q.d., i.p. for 5 days) in 8-week-old mice resulted in a decrease in urine osmolality from 2076±138 to 122±6 mOsm/kgH2O on day 31 after induction (p<0.0001). Sanger sequencing demonstrated the expected 1245 bp deletion at the Avp locus. The kidney parenchyma was structurally normal with no evidence of medullary atrophy 6 weeks after induction. Immunoblotting of AQP2 in the inner medulla showed a significant decrease in AQP2 band density in Cre+ Avpflx/ flx mice to 27±14 % of values in Cre− floxed control mice (p<0.05). Conclusion: We developed an inducible AVP knockout mouse line ( Cre+ Avpflx/ flx) that will be shared with the research community and are likely to be useful for further study of regulation of water balance and polycystic kidney disease, as well as neural, vascular, and metabolic roles of vasopressin. The work was funded by the Division of Intramural Research, National Heart, Lung and Blood Institute. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Water balance estimation for an ordinary solid waste sanitary landfill in Costa Rica

To assess the necessity of treatment systems for leachate generated by ordinary solid waste deposited in a sanitary fill, accurate estimation of the leachate and gas amount produced during biodegradation process is essential. Climatic conditions at the site significantly influence leachate generation; therefore, accurate estimation of rainfall, temperature, solar radiation, and infiltration into the fill is imperative. In this study, we analyzed the water balance of the Miramar landfill in Costa Rica, assessed the amount of leachate produced using various methodologies, and then compared these findings with the field data to identify the best methodology for specific environmental conditions. To comprehensively analyze landfill behavior, as well as the leachate and gases generated from waste decomposition, determining the hydraulic properties of the materials inside the cells is necessary. These properties must consider variables such as the types of materials present at the site, age of the ordinary waste, and their densities. The hydraulic properties of the materials were obtained from various laboratory tests, which included determining the soil–water characteristic curve necessary for assessing unsaturated conditions in the solid waste. These properties are compared with the results of studies conducted in other landfills to validate the current methodology and the obtained results, which can be used for conducting studies on leachate generation or slope stability analysis for sanitary landfill slopes. The amount of leachate generated was estimated using the methodology proposed by Delgado A. (2018). Additionally, the Hydrologic Evaluation of Landfill Performance model (HELP V 4.0) was employed, demonstrating superior estimation compared to recorded field data. The HELP V 4.0 model facilitated the incorporation of different material layers and thicknesses, weather information at the site, slopes, and drainage conditions.

Model calibration using hydropedological insights to improve the simulation of internal hydrological processes using SWAT+

AbstractSoils affect the distribution of hydrological processes by partitioning precipitation into different components of the water balance. Therefore, understanding soil‐water dynamics at a catchment scale remains imperative to future water resource management. In this study, the value of hydropedological insights was examined to calibrate a processes‐based model. Soil morphology was used as soft data to assist in the calibration of the Soil Water Assessment Tool (SWAT+) model at five different catchment scales (48, 56, 174, 674, and 2421 km2) in the Sabie River catchment, South Africa. The aim of this study was to calibrate the SWAT+ model to accurately simulate long‐term monthly streamflow predictions as well as to reflect internal soil hydrological processes using a procedure focusing on hydropedology as a calibration tool in a multigauge system. Results indicated that calibration improved streamflow predictions where R2 improved by 2%–8%. Nash‐Sutcliffe Efficiency (NSE) improved from negative correlations to values exceeding 0.5 at four of the five catchment scales compared to the uncalibrated model. Results confirm that soil mapping units can be calibrated individually within SWAT+ to improve the representation of hydrological processes. Particularly, the spatial linkage between hydropedology and hydrological processes, which is captured within the soil map of the catchment, can be adequately reflected within the model simulations after calibration. This research will lead to an improved understanding of hydropedology as soft data to improve hydrological modelling accuracy.

Research on land subsidence-rebound affected by dualistic water cycle driven by climate change and human activities in Dezhou City, China

Over-exploitation of groundwater is the principal anthropogenic trigger for land subsidence in the North China Plain (NCP). With the promulgation of policies on groundwater extraction restrictions and bans, seasonal fluctuations and uplift have begun to occur in some typical subsidence areas in the NCP. In order to explore the land subsidence-rebound drivers, this study takes Dezhou City, a typical subsidence area in the NCP, as the study area and selects the main drivers in climate change: precipitation and evapotranspiration and the main driver in human activities: groundwater extraction, which together forms the background of the regional dualistic water cycle. In this background, this study selects the corresponding datasets and uses the improved Water Balance constraints Recurrent Neural Network (WB-RNN) method to establish the link between precipitation data, evapotranspiration data, groundwater extraction and groundwater level. Then uses the Gate Recurrent Unit-Convolutional Neural Network (GRU-CNN) method to establish the link between groundwater level and Interferometric Synthetic Aperture Radar (InSAR) deformation, and then obtains the link between the drivers of the dualistic water cycle and land subsidence. Comparative analysis and changing model inputs found that 1) Seasonal fluctuations in land subsidence-rebound in Dezhou City are mainly controlled by the groundwater levels in Aquifer Groups III and IV, with amplitudes up to 15 mm per year. 2) Groundwater level changes in different layers lag precipitation at different times. Excluding the influence of human extraction factors, the maximum ground surface deformation fluctuation caused by monthly 260 mm precipitation can be up to 21.44 mm. 3) The impact of short-term heavy precipitation caused by Typhoon Lekima and Doksuri in 2019 and 2023 can cause deformation fluctuations to a certain extent.

Areal artificial recharge has changed the interactions between surface water and groundwater

Artificial groundwater recharge is increasingly utilized globally for addressing regional groundwater issues due to its effectiveness and ease of application. However, due to the complexity of the interaction of groundwater systems with the external environment and its inherent latency, governance effects are difficult to quantify and are not fully understood yet. In this study, the efficiency of areal artificial groundwater recharge was determined and the interactions with surface water were analyzed by field surveys, sample analysis, water balance reconstruction, and model simulation. A typical groundwater recharge region in the Beijing-Tianjin-Hebei region, China, was focused. Additionally, the response of groundwater flow and water quality to areal artificial groundwater recharge was quantified. Results showed that areal artificial groundwater recharge changes the relationship between surface water and groundwater, with an efficiency of 70 % under high recharge and fluctuation, and decreasing to 20–30 % after the groundwater level was stabilized. The impact on the hydrodynamic field were primarily manifested as the formation of water mounds near river channels, which expand laterally up to 9.2 km from the recharging river channel one year after implementation.Then the impact increases to 14.9 km and continues to expend a year after cessation. The areal artificial groundwater recharge has simultaneously improved the water quality by 23 % in monitoring wells within a 10 km distance from the river channel. The results of this study quantify the impacts of area artificial recharge on groundwater and provide references for the improvement of water resources and water environment in those similar regions.

Focal adhesion kinase (FAK) inhibition induces aquaporin 2 (AQP2) plasma membrane accumulation in renal epithelial cells by remodeling the actin cytoskeleton

The actin cytoskeleton is involved in the cellular traffcking of the AQP2 water channel in kidney collecting duct (CD) principal cells (PC): this plays an important role in water homeostasis. Focal adhesion kinase (FAK) is known to modulate the actin cytoskeleton through small GTPase-mediated pathways and we, therefore, investigated the potential role of FAK in AQP2 traffcking. We and others have previously shown that AQP2 constitutively traffcs and recycles independently of vasopressin (VP) and that this involves actin depolymerization/polymerization; therefore, FAK could regulate AQP2 traffcking through actin remodeling, bypassing the VP-V2R (cAMP/PKA) signaling pathway. To examine this, we used small molecule FAK inhibitors, VS-4718, GSK2256098 (GSK), or Defactinib-Hydrochloride (DH). We used immunofluorescence staining, a transferrin endocytosis assay ± cold block treatment, a fluorometric exocytosis assay using cells expressing secreted yellow fluorescent protein (ssYFP), a RhoA activity assay, and western blotting. Inhibiting FAK with 1mM VS-4718 for 30 min increased AQP2 membrane accumulation in LLCPK1 and IMCD kidney epithelial cells expressing AQP2. VS-4718 resulted in reduced clathrin-mediated endocytosis, as well as increased vesicle exocytosis, both mechanisms contributing to increased AQP2 membrane accumulation. This effect of FAK inhibition was independent of vasopressin/cAMP signaling and did not require phosphorylation of the Serine 256 residue of AQP2, since AQP2 membrane accumulation also occurred in cells expressing the constitutive dephosphorylation AQP2 S256A mutant. The FAK inhibitor VS-4718 promoted actin depolymerization (phalloidin staining assay) which would facilitate AQP2 vesicle exocytosis into the plasma membrane. We then examined the activity of the small GTPase, RhoA, whose activity increases actin polymerization and vesicle traffcking in the exocytic and endocytic pathways. We found that FAK inhibition by VS-4718 downregulated RhoA activity using GST-RBD, a substrate that binds to active RhoA, as seen by western blotting using phospho-specific antibodies. Similar results were obtained with GSK and DH. In summary, our study revealed that the interaction between FAK and cytoskeletal architecture plays a crucial role in regulating AQP2 recycling and its subsequent entry into the endocytic pathway. Thus, targeting FAK could be a potential therapeutic strategy for water balance disorders involving defective AQP2 reabsorption, such as nephrogenic diabetes insipidus. This work was supported by NIDDK R01DK096015 (JHL) and DK096586 (DB). This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Impacts of extreme precipitation on water conservation in Beijiang River Basin, China

Water conservation, which has been regarded as one of the most crucial ecosystem service functions of river basins, is a key factor for sustainable development, that has garnered widespread attention in the context of climate change. However, little attention has been paid to daily water conservation and its response to extreme precipitation events. In this study, we selected the Beijiang River Basin (BJRB) as the study area, which is an important ecological barrier for the Guangdong-Hong Kong-Macau Greater Bay Area (GBA), based on the distributed hydrological model WEP-L (Water and Energy transfer Processes in Large River basin) and water balance equation, the spatiotemporal characteristics of extreme precipitation and four daily water conservation indicators (maximum 1 day, maximum 3 day, maximum 5 day, maximum 7 day water conservation) in the BJRB from 1965 to 2009 were analysed, and the responses of these indicators to extreme precipitation were investigated. The results reveal that (1) the amount (RX1day, RX3day, RX5day, RX7day, R95p, and R99p), intensity (SDII), and frequency (R50mm) of extreme precipitation increased in the BJRB during 1965–2009, with SDII undergoing a significant increase. (2) The four water conservation indicators were positive, indicating that the watershed reduced peak flow during the extreme precipitation period by accumulating precipitation. The rate of increase of the four water conservation indicators were lower than that of extreme precipitation indices, there will be more extreme precipitation converted into surface runoff, thereby increasing the risk of flooding. The four indicators increased in the southern and northern regions of the BJRB, while decreased in the central region during 1965–2009. (3) The four indicators were generally positively correlated with most of the extreme precipitation indices in the northern BJRB, especially RX1day, RX3day, RX5day, RX7day, R99p, and R50mm. The results provide a scientific reference for water conservation adaptation to extreme precipitation variations, protection of the ecological environment, and the prevention and reduction of disasters in the region.

Quantifying Meteorological Drought Severity for the Watersheds in Euphrates River Basin, Central Euphrates District, Iraq

Drought is a major natural extreme event that can cause significant damage to water resources. This study investigated the implications of drought for Watersheds in Euphrates River basin in Iraq which includes Hilla, Karbala, Najaf, Diwaniyah, and Najaf.. It was found that had an adverse impact on the study region. Among the most often used drought assessment indices globally are the Standard Precipitation Index (SPI) and its version, the Standard Precipitation and Evapotranspiration Index (SPEI).The study evaluated several probability distribution functions to model rainfall and water balance in Iraq. The Kolmogorov–Smirnov (K–S) test and the Anderson–Darling (A–D) test were used to test the goodness-of-fit for rainfall and water balance. The log-normal distribution was found to be the best fit for rainfall at approximately 40% of the stations considered, while the generalized logistic distribution (genlog) was the best fit for water balance at approximately 80% of the stations considered. The study also found that the extreme droughts in Samwah in 2012 and Diwiniyah in 1999 had the highest severity values of -2.8957 in SPI-12 and SPEI-12, respectively.

Use of Soil Moisture as an Indicator of Climate Change in the SUPer System

Soil moisture can be an important indicator of climate change in humid and semi-arid areas. This indicator can more efficiently propose different public policies related to climate change than just using precipitation and temperature data. Given the above, the objective of this study is to evaluate changes in soil moisture in the state of Pernambuco during the period 1961–2021, using the System of Hydrological Response Units for Pernambuco. In this study, two river basins in the state of Pernambuco that represent the different climatic conditions of the state were chosen. The results show that in the coastal region there is a tendency towards more saturated soils, and in the semi-arid region there is a tendency towards drier soils. With these results, it is possible to conclude that public policy decisions for the economy, environment, and society must consider this vital water balance variable. Leveraging soil moisture and precipitation data makes it possible to differentiate between flood risks and landslide vulnerabilities, particularly in regions characterized by higher levels of rainfall. Monitoring soil water content in humid and semi-arid areas can significantly enhance early warning systems, thereby preventing loss of life and minimizing the socio-economic impacts of such natural events. As such, this study provides a holistic understanding of the relationship between climatic patterns, soil moisture dynamics, and the occurrence of droughts and floods, ultimately contributing to more effective disaster preparedness and response measures in Pernambuco and similar regions.

Identification of core genes involved in the response of Apocynum venetum to salt stress based on transcriptome sequencing and WGCNA.

Apocynum venetum L. belongs to the Apocynaceae family and is a plant that is highly resistant to stress. It is important in the fields of ecology, feeding, industry and medicine. The molecular mechanism underlying salt tolerance has not been elucidated. In this study, RNA-seq based transcriptome sequencing of A. venetum leaves after 0, 2, 6, 12, 24 and 48 h of treatment with 300 mM NaCl was performed. We conducted a comprehensive analysis of the transcriptome expression profiles of A. venetum under salt stress using the WGCNA method and identified red, black, and brown as the core modules regulating the salt tolerance of A. venetum. A co-expression regulatory network was constructed to identify the core genes in the module according to the correlations between genes. The genes TRINITY_DN102_c0_g1 (serine carboxypeptidase), TRINITY_DN3073_c0_g1 (SOS signaling pathway) and TRINITY_DN6732_c0_g1 (heat shock transcription factor) in the red module were determined to be the core genes. Two core genes in the black module, TRINITY_DN9926_c0_g1 and TRINITY_DN7962_c0_g1, are pioneer candidate salt tolerance-associated genes in A. venetum. The genes in the brown module were mainly enriched in two pathways, namely photosynthesis and osmotic balance. Among them, the TRINITY_DN6321_c0_g2 and TRINITY_DN244_c0_g1 genes encode aquaporin, which is helpful for maintaining the cell water balance and plays a protective role in defending A. venetum under abiotic stress. Our findings contribute to the identification of core genes involved in the response of A. venetum to salt stress.

Improving mean water lake surface elevation estimates using dense lidar measurements from the GEDI satellite mission

Lakes are an essential component of the global water cycle and are considered as sentinels of climate change. An accurate estimate of lake's water levels is necessary for reliable continuous monitoring of their water balance. Due to their huge number worldwide, satellite altimetry has been identified as an efficient tool for measuring their temporal variations of water level. This technique can reach an accuracy of a few centimeters for water level estimates over large lakes. Due to the coarse across-track spatial resolution of the radar altimetry missions, their spatial sampling only covers a small part of the lakes surface. To obtain an accurate estimate of the lake water level, it is necessary to apply a correction to account for the small-scale orthometric height undulations occurring on the lake area and not present in the current global geoid models. In this study, the high-density lidar measurements from GEDI are used to estimate the mean lake surface. From the 1,366,211 GEDI data over 1239 tracks acquired over more than 6000 km2 of the surface of Lake Issy-Kul (Kyrgyzstan, Central Asia) between May 2019 and mid-November 2021, 654,592 shots over 843 tracks were selected after a multi-step quality-check and processed to generate a mean lake surface of Lake Issyk-Kul. Comparison between the GEDI-based mean lake surface and GNSS kinematic surveys corrected from the lake level variations exhibit an RMSE of 0.065 m. The principal objective of performing such mean lake surface is to further use it in the framework of the calibration/validation of the Surface Water and Ocean Topography (SWOT) mission.

Sustainability in Aquatic Ecosystem Restoration: Combining Classical and Remote Sensing Methods for Effective Water Quality Management

The utilization of Effective Microorganisms (EMs) for lake restoration represents a sustainable approach to enhancing water quality and rebalancing the ecology of aquatic ecosystems. The primary objective of this study was to evaluate the effects of two bioremediation treatment cycles employing EM-enriched biopreparations on water quality in the Siemiatycze lakes. Specifically, this research analyzed various parameters, including dissolved oxygen, transparency, chlorophyll-a, pH, chemical oxygen demand (COD), biochemical oxygen demand (BOD5), total phosphorus, total nitrogen, and suspended matter (SM), across eleven designated sampling locations. Additionally, this study employed remote sensing techniques, leveraging Sentinel-2 satellite imagery and the Maximum Chlorophyll Index (MCI), to detect and quantify algal blooms, with a particular focus on elevated chlorophyll-a concentrations. This comprehensive approach aimed to provide a holistic understanding of the impact of biotechnological reclamation on aquatic ecosystem restoration and sustainability. The study’s findings indicated a significant improvement in water quality in all lakes, with enhanced water clarity and oxygen profiles. Further, remote sensing studies indicated a reduction in algal blooms, particularly those with high chlorophyll-a concentrations. A considerable decrease in water eutrophication intensity was observed due to diminished nutrient concentrations. The improvements in water parameters are likely to enhance the living conditions of aquatic organisms. These results demonstrate the effectiveness of using EM-enriched biopreparations in the bioremediation of lakes, providing a sustainable approach to enhancing water quality and balancing aquatic ecosystems.

Assessing the Performance of Conveyance System of Jato Small-Scale Irrigation Scheme of Wayu-Tuka District, East Wallaga Zone, Oromia, Ethiopa

The performance assessment of a small-scale irrigation project plays a vital role in evaluating its effectiveness and addressing potential shortcomings in achieving desired outcomes. This study aims to provide a comprehensive analysis of the Jato small-scale irrigation project, assessing its performance based on various criteria and indicators. The study evaluates the project's infrastructure maintenance and system efficiency. The designed command area of the scheme is seventy-five (75 ha) but the current irrigated area of the scheme is sixty (60ha) only. To achieve the objectives, primary and secondary data were collected. To gather both primary and secondary data, data-gathering techniques include surveys, interviews, field observations, and meteorological data. Before, during, and after an irrigation event, while farmers were carrying out their regular irrigation practices, several field characteristics were measured and/or observed. These data included the type of crop, irrigation water discharge in the canal, and field size. A survey concerning the availability of water was also carried out among farmers. CROPWAT 8.0 Software was used to organize the crop pattern of the study area along with other relevant data, including soil, climate, rain crop, and crop pattern. Water flow measurement results of the Jato small-scale irrigation scheme at the head, middle, and tail of the lined & unlined main canal were found to be 53.6l/s, 45.4 l/s, and 29.9l/s respectively. The average water flow measurement result of the scheme is 43l/s which reduced from required flow by 18.15l/s. From the analyses of the water balance indicators, the canal conveyance efficiency, canal conveyance loss, application efficiency, and overall efficiency were found to be 70%, 30%, 70%, and 50% respectively. The overall average main canal conveyance efficiency and water conveyance loss were below the recommended values. The studies have shown that the effective infrastructure, irrigation ratio, and water surface elevation ratio of the scheme were found to be 66.7%, 80%, and 66% respectively. However, there were certain areas for improvement identified, such as the need for better coordination among project stakeholders and enhanced maintenance practices to ensure the long-term functionality of irrigation infrastructure.

Influence of landscape pattern changes on water conservation capacity: A case study in an arid/semiarid region of China

With the accelerating changes of land use/cover caused by natural and human factors, landscape pattern has undergone significant changes. Given the scarcity of water resources and the degradation of ecological functions in arid and semi-arid regions of China, it is necessary to analyze the impact of landscape pattern changes on water conservation. Taking the Wei River Basin (WRB) as the research area, based on water balance equations, FRAGSTATS 4.2 software, and correlation analysis method, this study explored the spatiotemporal distribution characteristics of water conservation and landscape pattern in the WRB from 1990 to 2020, as well as the correlations between the two. The results showed that from 1990 to 2020: (1) the water conservation capacity (WCC) showed no insignificant change trend (P > 0.05) in sub water basins as well as the entire WRB, and it presented a spatial distribution pattern of more in the south and west, and less in the north and east; (2) the landscape diversity and fragmentation of the WRB have increased, and the patch shapes tended to be complex, while connectivity and agglomeration between patches decreased; (3) The WCC had significant negative correlations with the Contagion Index (CONTAG), connectivity index (COHESION), and largest patch index (LPI), while had significant positive correlations with landscape shape index (LSI), Shannon diversity index (SHDI), and separation index (SPLIT), indicating that the increase of landscape fragmentation and diversity was conducive to the improvement of WCC. In the process of ecological landscape planning and optimization, the fragmentation degree of landscape can be appropriately increased. (4) Vegetated grassland, forestland, and farmland were the main sources of WCC, with average WCC values of 81.90, 68.04, and 8.72 mm, respectively. NDVI of the watershed increased significantly (P < 0.01) during the research period and showed a significant positive correlation with WCC, indicating the promoting effect of increasing vegetation coverage on WCC. Relevant results may provide referential information for ecological restoration and optimization of landscape pattern in arid/semiarid regions.

Summer cover crop and temporary legume-cereal intercrop effects on soil microbial indicators, soil water and cash crop yields in a semi-arid environment

ContextFew studies have investigated summer cover crops as a replacement for bare fallow in semi-arid temperate regions, and summer cover crop impacts on soil biological function, water balances and subsequent winter crop yields in these regions remain largely unknown. ObjectiveThe 4-year field study aimed to determine the consequences of replacing a summer fallow with a summer growing cover crop on soil function, water balances and subsequent winter cash crop yields in a semi-arid region (486 mm mean annual rainfall) of the Australian southern cropping zone. MethodsA canola (Brassica napus)-wheat (Triticum aestivum L.) rotation with summer fallow was compared to the same rotation with either i) a single species (buckwheat, Fagopyrum esculentum L.) summer cover crop; ii) a four-species mixed brassica + cereal summer cover crop or iii) a cropping sequence with a temporary legume intercrop in the wheat phase of the rotation. Cover crop biomass production, soil water to a depth of 90 cm at cash crop sowing, crop yields and soil functional indices were quantified. ResultsCover crops produced 500–700 kg biomass ha−1 in the 2020 and 2021 seasons and 1000–2400 kg biomass ha−1 in 2022. The four-species mixed cover crop significantly lowered soil water (around 30 mm; P < 0.05) at the time of sowing the cash crop compared to the control and resulted in 15% reduction (P = 0.12) in canola seed yield in 2020, but no significant effect of cover crops of soil water and mineral N at sowing, or cash crop yields, were observed in subsequent seasons. In 2021 the temporary vetch intercrop produced > 1000 kg ha−1 biomass over 9 weeks, and led to a (non-significant) 10% wheat yield reduction. There was no significant effect of cover crop or temporary intercrop treatments on soil functions in the 2020 or 2021 seasons, but activities of several soil enzymes (leucine aminopeptidase, arylsulfatase and B-glucosidase; P < 0.05) and water-soluble carbon (P = 0.059) were significantly higher in the summer cover crop plots compared to control and intercrop plots in March 2022 following termination of the cover crops. These changes coincided with a significant increase in saturated hydraulic conductivity in the four-species cover crop plots in March 2022 compared to control plots. ImplicationsThe study did not provide any compelling evidence for benefits of summer cover crops over fallow in the short term (3–4 years). The relatively low cover crop biomass production over the 4-year study suggests summer cover crops are unlikely to substantially improve soil carbon levels in temperate, semi-arid cropping systems.