Responses of a geometry-induced separation bubble (GISB) and a pressure-induced separation bubble (PISB) at enhanced levels of free-stream turbulence (FST) have experimentally been investigated for a comparative study using the particle image velocimetry (PIV) technique. The outlines of separation bubbles based on the dividing streamlines are self-similar for different levels of FST and Reynolds numbers. The spectral analyses of the time-resolved PIV data show that the vortex shedding frequency of a separated shear layer remains unchanged for the GISB cases even with an enhanced level of FST. In contrast, it is different for the PISB cases. We propose a criterion that determines whether the frequency will remain the same even for the cases with FST. Linear stability analyses reveal that the inviscid-inflectional instability dominates the transition process, and the linear stages of transition are not completely bypassed even at an enhanced level of FST. The most amplified frequencies, while scaling with the displacement thickness and the boundary layer edge velocity, collapse in a single curve for all the cases. Furthermore, measurements in the spanwise plane show that the streamwise velocity streak/Klebanof mode at an enhanced level of FST is not a general flow feature for all types of separation bubbles. However, at an enhanced level of FST for the PISB case, the boundary layer streaks are found to distort the two-dimensional vortex structure associated with the Kelvin–Helmholtz instability, eventually leading to a three-dimensional $\varLambda$ -like structure in the spanwise plane.
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