Warner-Bratzler Shear Force Values Research Articles

Understanding tenderness variability and ageing changes in buffalo meat: biochemical, ultrastructural and proteome characterization

Understanding of biological impact of proteome profile on meat quality is vital for developing different approaches to improve meat quality. Present study was conducted to unravel the differences in biochemical, ultrastructural and proteome profile of longissimus dorsi muscle between buffaloes (Bubalus bubalis) of different age groups (young v. old). Higher (P<0.05) myofibrillar and total protein extractability, muscle fibre diameter, and Warner-Bratzler shear force (WBSF) values was observed in old buffalo meat relative to meat from young buffaloes. Scanning electron microscopy photographs revealed reduced fibre size with increased inter-myofibrillar space in young compared with old buffalo meat. Transmission electron microscopy results revealed longer sarcomeres in young buffalo meat relative to meat from old buffaloes. Proteomic characterization using two-dimensional gel electrophoresis (2DE) found 93 differentially expressed proteins between old and young buffalo meat. Proteome analysis using 2DE revealed 191 and 95 differentially expressed protein spots after 6 days of ageing in young and old buffalo meat, respectively. The matrix assisted laser desorption ionization time-of flight/time-of flight mass spectrometry (MALDI-TOF/TOF MS) analysis of selected gel spots helped in identifying molecular markers of tenderness mainly consisting of structural proteins. Protein biomarkers identified in the present study have the potential to differentiate meat from young and old buffaloes and pave the way for optimizing strategies for improved buffalo meat quality.

Aging implications on fresh muscle traits of Certified Angus Beef steaks.

Vacuum-packaged Certified Angus Beef (CAB) subprimals ( = 72) that included the longissimus thoracis (LT), longissimus lumborum (LL), gluteus medius (GM), and infraspinatus (IF) muscles were purchased from a major beef packing facility. Subprimals were allocated to 1 of 3 aging periods (14, 28, or 42 d) and aged at 2°C. After aging, 5 steaks were cut from each subprimal and assigned to pH, water-holding capacity, Warner-Bratzler shear force (WBSF), cooked color, cooking yield, cooking loss, and sensory panel analysis. Infraspinatus steaks were more tender ( < 0.05) than all other steaks, and subprimals aged 14 d had greater ( < 0.05) WBSF values than the other 2 aging periods, regardless of muscle. Water-holding capacity and cook yield were greater ( < 0.05) for LL and LT than IF and GM steaks, whereas purge loss was greater ( < 0.05) for IF and GM than LL and LT steaks. Throughout the aging periods, pH declined for all muscle groups, with IF steaks having the greatest ( < 0.05) pH values among all muscles. Among IF steaks, sensory evaluations of all attributes did not ( ≥ 0.26) differ across aging periods; yet among LT steaks, consumers rated those aged 14 d greater ( < 0.05) in overall impression than LT steaks aged 28 and 42 d. Among LT steaks, those aged 14 d received greater ( < 0.05) flavor ratings than LT steaks subjected to longer aging periods, and LT steaks aged 14 d received the greatest ( < 0.05) overall impression, with consumers giving greater ( < 0.05) overall impression scores to LT steaks aged 42 d over those aged 28 d. Aging period had no effect ( ≥ 0.017) on consumer ratings for flavor, tenderness, juiciness, or overall impression of LL steaks. Among GM steaks, consumers rated steaks aged 14 and 28 d more ( < 0.05) flavorful than those aged 42 d, and consumer ratings for overall impression were greater ( < 0.05) for GM steaks aged 28 d than for GM steaks aged 42 d; however, consumers failed ( = 0.035) to note differences in tenderness scores of GM steaks in response to aging period. Furthermore, consumers indicated a greater ( < 0.05) likelihood to purchase LT steaks aged 14 d over LT steaks aged 28 d, LL steaks aged 42 d over LL steaks aged 14 d, and GM steaks aged 14 and 28 d over GM steaks aged 42 d. These results indicate that consumers struggled to identify steak flavor attributes and suggest that the benefit of aging for premium beef products does not offer a tremendous sensory advantage to the consumer.

Evaluation of Meat Color and Physiochemical Characteristics in Forequarter Muscles of Holstein Steers.

The beef forequarter muscle comprises approximately 52% of carcass weight. The objective of this study was to evaluate the physiochemical characteristics and meat color from forequarter muscle of Holstein steers. Fifteen forequarter muscles were trimmed of external connective tissue and fat. An experimental group of eight Holstein steers was assessed using meat color, water-holding capacity, drip loss, and Warner–Bratzler shear force value at the same quality grade. The M. omotransversarius (0.45 kg) had the highest (p<0.05) lightness (L*) value, whereas the M. teres major (0.4 kg) and M. triceps brachii (caput laterale) (0.52 kg) had the lowest (p<0.05) values. The M. semispanitus capitus (1.48 kg), which is a neck muscle, had the highest values for both redness (a*) and yellowness (b*), whereas the lowest (p<0.05) values were for the M. teres major. The M. omotransversarius, M. latissimus dorsi (1.68 kg), and M. rhomboideus (1.2 kg) were ranked high (p<0.05) in water-holding capacity. The drip loss value was the highest for the M. longissimus dorsi thoracis (p<0.05; 1.86 kg), while the M. infraspinatus (2.28 kg), M. supraspinatus (1.38 kg), M. brachiocephalicus (1.01 kg), and M. pectoralis superficialis (1.18 kg) had the lowest (p<0.05). The Warner–Bratzler shear force value indicated that the M. pectoralis profundus (3.39 kg), M. omotransversarius, and M. brachiocephalicus were the toughest (p<0.05), whereas the M. subscapularis (0.86 kg), M. longissimus dorsi thoracis, M. teres major, and M. infraspinatus were the most tender cuts (p<0.05). Here, muscle type explained most of the variability in the forequarter physiochemical characteristics. Thus, our findings suggest that these muscle profile data will allow for more informed decisions when selecting individual muscles to produce value-added products from Holstein steers.

Tenderness assessments of top loin steaks from retail markets in four U.S. cities.

The purpose of this study was to evaluate the tenderness of beef loin steaks from retail markets in 4 U.S. cities. Beef top loin steaks ( = 1,613) were obtained for Warner-Bratzler shear force (WBSF), slice shear force (SSF), and consumer sensory determinations. Personnel at 4 universities (California Polytechnic State University, Colorado State University, University of Missouri, and Texas A&M University) conducted the study over a 12-mo period. Enhanced/blade-tenderized top loin steaks had the lowest ( < 0.05) WBSF and SSF values, whereas nonenhanced top loin, bone-in steaks had the highest ( < 0.05) WBSF and SSF values. Enhanced/blade-tenderized top loin steaks received the highest ( < 0.05) ratings by consumers for palatability scores, whereas nonenhanced top loin, bone-in steaks had the lowest ( < 0.05) consumer panelist ratings. The USDA quality grade did have an effect ( < 0.05) on the tenderness of nonenhanced steaks but did not affect ( > 0.05) steaks that were enhanced/blade tenderized. The WBSF values and consumer sensory values for top loin steaks were comparable to the 2010 National Beef Tenderness Survey, signifying that no drastic changes in tenderness have occurred due to changes in antemortem or postmortem conditions.

Effect of forage type with or without corn supplementation on animal performance, beef fatty acid composition, and palatability.

Thirty-two steers were used to examine forage type (legumes [ and ] vs. grasses [ and ]) with or without individual corn grain supplementation (0 vs. 0.75% of live weight [LW]/d) on beef fatty acid composition and palatability. In each year, steers ( = 16/yr) were randomly assigned to forage type ( = 8/forage type per yr) and to supplementation treatments within forage type ( = 4/supplementation treatment/forage type per yr). Forage types (grasses vs. legumes) were replicated in 2 paddocks of perennial and annual forage type pastures. A mixed model was developed with forage type, corn grain supplementation, and the 2-way interaction as fixed effects and 2 different error terms, one for testing forage and another for testing grain supplement and grain supplement × forage interaction. Corn grain supplementation increased ( = 0.01) ADG by 0.29 kg/d and final LW by 13 kg. Hot carcass weight, dressing percentage (DP), and KPH were greater ( < 0.05) for steers supplemented with corn grain. Carcasses from steers grazing legumes had greater ( = 0.04) DP compared with carcasses from steers grazing grasses. Alpha-linolenic acid concentration was higher ( < 0.05) in LM of steers grazing legumes than in LM of steers grazing grasses, both without supplementation. Supplementation decreased ( < 0.05) linolenic acid levels for both forage types; however, the magnitude of this reduction was greater for legumes than for grasses. The ratio of -6 to -3 PUFA was greater ( = 0.03) in the LM of corn grain-supplemented steers than in the LM of nonsupplemented steers. Supplementation of corn grain decreased ( < 0.05) the percentage of odd-chain fatty acids and increased ( < 0.05) the percentage of MUFA in the LM. Warner-Bratzler shear force values were not altered ( > 0.05) by forage type, supplementation, or the 2-way interaction. Beef finished on legumes had greater concentrations of -3 PUFA, whereas beef supplemented with corn grain had a greater ratio of -6 to -3 fatty acids. On a gravimetric basis (mg/100 g LM), -3 PUFA and CLA contents were not altered with supplementation, indicating that corn grain can be supplemented at this level in a forage-finishing beef system without negative consequences on perceived beneficial fatty acids.

Three dimensional chemometric analyses of hyperspectral images for beef tenderness forecasting

A prototype on-line hyperspectral imaging system (λ = 400–1000 nm) was developed and used to acquire images of exposed ribeye muscle on hanging beef carcasses (n = 274) at 2-day postmortem in a commercial beef packing plant. After image acquisition, a strip steak was cut from each carcass and vacuum packaged. After aging for 14 days, the steaks were cooked and Warner-Bratzler shear force values were collected as a measure of tenderness. Four different principal component analysis-based dimensionality reduction methods were implemented to reduce information redundancy in beef hyperspectral images. Textural features extracted from the 2-day hyperspectral images were modeled using Fisher's linear discriminant (FLD), support vector machines (SVM), and decision tree (DT) models to predict 14-day aged, cooked beef tenderness. Based on a true validation procedure using 101 samples, the FLD model yielded a tender certification accuracy of 86.7%. In addition, wavelengths corresponding to myoglobin and its derivatives (541, 577, and 635 nm), beef aging (541, 577, 635, 756, and 980 nm), protein (910 nm), fat (928 nm), and water (739, 756, and 988 nm) were identified.

Physical meat quality and chemical composition of the Longissimus thoracis of entire and immunocastrated pigs fed varying dietary protein levels with and without ractopamine hydrochloride

Physical and chemical attributes of the Longissimus thoracis (LT) of 96 PIC© entire (E) and immunocastrated (C) pigs were evaluated. The study followed a 2×2×3 factorial design where three diets of low, medium and high proteins (7.50, 9.79 and 12.07g digestible lysine/kg) were fed either with (10mg/kg) or without ractopamine (RAC) for the last 28days of growth. Vaccination of C occurred at 16 and 20weeks and slaughtering at 24weeks of age. The LTs were analysed for moisture, protein, fat and ash contents as well as CIE L*, a*, b* colour, drip loss, cooking loss and Warner–Bratzler shear force (WBSF). Various sex and protein interactions were observed for LT protein content, L* values and WBSF. Cooking loss was decreased in C and by the medium protein diet. Feeding RAC increased WBSF values, whilst decreasing a* and b* values. However, the differences observed are minor and might be considered negligible when evaluated by a consumer.

Effects of Ultrasound Treatment on Connective Tissue Collagen and Meat Quality of Beef Semitendinosus Muscle

AbstractThe effects of low‐frequency, high‐power ultrasound (40 kHz, 1,500 W) on meat quality and connective tissue collagen of beef semitendinosus muscle were assessed. Beef steaks were sonicated for 10, 20, 30, 40, 50 or 60 min. The effects of ultrasound on exudate yield, water‐loss rate, cooking loss, meat tenderness, and connective tissue and collagen properties were assessed. The results revealed that ultrasound increased meat exudate and water‐loss rates and reduced Warner–Bratzler shear force values. However, ultrasound had no effect on cooking loss or insoluble collagen content and little effects on collagen content and solubility. The mechanical strength of connective tissue decreased in ultrasound‐treated beef samples. Following ultrasound treatment of ≥10 min, muscle fibers shrank, the endomysium was disrupted and the perimysium thickness decreased. Protein aggregates formed in the extracellular space. Low‐frequency, high‐power ultrasound had significant effects on meat texture and connective tissue properties.Practical ApplicationsIntramuscular connective tissue (IMCT) and collagen are related to meat quality, especially meat tenderness and texture. Ultrasound treatment enhances tenderness and sensory attributes of meat by changing cellular structures. However, there are limited data on the effectiveness of ultrasound on meat quality from the viewpoint of IMCT. In this study, we reported the meat quality attributes affected by changes in connective tissue and collagen as a result of ultrasound treatment. The results obtained in this study would be useful for the scientific community and the meat industry.

Influence of extended aging on beef quality characteristics and sensory perception of steaks from the gluteus medius and longissimus lumborum

The objective was to determine the influence of post-fabrication aging (2, 14, 21, 42, and 63days) on beef quality characteristics and consumer sensory perception of gluteus medius (GM) and longissimus lumborum (LL) steaks. Lipid oxidation and aerobic plate counts increased (P<0.05) with longer aging periods and retail display times. An aging period by day of retail display interaction (P<0.05) was observed for a* and b* values for both muscles and L* values for the LL. Warner–Bratzler shear force values decreased (P<0.05) with longer aging for the LL, while no difference was observed for the GM. Consumer panel results demonstrated that longer aging periods increased (P<0.05) tenderness of both muscles. Our results indicate that extended aging reduces retail color stability yet has positive effects on consumer perception of tenderness of beef loin muscles.

Effect of cut type and post-mortem ageing on the technological quality, textural profile and sensory characteristics of horse meat

The present study was conducted to investigate the effects of cut type and ageing period on the technological quality, colour, texture and sensory characteristics of horse meat. Nine different cuts: loin, shoulder chuck roll, shank, outside round, tenderloin, shoulder clod, brisket, top round and striploin from 28-month-old female horses were used in the present investigation. The cuts were randomly assigned into 2, 10, 20 and 30 days’ ageing groups and ageing was carried out in a chilling room (2 ± 2°C). The results showed that there were significant (P &lt; 0.05) differences among the cuts for the drip loss, pH and cooking loss at all ageing days; particularly, striploin had the lowest pH and highest drip loss whereas shoulder chuck roll had the highest pH value. Ageing significantly (P &lt; 0.05) increased in pH and drip loss in most cuts with the highest drip loss ~5.17% in striploin and the highest cooking loss ~9.27% in shoulder chuck roll after 30 days of ageing. At Day 2 of ageing, no differences in Warner–Bratzler shear force values occurred between the cuts (P &gt; 0.05). Ageing only decreased Warner–Bratzler shear force in the loin and outside round (P &lt; 0.05). Colour parameters also revealed differences between cuts and ageing periods, particularly the L* (lightness) values ranged between the cuts from 34.38 to 37.14, 34.0 to 37.24, 33.24 to 37.07, and 34.7 to 38.92 at Days 2, 10, 20 and 30 of ageing, respectively; similarly the a* (redness) values ranged between the cuts from 16.76 to 17.81, 16.61 to 17.85, 17.46 to 19.46, and 17.72 to 20.47 at Days 2, 10, 20 and 30 of ageing, respectively. For the compression, the lowest hardness values (4.41–5.73 kg) were found in tenderloin at all ageing days. Furthermore, the scores for tenderness, flavour, juiciness and acceptability were different (P &lt; 0.05) among the cuts at all ageing days. Ageing improved flavour of loin, shank and striploin, and juiciness of tenderloin and striploin whereas it decreased the juiciness and acceptability scores of some cuts (e.g. shoulder chuck roll). Thus, the present data provide not only useful information for horse meat producers but also important databases for further investigations.

Effect of Aging on the Physicochemical, Textural, Microbial and Proteome Changes in Emu (D romaius novaehollandiae ) Meat Under Different Packaging Conditions

The effect of aging on the physicochemical, textural, microbial and proteome characteristics of emu (Dromaius novaehollandiae) meat was studied under aerobic packaging (AP) and vacuum packaging (VP) conditions at 4 ± 1C for 9 and 15 days, respectively. Improvement (P < 0.05) in water-holding capacity, myofibrillar fragmentation index and protein extractability with aging was observed in emu meat cubes under both AP and VP conditions. Reduction (P < 0.05) in Warner-Bratzler shear force values was observed on the 6th and 15th day of aging compared with the 0th day in the AP and VP samples, respectively. The sodium dodecyl sulfate–polyacrylamide gel electrophoresis analysis revealed the appearance of 30-kDa protein bands, indicating extensive proteolysis on the 6th day and 9th day of aging in the AP and VP samples, respectively. Proteome analysis using two-dimensional gel electrophoresis revealed significant (P < 0.01) changes in the number of differentially expressed protein spots in the AP and VP samples during aging. Practical Applications Emus are classified as ratites along with ostriches and rheas and are becoming increasingly popular as a source of low-fat, low-cholesterol, iron-rich red meat alternative. The emu industry is looking for science-based information in order to successfully produce and market emu meat. The present work provides information on emu meat quality, aging process, ultrastructure, proteome characteristics and microbial changes. This paper also discusses about the different packaging methods and the shelf life of emu meat. Considering the increasing demand for new source of animal proteins around the world, this paper provides significant information to all ostrich, rhea and emu meat processors.

The inclusion of Duroc breed in maternal line affects pork quality and fatty acid profile

The aim of this study was to evaluate the effect of including different percentages of Duroc (D) breed in maternal line [Landrace (LR)×Large White (LW); LR×(LW×D); LR×D] and gender on meat quality and intramuscular (IMF) and subcutaneous (SCF) fatty acid composition. No significant differences were found among dam lines in ultimate pH, L* values and drip and cooking losses. There were higher percentages of saturated fatty acids in LR×D and LR×(LW×D) lines and higher percentages of polyunsaturated fatty acids in LR×LW line in IMF and SCF. Also, LR×D line produced pork with a lower Warner–Bratzler shear force values and higher IMF content and potential of lipid oxidation. Furthermore, the L*, a* and b* values and drip loss were greater in pork from entire males than females. The IMF and SCF of females were more monounsaturated and less polyunsaturated than those from entire males.

Effects of forage silage species on yearling growth performance, carcass and meat quality, and nutrient composition in a forage based beef production system

Berthiaume, R., Lafrenière, C., Girard, C., Campbell, C. P., Pivotto, L. M. and Mandell, I. B. 2015. Effects of forage silage species on yearling growth performance, carcass and meat quality, and nutrient composition in a forage based beef production system. Can. J. Anim. Sci. 95: 173–187. Forty weaned, crossbred beef calves (predominantly Angus and Simmental) were forage-finished using all-silage diets (red clover–timothy versus tall fescue) to examine forage species’ effects on growth performance, carcass traits, meat quality, and nutrient composition. Weaned calves (257 d of age) were forage-finished using red clover–timothy or tall fescue silage and harvested at approximately 1 yr of age. During carcass processing, one side from each carcass was covered with a polyliner to examine if reducing rates of chilling could compensate for limited fat cover expected from low dietary energy contents fed, and limited time on feed. Longissimus thoracis, semimembranosus, and semitendinosus steaks were aged 10, 14, and 21 d to examine effects on Warner–Bratzler shear force values while fatty acid and vitamin B12 composition were determined on 10-d-aged steaks. Average daily gain, feed efficiency, hot carcass weights, and longissimus muscle area were greater (P&lt;0.03) when cattle were fed red clover–timothy versus tall fescue silage, most likely due to the higher protein content of red clover–timothy silage. Shear force was greater (P&lt;0.002) in steaks from all muscles evaluated from cattle fed tall fescue versus red clover–timothy silage. In comparison to 10-d-aged steaks, 14 d of ageing were needed to reduce (P&lt;0.001) shear force for longissimus steaks, while 21 d of ageing were needed to reduce (P&lt;0.001) shear force for semitendinosus steaks. Use of a polyliner decreased (P=0.0001) the rate of temperature decline at selected carcass sites, but did not reduce shear force values. The percent of n-3 fatty acids and polyunsaturated fatty acid/saturated fatty acid ratio were greater (P&lt;0.04) in longissimus from cattle fed red clover–timothy versus feeding tall fescue silage. Feeding red clover–timothy silage improved growth performance, carcass, shear force, and fatty acid composition traits versus feeding tall fescue silage.

Genetic parameters and genetic correlations among triacylglycerol and phospholipid fractions in Angus cattle.

The objective of this study was to estimate genetic parameters for intramuscular fatty acids from triacylglycerol (TAG) and phospholipid (PL) fractions in beef LM tissue. Longissimus muscle samples were obtained from 1,833 Angus cattle to determine the intramuscular fatty acid composition for 31 lipids and lipid classes from TAG and PL fractions and were classified by structure into saturated (SFA), monounsaturated (MUFA), polyunsaturated (PUFA), omega-3 (n-3), and omega-6 (n-6) fatty acids. An atherogenic index (AI) was also determined as a measure of the unsaturated fatty acid to SFA ratio. Restricted maximum likelihood methods combined with pedigree data were used to estimate variance components with the WOMBAT software package. Heritability estimates ranged from 0.00 to 0.63 for the major classes of fatty acids. Heritability estimates differed between the TAG and PL fractions, with higher estimates for TAG up to 0.64 and lower estimates for PL that ranged from 0.00 to 0.14. Phenotypic and genetic correlations among individual fatty acids were determined for the TAG fraction as well as among carcass traits, including rib eye area, numerical marbling score, yield grade, ether fat, and Warner-Bratzler shear force value. Strong negative or positive genetic correlations were observed among individual fatty acids in the TAG fraction, which ranged from -0.99 to 0.97 ( < 0.05). Moderate correlations between carcass traits and fatty acids from the TAG fraction ranged from -0.43 to 0.32 ( < 0.05). These results indicate that fatty acids prominent in beef tissues show significant genetic variation as well as genetic relationships with carcass traits.

Comparison of Warner–Bratzler shear force values between round and square cross-section cores from cooked beef and pork Longissimus muscle

This study was conducted to investigate the effect of core sampling on Warner–Bratzler shear force evaluations of beef and pork loins (Longissimus thoracis et lumborum muscles) and to determine the relationship between them. Steaks of 2.54cm from beef and pork loins were cooked and five round cross-section cores and five square cross-section cores of each steak were taken for shear force evaluation. Core sampling influenced both beef and pork shear force values with higher (P<0.05) average values and standard deviations for square cross-section cores. There was a strong and linear relationship (P<0.01) between round and square cross-section cores for beef (R2=0.78), pork (R2=0.70) and for beef+pork (R2=0.82) samples. These results indicate that it is feasible to use square cross-section cores in Warner–Bratzler shear force protocol as an alternative and potential method to standardize sampling for shear force measurements.

Influence of diet on serum glucose and insulin concentrations, insulin receptor concentration in adipose and muscle tissues, and oxygen consumption in liver and muscle in gilts

Raw samples of 14 muscles: Mm. biceps femoris (BF), quadriceps femoris (CF), diaphragm (DI), flexor digitorum (FD), gluteus medius (GM), infraspinatus (IE), longissimus lumborum (LL), longissimus thoracis (LT), psoas major (PM), pectoralis profundus (PP), semimembranosus (SM), semitendinosus (ST), sternomandibularis (STER) and triceps brachii (TB) from four Swiss Brown (485±15 days old) young bull carcasses and weighing approximately 300 kg were evaluated for some chemical and physical properties. PM (2.11 kg) and DI (2.24 kg) were the muscles which had the lowest Warner–Bratzler shear force values, while PP (6.66 kg) had the greatest shear force (P<0.05). FD and IE muscles had the highest concentration of total collagen content while PM and DI had the lowest (P<0.05) contents, TB and IE muscles presented the highest insoluble collagen concentration while PM and LT had the lowest (P<0.05) contents. High positive correlation between total collagen content and Warner–Bratzler shear force of raw samples was found (r=0.723; P<0.01) and between insoluble collagen content and Warner–Bratzler shear force was (r=0.661; P<0.01). Significant differences (P<0.05) were observed among muscles for differential scanning calorimetry, sarcomere length, pH and colour parameters.

Tenderization potential of Hanwoo beef muscles from carcasses with differed genders and loin intramuscular fat content levels during post mortem ageing.

Carcasses from Hanwoo steers (n = 15) and cows (n = 15) were classified into three groups: group 1 (G1), the carcasses had 10% to < 11.5% intramuscular fat (IMF) in loin muscles; group 2 (G2), the carcasses had 13% to < 4.5% IMF in loin muscles; and group 3(G3), the carcasses had 17% to < 18.5% IMF in loin muscles. These were used to evaluate the effects of gender and carcass group on quality traits and Warner-Bratzler shear force (WBSF) of Psoas major (PM), Longissimus thoracis (LT), Longissimus lumborum (LL), Longus colli (LC), Supraspinatus (SS), Latissimus dorsi (LAD), Semimembranosus (SM), Quadriceps femoris (QF), Biceps femoris (BF) and Semitendinosus (ST) muscles. Our results showed that pH values of LT, LL, LC, BF and QF muscles were lower in steers than in cows (P < 0.05). Water holding capacity (WHC) was found higher in LC, SS, LAD and QF muscles of steers (P < 0.05). At day 2 of ageing, gender affected the WBSF values of only PM, LD and QF muscles in G1, and QF muscle in G3; however, with additional ageing, the gender effect was observed for most of the muscles. Most muscles showed ageing responses; however, the rates of ageing response significantly varied depending on gender and carcass groups. The muscles of G1 and G2 had generally higher tenderization potentials than those of G3. Furthermore, most muscles in G3 had generally lower WBSF values than in G1 and G2. These results clearly indicate that ageing has a significant effect on quality and WBSF of beef muscles, and the classification by loin IMF level may be useful for prediction of the tenderness of other muscles.

Freezing, thawing and aging improves the tenderness of chuck muscles from young bulls of different phenotypes

We evaluated eleven chuck muscles from phenotypically selected Brahman (BR, n =10) and Charolais (CH, n =10) young bull carcasses for the effect of freezing, thawing, and aging for 2, 9, 16, and 23 days on Warner-Bratzler shear force(WBSF) Splenius, infraspinatus, teres major, rhomboideus, and subscapularis from both phenotypes, and complexus from CH had tender (≤ 40 N) 9 d WBSF values. No further improvement in WBSF was observed beyond 9 days of aging. Overall, we observed freezing, thawing and aging of several chuck muscles from young bulls significantly improves beef tenderness. Further studies would help optimizing this tenderization strategy and assess its potential impact on other meat quality attributes.

Freezing, thawing and aging improves the tenderness of chuck muscles from young bulls of different phenotypes

We evaluated eleven chuck muscles from phenotypically selected Brahman (BR, n =10) and Charolais (CH, n =10) young bull carcasses for the effect of freezing, thawing, and aging for 2, 9, 16, and 23 days on Warner-Bratzler shear force (WBSF). Splenius , infraspinatus , teres major , rhomboideus , and subscapularis from both phenotypes, and complexus from CH had tender (≤ 40 N) 9 d WBSF values. No further improvement in WBSF was observed beyond 9 days of aging. Overall, we observed freezing, thawing and aging of several chuck muscles from young bulls significantly improves beef tenderness. Further studies would help optimizing this tenderization strategy and assess its potential impact on other meat quality attributes .

Sensory evaluation of tender beef strip loin steaks of varying marbling levels and quality treatments

The palatability of tender [Warner-Bratzler shear force values <33.34N (3.4kg)] beef strip loins of 10 different treatments [USDA Prime, High Choice (upper 1/3 Choice), Low Choice (lower 1/3 Choice), Select, Standard, Australian Wagyu, American Wagyu, Holstein Select, Holstein Top Choice (upper 2/3 Choice) and Grass-finished] was evaluated by consumers and a trained flavor panel. In general, tenderness, juiciness, flavor, and overall liking ratings as well as acceptability percentage for each trait, increased with increased fat levels. Moreover, overall liking was highly correlated (P<0.01) with flavor liking (r=0.96) as well as fat percentage (r=0.79). Beef flavor scores were positively associated (P<0.01) with fat-like (r=0.67) and umami (r=0.59) flavors. Fat level was the primary driver of beef flavor acceptability in all samples when no undesirable off-flavors were present.