Purpose: To compare the measurement of the amplitude of accommodation (AA) in different room illumination while using visual display unit (VDU) as a target. Method: A non-randomized, cross-sectional study includes 32 Malaysians aged between 15 to 35 years despite races and gender. Convenience sampling method was used. The data were analyzed by using one-way repeated measure ANOVA, to investigate the changes in the amplitude of accommodation in a different level of illuminations (23 Lux, 17 Lux, and 4 Lux). Result: 32 subjects’ data were analyzed. The amplitude of accommodation measured via a minus-lens method. No significant change has been observed among three levels of room illumination (p>0.05) with the mean difference of 0.222DS from the first room illumination to third room illumination. Furthermore, the association of AA among genders, age groups, race and refractive error for different levels of room illumination was not significant (p>0.05). However, an amplitude of accommodation between 15-21years and 22-28 years showed significant (p=0.047) difference in the first level of room illumination. Conclusion: The level of illumination has no clinically significant effect on the amplitude of accommodation. Key Words: Amplitude of Accommodation, room illumination, visual display unit (VDU), minus lens method
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