VLDL-C Concentrations Research Articles

Ameliorative potentials of quercetin and omega-3 fatty acids on cadmium-induced alterations in serum lipids in rats

Aim/Background: Dyslipidaemia is one of the diseases with high mortality worldwide, about 45% of the world population is being affected leading to cardiovascular disorders. Cadmium is a ubiquitous toxic heavy metal pollutant in the biosphere which has been implicated as one of the factors responsible for dyslipidaemia. This work was therefore conducted to ascertain the effects of omega-3 and/or quercetin on cadmium- induced dyslipidaemia in rats. Methods: Forty two (42) Wistar rats were assigned into 7 groups of 6 rats each and fed for 8 weeks with normal rat feed and drinking water. The treatment groups took either of cadmium chloride, omega-3 fatty acid and/or quercetin. The sham control groups 1 and 2 took olive oil (0.1 mL/kg body wt. o.p) and DMSO (1.0 mL/kg body wt. o.p) respectively. Blood samples were collected for measurement of some biochemical parameters. Results: Results obtained showed significant decrease in HDL-c, while TC, TG and VLDL-c concentrations increased significantly (p<0.05) in Cd groups compared with control, omega-3 and/or quercetin groups. Cadmium administration also increased LDL-c conc. and atherogenic indices (CRR, AC and AIP) significantly (p<0.05) compared to Omega-3 group. These changes were ameliorated in omega-3 and/or quercetin treated groups. Conclusion: Administration of quercetin or omega-3 ameliorates the adverse effects of Cd on serum lipids (LDL-c and HDL-c), antherogenic indices (CRR, AC & AIP), preventing dyslipidaemia that would have resulted in cardiovascular and other metabolic disorders. Quercetin or omega-3 had variable effects in ameliorating the adversity of Cadmium. However, combination of both quercetin and omega-3 produced a better ameliorating effect than when administered singly.

Estimation of relationship between serum lipids level and colorectal carcinoma: a cross-sectional study in VIMSAR, Odisha

The fourth most prevalent cancer-related cause of death worldwide is colorectal cancer. Some authors have suggested that among other risk factors, the serum lipid profile is one of the etiological risk factors. Therefore, measurement of serum lipid in this illness can establish a relationship between a healthy diet and colorectal cancer.To thoroughly research the connection between blood lipid levels and colon cancer outcomes in an effort to discover any tips for preventing this deadly condition.Patients who were hospitalised to the General Surgery department at VIMSAR, Burla between December 2020 and October 2022 were divided into two groups for the research. 40 instances of patients with colorectal cancer were randomly chosen as the case group, and 40 cases of patients with other diseases—diseases other than colorectal cancer—were chosen as the controls group. The cases were all diagnosed with carcinoma of various regions of the colon and rectum at various stages and in various age groups. Both the case and control groups' blood concentrations of TC, HDL-C, VLDL-C, LDL-C, and TG were determined for comparison and analysis.Regardless of sex, location, or stage of the malignancy, the blood levels of TC, TG, and LDL-C were lower in the tumour group than they were in the control group. On site comparison, right sided cases were much less common than left sided carcinomas, and the drop in the level of serum TC was statistically significant. However, significant differences were detected in the serum levels of the aforementioned parameters in advanced cases, which only included Dukes' C1 and C2. A negative relationship exists between serum TC, TG, and LDL-C levels and colorectal cancer. In order to design a more curative course of action for treating this dreadful illness, it may be used for follow-up treatment of colorectal carcinoma cases as well as a low level of these serum parameters preceding the disease or in early tumours. Our study only included a small number of people, thus more research and a cohort study based on the entire population are needed to fully understand this association.

Diets Enriched in Sugar, Refined, or Whole Grain Differentially Influence Plasma Cholesterol Concentrations and Cholesterol Metabolism Pathways with Concurrent Changes in Bile Acid Profile and Gut Microbiota Composition in ApoE-/- Mice.

This study aimed to compare the effects of diets enriched in sugar, refined grain (RG), or whole grain (WG) on circulating cholesterol concentrations and established and emerging mechanisms regulating cholesterol metabolism. Forty-four male ApoE-/- mice aged 8 weeks were randomly fed an isocaloric sugar-, RG-, or WG-enriched diet for 12 weeks. Compared to WG-enriched diet, fasting plasma LDL-C and HDL-C concentrations were higher and the mRNA expression of intestinal LXR-α was lower in sugar- and RG-enriched diets; plasma TC, non-HDL-C, TG and VLDL-C concentrations, and cecal concentrations of lithocholic acid were higher and the mRNA expression of intestinal ABCG5 was lower in sugar-enriched diet, and the mRNA expression of hepatic IDOL and cecal concentrations of lithocholic and deoxycholic acids was higher in RG-enriched diet. The relative abundance of Akkermansia, Clostridia_UCG-014, Alistipes, and Alloprevotella, which were lower in sugar- and/or RG- than in WG-enriched diet, had inverse correlations with fasting plasma cholesterol concentrations or cecal concentrations of secondary bile acids and positive correlations with gene expressions in intestinal cholesterol efflux. Conversely, the relative abundance of Lactobacillus, Lachnoclostridium, Lachnospiraceae_NK4A136_group, Colidextribacter, and Helicobacter had reverse correlations. Both sugar- and RG-enriched diets had unfavorable effects on cholesterol concentrations; yet, their effects on the gene expressions of cholesterol efflux, uptake, bile acid synthesis, and bile acid concentrations were distinctive and could be partially attributable to the concurrent changes in gut microbiota.

Effects of Coconut Oil and Palm Oil on Growth, Rumen Microbiota, and Fatty Acid Profile of Suckling Calves.

This study aimed to evaluate the effects of coconut oil and palm oil in milk replacer (MR) on the growth performance, blood lipids, rumen fermentation, rumen microbiota, and fatty acid profile of hepatic and muscle of suckling calves. Thirty-six Holstein male calves were randomly assigned to three treatments. Three milk replacers containing different fat sources were as follows: control group (CON, milk fat), coconut oil group (CCO, coconut oil powder as fat), and palm oil group (PLO, palm oil powder as fat). Calves were weighed and blood sampled at 14, 28, 42, and 56 days old, respectively, and the feed intake and fecal score were recorded daily. Fat sources in milk replacers had no effects on body weight, ADG, DMI, fecal score, or days of abnormal fecal in suckling calves among the three groups, while the PLO group tended to decrease starter intake compared with the other groups. Serum concentrations of TC, HDL-C, LDL-C, and VLDL-C in the CCO group increased compared with those of the CON group. Palm oil also decreased the serum GLU concentration of calves but had no effects on serum lipids compared with milk fat. Coconut oil or palm oil had no effects on rumen fermentation, rumen chyme enzyme activity, rumen bacterial community richness and diversity, and dominant phyla and genera when compared with milk fat. However, compared with the CON group, the CCO group increased the proportion of MCFAs and n-6 PUFAs, and decreased the proportion of UFAs and MUFAs in liver tissue, while the PLO group increased the proportion of PUFAs and decreased the proportion of n-3 PUFAs in liver tissue. In addition, compared with the CON group, the CCO group increased the proportion of MCFAs, and decreased the proportion of UFAs and n-3 PUFAs in longissimus dorsi, while the PLO group increased the proportion of PUFAs and decreased the proportion of n-3 PUFAs in longissimus dorsi. In conclusion, compared with milk fat, coconut oil or palm oil in MR had no effects on growth performance, rumen fermentation, and rumen microflora but significantly increased serum lipids concentration and changed some proportions of MCFAs and PUFAs in liver and longissimus dorsi in suckling calves. These results indicate that coconut oil or palm oil as the sole fat source for MRs has no adverse effect on calf rumen fermentation and rumen microbiota but has a detrimental effect on n-3 PUFAs deposition in the liver and longissimus dorsi muscle.

Effect of Urtica dioica leaves and Moringa oleifera leaves and seeds on glucose and lipid Profile in the blood of males albino rats exposed to toxicity by cadmium chloride and lead acetate

The current study in Veterinary Medicine College in kirkuk University and kirkuk hosbital for period from 2-2-2015 end 28-12-2015, and designed to observe and test the preventive and Therapy effects for powder of Urtica dioica leaves and Moringa oleifera leaves and seeds in healthy adult males albino rats that exposed to cadmium chloride (5 mg/kg of body weight) and lead acetate (50 mg/kg of body weight) and the amount of the transaction over the period (30) days on Profile of blood lipids, randomly divided into (18) groups, each group included (5) animals. Giving the powder of Urtica dioica leaves and Moringa oleifera seeds led significant decrease (P˂0.05) in concentrations of TC, TG, LDL-C, VLDL-C and significant increase (P˂0.05) in HDL-C, While administration of Moringa oleifera leaf powder for animal groups to the same previous results, whereas did not show significant differences (p˃0.05) in glucose, TG, HDL-C, malondia-ldehyde (MDA) and VLDL-C all of these in comparison with the normal control group. The groups that given cadmium chloride and lead acetate caused a significant increase in the TG, LDL-C, VLDL-C, malondialdehyde (MDA) and significant decrease in HDL-C in comparison with the normal control group. But compariing of these groups with the groups that administrated only Urtica dioica leaves and Moringa oleifera leaves and seeds led to significant decrease in HDL-C. and a significant increase in the TC, TG, LDL-C, VLDL-C. The groups of animals which treated with the powder of these plants under study before and after of exposed to each of cadmium chloride and lead acetate showed a positive effect on the Profile of blood lipids.

Biomedical consequences of elevated cholesterol-containing lipoproteins and apolipoproteins on cardiovascular and non-cardiovascular outcomes

BackgroundHigher concentrations of cholesterol-containing low-density lipoprotein (LDL-C) increase the risk of cardiovascular disease (CVD). The association of LDL-C with non-CVD traits remains unclear, as are the possible independent contributions of other cholesterol-containing lipoproteins and apolipoproteins.MethodsNuclear magnetic resonance spectroscopy was used to measure the cholesterol content of high density (HDL-C), very low-density (VLDL-C), intermediate-density (IDL-C), as well as low-density lipoprotein fractions, the apolipoproteins Apo-A1 and Apo-B, as well as total triglycerides (TG), remnant-cholesterol (Rem-Chol) and total cholesterol (TC). The causal effects of these exposures were assessed against 33 outcomes using univariable and multivariable Mendelian randomization (MR).ResultsThe majority of cholesterol containing lipoproteins and apolipoproteins affect coronary heart disease (CHD), carotid intima-media thickness, carotid plaque, C-reactive protein (CRP) and blood pressure. Multivariable MR indicated that many of these effects act independently of HDL-C, LDL-C and TG, the most frequently measured lipid fractions. Higher concentrations of TG, VLDL-C, Rem-Chol and Apo-B increased heart failure (HF) risk; often independently of LDL-C, HDL-C or TG. Finally, a subset of these exposures associated with non-CVD traits such as Alzheimer’s disease (AD: HDL-C, LDL-C, IDL-C, Apo-B), type 2 diabetes (T2DM: VLDL-C, IDL-C, LDL-C), and inflammatory bowel disease (IBD: LDL-C, IDL-C).ConclusionsThe cholesterol content of a wide range of lipoprotein and apolipoproteins associate with measures of atherosclerosis, blood pressure, CRP, and CHD, with a subset affecting HF, T2DM, AD and IBD risk. Many of the observed effects appear to act independently of LDL-C, HDL-C, and TG, supporting the targeting of lipid fractions beyond LDL-C for disease prevention.

Behavioral and metabolic responses of ponies fed a complete single diet, thermally processed and containing long fiber

• Processed and control diets were similar in the results for frequency of ‘abnormal’ behaviors • Complete and thermally processed diets are an alternative for horses’ nutrition in feed scarcity period. • Processed diets showed the greatest influence on glucose metabolism Alternatives horse's feed are ways to improve nutrient utilization as well as reduce production costs. The objective of this research was to describe behavioral and metabolic changes in ponies fed with complete diets composed of different grasses as fiber sources and industrial processing (thermal). Eight ponies were randomized into two Latin squares. The treatments were: ‘control diet’ (traditional management composed of 50% Tifton 85 hay and 50% pelletized concentrate supplied separately) and ‘complete diet’ (a single product composed of 50% of a fiber source [dehydrated whole corn plant, dehydrated elephant grass or dehydrated Tifton 85 grass] plus 50% concentrate) thermally processed (pelletized or extruded). The consumption of processed diets increased the frequency of 'standing' behavior (in an alert position) and reduced the frequency of 'feeding' behavior and the 'consumption time' (in minutes) of the diet (P < 0.05). There were no differences (P > 0.05) among processed diets and control diet for the frequency of ‘rest’ (lying in sternal or lateral decubitus), ‘abnormal’ (stereotypes), ‘water’ ingestion, ‘other’ behaviors and amount of water ingested. A larger area under the curve (AUC) of glucose and insulin (P < 0.05) for the processed diets and a lower concentration of total cholesterol (P < 0.05) for the pelleted diet was found. The glucose and insulin AUC was higher when the ponies received the pelleted diet. The dehydrated whole corn plant diet resulted in greater insulin AUC. The extruded diet containing Tifton 85 resulted in higher concentrations of triglycerides (33.0 ± 4.1 mg dL −1 ) and VLDLc (6.6 ± 0.8 mg dL −1 ), followed by the other diets. The use of complete thermally processed diets altered the behavior of stalled ponies but did not increase the frequency of ‘abnormal’ behaviors and did not impair metabolism. More research needs to be developed to assess the effects of long-term use of pelletized or extruded complete feed for horses.

Monocyte-to-HDL ratio and non-HDL cholesterol were predictors of septic shock in newborns

The association between lipoprotein levels and late-onset neonatal sepsis has shown controversial results. The aims are to assess lipid profile, cytokines, and Monocyte-to-HDL (M/H) ratio as diagnostic and prognostic markers for late-onset neonatal sepsis. This prospective study included49septic neonates and 17controls. Cholesterol (CT), Triglyceride (TG), Very-Low-Density (VLDLc), Low-Density (LDLc), and High-Density Lipoproteins (HDLc) were measured at admission (D0) and on days3,7 and10 to evaluate septic shock outcomes. Cytokines and monocytes were evaluated by flow cytometry. Septic newborns showed higher IL-6 and IL-8 at D0 and CT levels on D7 and on D10, which also presented higher TG, VLDLc and non-HDL cholesterol concentrations than controls. The septic shock group (n = 22) revealed a higher number of male subjects, CRP, IL-6, IL-8 and IL-10 levels, while lower TG, HDLc, monocyte numbers and M/H ratio at admission compared to the non-shock group (n = 27). M/H ratio and non-HDL cholesterol on D0 were risk factors for septic shock (OR = 0.70, 0.49‒0.99; OR = 0.96, 0.92‒0.99, respectively). Decreasing levels from D0 to D3 of CT (OR = 0.96, 0.93‒0.99), VLDLc (OR = 0.91, 0.85‒0.98), and non-HDL cholesterol (OR = 0.92, 0.87‒0.98) were also predictors of septic shock. Lower M/H ratios and non-HDL cholesterol at admission and decreasing levels of cholesterol, VLDLc and non-HDL cholesterol during a hospital stay are associated with the development of septic shock in newborns with late-onset neonatal sepsis.

Association of the lipoprotein lipase and Apolipoprotein C-II gene polymorphisms with risk of dyslipidemia in smokers and non-smokers male

ObjectiveDyslipidaemia is considered a metabolic abnormality andan important risk factor that leads to atherogenic cardiovascular diseases. Cigarette smoking is associated with dyslipidaemia. This study aimed to demonstrate whether lipoprotein lipase enzyme (LPL) and Apolipoprotein CII (APOCII) gene polymorphisms can be considered as independent genetic risk factors for dyslipidaemia among smokers with various smoking durations.MethodsA total of 185 males (90 smokers and 95 non-smokers)were included in this study, Lipid profiles were measured and DNA was isolated. The LPL-Hind III and APO CII-Ava II polymorphisms were determined using the polymerase reaction-restriction fragment length polymorphisms (RFLP) technique.ResultsFor the LPL-Hind IIIpolymorphism H+H+ genotype group, the triglycerides TG and very-low-density lipoprotein cholesterol VLDL-C concentrations were significantly higher and the high-density lipoprotein cholesterol HDL-C concentration was significantly lower than those of the H–H- genotype. ForAPO CII-Ava II polymorphisms, compared with those of the A2A2 genotype group, the total cholesterol TC, TG, low-density lipoprotein cholesterol LDL-C and VLDL-C concentrations were significantly increased in the A1A2 genotype group, while the HDL-C concentration was significantly decreased.ConclusionsThe study revealed that the H+H+ or H + H-genotype of the LPL-Hind III polymorphism and the A1A1or A1A2 genotype of the APOCII-Ava II polymorphism were at higher risk of developing dyslipidaemia compared to the H–H- genotype of the LPL-Hind III polymorphism and A2A2 genotype of the APOCII-Ava II polymorphism.

Antidyslipidemic Effect of Fenugreek Seeds Powder against Sodium Fluoride-Induced Dyslipidemia in Male Rabbits

Background: Although fluoride is everywhere in the environment, the major environmental sources of population exposure to elevated levels of fluoride are water, food, beverages, air, food supplements, and dental products. Fluoride is a well determined non-biodegradable and moderate pollutant, which at high levels causes serious health problems. Objectives: The present study aimed to evaluate the anti-dyslipidemia effect of fenugreek seeds powder against dyslipidemia induced by sodium fluoride in male rabbits. Materials and Methods: This study included twenty-four adult male rabbits, which were divided into 4 groups, 6 rabbits for each. Group I (control group): Animals were provided with tape water and fed with normal diet for 30 days. Group II (Fenugreek seeds powder group): Fenugreek seeds powder was given to rabbits in food at a dose of 10 g per kilogram of diet weight/kg of body weight/day for 30 Days. Group III (sodium fluoride group): Rabbits were injected intraperitoneally with sodium fluoride at a dose of 15mg/kg of body weight/day for 30 consecutive days. Group IV (Sodium fluoride/fenugreek co-administered group): Fenugreek seed flour was added at a rate of 10 g per kilogram of diet weight, and rabbits were injected with intraperitoneally with sodium fluoride at a dose of 15mg/kg of body weight/day for 30 consecutive days. After thirty days, blood samples were taken for biochemical analysis. Biochemical analyzes were performed to measure of the serum concentrations of cholesterol, triglycerides, HDL-C, LDL-C, and VLDL-C in all groups. Results: The results of the study showed that the treatment of male rabbits with sodium fluoride resulted in a significant increase (P&lt;0.01) in the serum concentrations of cholesterol, triglycerides, LDL-C, and VLDL-C, and a significant decrease was observed (P&lt;0.01) in serum HDL-C compared with the control group. In rabbits received fenugreek seeds powder only, the serum triglycerides and VLDL-C were significantly (P&lt;0.01) decrease and the serum HDL-C was significantly (P&lt;0.05) increase compared with the control group. A significant decrease (P&lt;0.01) was observed in serum cholesterol, triglycerides, LDL-C, and VLDL-C, and a significant increase was observed (P&lt;0.01) in serum HDL-C in rabbits treated with sodium fluoride and fenugreek seeds powder compared with the sodium fluoride group, but these measurements did not reach normal levels in the control group. Conclusion: The results showed that injection of rabbits with sodium fluoride led to serum dyslipidemia, and the injection with sodium fluoride and treatment of fenugreek seeds powder alleviate dyslipidemia induced by sodium fluoride. The use of fenugreek seeds powder by humans can be considered beneficial in the alleviation of dyslipidemia. It is recommended that humans exposed to sodium fluoride should be advised to take Fenugreek seeds powder as a rich source of antioxidant to prevent serum dyslipidemia induced by sodium fluoride. Further studies are necessary to elucidate exact mechanism of the antidyslipidemic effect of Fenugreek seeds powder and potential usefulness of Fenugreek seeds powder as a protective agent against sodium fluoride induced dyslipidemia in clinical trials.

Performance, Immune Responses, and Blood Biochemistry of Broiler Chickens Fed with Plant Growth Compound

Today, medicinal plants and prebiotics are known as growth stimulants and can have beneficial effects on health and performance. This study was conducted to evaluate the effects of plant growth promoters and a prebiotic (lactose) on growth performance, immune responses, and blood biochemical parameters in broiler chicks. Seven hundred and fifty Arian broiler chicks (mixed-sex) were allocated to six treatments and five replicates at one day of age. Three plant-based growth promoters (ASRI1, ASRI2, and commercial supplement (Optifeed)) and two dietary prebiotic levels (0 and 1 kg/ton) were evaluated in a completely randomized design with 3×2 factorial arrangements. The results showed that growth performance and humoral and cellular immunities did not differ among experimental treatments; therefore, the herbal compounds ASRI1 and ASRI2 can be used as growth promoters equivalent to the commercial products currently used in the broiler chicken industry. An interaction effect of growth promoter × prebiotic was detected for concentrations of calcium in the serum. Serum calcium concentrations of birds fed ASRI2 and 1 kg/ton prebiotic were greater than those of chickens receiving the commercial growth promoter. Serum triglycerides and VLDL-C concentrations were significantly lower in birds treated with ASRI1 growth promoter compared to those fed with a commercial growth promoter. In conclusion, there was no difference between ASRI1, ASRI2, and Optifeed growth promoters in their effectiveness as promoters of growth and immunity of broiler chicks.

RNA interference targeting apolipoprotein C-III with ARO-APOC3 in healthy volunteers mimics lipid and lipoprotein findings seen in subjects with inherited apolipoprotein C-III deficiency

Abstract Background Individuals with triglycerides (TGs) ≥1,000 mg/dL (11.1 mmol/L) are at increased risk of acute pancreatitis. Genetic studies indicate that individuals with apolipoprotein C-3 (APOC3) loss-of-function mutations have low TGs, reduced cardiovascular risk and no observed adverse phenotype. RNA interference (RNAi) with ARO-APOC3 has shown deep and durable knockdown (KD) of APOC3 after single doses in healthy volunteers (HVs, presented at AHA 2019) with good tolerability. We report here initial results using multiple doses of ARO-APOC3 to silence APOC3 expression in HVs. Methods ARO-APOC3 was administered subcutaneously to HVs on days 1 and 29 at doses of 10, 25 or 50 mg (n=4 per group). Measured parameters included plasma concentrations of APOC3, LDL-C, TGs, VLDL-C and HDL-C. Results All HVs have received both doses and follow-up for most parameters is available through week (wk) 14 (10 wks after second dose) for the 10 and 25 mg doses and through wk 10 for 50 mg. Mean nadir for APOC3 levels occurred at wk 3 for 10 mg (−73%) and remained similar at wk 10 (−66%), at wk 6 for 25 mg (−90%) with no change at wk 10 and at wk 2 for 50 mg (−94%) unchanged at wk 8. TGs fell faster in the 50 mg group (wk 1: 10 mg −41%; 25 mg −47%; 50 mg −72%). By wk 6 the 25 and 50 mg results were similar (−68% and −74%, respectively) and remained similar through wk 14. 10 mg was less active with a nadir of −56% and mean reductions between 42% and 56% post-nadir. VLDL-C values mirrored TGs. LDL-C reductions were more modest and did not manifest a dose response. Mean nadirs (−23–26%) occurred 4–6 wks after the first dose, again with minimal change through 10–14 wks of follow-up. Consistent with genetic studies, HDL-C increased to a maximum at approximately wk 8 (10 mg +42%, 25 mg +48%, 50 mg +84%). ARO-APOC3 was well tolerated without serious or severe adverse events or dropouts related to drug. The most common adverse events were mild injection site AEs and headache. Conclusions Genetic deficiency of APOC3 is associated with substantial reductions in TGs, VLDL-C and increases in HDL-C without an adverse phenotype. Using RNAi to selectively suppress APOC3 production mimics these lipid and lipoprotein effects, with a duration of at least 10 weeks following a second dose and with good tolerability over 16 wks using doses ranging from 10 to 50 mg. Investigation of optimal dosing regimen is ongoing, especially with respect to dosing interval. This therapeutic approach has potential for treating patients with chylomicronemia at risk of pancreatitis. Funding Acknowledgement Type of funding source: Private company. Main funding source(s): Arrowhead Pharmaceuticals

Role of angiopoietin-like protein 3 in sugar-induced dyslipidemia in rhesus macaques: suppression by fish oil or RNAi

Angiopoietin-like protein 3 (ANGPTL3) inhibits lipid clearance and is a promising target for managing cardiovascular disease. Here we investigated the effects of a high-sugar (high-fructose) diet on circulating ANGPTL3 concentrations in rhesus macaques. Plasma ANGPTL3 concentrations increased ∼30% to 40% after 1 and 3 months of a high-fructose diet (both P < 0.001 vs. baseline). During fructose-induced metabolic dysregulation, plasma ANGPTL3 concentrations were positively correlated with circulating indices of insulin resistance [assessed with fasting insulin and the homeostatic model assessment of insulin resistance (HOMA-IR)], hypertriglyceridemia, adiposity (assessed as leptin), and systemic inflammation [C-reactive peptide (CRP)] and negatively correlated with plasma levels of the insulin-sensitizing hormone adropin. Multiple regression analyses identified a strong association between circulating APOC3 and ANGPTL3 concentrations. Higher baseline plasma levels of both ANGPTL3 and APOC3 were associated with an increased risk for fructose-induced insulin resistance. Fish oil previously shown to prevent insulin resistance and hypertriglyceridemia in this model prevented increases of ANGPTL3 without affecting systemic inflammation (increased plasma CRP and interleukin-6 concentrations). ANGPTL3 RNAi lowered plasma concentrations of ANGPTL3, triglycerides (TGs), VLDL-C, APOC3, and APOE. These decreases were consistent with a reduced risk of atherosclerosis. In summary, dietary sugar-induced increases of circulating ANGPTL3 concentrations after metabolic dysregulation correlated positively with leptin levels, HOMA-IR, and dyslipidemia. Targeting ANGPTL3 expression with RNAi inhibited dyslipidemia by lowering plasma TGs, VLDL-C, APOC3, and APOE levels in rhesus macaques.

Modulation of Reverse Cholesterol Transport by Lycopene Is Associated with Its Protective Role Against Cigarette Smoke Induced COPD and Lung Carcinogenesis in Ferrets (OR05-02-19)

Abstract Objectives Reverse cholesterol transport (RCT), which mediates removal of excess cholesterol from peripheral tissues, is a key player in persistent lung inflammation, a common feature of chronic obstructive pulmonary disease (COPD) and lung cancer, after cigarette smoke (CS) exposure. We have previously shown that lycopene, a carotenoid naturally occurring in tomatoes and tomato products, inhibits tobacco carcinogen (NNK)/CS-induced COPD and preneoplastic lesions in lung of ferrets, but the mechanism is unknown. We hypothesize that the protective role of lycopene against NNK/CS-induced COPD and lung preneoplastic lesions is associated with restoring the RCT in ferrets. Methods Male ferrets (4 groups, n = 12−16/group) were exposed to combination of NNK and CS with or without lycopene feeding at low (LL) and high (HL) doses (equivalent to ∼30 mg and ∼90 mg lycopene/day in humans, respectively) for 22 weeks. Ferrets in NNK/CS groups were given NNK (50 mg/kg body weight, i.p. injection) once a month for 4 consecutive months, and were exposed to CS for 30 min/day for 22 weeks. The animals were fed lycopene or placebo starting three weeks prior to the NNK injections and continued until the end of the study. Results NNK/CS exposure significantly increased total cholesterol levels in both plasma and lungs of ferrets (P &lt; 0.05), which was associated with COPD and lung preneoplastic lesion development. In lungs, HL and LL doses lowered NNK/CS-induced total cholesterol, with HL dose reaching significance (P &lt; 0.05); this was accompanied with the decreased lung lesions. HL group had significantly higher mRNA expression of critical genes involved in RCT (LXRα, PPARα, ABCA1 and ABCG1) as compared to NNK/CS group (P &lt; 0.05). Additionally, HL feeding significantly increased protein levels of both nuclear hormone receptors, LXRα and PPARα, known regulators of ABCA1/G1 transporters (P &lt; 0.05). In plasma, lycopene restored total cholesterol and HDL-C concentrations to normal. Plasma triglyceride, LDL-C and VLDL-C concentrations were not significantly different between groups. Conclusions These data demonstrate an important association between lycopene protection against NNK/CS induced lung lesions and pulmonary cholesterol homeostasis through the restoration of the impaired RCT. Funding Sources NIH/NCI, USDA/ARS grants.

Biochemical effects of Pomegranate Grinded Peels on High Sucrose Stressed Rabbits

Pomegranate is a drought tolerant and long lived plant which grows popularly in arid and semiarid zones in the Mediterranean countries. Pomegranate was reported to have antimicrobial, anti-inflammatory, anti-tumor, anti-hepatotoxic, antiviral, anti-diabetic activities and they can improve oral, skin and cardiovascular health. Sucrose is a non-reducing disaccharide made up of 50% glucose and 50% fructose and has a moderately high glycemic index. High-sucrose diets have been used in animal models to induce well-characterized metabolic dysfunctions including cardiovascular risk factors, insulin resistance, dyslipidemia and hypertension. The present research aimed to evaluate the effects of pomegranate grinded peel extract supplementation on blood parameters of male rabbits stressed by high sucrose diet for 4 months, Through evaluation of serum total lipids, triacylglycerols, total cholesterol, HDL-c, LDL-c, VLDL-c and atherogenic indices, in addition to serum urea, creatinine, glucose, testosterone, zinc and copper concentrations. High sucrose supplementation induced a significant increase in Serum total lipids, triacylglycerols, total cholesterol, LDL-c, VLDL-c, atherogenic indices, glucose, testosterone and copper concentrations, in addition to non-significant increase in serum urea, creatinine concentrations. On contrast, it exhibited a significant decrease in serum HDL-C and zinc concentrations. Pomegranate peel powder supplementation was able to mitigate and ameliorate stress induced by high sucrose ration and showed pronounced curative effect against hyperlipidemia and deviated serum atherogenic indices as well as maintained glucose, testosterone copper and zinc toward control levels. The results of the present study suggested that pomegranate has the potential to exert curative effects against hyperlipidemia.

Oat beta-glucan as a dietary supplement for dogs.

The aim of this study was to evaluate the effects of oat beta-glucan supplementation on metabolic, physiological, immunological and nutritional variables in adult dogs. Fourteen dogs were fed a control diet or a diet supplemented with 1% beta-glucan during 71 days. Serum concentrations of glucose, total cholesterol and lipoprotein fractions, as well as plasma concentrations of peptide YY and ghrelin, were determined. In addition, coefficient of total tract apparent macronutrient digestibility (CTTAD), food intake and fecal output, score, and pH were evaluated. For evaluation of immunological variables, serum concentrations of interleukin-4 and interferon gamma were determined on days 0, 57 and 71, which corresponded to blood collection prior to beta-glucan supplementation, and at 7 and 14 days post first and second dose of vaccination (Pneumodog®, Merial, Campinas, Brazil), respectively. Animals fed the supplemented diet showed (P<0.05) lower serum concentrations of total cholesterol and low and very low density lipoproteins, lower coefficients of total tract apparent digestibility of dry matter, organic matter, mineral matter and ether extract, higher fecal output and lower fecal consistency, as well as a tendency (P = 0.07) of decreasing the coefficient of total tract apparent digestibility of crude protein. Moreover, the supplemented diet resulted in lower number of red blood cells, hematocrit percentage and hemoglobin concentration 21 days post-vaccination as well as lower serum concentration of interleukin-4 seven days post-vaccination (P<0.05). It is concluded that oat beta-glucan extract can be used as a dietary supplement for dogs at a dose of 10 g/kg of food, being effective in reducing blood concentrations of total cholesterol, LDL-c and VLDL-c as well as CTTAD of nutrients, demonstrating a potential to be used in the feeding of obese animals. In addition, by reducing the predominance of a Th2 response, oat beta-glucan can positively modulate the vaccine response of animals.

Abstract MP38: Effect of Dietary Carbohydrate Type on Serum Lipid Profile, Adipose Tissue Macrophage Infiltration and Inflammatory Status, and Peripheral Macrophage Cholesterol Efflux

Introduction: While much has been published on the effect of different amounts of dietary carbohydrate (carb) on cardiovascular disease (CVD) risk, data on effects of different types of carb (simple, refined and unrefined) are limited. Objective: To determine the relative comparability for an isocaloric replacement of unrefined-carb (UC; e.g., brown rice and bread/pasta made from whole wheat flour), refined-carb (RC; e.g., white rice and bread/pasta made from white flour) and simple-carb (SC; e.g., sucrose and high fructose corn syrup) on serum lipid and lipoprotein profile, adipose tissue gene expression of macrophage and inflammation markers, cytokine secretion and adipocyte size, and cholesterol efflux in peripheral blood mononuclear cells (PBMC). Methods: Study subjects (men and postmenopausal women [N=11], 65±8 y, BMI 29.8±3.2 kg/m 2 , LDL-C ≥2.6 mmol/L) were provided with isocaloric diets (60% E total carb, 15% E protein, 25% E fat) enriched in SC, RC or UC for 4.5 weeks, with a 2-week washout period between diet phases, using a randomized, cross-over design. Body weight was maintained within +/- 2 kg. At the end of each diet phase serum lipid and lipoprotein profile was determined using standard methods. Abdominal subcutaneous adipose tissue biopsies were collected and used to assess gene expression of macrophage and inflammation markers, cytokine secretion, and cell size. PBMCs were isolated and cultured to assess cholesterol efflux. Results: Fasting serum TC (p=0.004) and LDL-C (p=0.002) concentrations were higher after subjects consumed the RC diet than after the SC and UC diet phases. No significant differences were observed among diets in serum HDL-C (p=0.32), VLDL-C (p=0.17), TG (p=0.19), and NEFA (p=0.99) concentrations. Adipocyte size was largest after subjects consumed the RC diet and smallest after the SC diet (p&lt;0.0001). There were no significant differences among diets in gene expression of the macrophage markers CD14 (p=0.62) and CD68 (p=0.62), and the inflammatory markers adiponectin (p=0.66), leptin (p=0.42), and IL-6 (p=0.92), except for a trend towards higher serum amyloid-1 expression after the RC diet (p=0.059). Inflammatory cytokine secretion of adiponectin (p=0.52), leptin (p=0.89), and IL-6 (p=0.07) were not significantly different among diet phases. No significant differences were observed in macrophage cholesterol efflux among diets (p=0.18). Conclusions: Consuming the RC diet resulted in higher serum TC and LDL-C concentrations, and concurrently increased abdominal subcutaneous adipocyte size. Dietary carb type had no significant effect on markers of macrophage infiltration, inflammation or cytokine secretion in adipose tissue, or macrophage cholesterol efflux. Results from this study provide mechanistic support for the epidemiologic findings that increasing consumption of RC diet is positively associated with CVD risk.

Effects of Prosopis farcta fruit Hydroalcoholic Extract on Serum Concentrations of Glucose and Lipids in Insulin Resistance Model of Rats

Background and Objective: Prosopis farcta fruit (PFF) is known to have antioxidant activity. Antioxidants can reduce and prevent dyslipidemia and hyperglycemic in diabetic patients. This study was conducted to investigate the effect of PFF hydroalcoholic extract on some blood biochemical parameters in insulin resistance model of rats. Methods: In this experimental study, diabetes was induced by feeding the animals with fructose (12% w/v) and soybean (20% dry matter) for 6 weeks followed by a single intraperitoneal injection of streptozotocin (30 mg/kg). The animals were divided into four groups: 1 and 2) Healthy and diabetic controls; 3 and 4) healthy and diabetic rats receiving PFF (100 mg/kg body weight). The blood samples were collected and the serum concentrations of glucose, triglycerides, cholesterol, HDL-C, LDL-C and VLDL-C were investigated. Results: Streptozotocin increased serum levels of glucose, triglycerides, cholesterol and LDL-C (P < 0.05), but PFF extract lowered the serum concentrations of these variables (P < 0.05) in diabetic rats. Conclusions: PFF extract might be useful for the treatment of hyperglycemic and hyperlipidemic in diabetic patients.

Evaluation of Lipid Profile in Adolescents

Abstract Background: Atherosclerosis is a chronic, multifactorial and insidious disease that can begin in childhood and adolescence, and whose major consequences appear during adulthood. Serum levels of lipoproteins, such as LDL-c, total cholesterol (TC), HDL-c, and non-HDL-c can be used as a screening method for disease diagnosis. In Brazil, few studies have correlated the serum levels of those lipoproteins with age. Objective: To evaluate the serum concentrations of TC, LDL-c, HDL-c, VLDL-c, non-HDL-c and triglycerides (TG) of adolescents aged 10 to 19 years in the municipality of Araucaria, Parana state. Methods: Cross-sectional retrospective study, collecting the following data from 600 adolescents: age, sex and serum levels of TC, LDL-c, HDL-c and TG from June to December 2016. Data were analyzed using the SPSS software 2.0, with Mann-Whitney U test and Spearman coefficient of correlation to identify statistical significance (p < 0.05). Results: The female sex showed higher serum levels of TC, TG and LDL-c than the male sex. The HDL-c levels were identical in both sexes, with 48% of desirable values and 52% of low values. This study identified a strong correlation between the lipids and association with the age group of 10 to 14 years. Conclusion: Non-HDL-c showed stronger correlation with the other lipids (TG, LDL-c and TC) as compared to LDL-c, suggesting that non-HDL-c can be used as an effective complementary diagnostic method to assess the risks for atherosclerosis in adolescents.

Atherogenic Lipoprotein Subfractions and Carotid Atherosclerosis in Menopausal Women.

The aim of the study was to evaluate the relationship between cholesterol contained in very-low-density lipoproteins (VLDL-C), intermediate-density lipoproteins (IDL-C), low-density lipoproteins, high-density lipoproteins, and carotid intima-media thickness (cIMT) and carotid plaques in 228 postmenopausal women (63.1 ± 8.2 years) who participated in the ATENA Project and underwent clinical, biochemical (including the assay of lipoproteins using the Lipoprint system), and carotid ultrasound tests. Very-low-density lipoprotein cholesterol had a statistically significant linear association with cIMT ( P < .001), which remained significant after adjustment for age, smoking, systolic blood pressure, glucose, and body mass index ( r2 = .20, P < .05). Higher concentrations of IDL-C and cholesterol contained in triglyceride-rich lipoproteins (TRL-C, ie, VLDL-C + IDL-C) were associated with plaques in the common carotid (tertile III/tertile I: odds ratio [OR] = 2.52, 95% confidence interval [CI] = 1.21-5.32, P < .02; OR = 2.30, 95% CI = 1.05-5.01, P < .05, respectively), after adjustment for main cardiovascular risk factors. In conclusion, high concentrations of VLDL-C and TRL-C are independently associated with the presence of carotid plaques. Their assay represents a useful tool for improving our knowledge on the role of different classes of lipoproteins in atherosclerosis.