It is observed that beneficial microorganisms are used effectively in agricultural practices that are important for human and environmental health. These microorganisms, which are known biological agents and microbial fertilizers, are important in terms of plant development, quality and reduction of input, thanks to the increase in the amount of nutrients in plants. In this study, it was goaled to determine the effects of EM.FPE and EM.5 as foliar sprays and EM.A as soil application on shoot length, some fruit parameters and yield and leaf nutrient elements in Chandler walnut variety. In the experiment, the longest shoots were measured with 55 cm in EM.A application. Similarly, the highest values in nut weight (12.85 g) and yield (2.28 kg tree-1) were obtained in the same application. All applications positively affected kernel browning. These were found to increase leaf macro and micro nutrient content, except Cu and Fe.