Plain Language SummaryMandarin speakers with poststroke spastic dysarthria often have vowel production deficits that affect articulation and speech intelligibility. Vowel production is influenced by many factors. This study explored the acoustic effects of speaker sex, tones, and speech samples and their interactions on vowel production in Mandarin speakers with poststroke spastic dysarthria and confirmed the existence of these effects. We found that regardless of the speech samples or tones, there were the reduced range of articulatory movements, centralized vowel production, and prolonged vowel duration in dysarthria compared to healthy people. The most sensitive and useful analysis measures were the vowel articulation index and vowel duration. Specific influencing factors (speaker sex, speech sample, and tone) of vowel production need to be taken seriously by speech and language pathologists and considered in the assessment and rehabilitation.