Visual Stimuli Research Articles

Validation of sound source localization quality in binaural audio synthesis with head-tracking

Binaural synthesis with head tracking has become a highly important tool in creating an auditory experience for users that is as similar as possible to their perception of sound in the real world. This is crucial in many different applications that rely on accurate representation of real or virtual sound environments, ranging from scientific research to gaming applications. The quality of the binaural synthesis process and the accuracy and naturalness of the produced result depend on numerous factors, ranging from the selection of suitable IMU sensor technology used for head tracking to the choice of methods for implementing binaural synthesis using generic or individual sets of Head-Related Transfer Functions (HRTF). This is especially significant in Virtual Reality (VR) systems with three or six degrees of freedom that fully immerse the users in the virtual world through visual and auditory stimuli. This paper presents the authors' experiences in addressing a variety of challenges that arose during the design of such spatial audio systems and the testing of sound source localization quality. Strong emphasis is put on the testing of sound source localization quality, where the position of the source dynamically changes in relation to the listener.

Functional specialization and distributed processing across marmoset lateral prefrontal subregions.

A prominent aspect of primate lateral prefrontal cortex organization is its division into several cytoarchitecturally distinct subregions. Neurophysiological investigations in macaques have provided evidence for the functional specialization of these subregions, but an understanding of the relative representational topography of sensory, social, and cognitive processes within them remains elusive. One explanatory factor is that evidence for functional specialization has been compiled largely from a patchwork of findings across studies, in many animals, and with considerable variation in stimulus sets and tasks. Here, we addressed this by leveraging the common marmoset (Callithrix jacchus) to carry out large-scale neurophysiological mapping of the lateral prefrontal cortex using high-density microelectrode arrays, and a diverse suite of test stimuli including faces, marmoset calls, and spatial working memory task. Task-modulated units and units responsive to visual and auditory stimuli were distributed throughout the lateral prefrontal cortex, while those with saccade-related activity or face-selective responses were restricted to 8aV, 8aD, 10, 46V, and 47. Neurons with contralateral visual receptive fields were limited to areas 8aV and 8aD. These data reveal a mixed pattern of functional specialization in the lateral prefrontal cortex, in which responses to some stimuli and tasks are distributed broadly across lateral prefrontal cortex subregions, while others are more limited in their representation.

Unveiling Visual Physiology and Steady-State Evoked Potentials using Low-Cost and Transferable Electroencephalography for Evaluating Neuronal Activation

Purpose: The ability to see and process images depends on the function of the eyes and the processing of visual information by neurons in the cerebral cortex, something that could be measured through electroencephalography (EEG). Although the EEG is used to evaluate visual pathways in children and demyelination diseases, the limited utilization of brain recording techniques in other applications like therapy is primarily due to budget constraints. The goal of this paper is to demonstrate results from studying brain aspects of vision, utilizing measurements based on oscillatory activity analysis, low-cost, portable equipment, and a processing pipeline relying on Python’s open-source libraries. These studies involve healthy subjects who wear glasses to assess changes in visual perception. Methods: First, electroencephalographic signals were recorded while the subjects observed a visually standardized stimulus. The signals were processed and filtered to reduce artifacts, and the power spectral density (PSD) was calculated to observe the presence of steady-state visual potentials (VEP) to confirm the capture of neuronal activation to the visual stimulus. Results: It was possible to establish a difference between subjects wearing and not wearing their glasses, allowing validation that the information acquired with the transferable equipment is adequate for the analysis of neuronal activity related to visual processing, opening the possibility to be used in future studies in therapy. Conclusion: This study contributes to the development of cost-effective and portable EEG solutions for visual system analysis. It demonstrates the potential for applying transferable EEG devices in clinical settings and highlights the importance of tailored visual stimuli for reliable neural activation.

Differences in Mu rhythm when seeing grasping/motor actions in a real context versus on screens

Mu rhythm (∼8–12 Hz) in the somatosensory cortex has traditionally been linked with doing and seeing motor activities. Here, we aimed to learn how the medium (physical or screened) in which motor actions are seen could impact on that specific brain rhythm. To do so, we presented to 40 participants the very same narrative content both in a one-shot movie with no cuts and in a real theatrical performance. We recorded subjects’ brain activities with electroencephalographic (EEG) procedures, and analyzed Mu rhythm present in left (C3) and right (C4) somatosensory areas in relation to the 24 motor activities included in each visual stimulus (screen vs. reality) (24 motor and grasping actions x 40 participants x 2 conditions = 1920 trials). We found lower Mu spectral power in the somatosensory area after the onset of the motor actions in real performance than on-screened content, more pronounced in the left hemisphere. In our results, the sensorimotor Mu-ERD (event-related desynchronization) was stronger during the real-world observation compared to screen observation. This could be relevant in research areas where the somatosensory cortex is important, such as online learning, virtual reality, or brain-computer interfaces.

Modulation of face processing by top-down attention: Insights from early ERP waveforms

The face fusiform area (FFA) plays a pivotal role in face recognition, yet the precise timeline of its activity remains debated. Using EEG, we conducted three experiments to investigate how expectancy-consistent versus expectancy-inconsistent visual stimuli influence processing dynamics. Participants viewed images of faces, houses, and tools (Experiment 1), celebrity faces (Experiment 2), or animal faces (Experiment 3), preceded by a priming question. Notably, both conditions presented identical visual stimulation, ensuring that observed differences stemmed from cognitive processing rather than sensory input. Our results from Experiments 2 and 3 reveal that while the initial 150 ms period, crucial for unconscious face detection, remained unaffected, subsequent processing exhibited a delay of several milliseconds for expectancy-inconsistent stimuli, indicating additional processing time required for unexpected recognition. Importantly, no significant differences were observed in Experiment 1, where less demanding tasks or generic mental imagery were used, suggesting that the priming effect was not as pronounced in this context. These findings underscore the critical role of the period immediately following the first 150 ms in face identification and individuation, highlighting the influence of top-down attention on face recognition dynamics. This study provides novel insights into the temporal dynamics of face processing and the neural mechanisms underlying top-down attentional modulation.

Predicting visual function by interpreting a neuronal wiring diagram

As connectomics advances, it will become commonplace to know far more about the structure of a nervous system than about its function. The starting point for many investigations will become neuronal wiring diagrams, which will be interpreted to make theoretical predictions about function. Here I demonstrate this emerging approach with the Drosophila optic lobe, analysing its structure to predict that three Dm3 (refs. 1–4) and three TmY (refs. 2,4) cell types are part of a circuit that serves the function of form vision. Receptive fields are predicted from connectivity, and suggest that the cell types encode the local orientation of a visual stimulus. Extraclassical5,6 receptive fields are also predicted, with implications for robust orientation tuning7, position invariance8,9 and completion of noisy or illusory contours10,11. The TmY types synapse onto neurons that project from the optic lobe to the central brain12,13, which are conjectured to compute conjunctions and disjunctions of oriented features. My predictions can be tested through neurophysiology, which would constrain the parameters and biophysical mechanisms in neural network models of fly vision14.

Sensitization of Visually Induced Motion Sickness by Prior Provocative Physical Motion

INTRODUCTION: Habituation to motion has therapeutic applications for motion sickness desensitization and rehabilitation of patients with vestibular disease. Less attention has been devoted to the opposite process: sensitization. METHODS: Subjects (N = 50) were randomly allocated to four sequences: Baseline visual stimulus; then 15 min of time gap; cross-coupled motion (C-C) or a Control condition; then a time gap of 15 min or 2 h; then a retest visual stimulus. Motion exposures were for 10 min or until moderate nausea, whichever was sooner. The visual stimulus was a scene rotating in yaw at 0.2 Hz with superimposed "wobble". C-C was whole-body rotation on a turntable with eight 45° head tilts during each 30-s period. Control was head tilt without rotation. Rotational velocity was incremented in staircase steps of 3° · s−1 every 30 s. RESULTS: Groups were equivalent for Total Motion Sickness Symptom scores elicited by the first visual stimulus (combined: mean ± SD 10.8 ± 8.4). C-C produced greater Total Symptoms (20.3 ± 6.8) than Control (3.1 ± 3.7). Subjects recovered subjectively from C-C before retest of visual stimulus. For the retest visual stimulus, Total Symptoms were higher following C-C (15.1 ± 9.0) than following Control (8.3 ± 7.1) for both the 15 min and 2 h retests. Sickness ratings (SR) mirrored these effects of C-C. DISCUSSION: C-C motion sensitized subsequent responses to visual stimulation up to 2 h later. Sensitization of visual stimulation crossed modalities and appeared subconscious since it occurred despite subjective recovery from C-C. For some individuals, a previously relatively innocuous visual stimulus became nauseogenic on retest. The results have implications for the use of visual technologies within hours of exposure to provocative motion. Golding JF, Alund D, Gresty MA, Flynn MB. Sensitization of visually induced motion sickness by prior provocative physical motion. Aerosp Med Hum Perform. 2024; 95(10):741–748.

Spatial context non-uniformly modulates inter-laminar information flow in the primary visual cortex.

Our visual experience is a result of the concerted activity of neuronal ensembles in the sensory hierarchy. Yet, how the spatial organization of objects influences this activity remains poorly understood. We investigate how inter-laminar information flow within the primary visual cortex (V1) is affected by visual stimuli in isolation or with flankers at spatial configurations that are known to cause non-uniform degradation of perception. By employing dimensionality reduction approaches to simultaneous, layer-specific population recordings, we establish that information propagation between cortical layers occurs along a structurally stable communication subspace. The spatial configuration of contextual stimuli differentially modulates inter-laminar communication efficacy, the balance of feedforward and effective feedback signaling, and contextual signaling in the superficial layers. Remarkably, these modulations mirror the spatially non-uniform aspects of perceptual degradation. Our results suggest a model of retinotopically non-uniform cortical connectivity in the output layers of V1 that influences information flow in the sensory hierarchy.

Rhythmic Temporal Cues Coordinate Cross-frequency Phase-amplitude Coupling during Memory Encoding.

Accumulating evidence suggests that rhythmic temporal cues in the environment influence the encoding of information into long-term memory. Here, we test the hypothesis that these mnemonic effects of rhythm reflect the coupling of high-frequency (gamma) oscillations to entrained lower-frequency oscillations synchronized to the beat of the rhythm. In Study 1, we first test this hypothesis in the context of global effects of rhythm on memory, when memory is superior for visual stimuli presented in rhythmic compared with arrhythmic patterns at encoding [Jones, A., & Ward, E. V. Rhythmic temporal structure at encoding enhances recognition memory, Journal of Cognitive Neuroscience, 31, 1549-1562, 2019]. We found that rhythmic presentation of visual stimuli during encoding was associated with greater phase-amplitude coupling (PAC) between entrained low-frequency (delta) oscillations and higher-frequency (gamma) oscillations. In Study 2, we next investigated cross-frequency PAC in the context of local effects of rhythm on memory encoding, when memory is superior for visual stimuli presented in-synchrony compared with out-of-synchrony with a background auditory beat (Hickey et al., 2020). We found that the mnemonic effect of rhythm in this context was again associated with increased cross-frequency PAC between entrained low-frequency (delta) oscillations and higher-frequency (gamma) oscillations. Furthermore, the magnitude of gamma power modulations positively scaled with the subsequent memory benefit for in- versus out-of-synchrony stimuli. Together, these results suggest that the influence of rhythm on memory encoding may reflect the temporal coordination of higher-frequency gamma activity by entrained low-frequency oscillations.

Evaluating the Effects of Different Cognitive Tasks on Autonomic Nervous System Responses: Implementation of a High-Precision, Low-Cost Complementary Method.

We developed a low-cost, user-friendly complementary research tool to evaluate autonomic nervous system (ANS) activity at varying levels of cognitive workload. This was achieved using visual stimuli as cognitive tasks, administered through a specially designed computer-based test battery. To assess sympathetic stress responses, skin conductance response (SCR) was measured, and electrocardiograms (ECG) were recorded to evaluate heart rate variability (HRV), an indicator of cardiac vagal tone. Twenty-five healthy adults participated in the study. SCR and ECG recordings were made during both tonic and phasic phases using a computer-based system designed for visual stimuli. Participants performed a button-pressing task upon seeing the target stimulus, and the relationship between reaction time (RT) and cognitive load was evaluated. Analysis of the data showed higher skin conductance levels (SCLs) during tasks compared to baseline, indicating successful elicitation of sympathetic responses. RTs differed significantly between simple and cognitive tasks, increasing with mental load. Additionally, significant changes in vagally mediated HRV parameters during tasks compared to baseline highlighted the impact of cognitive load on the parasympathetic branch of the ANS, thereby influencing the brain-heart connection. Our findings indicate that the developed research tool can successfully induce cognitive load, significantly affecting SCL, RTs, and HRV. This validates the tool's effectiveness in evaluating ANS responses to cognitive tasks.

The face pareidolia illusion drives a happy face advantage that is dependent on perceived gender.

The happy face advantage, the faster recognition of happy than of negative, angry or fearful, emotional expressions, has been reliably found and is modulated by social category cues, such as perceived gender, that is, is larger on female than on male faces. In this study, we tested whether this pattern of results is unique to human faces by investigating whether ambient examples of face pareidolia can also evoke a happy face advantage that is dependent on perceived gender. "Face pareidolia" describes the illusion of facial structure on inanimate objects, such as a tree trunk or a piece of burnt toast. While it has been shown that these illusory faces have expressions that can be recognized by participants, it is unknown whether they drive the same behavioral biases as real facial expressions. Thus, we measured the speed and accuracy with which the expressions of illusory faces that are perceived as female or male are recognized as happy or angry. We found a robust happy face advantage for illusory faces that were rated as more feminine in appearance. Concomitantly, we also found a robust angry face advantage for illusory faces that were rated as more masculine in appearance. Taken together, these findings demonstrate that illusory faces confer the same behavioral advantages as human faces. They also suggest that both perceived emotion and perceived gender are powerful socioevaluative dimensions that are extracted from visual stimuli that merely resemble human faces. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Investigating causal effects of pupil size on visual discrimination and visually evoked potentials in an optotype discrimination task.

Pupil size primarily changes to regulate the amount of light entering the retina, optimizing the balance between visual acuity and sensitivity for effective visual processing. However, research directly examining the relationship between pupil size and visual processing has been limited. While a few studies have recorded pupil size and EEG signals to investigate the role of pupil size in visual processing, these studies have predominantly focused on the domain of visual sensitivity. Causal effects of pupil size on visual acuity, therefore, remain poorly understood. By manipulating peripheral background luminance levels and target stimulus contrast while simultaneously recording pupillometry and EEG signals, we examined how absolute pupil size affects visual discrimination and visually evoked potentials (VEP) in a task using optotype mimicking the Snellen eye chart, the most common assessment of visual acuity. Our findings indicate that both higher background luminance levels and higher target contrast were associated with improved target discrimination and faster correct reaction times. Moreover, while higher contrast visual stimuli evoked larger VEPs, the effects of pupil size on VEPs were not significant. Additionally, we did not observe inter-individual correlations between absolute pupil size and discrimination performance or VEP amplitude. Together, our results demonstrate that absolute pupil size, regulated by global luminance level, played a functional role in enhancing visual discrimination performance in an optotype discrimination task. The differential VEP effects of pupil size compared to those of stimulus contrast further suggested distinct neural mechanisms involved in facilitating visual acuity under small pupils.

Local stimulation of pyramidal neurons in deep cortical layers of anesthetized rats enhances cortical visual information processing

In the primary visual cortex area V1 activation of inhibitory interneurons, which provide negative feedback for excitatory pyramidal neurons, can improve visual response reliability and orientation selectivity. Moreover, optogenetic activation of one class of interneurons, parvalbumin (PV) positive cells, reduces the receptive field (RF) width. These data suggest that in V1 the negative feedback improves visual information processing. However, according to information theory, noise can limit information content in a signal, and to the best of our knowledge, in V1 signal-to-noise ratio (SNR) has never been estimated following either pyramidal or inhibitory neuron activation. Therefore, we optogenetically activated pyramidal or PV neurons in the deep layers of cortical area V1 and measured the SNR and RF area in nearby pyramidal neurons. Activation of pyramidal or PV neurons increased the SNR by 267% and 318%, respectively, and reduced the RF area to 60.1% and 77.5%, respectively, of that of the control. A simple integrate-and-fire neuron model demonstrated that an improved SNR and a reduced RF area can increase the amount of information encoded by neurons. We conclude that in V1 activation of pyramidal neurons improves visual information processing since the location of the visual stimulus can be pinpointed more accurately (via a reduced RF area), and more information is encoded by neurons (due to increased SNR).

Investigating the human capabilities for intuitive evaluation of component manufacturability

This article explores the intersection of Design for Manufacturing (DFM) principles and human cognition in component manufacturability. In the context of contemporary manufacturing challenges, where design and manufacturing departments often operate separately, this study aims to bridge the gap by investigating human capabilities in intuitively assessing manufacturability during the design phase. Research employs experimental trials with visual stimuli representing various part designs, categorizing them based on adherence to DFM rules. Participants, without prior formal education in DFM, are tasked with choosing the more manufacturable component within a pair. Results indicate that a majority of participants demonstrate an innate ability to make correct manufacturability assessments. Additionally, the study evaluates the impact of stimulus exposure time on participant performance. These findings provide valuable insights into the intuitive aspects of human manufacturability assessment and lay the groundwork for further research, including larger participant groups and deeper exploration of decision-making processes and knowledge types involved in DFM practices. The main contribution of presented study involves showcasing human capabilities for component manufacturability assessment with the use of intuitive thinking and quick decision-making in place of following strict rulesets and design principles.

Visual Perceptual Learning of Form-Motion Integration: Exploring the Involved Mechanisms with Transfer Effects and the Equivalent Noise Approach.

Background: Visual perceptual learning plays a crucial role in shaping our understanding of how the human brain integrates visual cues to construct coherent perceptual experiences. The visual system is continually challenged to integrate a multitude of visual cues, including form and motion, to create a unified representation of the surrounding visual scene. This process involves both the processing of local signals and their integration into a coherent global percept. Over the past several decades, researchers have explored the mechanisms underlying this integration, focusing on concepts such as internal noise and sampling efficiency, which pertain to local and global processing, respectively. Objectives and Methods: In this study, we investigated the influence of visual perceptual learning on non-directional motion processing using dynamic Glass patterns (GPs) and modified Random-Dot Kinematograms (mRDKs). We also explored the mechanisms of learning transfer to different stimuli and tasks. Specifically, we aimed to assess whether visual perceptual learning based on illusory directional motion, triggered by form and motion cues (dynamic GPs), transfers to stimuli that elicit comparable illusory motion, such as mRDKs. Additionally, we examined whether training on form and motion coherence thresholds improves internal noise filtering and sampling efficiency. Results: Our results revealed significant learning effects on the trained task, enhancing the perception of dynamic GPs. Furthermore, there was a substantial learning transfer to the non-trained stimulus (mRDKs) and partial transfer to a different task. The data also showed differences in coherence thresholds between dynamic GPs and mRDKs, with GPs showing lower coherence thresholds than mRDKs. Finally, an interaction between visual stimulus type and session for sampling efficiency revealed that the effect of training session on participants' performance varied depending on the type of visual stimulus, with dynamic GPs being influenced differently than mRDKs. Conclusion: These findings highlight the complexity of perceptual learning and suggest that the transfer of learning effects may be influenced by the specific characteristics of both the training stimuli and tasks, providing valuable insights for future research in visual processing.

The P300 and hierarchical dimensions of psychopathology.

The Hierarchical Taxonomy of Psychopathology (HiTOP) framework offers the potential to better understand how neurobiological mechanisms relate to psychopathology. The P300 is an event-related potential component that indexes attention, stimulus evaluation, and categorization. A blunted P300 has been associated with psychiatric disorders across externalizing, internalizing, and thought disorder domains. However, there has been little research investigating whether the P300 is related to higher-order dimensions of psychopathology. In a sample of 225 adults aged 18-35 years (Mage = 23.09, SD = 3.83, 82.2% female) who were oversampled for psychopathology, the present study examined the associations between the P300 and both psychopathology spectra and a general factor. Participants completed multiple experimental tasks, while electroencephalography was recorded to measure the P300 elicited by auditory, tactile, and visual stimuli. Participants also completed the self-report Comprehensive Assessment of Traits Relevant to Personality Disorder to assess pathological personality dimensions. We used structural equation modeling to examine the relationship between a latent P300 factor and both psychopathology spectra (negative emotionality, detachment, psychoticism, disinhibition, and antagonism) and a general factor. The results indicated that the P300 was inversely related to the general factor. Further analyses revealed no relationships between the P300 and any individual spectrum when accounting for the general factor. Overall, the present study indicates that the P300 is associated with the general factor of psychopathology, which might explain its association with multiple categorical disorders. The study also demonstrates the potential importance of neuroscience-informed dimensional systems to understand clinical phenomena. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

"Hot" affect-related aspects in emotional information processing: The role of facial muscle responses in the direct and indirect processing of emotion categories.

The present study investigated the involvement of facial muscle responses in the indirect and direct processing of emotional facial expressions. Five discrete emotion categories were used, and we assessed both facial muscle and behavioral responses on a trial-by-trial basis. Experiment 1 tested facial muscle activation of clearly visible stimuli in an emotion categorization task. We observed emotion-specific facial muscle responses and corresponding behavioral categorization effects, providing evidence for the specificity of facial muscle activation. By contrast, under masked indirect presentation conditions in which emotional facial expressions were presented as primes in an emotion misattribution procedure, a specific pattern of emotion-congruent and cross-category behavioral misattributions was observed (in line with Rohr et al., 2015, 2018). Multilevel analyses in Study 2 suggest that an emotional reaction feeds into the behavioral decision, as indicated by differential activation of the frontalis lateralis in response to angry faces. Thus, the present study provides evidence that facial muscle responses contribute to behavioral decisions under masked indirect processing conditions. The different pattern of effects in both studies suggests that facial muscle responses index different processes, depending on the processing conditions: sensorimotor simulation in direct processing conditions and emotional reactions in masked indirect processing conditions. We discuss the implications for models that aim to account for facial muscle activity in response to emotional facial expressions. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Pedestrian Behavior in Static and Dynamic Virtual Road Crossing Experiments

Virtual studies involving pedestrians have gained relevance due to the advantage of not exposing them to actual risk, and simulation setups have benefitted from rapid technical advancements, becoming increasingly complex and immersive. However, it remains unclear whether complex setups affecting participants’ freedom of movement impact their decision-making. This research evaluated the effects of a more realistic approach to studying pedestrian crossing behavior by comparing a perception-action task requiring participants to walk effectively along a semi-virtual crosswalk with a similar experiment using static crossing conditions. Using a CAVE system, two real-world streets were modeled in two different virtual scenarios, varying vehicle speed patterns and distance from the crosswalk. Visual stimuli were presented to two groups of 30 participants, with auditory stimuli adapted accordingly. The impact of various factors on participants’ crossing decisions was evaluated by examining the percentage of crossings, crossing start time, and time-to-passage. Overall, the experimental approach did not significantly affect participants’ crossing decisions.

Neural Signature and Decoding of Unmanned Aerial Vehicle Operators in Emergency Scenarios Using Electroencephalography.

Brain-computer interface (BCI) offers a novel means of communication and control for individuals with disabilities and can also enhance the interactions between humans and machines for the broader population. This paper explores the brain neural signatures of unmanned aerial vehicle (UAV) operators in emergencies and develops an operator's electroencephalography (EEG) signals-based detection method for UAV emergencies. We found regularity characteristics similar to classic event-related potential (ERP) components like visual mismatch negativity (vMMN) and contingent negative variation (CNV). Source analysis revealed a sequential activation of the occipital, temporal, and frontal lobes following the onset of emergencies, corresponding to the processing of attention, emotion, and motor intention triggered by visual stimuli. Furthermore, an online detection system was implemented and tested. Experimental results showed that the system achieved an average accuracy of over 88% in detecting emergencies with a detection latency of 431.95 ms from the emergency onset. This work lays a foundation for understanding the brain activities of operators in emergencies and developing an EEG-based detection method for emergencies to assist UAV operations.

ANALISIS KONTEN HOTS DALAM BUKU SIMPAY BASA SUNDA KELAS VIII

The goal of 21st century learning is that students are expected to have higher order thinking skills. But in fact, students' high-level thinking skills in Indonesia are still worrying. Based on that, this study aims to understand aspects of higher order thinking and describe its application in the content contained in textbooks in schools. In analyzing and describing content that has high-level thinking content, (1) cognitive level, (2) stimulus elements, and (3) contextual elements will be analyzed. This research method uses descriptive analytic method with a qualitative approach. The techniques used in this research are documents and notes. The text book used in this study is the Simpay Basa Sunda book at the VIII grade junior high school level. The results of this study contained 413 data whose details were (1) various cognitive levels in the book there were 72.39% at level 1 (knowledge and understanding), 9.68% at level 2 (application), and 17.91% at level 3 (reasoning). (2) the stimulus elements in this book are dominated by verbal stimuli totaling 323 stimuli, then visual stimuli totaling 1 stimulus. (3) there are 114 contextual elements in this book with a percentage of 28%. It can be concluded that this book can support the development of higher-order thinking skills, and can also support the ongoing 21st century learning process. However, it must be noted that this can be developed further by increasing the content containing HOTS.