In this article, the eye-in-hand visual trajectory tracking control problem of wheeled mobile robots (WMRs) is considered. Different from the conventional vision-based approaches, the monocular camera is not required to be mounted at the center of WMR, and thus the derived visual model is subject to not only the nonholonomic constraint but also the unknown camera extrinsic parameters. To ensure the WMR can track a desired trajectory effectively, a combined observation/control strategy is proposed. First, a concurrent learning observer is designed to identify the camera extrinsic parameters with measurable visual signals. Then, with the aid of the estimated parameters, a nonlinear controller is presented to achieve the tracking task. The closed-loop system stability is analyzed with Lyapunov methods, showing that both the estimation and tracking errors are asymptotically convergent to zero. Simulation and experimental results are provided to validate the developed approach.