Viverricula Research Articles

Development of molecular sex identification method for Asian palm civet (Paradoxurus hermaphroditus) and Small Indian civet (Viverricula indica): a step towards forensic identification

Civet coffee and perfume are premium products in demand worldwide. Consequently, the civet population is threatened by several factors (e.g., the rapidly growing trend of civets being removed from the wild and held in captivity), raising concerns about civet welfare and conservation. In light of this, our aim was to develop a molecular sex identification method for the Asian palm civet (Paradoxurus Hermaphroditus) and the Small Indian civet (Viverricula Indica). We collected five Asian palm civet tissue samples (two male, one female, and two of unknown sex) and one female Small Indian civet from the repository, Advanced Institute for Wildlife Conservation (AIWC). DNA was extracted from all tissue samples using the DNeasy Blood and Tissue Kit. For sex identification, we optimized the PCR conditions to amplify partial fragments of sex-determining region Y (Sry) and the zinc finger X (Zfx) genes using DNA from two male Asian palm civet samples. The PCR amplicons were examined on a 2% agarose gel, and two male civets showed one distinct band at 250 bp (for Sry) and another at 120 bp (for Zfx). To confirm this finding, the PCR amplicons (Sry and Zfx genes) from one male sample were sequenced and % similarity and nucleotide pairwise analysis were calculated. The Sry and Zfx sequences showed 96.99% (for Sry) and 97.78% (Zfx) similarity to the masked palm civet, the only species in the families Paradoxurinae or Viverridae for which sequence data was publicly available. These results confirmed that the optimized PCR conditions are suitable for amplifying the Sry and Zfx genes in the Asian palm civet, which will be helpful for sex identification. Subsequently, we used this optimized method for the sex identification of three Asian palm civets and one Small Indian civet sample obtained from the repository, AIWC. Additionally, we used the method for identification of sex in a seized civet tissue sample received for forensic analysis. This report is the first to focus on developing a method for identifying the sex of Asian palm civets and Small Indian civets. This method will be helpful for researchers working on the ecology and conservation of civet population.

Lions & sea lions & bears, oh my: utilizing museum specimens to study the ossification sequence of carnivoran taxa

BackgroundMammalian skeletons are largely formed before birth. Heterochronic changes in skeletal formation can be investigated by comparing the order of ossification for different elements of the skeleton. Due to the challenge of collecting prenatal specimens in viviparous taxa, opportunistically collected museum specimens provide the best material for studying prenatal skeletal development across many mammalian species. Previous studies have investigated ossification sequence in a range of mammalian species, but little is known about the pattern of bone formation in Carnivora. Carnivorans have diverse ecologies, diets, and biomechanical specializations and are well-suited for investigating questions in evolutionary biology. Currently, developmental data on carnivorans is largely limited to domesticated species. To expand available data on carnivoran skeletal development, we used micro-computed tomography (micro-CT) to non-invasively evaluate the degree of ossification in all prenatal carnivoran specimens housed in the Harvard Museum of Comparative Zoology. By coding the presence or absence of bones in each specimen, we constructed ossification sequences for each species. Parsimov-based genetic inference (PGi) was then used to identify heterochronic shifts between carnivoran lineages and reconstruct the ancestral ossification sequence of Carnivora.ResultsWe used micro-CT to study prenatal ossification sequence in six carnivora species: Eumetopias jubatus (Steller sea lion, n = 6), Herpestes javanicus (small Indian mongoose, n = 1), Panthera leo (lion, n = 1), Urocyon cinereoargenteus (gray fox, n = 1), Ursus arctos arctos (Eurasian brown bear, n = 1), and Viverricula indica (small Indian civet, n = 5). Due to the relatively later stage of collection for the available specimens, few heterochronic shifts were identified. Ossification sequences of feliform species showed complete agreement with the domestic cat. In caniforms, the bear and fox ossification sequences largely matched the dog, but numerous heterochronic shifts were identified in the sea lion.ConclusionsWe use museum specimens to generate cranial and postcranial micro-CT data on six species split between the two major carnivoran clades: Caniformia and Feliformia. Our data suggest that the ossification sequence of domestic dogs and cats are likely good models for terrestrial caniforms and feliforms, respectively, but not pinnipeds.

Lack of detection of SARS-CoV-2 in wildlife from Kerala, India in 2020-21.

Spillover of SARS-CoV-2 into a variety of wild and domestic animals has been an ongoing feature of the human pandemic. The establishment of a new reservoir in white-tailed deer in North America and increasing divergence of the viruses circulating in them from those circulating in the human population has highlighted the ongoing risk this poses for global health. Some parts of the world have seen more intensive monitoring of wildlife species for SARS-CoV-2 and related coronaviruses but there are still very large gaps in geographical and species-specific information. This paper reports negative results for SARS-CoV-2 PCR based testing using a pan coronavirus end point RDRP PCR and a Sarbecovirus specific E gene qPCR on lung and or gut tissue from wildlife from the Indian State of Kerala. These animals included: 121 Rhinolophus rouxii (Rufous Horsehoe Bat), six Rhinolophus bedommei (Lesser Woolly Horseshoe Bat), 15 Rossettus leschenaultii (Fulvous Fruit Bat), 47 Macaca radiata (Bonnet macaques), 35 Paradoxurus hermaphroditus (Common Palm Civet), five Viverricula indica (Small Indian Civet), four Herpestes edwardsii (Common Mongoose), ten Panthera tigris (Bengal Tiger), eight Panthera pardus fusca (Indian Leopard), four Prionailurus bengalensis (Leopard cats), two Felis chaus (Jungle cats), two Cuon alpinus (Wild dogs) and one Melursus ursinus (sloth bear).

Distributional patterns and habitat association of sympatric carnivores in Margalla Hills National Park, Pakistan, and a comparison of conventional versus molecular identification in carnivore scatology

Abstract Knowledge of a species’ distribution is important for developing effective conservation programs. In Pakistan, little is known about the status or distribution of most carnivores coexisting in the same landscape. To address this knowledge gap, we studied distribution patterns of coexisting carnivores in Margalla Hills National Park, using both conventional as well as DNA-identified scats, and other signs. Although scat surveys remain a popular approach to study carnivores, scat identification based on morphology alone is error-prone. As part of our study, we therefore evaluated accuracy of morphological identification of scats using genetic techniques. Field surveys were conducted from September 2015 to December 2018. Using 593 direct (sighting, camera trapping, road kills) and indirect (scats, footprints) field signs, we detected total 11 carnivore species in the park, including 10 that were represented in the subset of 248 (47.7%) scats identified from DNA. The molecular analyses confirmed that the misidentification rate was highest for red fox (Vulpes vulpes) (8.47%), followed by Asiatic jackal (Canis aureus) (7.66%), but least for small Indian civet (Viverricula indica) (3.63%). For investigating habitat association of carnivores, and to test for the associations between species presence and Normalized Difference Vegetation Index (NDVI), we compared the mean and standard deviation of NDVI of each species’ presence locations with the mean and standard deviation of NDVI along the 23 sampling transects. Based on DNA-verified and all scats, carnivore species showed a range of mean NDVI, suggesting, preliminarily, some species may utilize a greater diversity of habitat types than others.

Roadkill records of two civet species on National Highway 715 passing through Kaziranga-Karbi Anglong landscape complex, Assam, India

This study was conducted on a continuous 64-km stretch of National Highway (NH) 715, which bifurcates the Kaziranga-Karbi Anglong landscape complex, with Kaziranga National Park on its southern side and North Karbi Anglong Wildlife Sanctuary on the northern side. The survey was carried out from October 2017 to September 2018 via two-way journeys three days a week, with two observers using a motor vehicle at a steady speed of 25–35 km/hour. Roadkill reports of one Large Indian Civet Viverra zibetha and six Small Indian Civets Viverricula indica were collected. Both the species are solitary and nocturnal, and prefer to inhabit secondary landscapes intermingled with human habitation. This stretch of NH 715 forms a crucial passage for wildlife foraging and breeding, and this study reflects the impacts of roads causing wildlife-vehicle collision for two civet species.

Assemblage, relative abundance and activity pattern of wild mammals in the human-dominated landscape of Vansda taluka, Gujarat, India

Abstract Conservation of mammals in human-dominated landscapes requires essential information on their ecological aspects. We used camera traps to assess the wild mammal assemblage, their relative abundance, and activity patterns in the human-dominated landscape of Vansda taluka, Gujarat, India. We deployed 80 camera traps in square grids of 2 km2 resulting in an area coverage of 160 km2. To assess the mammal assemblage, relative abundance of species, and activity pattern, respectively, we used a count of species, relative abundance index (RAI), and circular statistics. We captured 13 mammals, including the wild pig, Indian hare, leopard, rhesus macaque, common grey mongoose, small Indian civet, common palm civet, Indian crested porcupine, hanuman langur, chital, barking deer, striped hyena, and jungle cat. The wild pig was the most abundant (RAI = 10.29), while the jungle cat was the least abundant (RAI = 0.04) species. Hanuman langur, rhesus macaque, and chital showed diurnal activity, while striped hyena, barking deer, small Indian civet, common palm civet, and Indian crested porcupine showed nocturnal activity. Leopard, wild pig, common grey mongoose, and Indian hare showed cathemeral activity pattern. The present study serves as a baseline for future studies and help formulate conservation strategies for wild mammals in the study area.

Niche partitioning by sympatric civets in the Himalayan foothills of Pakistan.

Niche overlap between sympatric species can indicate the extent of interspecific competition. Sympatric competing species can exhibit spatial, temporal, and dietary adjustments to reduce competition. We investigated spatial, temporal, and dietary niche overlap of sympatric Asian palm civet (Paradoxurus hermaphroditus) and small Indian civet ( Viverricula indica), in and around Pir Lasura National Park, Pakistan. We used remote cameras to determine the frequency and timing of detections to estimate spatial and temporal overlap, and prey remains from scats to estimate dietary overlap. We collected scat samples of Asian palm civet (n = 108) and small Indian civet (n = 44) for dietary analysis. We found low spatial (Oij = 0.32) and temporal (Δ = 0.39) overlap, but high dietary niche overlap (0.9) between these two civet species. Both civet species were detected at only 11 camera sites and small Indian civets were detected most frequently during 2:00-5:00 h and 8:00-10:00 h, whereas Asian palm civets detections were greatest during 20:00-2:00 h. The overall niche breadth of Asian palm civet was slightly narrower (L = 9.69, Lst = 0.31) than that of the small Indian civet (L = 10, Lst = 0.52). We identified 27 dietary items (15 plant, 12 animal) from scats of Asian palm civet including Himalayan pear (Pyrus pashia; 27%), Indian gerbil (Tatera indica; 10%), Rhesus monkey (Macaca mulatta; 4%), and insects (5%). Scat analysis of small Indian civets revealed 17 prey items (eight plant, nine animal) including Himalayan pear (24%), domestic poultry (15%), Indian gerbil (11%), and house mouse (Mus musculus; 5%). Both civet species consumed fruits of cultivated orchard species. Spatial and temporal partitioning of landscapes containing diverse foods appears to facilitate coexistence between Asian palm civets and small Indian civets.

Nutrient intake of captive small Indian civet (Viverricula indica) in Chiang Mai Zoo and Demonstrated Farming Under Royal Project in Ban Dong Yen, Thailand

Here we examined the feed and nutrient intake of captive small Indian civets (Viverricula indica) housed at the Chiang Mai Zoo (CMZ) and Demonstrated Farming Under Royal Project (DYF) in Ban Dong Yen in Thailand. The feed type and intake was recorded for 4 males and 5 females at both CMZ and DYF. Feed samples were collected for chemical analysis and nutrient intake was calculated in terms of dry matter (DM) to compare CMZ and DYF. There were 11 and 5 feed types at CMZ and DYF, respectively. DM intake of small Indian civets at CMZ was significantly lower than at DYF (32.84 ± 0.852 % vs 38.90 ± 0.184 %; P<0.05). Crude protein, ether extract (crude fat), ash, Ca and P intake of civets at CMZ was significantly lower than the intake at DYF (P<0.05). Meanwhile, crude fiber and NFE (nitrogen free extract) intake of civets at CMZ was significantly higher than at DYF (P<0.05). The amount of wax released by civets at CMZ was significantly higher than those at DYF (2.14 ± 0.43 vs 1.49 ± 0.01 g/head/month) (P<0.05). Results of this study provide guidelines and references for feeds and feed intake for captive small Indian civets.

Diel activity, habitat utilization, and response to anthropogenic interference of small Indian civets (<i>Viverricula indica</i>) in Shanghai urban areas based on camera trapping

Diel activity, habitat utilization, and response to anthropogenic interference of small Indian civets (<i>Viverricula indica</i>) in Shanghai urban areas based on camera trapping

Prey selection by the Indian tiger (Panthera tigris tigris) outside protected areas in Indias Western Ghats: implications for conservation

As habitat loss and fragmentation increase globally, biodiversity conservation approaches are increasingly being implemented at the landscape scale to maintain metapopulations of endangered species. The present study investigated the food habits of tiger Panthera tigris in the less protected reserve forests of Begur Range, a wildlife corridor in the Malenad–Mysore Tiger Landscape (MMTL) in India's Western Ghats. Fifty scat samples were collected and analyzed to determine the diet composition. The study recorded seven tiger prey species from the study area. The percentage composition of different prey species revealed that chital (Axis axis) constitutes 63.15% of the overall diet, whereas sambar (Rusa unicolor) and gaur (Bos gaurus) constituted 26.33% each. Contrary to our prediction, the relative biomass of different prey species showed that chital, sambar, and gaur constituted 95% of the overall diet. The presence of small prey species such as the Indian crested porcupine (Hystrix india) and small Indian civet (Viverricula indica) indicates opportunistic hunting behaviour and diverse prey selection by tigers. This study highlights the significance of areas outside the protected area network for conserving large carnivores and ecosystem processes associated with them in the Western Ghats global biodiversity hotspot.

Road network is a Death Trap for Wildlife – a note on the roadkill of Small Indian Civet (Viverricula indica) from the Banaskatha district of Gujarat, India.

Road network is a Death Trap for Wildlife – a note on the roadkill of Small Indian Civet (Viverricula indica) from the Banaskatha district of Gujarat, India.

Study of different indices of scapula of certain non-primate mammals in relation to locomotion

The movement of the forelimb chiefly depends on structure and function of the shoulder region. The functional morphology of the primate forelimb has been studied in detail but the non primate shoulder has not been explored to such extent. The present study was conducted on scapula bones of different (non-primate) mammals, including ox, horse, dog, rhinoceros, sloth bear, chital deer, barasingha, striped hyena and small Indian civet with regard to scapular indices.The infraspinous index was highest in sloth bear for the purpose of climbing. Superior angle of scapula bone was rounded in dog, striped hyena, small Indian civet and sloth bear, whereas in case of barasingha, horse, ox, rhinoceros and chital deer, was not rounded. It can be concluded that the scapula of barasingha, horse, dog, ox, rhinoceros are better adapted for running purposes only, whereas sloth bear and small Indian civet are better adapted for climbing, digging and various other manipulations.

The diel activity pattern of small carnivores of Western Ghats, India: a case study at Nelliampathies in Kerala, India

The diel activity pattern of small carnivores was studied using the camera trap technique at Nelliampathy Reserve Forest, Kerala, India. Six species of small carnivores were recorded during the study. These include Brown Palm Civet Paradoxurus jerdoni, Small Indian Civet Viverricula indica, Stripe-necked Mongoose Herpestes vitticollis, Brown Mongoose Herpestes fuscus, Nilgiri Marten Martes gwatkinsii, and Leopard Cat Prionailurus bengalensis. The maximum diel activity overlap was detected between the Brown Palm Civet and Small Indian Civet, while the activity overlap was minimal between the Stripe-necked Mongoose and Small Indian Civet.

Abundance, diversity and daily activity of terrestrial mammal and bird species in disturbed and undisturbed limestone habitats using camera trapping, Central Thailand

Abstract: Pla-ard M, Hoonheang W, Kaewdee B, Panganta T, Charaspet K, Khoiesri N, Paansri P, Kanka P, Chanachai Y, Thongbanthum J, Bangthong P, Sukmasuang R. 2021. Abundance, diversity and daily activity of terrestrial mammal and bird species in disturbed and undisturbed limestone habitats using camera trapping, Central Thailand. Biodiversitas 22: 3620-3631. This study on the abundance, diversity and daily activity of terrestrial mammal and bird species was conducted in the limestone mountainous area of Central Thailand, located on the east of Dong Phaya Yen-Khao Yai forest complex. Camera traps were placed in both habitats disturbed by limestone mining and undisturbed habitat areas. From the study, a total of 38 species of mammals and birds from 27 families in 13 orders were recorded, including 15 species of mammals from 6 orders, 12 families and 23 species of birds from 14 families in 7 orders. Fifteen species of mammals were recorded in the undisturbed area and 11 were recorded in the disturbed area, with the Malayan Pangolin, Small Indian Civet and Grey-bellied Squirrel found in the undisturbed area. However, the number of bird species in the limestone mining area was larger than in the undisturbed area. It was also found that there was no difference in the overall abundance and diversity of mammalian species between disturbed and undisturbed areas, which is not in accordance with the hypothesis. But in the case of wild birds, the relative abundance of wild birds was found to differ significantly between areas. A high number was found in the areas with mining activities, although there was no difference in the diversity index of the two areas. However, it was found that when the combined data was analyzed, there was a significant difference in the daily activity of both mammals and wild birds in both areas. Many rare wildlife species were recorded during this study, for example, the Malayan Pangolin, Serow, Northern Pig-tailed Macaque, Rufous Limestone-babbler, Golden Jackal, Leopard Cat, Large-toothed Ferret Badger, Small Asian Mongoose, Common Palm Civet, Small Indian Civet, Malayan Porcupine. The key measure proposed is to preserve some natural habitats within the areas with mining activities, as wildlife remains in the area.

Does the Presence of Shade Trees and Distance to the Forest Affect Detection Rates of Terrestrial Vertebrates in Coffee Home Gardens?

Complex agroforestry systems can host similar biodiversity levels to adjacent continuous forests and can offer important ecosystem services for wildlife. Species inhabiting adjacent forests, as well as species that prefer agroforestry systems, can benefit from this habitat matrix. It is necessary, however, to understand the species-specific adaptability to such a complex matrix. Indonesia is a biodiversity hotspot and hosts many endemic species that are threatened with extinction. Its human population relies heavily on agriculture, meaning that finding a balance between crop productivity and biodiversity is key for the long-term sustainability of local communities and wildlife. We aim to determine the influence of the presence of shade trees and distance to the forest on the detection rates of wildlife in coffee home gardens. In West Java, Indonesia, we monitored 23 gardens between April 2018 and March 2021 via camera traps, totalling 3856 days of monitoring in shade-grown and 3338 days in sun-exposed gardens. We also collected data in the nearby montane rainforest, totalling 1183 days of monitoring. We used Generalized Additive Models to estimate the influence of shade cover and distance to the forest on the detection rates of wildlife. The Sunda leopard cat Prionailurus javanensis was found more frequently in shade-grown gardens and used both the forest and agroforest matrix. Wild boars Sus scrofa mostly occurred in gardens adjacent to the forest, while barred buttonquails Turnix suscitator were associated with gardens far (>1 km) from the forest. Several species (civets Viverricula indica and Paradoxus musangus javanicus, Horsfield’s treeshrew Tupaia javanica, Javan ferret badger Melogale orientalis, Javan mongoose Herpestes javanicus) were not influenced by shade cover and distance to the forest, suggesting they are well adapted to the agroforestry system. Still, species of high conservation importance, such as Javan leopard Panthera pardus melas, Sunda porcupine Hystrix javanica, and grizzled langur Presbytis comata, were present in the forest but not in the agroforest, suggesting that the replacement of the forest by the agroforestry matrix is still detrimental. Nevertheless, it is important to maintain the complexity of the agroforestry system and connectivity with the neighbouring continuous forest to favour the long-term sustainability of this environment and the conservation of endemic species.

Biometrical analysis of scapula bone of fishing cat, leopard cat and small indian civet

Biometrical analysis of scapula bone of fishing cat, leopard cat and small indian civet

Application of Multi-Species Occupancy Modeling to assess mammal diversity in northeast Bangladesh

Bangladesh is one of the most densely populated countries on earth with 1033 persons/km2, yet it also harbors a high level of mammal diversity. Unfortunately, general ecological knowledge is limited in many areas of the country, leading to a lack of cohesive conservation initiatives. As a result, the country is quickly losing integral wildlife habitat. Here we assess the presence and community composition of mammal species in Northeastern Bangladesh. Between May 2014 and January 2015 we conducted camera trapping in four Reserve Forests in Northeastern Bangladesh: Atora Hill Reserve Forest (Atora Hill), Patharia Hill Reserve Forest (Patharia Hill), Rajkandi Reserve Forest (Rajkandi), and Tarap Hill Reserve Forest (Tarap Hill); for a combined total of 1283 trap nights across the four reserves. We then used a Multi-Species Occupancy Model (MSOM) to estimate mammal species occupancy, richness, and community composition in Northeastern Bangladesh. We recorded 27 species of mammals, 23 of which were non-domesticated mammals, consisting of six orders and 15 taxonomic families. Our model suggests there are potentially 37 species of mammals occur in the Northeast Bangladesh. MSOM model also suggests occupancy for each species varies in the region from 69 percent for wild boar (Sus scrofa) to 0.1 percent for small Indian Civet (Viverricula indica). Our study provides the baseline for mammal species richness and community composition in Northeastern Bangladesh. Based on our findings we recommend that public-private partnerships, targeted protected areas, and increased capacity of the local people, are integral to facilitate coexistence between humans and wildlife and to aid in the conservation of mammal diversity in Northeastern Bangladesh.

Carnivore Protoparvovirus-1 Associated With an Outbreak of Hemorrhagic Gastroenteritis in Small Indian Civets.

Carnivore protoparvovirus-1 (CPPV-1) infection has been reported frequently in both domestic and wildlife species including wild carnivores. Fifty-five captive small Indian civets (Viverricula indica), farmed for perfume production in Eastern Thailand, showed clinical signs of acute bloody diarrhea, anorexia, vomiting, circling, and seizures. The disease spread within the farm and resulted in the death of 38 of the 55 civets (69% mortality) within a month. Fecal swabs were collected from the 17 surviving civets, and necropsy was performed on 7 of the dead civets. Pathologic findings were severe hemorrhagic gastroenteritis with generalized lymphadenopathy. CPPV-1 was identified in both fecal swabs and postmortem samples by species-specific polymerase chain reaction. Further whole-gene sequencing and restriction fragment length polymorphism analysis suggested feline panleukopenia virus (FPV) as the causative agent. The viral tropism and tissue distribution were confirmed by immunohistochemistry, with immunolabeling in the cytoplasm and nucleus of small intestinal crypt epithelial cells, villous enterocytes, histiocytes in lymphoid tissues, myenteric nerve plexuses, and cerebral and cerebellar neurons. Phylogenetic analysis of civet-derived CPPV-1 indicated a genetic similarity close to the FPV HH-1/86 strain detected in a jaguar (Panthera onca) in China. To our knowledge, this mass die-off of civets is the first evidence of disease associated with CPPV-1 infection in the subfamily Viverrinae. These findings support the multi-host range of parvovirus infection and raises awareness for CPPV-1 disease outbreaks in wildlife species.

Diversity, abundance, activity period, and factors affecting the appearance of wildlife around the corridors between Khao Yai- Thap Lan National Parks, Thailand by camera trapping

Abstract. Sukmasuang R, Charaspet K, Panganta T, Pla-ard M, Khioesree N, Thongbanthum J. 2020. Diversity, abundance, activity period, and factors affecting the appearance of wildlife around the corridors between Khao Yai-Thap Lan National Parks, Thailand by camera trapping. Biodiversitas 21: xxxx. The study on diversity, abundance, activity period, and factors affecting the appearance of wildlife around the wildlife corridors was investigated using camera trap method from September 2017 to November 2018. The corridors located at the royal highway in Ban Bu Phram Subdistrict, Na Di District, Prachinburi Province, Thailand. Based on 21 camera locations, 3,172 trap nights and 6,707 captured pictures totally resulted that a total of 37 species represented by 14 orders and 26 families consisting of 13 species of herbivores, 10 species of carnivores, 10 species of aves and 4 species of reptiles were photographically recorded in the area. Among the wildlife species, 4 species were listed as endangered according to IUCN Red List including Malayan pangolin, large-spotted civet, dhole, Asian forest tortoise, 5 species were listed as vulnerable, there were Sumatran serow, gaur, sambar deer, northern pig-tailed macaque, Asian black bear, and 21 species were listed as least concerned, such as lesser oriental chevrotain, Himalayan porcupine, Asian palm civet, large Indian civet, small Indian civet, hog badger, small Asian mongoose, golden jackal, red jungle fowl, Siamese fireback, coral-billed ground cuckoo and so on. Based on photographic rate of each species, the herbivorous wildlife species represented the relative abundance index of 15.04. The carnivorous wildlife species which had the relative abundance index of 4.70, while the RAI of the aves and reptiles were 1.91 and 0.12. The activity period found that the majority were found at night. This study showed that wildlife corridors have a high influence on the appearance of wildlife, demonstrating the potential of the corridors be used by wildlife for travelling. However, the wildlife corridors should be designed to be suitable for the particular types of animals, especially by increasing the construction of underpasses for mammal at locations with the highest and the number of crossings and also must reduce noise from vehicles, especially at night.

Exploring and interpreting spatiotemporal interactions between native and invasive carnivores across a gradient of rainforest degradation

Studies of elusive carnivores often rely on passive sampling when investigating either spatial or temporal interactions. However, inference on behavioral mechanisms are usually lacking. We present an analysis that combines previously published spatial co-occurrence estimates and temporal kernel density estimates to explore spatiotemporal interspecific interactions. We do so by deriving a spatiotemporal value (STV) that is a relative measure of potential interaction in both niche dimensions, across a gradient of degradation, for rainforest carnivore pairs in Madagascar. We also use a conceptual framework to provide insight into the potential behavioral mechanisms of habitat selection. Of the six native and three invasive carnivores, we estimate the spatiotemporal interactions for twelve pairings, which range from no spatial/temporal relationship (n = 5) to spatiotemporal aggregation or segregation (n = 7). We visualized these spatiotemporal interactions along a fragmentation gradient and demonstrate that these interactions are not static, as STV overlap increases with increasing anthropogenic disturbance. Of the three invasive carnivores (free-ranging dogs Canis familiaris, cats Felis species, and small Indian civets Viverricula indica) the latter had the highest number of spatial occurrence (n = 4) and spatiotemporal overlap (n = 4) relationships with native carnivores. Our results highlight the potential for increasing direct and indirect interactions between native and invasive species as forest degradation and invasive predators increase. Our approach allows us to better understand adaptive behaviors, plasticity in temporal activity, community assemblage, and to develop targeted conservation strategies to manage ecological communities in rapidly changing ecosystems.