B Complex Research Articles

Effect of lowering blood cortisol level along with progesterone priming on intensifying the secondary symptoms of oestrus in Murrah buffaloes during the hot summer months.

The present study sought to intensify oestrus symptoms in heat-stressed pre-pubertal Murrah buffalo heifers. The first experiment aimed at lowering the blood cortisol level. Twenty pre-pubertal buffalo heifers approximately 36-40months of age were randomly allocated to four groups of five buffaloes. Group Gly received 100 ml glycerol orally for 8 days (day 0 is the start of treatments), group Gly-E received intra-muscular injections of 500 mg tocopherol (vitamin E activity) and 150 mg sodium selenite on alternate days (four treatments) in addition to treatments received by group Gly, group Gly-E + B-Ch received intra-muscular injections of vitamin B-complex and choline daily for 8 days in addition to the treatments received by group Gly-E. The control group Co-1 did not received any treatments. Blood cortisol and glucose level was measured on day 7 post-treatment. The second experiment sought to test the effect of lowering the blood cortisol level and progesterone priming on the intensity of oestrus symptoms in heat-stressed pre-pubertal buffalo heifers. Eighty buffaloes were allocated into four groups of twenty buffaloes. P4 group received intra-vaginal progesterone CIDR for 5 days (day 0 is the day of start of treatment). Gly-E group received the same treatment as in the first experiment for 7 days (day 0-6) and P4 + Gly-E received the combination of P4 and Gly-E groups. The control group Co-2 did not receive any treatments; however, on day 6, all groups received intra-muscular injection of 2mg estradiol benzoate. The buffaloes were graded for oestrus symptoms on the basis of mucus discharge (A-scanty and B-copious), vulvar redness (slightly pink and moist-A and deep red and moist-B), ferning (nil or poor-A and good with arborization-B) and cellularity of cervical mucus (Type A goblet cells with round nucleus and abundant cytoplasm and Type B goblet cells with cylindrical nucleus and scanty cytoplasm). The blood glucose level (mean ± SEM) of Gly group on day 7 of treatment in experiment 1 was 58.3 ± 0.4mg/dl that was significantly greater than Co-1 (52.4 ± 0.4mg/dl), Gly-E (53.5 ± 0.8mg/dl) and Gly-E + B-Ch (52.7 ± 0.6mg/dl) groups; however, the difference was found to be non-significant among Co-1, Gly-E and Gly-E + B-Ch groups. The cortisol level (mean ± SEM) on day 7 in groups Gly (11.4 ± 0.6ng/ml), Gly-E (8.1 ± 0.5ng/ml) and Gly-E + B-Ch (7.5 ± 0.4ng/ml) was significantly lower than groups Co-1 (15.5 ± 0.3ng/ml), though the difference between groups Gly-E and Gly-E + B-Ch was non-significant and the level in group Gly-E significantly lower than Gly. Thus, the combination of vitamin E and selenium along with glycerol had the best effect in the reduction of blood cortisol level in heat-stressed buffaloes. In the second experiment the blood estradiol level on day 7 in all groups was around 40 pg/ml. Cortisol reduction (group Gly-E) improved oestrus symptoms (mucus discharge and ferning) compared to control, but when compared to group P4 showed non-significant difference with respect to mucus discharge, vulvar redness and ferning of cervical mucus. However, when cortisol reduction treatments were combined with progesterone priming (group P4 + Gly-E) oestrus symptoms were improved with respect to mucus discharge and ferning thus, combination treatment had synergistic effect. This effect was also observed with cellularity of cervical mucus, where combination treatment group P4 + Gly-E produced cervical mucus with significantly lower percentage of Type B cells compared to group P4 and group Gly-E. Thus, administration glycerol and vitamin E selenium combination reduces blood cortisol level and along with progesterone priming can be effective to improve the oestrus symptoms of buffaloes during the hot summer months.

Spastic Paraplegia and Ataxia in a Welder.

A 51-year-old welder (nonsmoker) developed subacute lower paresthesias and progressive spastic paraplegia and ataxia. Symptoms appeared 10 days after the first zinc exposure in the yacht construction site (8-hour day/40 days in a confined space with face shield only). Examination revealed loss of vibratory sensation, spasticity, hyperreflexia, and sensory ataxia. Serum heavy metals (Cu 1 mg/L and Pb 4,22 μg/L), B-complex vitamins, and paraneoplastics on serum and CSF were unremarkable. The elevated zinc level (137 μg/dL) was found. Spinal MRI showed combined degeneration (Figure). Myelopathy in zinc smelter workers was reported in the 19th century1 and recently rediscovered in excessive zinc ingestion. Copper secondary deficiency is hypothesized even if the normal copper serum level is reported.2 Two months later, copper became low (0.8 mg/L), a time-dependent process might explain late copper deficiency in our case. Symptoms ameliorated 4 months later with normalization of serum zinc/copper levels.

Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties.

Probiotics are health-beneficial microorganisms with mainly immunomodulatory and anti-inflammatory properties. Lactobacillus delbrueckii species is a common bacteria used in the dairy industry, and their benefits to hosting health have been reported. This study analyzed the core genome of nine strains of L. delbrueckii species with documented probiotic properties, focusing on genes related to their host health benefits. For this, a combined methodology including several software and databases (BPGA, SPAAN, BAGEL4, BioCyc, KEEG, and InterSPPI) was used to predict the most important characteristics related to L. delbrueckii strains probiose. Comparative genomics analyses revealed that L. delbrueckii probiotic strains shared essential genes related to acid and bile stress response and antimicrobial activity. Other standard features shared by these strains are surface layer proteins and extracellular proteins-encoding genes, with high adhesion profiles that interacted with human proteins of the inflammatory signaling pathways (TLR2/4-MAPK, TLR2/4-NF-κB, and NOD-like receptors). Among these, the PrtB serine protease appears to be a strong candidate responsible for the anti-inflammatory properties reported for these strains. Furthermore, genes with high proteolytic and metabolic activity able to produce beneficial metabolites, such as acetate, bioactive peptides, and B-complex vitamins were also identified. These findings suggest that these proteins can be essential in biological mechanisms related to probiotics’ beneficial effects of these strains in the host.

Consumption of Iron Rich Food, Supplement Intake and Prevalence of Anaemia Among Pregnant Women Attending Antenatal Clinical in Selected Hospitals in Enugu State

ObjectivesIncrease prevalence of anaemia in pregnancy in spite consumption of iron rich foods and micronutrient supplementation is worrisome. Hence, this study investigated the consumption of iron rich food, supplement intake and prevalence of anaemia among pregnant women attending antenatal clinic in selected hospitals in Enugu State.MethodsThe study employed a cross-sectional study design with multistage random selection of 449 pregnant women (PW) attending antenatal clinic in health facilities in Enugu State, Nigeria. Data were collected using a structured questionnaire and biochemical test for Haemoglobim level using a standard procedure. Data was analyzed using SPSS 23. Statistical analysis was done using Chi-square and Pearson correlation to establish the relationship between variables. A p-value < 0.05 was considered as the level of significance.ResultsThe result shows that about 42.1% of the pregnant women were between the age group 26–35years and 30.4% earned < N20,000 ($50) monthly. Less than half of the PW consumed vegetable, fruits, beef and fish ≥ 4 times weekly. Less than half took folic acid, B-complex vitamins and ferrous sulphate twice daily, 36.9% had Anaemia (27.8% mild; 5.3% moderate; and 3.8% severe anaemia) and 76.8% had malaria. There was significant relationships between iron rich consumption, supplement intake especially iron supplement and prevalence of anaemia among pregnant women attending antenatal clinic in this study (P < 0.05).ConclusionsThe study revealed poor consumption of iron rich food and poor compliance to supplements intake among pregnant women which accounted for the high prevalence of anaemia in the study population.Funding SourcesThe research is self funded.

Association of the B-complex vitamins with osteoporosis and osteoporotic fractures

Association of the B-complex vitamins with osteoporosis and osteoporotic fractures

0166 The Role of Vitamin B12 Supplementation in the Association between Depression Symptoms and Daytime Sleepiness

Abstract Introduction A substantial body of literature has demonstrated an association between depression and sleep disturbances. While depressive symptoms are strongly associated with limited sleep duration and quality, high depressive symptomology has been also linked to symptoms of hypersomnolence. Vitamin B12 supplementation is associated with reduced risk of depression and the enhancement of pharmacological treatment for depression, but less is known about the role of vitamin B12 on sleep disturbances associated with depressive symptoms. Thus, our current study examined vitamin B12 intake as a moderator of depression and daytime sleepiness in a national sample of adults. Methods The sample consisted of 5,553 adults who completed the 2017-18 National Health and Nutrition Examination Survey (M age=49.8, SD=18.6; 51.7% female). Participants reported on prescription and nonprescription dietary supplement use in the last 30 days, sleep habits and disorders adapted from the Munich Chronotype Questionnaire, and completed the Patient Health Questionnaire-9 for depression screening. All measures were administered by trained interviewers. A moderation analysis was performed using R statistical programming. The analysis controlled for age, gender, ethnicity, sleep duration, and other B-complex vitamins (e.g., B1, B2, B3, and B6). Results Depressive symptoms were significantly associated with greater daytime sleepiness (b=.06, p &amp;lt;.001). Furthermore, there was a significant interaction between depressive symptoms and vitamin B12 consumption (b=-.002, p=.003, R2=.12). Higher vitamin B12 consumption buffered the relationship between depressive symptoms and daytime sleepiness. In contrast, a stronger positive relationship between depressive symptoms and daytime sleepiness was observed among participants with lower Vitamin B12 consumption. Thus, the findings suggest that consuming vitamin B12 may be beneficial for counteracting daytime sleepiness associated with depression. Conclusion Findings from the current study suggest that vitamin B12 supplementation provides a small, but significant buffering effect on the relationship between depressive symptoms and daytime sleepiness. Although existing pharmacological and behavioral interventions for sleep and depression are clinically effective, vitamin B12 intake may be an additional modifiable behavior that could increase prognosis of treatment. Given the very modest interaction effect, further Support (If Any)

Effect of Baking and Grilling on B Vitamins of Selected Fishes and Chicken Parts

ABSTRACT Meats are considered a good source of B-complex vitamins. This investigation aimed to detect vitamins B1, B2, and B3 content in selected fishes and chicken parts using two different cooking techniques, baking and grilling. HPLC was used to detect vitamin B1, B2, and B3 concentrations in each raw and cooked meat. The vitamin B1, B2, and B3 amount of all samples decreased significantly after grilling and baking (p < .05). The average cooking loss of vitamin B1, B2, total vitamin B3, nicotinic acid, and nicotinamide in meat samples by grilling was 45%, 38%, 46%, 70%, and 45%, respectively. By baking, the average cooking loss was 52%, 57%, 55%, 66%, and 54%, respectively. The meat samples had remarkably lower nicotinic acid levels than nicotinamide. The highest cooking losses were seen in the nicotinic acid. Vitamins were more stable when fish and meats were cooked with grilling than with baking.

Functional and Phylogenetic Characterization of Bacteria in Bovine Rumen Using Fractionation of Ruminal Fluid.

Cattle productivity depends on our ability to fully understand and manipulate the fermentation process of plant material that occurs in the bovine rumen, which ultimately leads to the improvement of animal health and increased productivity with a reduction in environmental impact. An essential step in this direction is the phylogenetic and functional characterization of the microbial species composing the ruminal microbiota. To address this challenge, we separated a ruminal fluid sample by size and density using a sucrose density gradient. We used the full sample and the smallest fraction (5%), allowing the enrichment of bacteria, to assemble metagenome-assembled genomes (MAGs). We obtained a total of 16 bacterial genomes, 15 of these enriched in the smallest fraction of the gradient. According to the recently proposed Genome Taxonomy Database (GTDB) taxonomy, these MAGs belong to Bacteroidota, Firmicutes_A, Firmicutes, Proteobacteria, and Spirochaetota phyla. Fifteen MAGs were novel at the species level and four at the genus level. The functional characterization of these MAGs suggests differences from what is currently known from the genomic potential of well-characterized members from this complex environment. Species of the phyla Bacteroidota and Spirochaetota show the potential for hydrolysis of complex polysaccharides in the plant cell wall and toward the production of B-complex vitamins and protein degradation in the rumen. Conversely, the MAGs belonging to Firmicutes and Alphaproteobacteria showed a reduction in several metabolic pathways; however, they have genes for lactate fermentation and the presence of hydrolases and esterases related to chitin degradation. Our results demonstrate that the separation of the rumen microbial community by size and density reduced the complexity of the ruminal fluid sample and enriched some poorly characterized ruminal bacteria allowing exploration of their genomic potential and their functional role in the rumen ecosystem.

Evolutionary Wheat Populations in High-Quality Breadmaking as a Tool to Preserve Agri-Food Biodiversity.

Plant biodiversity preservation is one of the most important priorities of today’s agriculture. Wheat (Triticum spp. L.) is widely cultivated worldwide, mostly under a conventional and monovarietal farming method, leading to progressive biodiversity erosion. On the contrary, the evolutionary population (EP) cultivation technique is characterized by mixing and sowing together as many wheat genotypes as possible to allow the crop to genetically adapt over the years in relation to specific pedoclimatic conditions. The objective of this study was to assess the nutritional, chemical and sensory qualities of three different breads obtained using different organic EP flours, produced following a traditional sourdough process and compared to a commercial wheat cultivar bread. Technological parameters, B-complex vitamins, microelements, dietary fibre and phenolic acids were determined in raw materials and final products. Flours obtained by EPs showed similar characteristics to the commercial wheat cultivar flour. However, significant differences on grain technological quality were found. The breads were comparable with respect to chemical and nutritional qualities. Overall, the sensory panellists rated the tasted breads positively assigning the highest score to those produced with EPs flours (6.75–7.02) as compared to commercial wheat cultivar-produced bread (cv. Bologna, 6.36).

Quantitative analysis of vitamins B2, B3, B5 and B6 by HPLC-UV in selected non-leafy vegetables

ABSTRACT Renewed interest in rapid, accurate, quantification of vitamins in foodstuffs is due to more stringent requirements by food regulatory agencies. The study was conducted to determine vitamin B2, B3, B5 and B6 content of the non-leafy vegetables bitter gourd (Momordica charantia L.), pointed gourd (Trichosanthes dioica Roxb.), green chili (Capsicum annum L.) and pumpkin (Cucurbita maxima Duch.) by HPLC-UV. These non-leafy vegetables have different levels of vitamin-B complexes that can be used for value addition of vitamin B-groups and can be used for calculating the habitual dietary intake.

Biological Properties of Vitamins of the B-Complex, Part 1: Vitamins B1, B2, B3, and B5.

This review summarizes the current knowledge on essential vitamins B1, B2, B3, and B5. These B-complex vitamins must be taken from diet, with the exception of vitamin B3, that can also be synthetized from amino acid tryptophan. All of these vitamins are water soluble, which determines their main properties, namely: they are partly lost when food is washed or boiled since they migrate to the water; the requirement of membrane transporters for their permeation into the cells; and their safety since any excess is rapidly eliminated via the kidney. The therapeutic use of B-complex vitamins is mostly limited to hypovitaminoses or similar conditions, but, as they are generally very safe, they have also been examined in other pathological conditions. Nicotinic acid, a form of vitamin B3, is the only exception because it is a known hypolipidemic agent in gram doses. The article also sums up: (i) the current methods for detection of the vitamins of the B-complex in biological fluids; (ii) the food and other sources of these vitamins including the effect of common processing and storage methods on their content; and (iii) their physiological function.

Unraveling of Chlorella-associated bacterial load, diversity, and their imputed functions at high- and low-yield conditions through metagenome sequencing.

Chlorella-associated bacteria can have a significant influence on facilitating higher Chlorella biomass yield due to their symbiotic relationship. In this study, non-axenic Chlorella was cultivated in an airlift photobioreactor at high and low-yield conditions. The associated bacterial diversity was analyzed using 16S rRNA metagenome sequencing. At high-yield conditions, the bacterial load was observed in the range of 108 -1010 CFU · mL-1 , whereas at low-yield conditions, bacteria were more dominant and observed in the range of 1014 -1015 CFU · mL-1 . The majority of the bacterial species associated with Chlorella at high-yield conditions belongs to Proteobacteria and Bacteroidetes. Further, Bacteroidetes levels were decreased at low-yield conditions and were highly diversified with Planctomycetes, Firmicutes, and 18 others. Predicted functional genes indicated that Chlorella-associated bacteria have the enzymes involved in the metabolism and biosynthesis of B-complex vitamins (i.e., vitamin B12 , thiamin, biotin, pyridoxine, and riboflavin). A critical evaluation revealed that vitamin biosynthesis genes were more abundant at low-yield conditions; however, vitamin B12 transport genes (B12 transport ATP-binding protein, B12 substrate-binding transportation, and B12 permease protein) were less abundant, indicating even though vitamins production occurs, but their availability to Chlorella was limited due to the lack of vitamin transport genes. Further, at high yield, Chlorella-associated bacteria enabled higher growth by supplementing the vitamins. In contrast, at low-yield condition-an increased bacterial load, diversity, and limited vitamin transport functional genes affected the Chlorella yield. It can be inferred that Chlorella yield was significantly affected by three factors: associated bacterial load, diversity, and transport functional genes of vitamins.

Population perception of use of folic acid among females during childbearing period in the Eastern Region, Saudi Arabia

Background: Folic acid (FA) is a B-complex vitamin that has a vital role for pregnant women and the fetus in the development of the skull and central nervous system. This study aims to assess the level of knowledge about folic supplements among females during the childbearing period in the eastern region of Saudi Arabia. Methods: A qualitative cross-sectional based on 385 female participants aged 18 to 45 was conducted between 1 January 2021 and 29 August 2021. An online electronic survey was employed using social media in the eastern region of Saudi Arabia for this study. Result: This study found that 10.9% of participants had good knowledge of FA, and 89.1% had poor knowledge. Most of the participants (96.1%) heard about FA. Among them, 36.1% knew that FA is vitamin B. Only 75 (19.5%) females reported getting the proper daily amount of FA (400 mg/day), and 216 (56.1%) knew that FA should be given before and during the first trimester of pregnancy. Regarding the effects of FA deficiency, 51.2% of females reported FA deficiency anemia. Only 22.1% of females said they had heard of neural tube defects. Conclusion: This study concluded that the female population&amp;apos;s knowledge regarding FA is poor. Authors suggest using social media and awareness campaigns to improve the understanding and knowledge of FA among Saudi female people.

Pre-conceptional Maternal Vitamin B12 Supplementation Improves Offspring Neurodevelopment at 2 Years of Age: PRIYA Trial.

Background: The first thousand days window does not include the pre-conceptional period. Maternal pre-conceptional health has a profound influence on early embryonic development (implantation, gastrulation, placentation etc). Nutrition provided by B-complex vitamins is important for fetal growth, especially neural development. We report effects of a maternal pre-conceptional vitamin B12 and multi micronutrient (MMN) supplementation on offspring neurodevelopmental performance.Methods: In the Pune Rural Intervention in Young Adolescents trial (PRIYA), adolescents (N = 557, 266 females) were provided with vitamin B12 (2 μg/day) with or without multiple micronutrients, or a placebo, from preconception until delivery. All groups received mandatory iron and folic acid. We used the Bayley's Scale of Infant Development (BSID-III) at 24–42 months of age to investigate effects on offspring neurodevelopment.Results: Participants had similar baseline B12 levels. The levels improved in the B12 supplemented groups during pre-conception and pregnancy (28 weeks gestation), and were reflected in higher cord blood holotranscobalamin (holo-TC) levels compared to the placebo group. Neurodevelopmental outcomes in the B12 alone group (n = 21) were better than the placebo (n = 27) in cognition (p = 0.044) and language (p = 0.020) domains (adjusted for maternal baseline B12 levels). There was no difference in neurodevelopmental outcomes between the B12 + MMN (n = 26) and placebo group. Cord blood Brain Derived Neurotrophic Factor (BDNF) levels were highest in the B12 alone group, though not significant.Conclusion: Pre-conceptional vitamin B12 supplementation improved maternal B12 status and offspring neurodevelopment at 2 years of age. The usefulness of cord BDNF as a marker of brain development needs further investigation. Our results highlight the importance of intervening during pre-conception.

Review on Pharmacognosy, phytochemistry and pharmacological activity of Taraxacum officinale (Dandelion plant)

Taraxacum officinale (dandelion), a member of the Asteraceae family, commonly found in the temperate zone of the Northern hemisphere, is the perennial herb, forming a rosette leaf, golden yellow flowers that bloom year round. Dandelion leaves, roots and flowers is used in many traditional and modern herbal medicinal systems. On phytochemical investigation, Dandelion herb contains one or more vital phytochemical constituents. Similarly Among the most important compounds in dandelion Contains sesquiterpene lactones which is a bitter element, principally contains taraxacin and taraxacerin, which is principally important in mechanism of liver functions related with hepatoprotective action. Besides that result of the phytochemical investigation revealed the presence of sterols, phenolic acids, flavonoids, that have been shown to be associated with numerous pharmacological effects such as Antioxidants, anti immflammatory, antidiabetics, antitumor activity. Along with that the Dandelion leaves are a high efficient source of vitamins and minerals, including beta carotene, non-provitamin A carotenoids, xanthophylls, chlorophyll, vitamins C and D, many of the B-complex vitamins, choline, iron, silicon, magnesium, sodium, potassium, zinc, manganese, copper, and phosphorous which is also useful as a several nutritive action. This review will point out areas for future research of phytochemicals containing Taraxacum officinale where they shows more effective against various ailments and shows recent effective pharmacological activity

Use of B-complex vitamins and olfactory training for treating COVID-19-related anosmia.

A 42‐year‐old male patient was diagnosed with coronavirus disease 2019. His symptoms improved 2 weeks after lopinavir therapy (800 mg/day). However, he was subsequently diagnosed with complete anosmia. Magnetic resonance brain imaging showed no abnormalities. We prescribed B‐complex vitamins and olfactory training. Forty days later, he recovered.

Restrictions and supplementations effects of vitamins B6, B9 and B12 on growth, vasculogenesis and senescence of BG01V human embryonic stem cell derived embryoid bodies

Of biologically active B-complex vitamins, vitamins B6, B9 and B12 has been reported to influence maintenance of red blood cells, stem cells, nervous and immune systems, tissue regeneration and wound healing. However, the collective effects of restrictions/supplementations of vitamins B6, B9 and B12 on growth, vasculogenesis and senescence of BG01V human embryonic stem cell (hESC) derived human embryoid bodies (hEBs) is not understood. This study was proposed to understand the collective effects of restrictions/supplementations of vitamins B6, B9 and B12 on growth, vasculogenesis and senescence of hEBs. The collective effect of four different media conditions like deficient (0%), marginal (30%), adequate (70%) and supraphysiological levels of vitamins B6, B9 and B12 restrictions/supplementations on growth and viability of hEB derived vasculogenesis were studied by photometric analysis. Morphology and senescence of hEB derivatives were examined and compared using inverted microscopy and scanning electron microscopy. Out of four different media conditions like 0%, 30%, 70% and supraphysiological levels of vitamins B6, B9 and B12, media supplied with adequate level of vitamins B6, B9 and B12 to hESCs grown on 100% restriction were able to resume all activities at normal. The BG01V hESC-hEBs grown without vitamins B6, B9 and B12 showed cell disintegration, cell floating, SA-β-gal expression, cell lose, decrease size, number and metabolic activity. The hEB derived vascular cell like structures grown on similar conditions showed compromised self-renewal capacity, vulnerable growth and early senescence. The initial damages due to restrictions may be reversed by adequate (70%) supply of vitamins B6, B9 and B12; however reversal of damage may not be 100%.

Goat meat products

In general, goat meat is not inferior to other meat types regarding nutritional and biological value–it has a high protein content (up to 29%), and it is a good source of minerals, vitamin B-complex, and essential amino acids. However, the meat of older and culled goats is less juicy, less tender, has a characteristically different odour and taste compared to kids’ goat meat (and meat of other animals), and thus tends to be less desirable. Different meat products could be produced using goat meat (including culled goat meat): dry-fermented sausages (e.g. sucuk), dry-cured meats (Violino di capra–goat prosciutto), frankfurters, mortadella, etc. without adverse effects on products' technological properties. The negative impact of goat meat on the properties of meat products is mainly associated with the use of goat fatty tissue. However, this could be overcome by using fatty tissue of other animals (e.g. pork back fat or beef fatty tissue).

Microbial analysis on biotin nutrition bar with different packaging material

Biotin a vitamin of the B-complex vitamin family well known to play a major role to improve skin, hair growth, and nails. A nutrition bar with rich biotin content is prepared. Based on this, a study was conducted using various food materials wherein three samples were prepared with different compositions and proportion. Sensory evaluation was done by the sensory panelist and scores and one sample is selected. The main sample was prepared with the selected sample combination and proportion. Then the main sample was packed in three different packaging materials and stored in two different temperature conditions. After the 15th and 30th days of packing, microbial analysis was done. By calculating the microbial analysis, sample packed in aluminum foil has a less bacterial forming colony in both room and refrigerant temperature, when compared to sample packed in low-density polyethylene and plastic bottle. The sample was found edible even after 30 days of packing as jaggery was used as a preservative and cardamom as flavoring agent having anti-fungal and anti-bacterial properties.

Promotor Anchored - RAPD Analysis of Foxtail Millet (Setaria italica L.) Accessions Selected For High Iron and Zinc Content

Foxtail millet (Setaria italica L.) is nutri-cereal crop having it is rich in β-carotene, vitamin B-complex and micronutrients like minerals. In the present research, we have studied biochemical properties and molecular profiling to identify the core set of foxtail millet (Setaria italic L.) accessions for high Iron (Fe) and Zinc (Zn) content. Total seventy-nine accessions and selected mutants variety PS4 of foxtail millet were used. The biochemical investigation revealed that accessions M2-106, IC120407, M3-61/HB-13, and IC120255 consist of high iron and zinc content. The genetic variability among the genotypes was revealed by 28 Promoter Anchored Amplified Polymorphic (PAAP-RAPD) primers of which OPE9+GC1, OPE9+CA1, UBC001+CA1, UBC001+TA1, UBC693+G1 showed 100% polymorphism, whereas UBC693+GC1 and OPE7+G1 showed 88% and 80% polymorphism, respectively, with an average of 45.95 % polymorphism. Total alleles per locus were 3.31, whereas, the average number of monomorphic and polymorphic alleles were 1.72 and 1.56, respectively. The extent of polymorphic information content (PIC) of PAAP-RAPD loci ranged from 0.5 to 0.87 with an average value of 0.41. For PAAP-RAPD, the maximum PIC value was observed in marker UBC693+GC1 (0.87 %) and the minimum were OPE5+CA1 and OPE9+CA1 (0.5 %). Molecular characterizations result showing highest similarity (0.932) between accessions Shrilakshmi and Prasad, whereas, the lowest similarity coefficient was observed between IC120255 and M3-75/AM-1 (0.697) with PS4.UPGMA dendrogram grouped the foxtail millet accessions in five clusters which marked high diversity in M3-61/HB-13 and M3-75/AM-1. It implies that PAAP-RAPD markers are significantly screened in the foxtail millet accessions and have enumerated high genetic diversity.