Background: Enterococci have emerged recently as a nosocomial pathogen especially in immunocompromized individuals and in intensive care units. Objectives: This study was carried out to detect incidence of VRE to detect genotypes of vancomycin resistance Enteroccci by PCR and to do a comparison between the results MIC of VRE and PCR results. Methodology: This study was conducted from May 2013 to January 2014. It was conducted on 560 hospitalized patients in Zagazig University Hospitals including 270 males and 290 female. The specimens were collected from patients suffering from any infections. Examination of isolates by conventional methods and API 20 Strep system. Susceptibility patterns of VRE isolates to antibiotics were determined by both disc diffusion method and E test. The genotypic detection of vancomycin resistance among enterococcal isolates was done by using polymerase chain reaction (PCR). Results: This study showed that the overall incidence of vancomycin resistance among Enterococci was 35.2% (57 isolates). The study showed that 38 of isolates (66.7%) had VanA gene and their MIC were more than 64 µg/ml. Twelve (21.05%) of isolates had VanB gene and their MIC also were more than 64 µg/ml. Five of isolates (8.8%) had VanC gene. MIC of these 5 isolates were between 4 and 16 µg/ml. Our study showed that VanA – PCR had the highest sensitivity to detect resistant isolates in comparison to VanB – PCR and VanC – PCR. Conclusion: The incidece of VRE in Zagazig University Hospitals was significantly high. The commonest genotype of vancomycin resistance in Enterococci was the VanA genotype. Other genotypes seen were VanB and VanC (low level resistance to vancomycin). VanA – PCR had the highest sensitivity to detect resistant isolates in comparison to VanB – PCR and VanC – PCR.