Many in vitro studies focus on effects of wall shear stress (WSS) and wall shear stress gradient (WSSG) on endothelial cells, which are linked to the initiation and progression of atherosclerosis in the arterial system. Limitation in available flow chambers with a constant WSSG in the testing region makes it difficult to quantify cellular responses to WSSG. The current study proposes and characterizes a type of converging parallel plate flow chamber (PPFC) featuring a constant gradient of WSS. A simple formula was derived for the curvature of side walls, which relates WSSG to flow rate (Q), height of the PPFC (h), length of the convergent section (L), its widths at the entrance (w0) and exit (w1). CFD simulation of flow in the chamber is carried out. Constant WSSG is observed in most regions of the top and bottom plates except those in close proximity of side walls. A change in Q or h induces equally proportional changes in WSS and WSSG whereas an alteration in the ratio between w0 and w1 results in a more significant change in WSSG than that in WSS. The current design makes possible an easy quantification of WSSG on endothelial cells in the flow chamber.