The geochemistry of arc rocks provides a window to probe compositional variability in mantle wedge and slab-derived inputs. However, quantitative estimation of their contribution is a great challenge. Here we report an integrated study for determination of variable contributions to early Paleozoic arc plutons (the Mafan diorites and gabbros), from the northern Hong'an orogen, central China. Zircon grains from the diorites yielded crystallization age of 445 ± 3 Ma, whereas the gabbros have zircon UPb age of 432 ± 3 Ma. Although both the two mafic rocks suites exhibit typical arc-like trace element distribution patterns, they show a series of differences in stable Li–Mo–O and radiogenic Sr–Nd–Hf isotope compositions, as well as major and trace element features. Geochemically, the diorites are medium-K calc-alkaline series with high and positive whole-rock εNd(t) (+3.8 to +4.5) and zircon εHf(t) values (+12.4 to +14.3), and normal mantle-like zircon δ18O values (5.1 ± 0.2 ‰). Contrarily, the gabbros are tholeiitic and have relatively low whole-rock εNd(t) (+ 2.3 to +3.1) and zircon εHf(t) (+5.0 to +8.5) values with normal mantle-like zircon δ18O values (5.4 ± 0.3 ‰). The diorites have δ98Mo values of −0.15 to −0.03 ‰, while the gabbros have δ98Mo values of −0.28 to −0.12 ‰. These values are slightly higher than those of the mid-ocean ridge basalts (MORB) (−0.20 ± 0.01 ‰). Whereas, δ7Li values of the studied diorites (2.15–4.49 ‰) and gabbros (2.89–5.33 ‰) are mostly consistent within the range of MORB. In combination with the above results, the primary magma of diorites was inferred to be derived from 1 to 5% partial melting of depleted lithospheric mantle metasomatized by slab-derived fluid with minor sediment-derived melts. While the gabbros were suggested to be generated by 5–10% partial melting of more depleted lithospheric mantle that had undergone perhaps extra 0.3% melt extraction, which had further been metasomatized by more subducted materials. In summary, both the slab-derived components and nature of the mantle wedge sources are quantitatively constrained for the mafic rocks in the Mafan plutons. Our new data suggest that the Mafan plutons exhibited the Mariana arc-affinity and represented products of an intra-oceanic subduction system in the northern Hong'an orogen. Combined with previously published data for the early Paleozoic mafic magmatic rocks along the Qinling-Tongbai-Hong'an orogenic belt, there exists an oceanic arc in the east (Hong'an orogen) and an Andean-type continental arc in the west (Qinling-Tongbai orogen).