PurposeWith the goal to indicate the importance of human–nature connection in designing workplaces, the authors investigated the effects of direct and indirect biophilic indicators on the well-being and performance of employees of an organization. The main question is what is the effect of biophilic components on the well-being and performance of employees.Design/methodology/approachTo answer the research questions, the authors designed and validated an instrument to evaluate correlation between biophilic components with well-being and performance. The participants include 160 employees of one organization. For the data analysis, both descriptive statistical and inferential statistics were used. Pearson's correlation test and ANOVA were conducted to explain the relationship between variables.FindingsThe results indicated that biophilic components have significant positive relationships with the well-being of the employees, but no significant relationship was found between biophilic components and the performance of the employees. Using the ANOVA test, biophilic indicators that have a positive and significant effect on the well-being and performance of employees were determined. The natural scenery and warm colors has significant positive effects on social well-being, and the usage of natural landscape posters in the working rooms has a positive and significant relationship with psychological well-being. The natural lighting has a positive and significant relationship with the employees' counterproductive work behavior.Research limitations/implicationsMost of the questionnaires of this research were filled electronically because of the health protocols during pandemic. Almost 70% of the organization's employees had less than 5 years of work experience, which can be considered one of the interfering factors. In addition, the authors only used the questionnaire as a tool for collecting information in this study. There are other forms of information that can be explored through observations, interviews or experiments. Another limitation of this study is the lack of significant diversity in the investigated environments. Finally, the numbers of male and female participants of this study were not equal. The authors suggest studying both genders with an almost equal ratio so that the differences and needs of both groups can be understood.Practical implicationsThe designed questionnaire is a validated instrument that can be used by other researchers to evaluate the biophilic design of workplace. These findings can also be useful for managers, companies and organizations in making decisions to improve the working environment and increase the well-being and performance of their employees.Social implicationsThe social effects of biophilic design in the workplace are significant as well. The use of biophilic components can foster a sense of connection between employees and their workplace, creating a positive social environment that supports collaboration and communication. Additionally, a biophilic workplace can promote a positive company culture that values the health and well-being of its employees, which can contribute to employee retention and satisfaction.Originality/valueIn this study, to determine the effects of the biophilic indicators on dimensions of employees' well-being and performance, an instrument is designed and validated.