Background: Abdominal aortic aneurysms (AAA) are common, particularly among individuals over age 65, with a prevalence of 9%. To better understand the natural history of AAAs, one critical knowledge gap must be filled to elucidate the role of vortical flow in vascular degradation and thrombosis formation. We aimed to induce various types of vortical flow with aneurysm-like hemodynamic environments using a novel aorta stenosis model. Such a model will enable us to investigate the interplays between disturbed hemodynamics and vascular pathological remodeling. Methods: In this study, the hemodynamics and geometry of the aorta in rabbits were used to simulate the physiological flow conditions after two adjacent stenoses were created virtually with varying degrees of area reduction (30-70%) and spacing (5-6.8 mm). A physiological blood flow (rate) waveform measured in rabbits using ultrasound Doppler (normalized to 3.0 mL/s) was prescribed to the inlet (i.e., descending aorta), and zero pressure boundary conditions were applied at two outlets (i.e., iliac arteries). 3D computational fluid dynamics (CFD) simulation in five different stenosis configurations was performed using commercial software (Fluent, Version 20, Ansys Inc., PA). In-house software assessed the evolution of flow vortices over three distinct regions post stenosis using an established method. The total volume of vortex flow, the number of vortices, and the phase-to-phase overlap of vortex flows within different post-stenosis regions were derived to characterize the vortex flow. Results: Quantitative and qualitative flow assessments were performed for all five CFD models. Firstly, in all models, we found consistent patterns of the three vortex flow parameters, indicating that the adjacent stenoses induced three different hemodynamic zones in the post-stenosis regions, namely, stable vortical flow (after first stenosis), transient flow (after second stenosis), unstable vortical flow (further distal to second stenosis). Moreover, increased stenosis degree or reduced spacing led to reduced vortex flow number but increased total volume of vortex flow. The distinctions of the three regions were most significant in the model with 50% and then 30% stenoses, and with a 5 mm (center-to-center) spacing between two stenoses. Conclusions: CFD simulations can offer a powerful tool to predict complex flows using subject-specific vasculature. Such a flow simulation platform is valuable for guiding experimental design or therapy in various diseases. We demonstrated that it is feasible to adjust and achieve multiple types of vortical flows with the novel stenosis model. The three zones with distinct hemodynamic environments allow us to investigate the vascular remodeling regulated by the specifics of the eddies. Our ongoing work is implementing optimized aorta stenosis models to preclinical or in vitro studies to reveal the interplays between varying disturbed flow and aneurysm remodeling. We acknowledge funding support from NIH/NIBIB (R01-EB029570A1) and the Health Research Institute of Michigan Technological University. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.
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