Washout Conditions Research Articles

Application of Guar Gum Treatment of Basalt Residual-Soil Shallow Slope in Early Ecological Restoration

This paper found that environmentally friendly guar gum biopolymers are helpful for stopping the erosion of basalt residual-soil shallow slopes, while also improving the problems of poor stability, difficult growth of early vegetation, and weak initial resistance to the rainfall scouring of these slopes under extreme climatic conditions. Then, to illustrate the effects of the guar gum treatment, laboratory tests have been conducted, including a soil strength test, water retention and water absorption tests, a disintegration test, and a simulated rainfall erosion test, and the pattern of its effect on vegetation growth has been explored. The results indicate that as the content of guar gum increases, both the cohesion and angle of internal friction exhibit a trend of first increasing and then decreasing; the angle of internal friction varies within a range of 21° to 26°. The evaporation rate, water absorption rate, and disintegration rate of this guar gum-treated soil were significantly reduced, while the cracking of the surface layer was significantly improved. The disintegration rate of the soil is only about 2%, as the guar gum content is greater than 1%. Moreover, there is no sign indicating that vegetation germination was affected by the guar gum, meaning that it maintains a favorable environment for vegetation to grow. Guar gum-cured slopes were significantly protected under heavy rainfall washout conditions, with a 94.85% reduction in total soil loss from the slope surface compared to untreated slopes. Since the pores of soil are filled with guar gum hydrogel, the erosion resistance of soil is greatly enhanced. The results of this study will provide a scientific basis for engineering the protection of shallow slopes of basalt residual soils.

Continuous H-B-E fermentation by Clostridium carboxidivorans: CO vs syngas

Leveraging renewable carbon-based resources for energy and chemical production is a promising approach to decrease reliance on fossil fuels. This entails a thermo/biotechnological procedure wherein bacteria, notably Clostridia, ferment syngas, converting CO or CO2 + H2 into Hexanol, Butanol and Ethanol (H-B-E fermentation). This work reports of Clostridium carboxidivorans performance in a stirred tank reactor continuously operated with respect to the gas and the cell/liquid phases. The primary objective was to assess acid and solvent production at pH 5.6 by feeding pure CO or synthetic syngas under gas flow differential conditions. Fermentation tests were conducted at four different dilution rates (DL) of the fresh medium in the range 0.034–0.25 h−1. The fermentation pathways of C. carboxidivorans were found to be nearly identical for both CO and syngas, with consistent growth and metabolite production at pH 5.6 within a range of dilution rates. Wash-out conditions were observed at a DL of 0.25 h−1 regardless of the carbon source. Ethanol was the predominant solvent produced, but a shift towards butanol production was observed with CO as the substrate and towards hexanol production with synthetic syngas. In particular, the maximum cell concentration (0.5 gDM/L) was obtained with pure CO at DL 0.05 h−1; the highest solvent productivity (60 mg/L*h of total solvent) was obtained at DL 0.17 h−1 by using synthetic syngas as C-source. The findings highlight the importance of substrate composition and operating conditions in syngas fermentation processes. These insights contribute to the optimization of syngas fermentation processes for biofuel and chemical production.

Activated carbon fibers functionalized with superhydrophilic coated pDA/TiO2/SiO2 with photoluminescent self-cleaning properties for efficient oil-water separation

The adhesion of high-viscosity oil contamination poses limitations on three-dimensional (3D) materials' practical use in treating oilfield-produced water (OPW). In this study, we developed a hybrid pDA/TiO2/SiO2 coating (PTS) on the surface of hydrophilic activated carbon (ACF1) through a combination of dopamine (DA) polymerization, ethyl orthosilicate (TEOS) hydrolysis, and the condensation of TiO2 nanoparticles (NPs) with SiO2 NPs. This coating was designed for gravity-based oil-water separation. The inherent porosity and generous pore size of ACF1-PTS conferred it an ultra-high permeation flux (pure water flux of 3.72 × 105 L∙m−2∙h−1), allowing it to effectively separate simulated oil-water mixtures and oil-water emulsions while maintaining exceptional permeation flux and oil rejection efficiency. When compared to cleaning methods involving ethanol aqueous solutions and NaClO, ultraviolet (UV) illumination cleaning proved superior, enabling oil-contaminated ACF1-PTS to exhibit remarkable flux recovery efficiency and oil-removal capabilities during cyclic separation of actual OPW. Furthermore, the ACF1-PTS material demonstrated impressive stability and durability when exposed to acidic environments (acid, alkali, and salt), robust hydraulic washout conditions, and 25-cycle tests. This study offers valuable insights and research avenues for the development of highly efficient and environmentally friendly 3D oil-water separation materials for the actual treatment of OPW.

Nutrient dynamics in water resources of productive flatland territories in the Pampean region of Argentina: evaluation at a watershed scale.

The Pampean plains in South America are well-known for their livestock and agricultural productivity. The peri-urban watershed of El Pescado Creek (Central-Eastern Argentina) has been significantly modified in the last few years due to local land-use changes. This work aims to analyze the dynamics of nutrient content associated with the surface water-groundwater relationship in this watershed and to define the trophic state of the watercourse. Sampling sites were selected for both surface water and groundwater analyses, and field surveys were carried out during the spring and summer of 2017. Handmade shallow groundwater wells were installed along the floodplain of the watercourse. Deep groundwater was analyzed in agricultural and livestock farms. In situ determinations included dissolved oxygen (DO), pH, electrical conductivity (EC), turbidity, transparency, and temperature measurements. Laboratory analyses included NO3--N, total nitrogen (TN), soluble reactive phosphorus (SRP), total phosphorus (TP), and phytobenthonic and phytoplanktonic chlorophyll-a. Results showed an increase in EC and nutrient concentration in the summer samples (both in surface water and shallow groundwater), along with higher turbidity of the surface water. Water flow was dissimilar between samplings (spring: 1.735 m3/s, summer: 0.065 m3/s), showing contrasting hydrological scenarios. Low wash-out conditions enhanced phytobenthonic algae biomass growth, turning most of the sites towards a eutrophic state in summer. Our results showed that the dynamics of nitrogen and phosphorus compounds in the watershed of El Pescado Creek depend on the hydrodynamic processes of the watershed, the different land-uses, and the chemical characteristics of these compounds. In order to develop sustainable management strategies, further understanding of nutrient concentrations effects, and the factors affecting them, must be done in this area of the Pampean region.

Differences in Sustained Cellular Effects of MET inhibitors Are Driven by Prolonged Target Engagement and Lysosomal Retention.

Intracellular distribution of drug compounds is dependent on physicochemical characteristics and may have a significant bearing on the extent of target occupancy and, ultimately, drug efficacy. We assessed differences in the physicochemical profiles of MET inhibitors capmatinib, crizotinib, savolitinib, and tepotinib and their effects on cell viability and MET phosphorylation under steady-state and washout conditions (to mimic an open organic system) in a human lung cancer cell line. To examine the differences of the underlying molecular mechanisms at the receptor level, we investigated the residence time at the kinase domain and the cellular target engagement. We found that the ranking of the drugs for cell viability was different under steady-state and washout conditions and that under washout conditions, tepotinib displayed the most potent inhibition of phosphorylated MET. Postwashout effects were correlated with the partitioning of the drug into acidic subcellular compartments such as lysosomes, and the tested MET inhibitors were grouped according to their ability to access lysosomes (crizotinib and tepotinib) or not (capmatinib and savolitinib). Reversible lysosomal retention may represent a valuable intracellular storage mechanism for MET inhibitors, enabling prolonged receptor occupancy in dynamic, open-physiologic systems and may act as a local drug reservoir. The use of washout conditions to simulate open systems and investigate intracellular drug distribution is a useful characterization step that deserves further investigation. SIGNIFICANCE STATEMENT: Generally, determination of potency and receptor occupancy is performed under steady-state conditions. In vivo conditions are more complex due to concentration differences between compartments and equilibrium processes. Experiments under steady state cannot explore effects such as sustained target inhibition. This study has shown that differences between MET inhibitors are observable by applying washout conditions to in vitro assays. This important finding applies to most compound classes and may inspire readers to rethink their assay designs in the future.

Dynamic visual acuity during asymmetric walking

Necessary for effective ambulation, head stability affords optimal conditions for the perception of visual information during dynamic tasks. This maintenance of head-in-space equilibrium is achieved, in part, by the attenuation of the high frequency impact shock resulting from ground contact. While a great deal of experimentation has been done on the matter during steady state locomotion, little is known about how locomotor asymmetry might affect head stability or dynamic visual acuity. In this study, fifteen participants walked on a split-belt treadmill while verbally reporting the orientation of a randomized Landolt-C optotype that was projected at heel strike. Participants were exposed to baseline, adaptation, and washout conditions, as characterized by belt speed ratios of 1:1, 1:3, and 1:1, respectively. Step length asymmetry, shock attenuation, high and low frequency head signal power, and dynamic visual acuity were averaged across the first and last fifty strides of each condition. Across the first fifty strides, step length asymmetry was significantly greater during adaptation than during baseline (p<0.001; d=2.442), and shock attenuation was significantly lower during adaptation than during baseline (p=0.041; d=-0.679). High frequency head signal power was significantly greater during adaptation than during baseline (p<0.001; d=-1.227), indicating a reduction in head stability. While dynamic visual acuity was not significantly lower during adaptation than during baseline (p=0.052), a moderate effect size suggests a decrease in the measure between the two conditions (d=0.653). Across the last fifty strides, many of the decrements observed between the baseline and adaptation conditions were greatly reduced. The results of this study indicate that the locomotor asymmetry imposed by the split-belt treadmill during early adaptation might lead to moderate decrements in shock attenuation, head stability, and dynamic visual acuity. Moreover, the relative reduction in magnitude of these decrements across the last fifty strides underscores the adaptive nature of the locomotor and visuomotor systems.

Source Apportionment, Hydrodynamic Influence, and Environmental Stress of Pharmaceuticals in a Microtidal Estuary with Multiple Outlets in South China.

Pharmaceutical residues in the environment are of great concern as ubiquitous emerging contaminants. This study investigated the presence of 40 pharmaceuticals in water and sediment of the Pearl River Estuary (PRE) in the wet season of 2020. Among psychiatric drugs, only diazepam was found in water samples while six of them were detected in the sediment. The Σantibiotics levels ranged from 6.18 to 35.9 ng/L and 2.63 to 140 ng/g dry weight in water and sediment samples, respectively. Fluoroquinolones and tetracyclines were found well settling in the outlet sediment, while sulfonamides could be released from disturbed sediment under stronger tidal wash-out conditions. After entering the marine waters, pharmaceuticals tended to deposit at the PRE mouth by the influence of the plume bulge and onshore invasion of deep shelf waters. Low ecological risks to the aquatic organisms and of causing antimicrobial resistance were identified. Likewise, hydrological modeling results revealed insignificant risks: erythromycin-H2O and sulfamethoxazole discharged through the outlets constituted 30.8% and 6.74% of their environmental capacity, respectively. Source apportionment revealed that pharmaceutical discharges through the Humen and Yamen outlets were predominantly of animal origin. Overall, our findings provide strategic insights on environmental regulations to further minimize the environmental stress of pharmaceuticals in the PRE.

Characterising sedimentation velocity of primary waste water solids and effluents

Sedimentation in waste water is a heavily studied topic, but mainly focused on hindered and compression settling in secondary sludge, a largely monodispersed solids, where bulk sedimentation velocity is effectively described by functions such as double Vesilind (Takacs). However, many waste water solids, including primary sludge and anaerobic digester effluent are polydispersed, for which application of velocity functions is not well understood. These systems are also subject to large concentration gradients, and poor availability of settling velocity functions has limited design and computational fluid dynamic (CFD) analysis of these units. In this work, we assess the use of various sedimentation functions in single and multi-dimensional domains, comparing model results against multiple batch settling tests at a range of high and low concentrations. Both solids concentration and sludge bed height (interface) over time are measured and compared. The method incorporates uncertainty analysis using Monte Carlo regression, DIRECT (dividing rectangles), and Newton optimisation. It was identified that a double Vesilind (Takacs) model was most effective in the dilute regime (<1%v/v), but could not effectively fit high solids concentrations (>1%v/v) without a substantial (50%) decrease in effective maximum sedimentation velocity (V0). Other parameters (Rh, Rp) did not change. A power law velocity model (Diehl) was significantly less predictive at low concentrations, and not significantly better at higher concentrations. The optimised model (with reduction in V0) was tested vs a standard (optimised) double Vesilind velocity model in a simple primary sedimentation unit, and resulted in deviation from -12% to +18% in solids capture prediction from underload to overload (washout) conditions, indicating that the effect is important in CFD based analysis of these systems.

Abstract 1019: The MET inhibitor tepotinib shows sustained effects on MET phosphorylation and viability in cellular washout experiments

Abstract It is well accepted that a drug's efficacy is modulated by the affinity of the drug to the target of interest and the stability of the protein/inhibitor complex. Additionally, drug efficacy is affected by pharmacokinetic properties. For example, lipophilic weak bases are able to easily permeate membranes at neutral pH in a non-protonated form, but also tend to accumulate in acidic compartments such as lysosomes. Consequently, lysosomes can act as a drug reservoir. The degree of lysosomal sequestration generally correlates with the pKa value of the basic function, and although the process is considered to be fully reversible, the degree and accessibility of the lysosomal reservoir heavily depends on compound-specific physicochemical properties. MET inhibitors such as crizotinib, capmatinib, savolitinib and tepotinib are providing the opportunity to study how differences in the physicochemical profiles of MET inhibitors affect the interplay of drug-target residence time and lysosomal storage, and how these factors translate into target inhibition and cell viability effects under washout conditions. Human lung cancer cell lines were treated with MET inhibitors across a range of concentrations for 1 h, cells were washed to remove compounds in the supernatant, and remaining compound activity was analyzed by measuring MET phosphorylation and lung cancer cell viability. Tepotinib showed persistent effects in both assays with significant differences observed between the tested MET inhibitors. In the viability assay comparing standard and washout conditions, the half-maximal inhibitory concentrations of tepotinib and crizotinib increased by a factor ranging from 10 to 20. In contrast, those of capmatinib and savolitinib increased by a factor &amp;gt;250, indicating a more sustained efficacy for tepotinib and crizotinib under washout conditions. Here, we present data on the underlying mechanisms affecting cellular efficacy under washout conditions, including residence time via surface plasmon resonance analysis, compound-partition coefficients via liquid chromatography with tandem mass spectrometry quantification, and lysosomal storage via a competitive dye displacement assay. This comparative analysis provides strong evidence that tepotinib demonstrates persistent efficacy in dynamic cellular systems, exceeding that of several other MET inhibitors. The underlying reason for this observation is probably the consequence of a long residence time of tepotinib (~20 h) combined with an optimal physicochemical profile allowing for a beneficial contribution of lysosomal retention. These in vitro findings may also explain the sustained pharmacokinetic and pharmacodynamic in vivo characteristics of tepotinib. Citation Format: Nina Berges, Jan Henrik Klug, Anna Eicher, Jennifer Loehr, Daniel Schwarz, Joerg Bomke, Birgitta Leuthner, Dominique Perrin, Oliver Schadt. The MET inhibitor tepotinib shows sustained effects on MET phosphorylation and viability in cellular washout experiments [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2021; 2021 Apr 10-15 and May 17-21. Philadelphia (PA): AACR; Cancer Res 2021;81(13_Suppl):Abstract nr 1019.

Preclinical modeling of chronic inhibition of the Parkinson\u2019s disease associated kinase LRRK2 reveals altered function of the endolysosomal system in vivo

The most common mutation in the Leucine-rich repeat kinase 2 gene (LRRK2), G2019S, causes familial Parkinson’s Disease (PD) and renders the encoded protein kinase hyperactive. While targeting LRRK2 activity is currently being tested in clinical trials as a therapeutic avenue for PD, to date, the molecular effects of chronic LRRK2 inhibition have not yet been examined in vivo. We evaluated the utility of newly available phospho-antibodies for Rab substrates and LRRK2 autophosphorylation to examine the pharmacodynamic response to treatment with the potent and specific LRRK2 inhibitor, MLi-2, in brain and peripheral tissue in G2019S LRRK2 knock-in mice. We report higher sensitivity of LRRK2 autophosphorylation to MLi-2 treatment and slower recovery in washout conditions compared to Rab GTPases phosphorylation, and we identify pS106 Rab12 as a robust readout of downstream LRRK2 activity across tissues. The downstream effects of long-term chronic LRRK2 inhibition in vivo were evaluated in G2019S LRRK2 knock-in mice by phospho- and total proteomic analyses following an in-diet administration of MLi-2 for 10 weeks. We observed significant alterations in endolysosomal and trafficking pathways in the kidney that were sensitive to MLi-2 treatment and were validated biochemically. Furthermore, a subtle but distinct biochemical signature affecting mitochondrial proteins was observed in brain tissue in the same animals that, again, was reverted by kinase inhibition. Proteomic analysis in the lung did not detect any major pathway of dysregulation that would be indicative of pulmonary impairment. This is the first study to examine the molecular underpinnings of chronic LRRK2 inhibition in a preclinical in vivo PD model and highlights cellular processes that may be influenced by therapeutic strategies aimed at restoring LRRK2 physiological activity in PD patients.

Cultivation of aerobic granular sludge for the treatment of food-processing wastewater and the impact on membrane filtration properties.

A laboratory-scale sequencing batch reactor was operated for approximately 300 days, divided into four periods based on the feeding strategy, to develop stable aerobic granular sludge (AGS) while treating chocolate processing wastewater. Application of a prolonged mixed anaerobic feeding was not sufficient to develop AGS and reach stable reactor performance. Through the application of a partially non-mixed and a partially mixed feeding strategy, the reactor performance was increased and stable AGS formation was established characterized by low diluted sludge volume index (D)SVI DSVI10,30) values of 78 ± 27 mL·g-1 and 52 ± 17 mL·g-1, respectively, and a capillary suction time/mixed liquor suspended solids value of 0.9 sec·(g·L-1)-1. The membrane bioreactor (MBR) filtration tests showed a reduction of the fouling rate (FR) and an increase of the sustainable flux (SF0.5) for AGS compared to flocs treating the same industrial wastewater. The SF0.5 (FR > 0.5 mbar·min-1) for the flocs was 10 L·(m2·h)-1 while for AGS the SF0.5 is higher than 45 L·(m2·h)-1 because the FR did not exceed 0.1 mbar·min-1. Additionally, the AGS showed reduced irreversible fouling tendencies due to pore blocking. Our results underline the need for an increased substrate gradient during anaerobic feeding for the development and long-term maintenance of AGS under minimum wash-out conditions. The AGS-MBR filtration performance also shows strong advantages compared to a floccular MBR system due to a high increase of the SF0.5 and reduced reversible and irreversible fouling.

Direct antimicrobial activity of cationic amphipathic peptide WLBU2 against Staphylococcus aureus biofilms is enhanced in physiologic buffered saline.

Periprosthetic joint infection of total knee arthroplasties represents a major challenge to the field of orthopedic surgery. These infections are commonly associated with antibiotic-tolerant Staphylococcus aureus biofilms. Engineered cationic amphipathic peptide WLBU2 has shown the ability to kill antibiotic-resistant pathogens and drug-tolerant bacterial biofilms. The novelty of using WLBU2 during the direct irrigation and debridement of periprosthetic joint infections led our group to investigate the optimal washout conditions for treatment of S. aureus biofilms. S. aureus mature biofilms were grown on metal implant material and treated with WLBU2 dissolved in differing irrigation solvents. Mature biofilms were treated both in vitro as well as in a periprosthetic joint infection murine model. WLBU2 activity against S. aureus biofilms was increased when dissolved indiphosphate-bufferedsaline (dPBS) with pH of 7.0 compared withnormal saline with pH of 5.5. WLBU2 activity was decreased in acidic dPBS and increased in alkaline dPBS. WLBU2 activity could be decreased in hypertonic dPBS and increased in hypotonic dPBS. WLBU2 dissolved in less acidic dPBS displayed increased efficacy in treating periprosthetic joint infection (PJI) implants ex vivo. WLBU2 demonstrated the ability to eliminate PJI associated S. aureus biofilms on arthroplasty material. The efficacy of engineered cationic amphipathic peptide WLBU2 for intraoperative elimination of S. aureus biofilms can be further optimized when kept in a less acidic and more physiologic pH adjusted saline. Understanding optimal physical washout conditions are vital for the success of WLBU2 in treating S. aureus biofilms in PJI clinical trials going forward.

Dynamics of ripples superimposed on a sand ridge on a tideless shoreface

The presence of ripples superimposed on an active sand ridge is the most usual situation under low-to moderate-energy conditions in the wave-dominated, tideless Ebro Delta coast. Four types of small-scale bedforms were identified, from the critical threshold of sediment motion to wash-out conditions: (i) small undulations with η < 0.5 cm and λ ~8 cm, formed as a precursor of wave ripples when the Shields parameter was just below the theoretical critical; (ii) 2D wave ripples with η = 1.2 cm and λ = 7–10 cm; (iii) mixed 2D/3D current-dominated ripples with a maximum of η ~1.5 cm and λ = 10–15 cm; and (iv) 3D wave-current ripples with a maximum of η ~2.2 cm and λ = 7–20 cm. Ripple degradation was observed to occur when the energy of the hydrodynamic regime increased (wash-out conditions) and under low-energy hydrodynamic conditions, when ripples progressively decayed mainly as a consequence of biological activity. The wave ripples were static, while the current-dominated ripples migrated towards the SE at a rate of ~10 cm/h.The potential role of ripple migration as an additional long-term mechanism of sediment transport is addressed. On the Ebro Delta shoreface, 3D ripple migration follows the direction of sand ridge migration towards the SE. The respective migration rates and their differences in size support the hypothesis that a subordinate part of sand ridge migration can result from the contribution of ripple migration under low to moderate regimes, suggesting that in specific environments the dynamics of small-scale bedforms can play a subordinate but not negligible role in the evolution of larger bedforms.

Carrot leaves improve color and xanthophyll content of egg yolk in laying hens but are not as effective as commercially available marigold fortificant

Yellow and orange egg yolks are good sources of xanthophyll carotenoids, consumption of which is associated with health benefits, such as cancer prevention, eye health, and bone health. Industrial feed fortificants used to improve egg yolk color and carotenoid concentration typically are derived from marigold flowers. Green leafy vegetables are also concentrated sources of the xanthophylls lutein and zeaxanthin (L+Z), but they have not been rigorously evaluated in laying hen feeds as a yolk colorant. The addition of food manufacturing byproducts, including carrot leaves, to animal feed is a promoted method of improving animal nutrition. The ability of dehydrated carrot leaves to improve egg yolk color and L+Z concentration was evaluated by feeding laying hens (n = 40) white maize-based feeds fortified with 2 different dehydrated carrot leaves, marigold as a positive control, or no fortificant as a negative control for 28 D. After a 7-D washout period, the hens were separated into 4 groups, and eggs were collected every other day. Yolks were analyzed by using a portable colorimeter to define the color space and by ultra-performance liquid chromatography to determine the carotenoid profile. Carotenoid concentration rapidly declined from day 0 to 8, confirming adequate washout conditions. The white maize negative control (WM) day 28 lutein concentration (3.59 ± 0.51nmol/g) was significantly less than orange-carrot leaf-treated (OCL) (5.34 ± 0.36nmol/g) and red-carrot leaf-treated hens (RCL) (5.92 ± 1.00nmol/g) in addition to the marigold-treated hens (MG). However, MG was significantly higher than both leaf-treated groups. From day 8 (3.93 ± 0.74nmol/g) to 28 (9.32 ± 1.66nmol/g), MG had the largest increase in lutein and was the only treatment to surpass day 0 initial concentrations (8.50 ± 1.64nmol/g). A similar trend was observed for zeaxanthin and was reflected in the color space.

Discovery of Covalent CDK14 Inhibitors with Pan-TAIRE Family Specificity

Cyclin-dependent kinase 14 (CDK14) and other TAIRE family kinases (CDKs 15-18) are proteins that lack functional annotation but are frequent off-targets of clinical kinase inhibitors. In this study we develop and characterize FMF-04-159-2, a tool compound that specifically targets CDK14 covalently and possesses a TAIRE kinase-biased selectivity profile. This tool compound and its reversible analog were used to characterize the cellular consequences of covalent CDK14 inhibition, including an unbiased investigation using phospho-proteomics. To reduce confounding off-target activity, washout conditions were used to deconvolute CDK14-specific effects. This investigation suggested that CDK14 plays a supporting role in cell-cycle regulation, particularly mitotic progression, and identified putative CDK14 substrates. Together, these results represent an important step forward in understanding the cellular consequences of inhibiting CDK14 kinase activity.

Evaluation and application of a method for estimating nasal end-tidal O2 fraction while administering supplemental O2.

This paper describes a method for estimating the oxygen enhanced end-tidal fraction of oxygen (FetOe), the end-tidal fraction of oxygen (FetO2) that is raised by administering supplemental oxygen. The paper has two purposes: the first is to evaluate the method's accuracy on the bench and in volunteers; the second purpose is to demonstrate how to apply the method to compare two techniques of oxygen administration. The method estimates FetOe by analyzing expired oxygen as oxygen washes out of the lung. The method for estimating FetOe was first validated using a bench simulation in which tracheal oxygen was measured directly. Then it was evaluated in 30 healthy volunteers and compared to the bench simulation. Bland-Altman analysis compared calculated and observed FetOe/FetO2 measurements. After the method was evaluated, it was implemented to compare the FetOe obtained when administering oxygen using two different techniques (pulsed and continuous flow). A total of eighteen breath washout conditions were evaluated on the bench. FetOe estimates and tracheal FetO2 had a mean difference of - 0.016 FO2 with 95% limits of agreement from - 0.048 to 0.016 FO2. Thirteen breath washouts per volunteer were analyzed. Extrapolated and observed FetO2 had a mean difference of - 0.001 FO2 with 95% limits of agreement from - 0.006 to 0.004 FO2. Pulsed flow oxygen (PFO) achieved the same FetOe values as continuous flow oxygen (CFO) using 32.1% ± 2.27% (mean ± SD) of the CFO rate. This paper has demonstrated that the method estimates FetO2 enhanced by administering supplemental oxygen with clinically insignificant differences. This paper has also shown that PFO can obtain FetO2 similar to CFO using approximately one-third of the oxygen volume. After evaluating this method, we conclude that the method provides useful estimates of nasal FetO2 enhanced by supplemental oxygen administration.

Transport Scaling of Dune Dimensions in Shallow Flows

AbstractDune dimensions in sand‐bedded rivers are often thought to scale with flow depth (h), with height (H) scaling as 1/6h and length (L) as 5h, even though substantial scatter about the relations has been observed. Transport stage has been shown to affect bedform geometry, but this control is often ignored in favor of depth scaling relations. Here, we use a series of flume experiments to systematically test controls on dune dimensions and variability. Experiments involved three sets of runs under five constant transport stages, ranging threshold to washout conditions, at three different flow depths. The mobile bed was repeatedly scanned during a 10‐hr equilibrium period to derive mean values and quantify the variability. The results show that dune‐depth scaling is not consistent because of a transport stage effect. Dune height increases with transport stage until a point when H decreases. Length remains nearly constant with transport stage until further increases in transport stage leads to lengthening. In general, dunes grow higher when significant bedload transport occurs but become flatter and longer in the presence of substantial suspension. Ultimately, dunes scale with transport stage, which is a function of slope, grain size, and h. The results are used to derive transport stage relations to guide predictions of dune dimensions in rivers and reconstructions of paleoflows based on dimensions estimated from cross strata. The relations incorporate the nonlinear response of dune dimensions with transport stage and provide metrics of uncertainty to include in predictions.

Characteristic Analysis of Downhole Pressure Signal while Drilling under Drillstring Washout Conditions

The identification of drillstring washout conditions during drilling is mostly based on standpipe pressure, drilling fluid flow rate or turbine RPM. These methods are inaccurate or not applicable to other drilling methods. It is difficult to find the drillstring washout conditions in time. While the downhole measurement technology can provide important informations for real-time discrimination of downhole conditions, there are few studies on the fluctuation characteristics of pressure signals while drilling under drillstring washout conditions. Using finite element software to simulate wellbore pressure distribution under drillstring washout conditions, Based on the self-developed near-bit multi-parameter measurement tool, the downhole drilling pressure signal is collected, and the signal density analysis method such as probability density, wavelet, spectrum and time domain is used to discuss the relationship between the fluctuation characteristics of the pressure signal while drilling and drillstring washout. The results show that, (1) Before and after washout occur, the pressure distribution inside and outside the drill string changed significantly. After washout occur, there is fluid outflow at the puncture, and the fluid flow velocity in the drill string and the annulus at the bottomhole decrease,the pressure in the drillstring and the annulus smaller than before the puncture. (2) The time domain and frequency domain characteristics of pressure while drilling can judge the occurrence of the drillstring washout. When washout occurs, the annulus pressure near the drill bit drops sharply, and the pressure signal spectrum produces a low frequency band. (3) The wavelet analysis and probability density characteristics of the pressure while drilling can be used as an auxiliary basis for the drillstring washout. The wavelet detail signal of the pressure signal can judge the pressure fluctuation, and the probability density characteristic can judge the change of the pressure amplitude. The fluctuation characteristics of the drilling pressure signal under drillstring washout obtained in this paper can provide reference and guidance for the identification of complex conditions in the drilling process.

Development of aerobic granular sludge under tropical climate conditions: The key role of inoculum adaptation under reduced sludge washout for stable granulation

Aerobic granular sludge (AGS) is a promising technology for wastewater treatment. However, the success of the process depends on the formation of stable granular biomass, which is associated with the microbiological aspects of the sludge and reactor operating conditions. In this study, the development of AGS from a poor nitrifying flocculent sludge obtained in a sewage treatment plant designed only for organic matter removal was assessed in a sequencing batch reactor (SBR) under tropical climate conditions (temperatures of 28 ± 4 °C). The results showed that, despite the alternating anaerobic-aerobic conditions during the granules selection phase under high sludge washout rates (low settling time), readily biodegradable organic matter was mainly removed aerobically. The formed granules were unstable, exhibiting a substantial amount of filaments and pasty consistency. The biomass characteristics (e.g., sludge volume index, density, diameter and settling velocity) were negatively impacted as complete granulation was reached, while biomass loss and degranulation became inevitable. Poor nitrification and no enhanced biological phosphate removal (EBPR) were observed. Implementation of a new operational strategy incorporating an adaptation of the seed sludge under reduced washout conditions (high settling time) prior to the granules selection stage enabled most of the influent organics to be removed anaerobically. Besides allowing a feast-famine regime to be established in the reactor, the sludge acclimation phase favoured the development of slow-growing organisms and suppressed the appearance of filamentous-like structures. Fast-settling granules with regular shape remained stable in the long-term, while high ammonium (>95%) and total nitrogen removal (>90%) was obtained. However, EBPR activity was very unstable, most likely due to the high temperatures. The findings of this study are important for the spreading of the AGS technology worldwide, especially in developing countries where the conditions are different in all aspects.

Determination of ultra-trace sulfur in high-purity metals by isotope dilution inductively coupled plasma sector field mass spectrometry combined with chemical separation procedure

The analytical method of ultra-trace sulfur (S) in high-purity metal by isotope dilution inductively coupled plasma mass spectrometry (ID-ICP-MS) combined with chemical separation procedure was developed in the present study. In order to determine the ultra-trace S in high-purity metal, a chemical separation with alumina column was carried out before ICP-MS measurement. This method enabled to prevent the polyatomic ion interference arising from the metal matrix and the signal suppression derived from the space charge effect in ICP-MS. In order to achieve high sensitive analysis, an ICP-sector field mass spectrometer (ICP-SFMS) was used. The isolation of polyatomic ion interference with respect to S was also carried out in medium-resolution mode. In addition, measurement conditions including detector dead time, which affects the precision and accuracy of the isotope dilution method, and washout conditions that were employed to reduce memory effects were optimized. The developed method was validated by the determination of S in a high-purity iron reference material (JSS-001–4). The analytical result obtained by the developed method (1.86 mg kg−1 ± 0.12 mg kg−1 (k = 2)) was in good agreement with the certified value (1.90 mg kg−1 ± 0.42 mg kg−1). The method was also applied to the determination of S in high-purity zinc, revealing a content of 0.08 mg kg−1 ± 0.08 mg kg−1 (k = 2). Since the developed method enabled the determination of ultra-trace S at μg kg−1 level in the high-purity zinc, it is expected to be useful for high sensitive and accurate determination of ultra-trace S in high-purity metals.