Land users and policy makers recognize importance of soil health and these types of evaluations are welcomed within sustainable land management. The aim of this study was to establish minimum and maximum values of soil health index across the breadth of agricultural used soils of Slovakia. Core objectives included (1) identifying the range of soil health benchmarks; (2) defining benchmarks for different land uses, and (3) defining benchmarks of soil health index for main groups of agricultural soils in Slovakia. These benchmarks represent the first soil health metrics of their kind in Slovakia using data from 266 locations. The soil health index (SHI) approach has been used as a quantitative tool to establish linkage between soil health and soil ecosystem services. We recorded the highest average SHI value in the very warm climatic region of the Slovak Republic, where the majority of agriculturally used arable soils (with an optimal pH value without contamination) are located. The locality with lowest SHI value is in a slightly warm area on soil used as arable soil with a very small depth of humus horizon, with by the pH value in an acidic area, and with a high content of clay. The typical SHI in humus-rich soil groups of Slovakia (Mollic Fluvisols, Chernozems and Cutanhic Luvisols) is higher contrasted with the typical SHI in other soil groups of Slovakia.