Aim. To study the levels of proangiogenic and antiangiogenic growth factors in vitreous fluid in choroidal melanoma by multiplex flow cytometry. Methods. The concentration of proangiogenic (IL-8, angiogenin, TNFα, VEGF, bFGF) and antiangiogenic (IFNα, TGFβ, IFNγ) growth factors in the vitreous fluid of 5 patients with choroidal melanoma (MC) was determined by multiplex flow cytometry. Thirty-eight patients with senile cataracts served as a matching group. Results. Compared with senile cataract, in the vitreous fluid of the eyes with choroidal melanoma, there was a significant increase in the frequency of detection and level of proangiogenic cytokines TNFα (80,0 % vs. 47.5 %, p<0.05; Msr±m: 4.3±1.1 pg/ ml against 1.4±0.3 pg/ml, p<0.05), IL-8 (100 % vs. 75 %, p<0.01; 323.2±227.9 pg/ml versus 8.5±1.5 pg/ml, r<0.001), angiogenin (11704.9±1767.7 pg/ml versus 2820.15±1404.9 pg/ml, r<0.01), VEGF (100,0 % vs. 68.2 %; p<0.05; 471.49±154.6 pg/ml vs. 18.4±3.2 pg/ml, p<0.05; 471.49±154.6 pg/ml vs. 18.4±3.2 pg/ml, p<0.05) and FGFb (60 % vs. 26.7 %, p<0.05; Msr: 44.6±16.2 vs. 2.7±1.0, p<0.001). In both groups of patients, TGFβ antiangiogenic factor was not detected, but the concentration of IFNγ was found in five of the eight samples at the level of 14.9±12.2 pg / ml, and the levels of IFN were 4-fold higher: 17.6±3.9 pg / ml against 4.4±0.4 pg / ml (p<0.05). Conclusions. Elevated levels of proangiogenic growth factors (IL-8, angiogenin, TNFα, VEGF and bFGF) in the vitreous fluid in choroidal melanoma indicate simultaneous presence of three mechanisms for stimulating angiogenesis, two of which are independent of VEGF, act independently and may show synergism. Insufficiently high levels of interferons (IFNγ and IFNa) against the background of TGFβ absence of in the vitreous fluid suggests that secretion and control of regulation of the natural angiostatic link of angiogenesis in the eyes with choroidal melanoma is suppressed. High levels of cytokines with pluripotent (proangiogenic and proinflammatory) properties indicate that in choroidal tumors inflammation may play the role of angiogenesis promoter. Key words: choroidal melanoma; growth factors; vitreous fluid; angiogenesis; antiangiogenic therapy.