In modern era, thermo-migration of microorganisms is an appealing research topic in bio-nanotechnology, bio engineering, and biomedical. In this context, a mathematical model describing thermo-bioconvection of Sutterby nanofluid flow including motile gyrotactic microorganisms near a perforated Riga plate under the physical impacts of heat radiation, and Arrhenius kinetics associated with binary chemical reaction is formulated and simulated here. The Darcy-Forchheimer (DF) law is applied to determine the porosity of porous media. The Grinberg term is taken for the Lorentz force owing to the parallel Riga plate wall. Appropriate translations are discharged to turn the constitutive partial differential equations (PDEs) into ordinary differential equations (ODEs), that are numerically computed by opting the Runge–Kutta-Fehlberg method (RKF-45) along with shooting strategy. The physical insights of various controlling variables on the transport profiles, Sherwood number, Nusselt number, and microorganisms density number are exemplified through requisite graphs and tables. It must be admitted that with enlarging Darcy number, the nanofluid velocity declines, while Forchheimer number has opposite consequence on it. The motile microorganisms density sharply decreases for improving values of activation parameter. The present modeling would provide preliminary guidances in a variety of biotechnological and industrial applications.