Visual Markers Research Articles

Warum besitzt die koptische Schrift das Zeichen ϯ? Ein einsames Syllabogramm sakralisiert die Schrift

“Why does the Coptic script have the character ϯ? A lone syllabogram sacralizes the script” – The Coptic character ϯ has several abnormalities within the Coptic script. On the one hand, it is the only syllabogram in the Coptic alphabet. On the other hand, it is not existent in all Coptic dialects and not at all in Old Coptic. Thus, the older explanation that this character did not evolve from a Demotic origin but is a ligature consisting of ⲧ and ⲓ should again be considered. In a second step, it is asked why Coptic has the character ϯ at all. There is hardly any evidence for phonetic reasons, and it is also not convincing to stress a better economy of writing provided by just one sign. Therefore, another explanation is proposed: In Late Antiquity, when the Coptic script emerged, mysticism of signs and numbers (Gematria) was a common exegetic strategy and cultural phenomenon. In this context, the letters ⲧ and ⲓ were, among others, heavily charged with supplementary meaning and also took part in the creation of monograms (like the Staurogram ⳨) and the special writing of nomina sacra. Furthermore, a strong cult of the cross has evolved since Constantine I. and has led to many visual and textual representations of the cross, which the sign ϯ obviously resembles. Thus the implementation of the otherwise not required ϯ can be seen as a sacralization, if not Christianization, of the Coptic Script whose progenitors were used in pagan or at least non-Christian contexts. Therefore, the rendering of the Egyptian-Coptic language by the Coptic alphabet, which consists of some extra characters originating from the Demotic script besides ϯ, became acceptable for Christian texts, although the Demotic script at this time almost exclusively occurs in pagan contexts. Although ϯ has primarily phonographic functions in most cases, it can serve as a visual marker of Christian creed. Thus, this contribution tries to connect Graphematics, Coptology, Patrology and Early Christian Art for a more holistic approach towards the understanding of the character ϯ.

Medium Depth Influences O2 Availability and Metabolism in Human RPE Cultures.

Retinal pigment epithelium (RPE) oxidative metabolism is critical for normal retinal function and is often studied in cell culture systems. Here, we show that conventional culture media volumes dramatically impact O2 availability, limiting oxidative metabolism. We suggest optimal conditions to ensure cultured RPE is in a normoxic environment permissive to oxidative metabolism. We altered the availability of O2 to human primary and induced pluripotent stem cell-derived RPE cultures directly via a hypoxia chamber or indirectly via the amount of medium over cells. We measured oxygen consumption rates (OCRs), glucose consumption, lactate production, 13C6-glucose and 13C5-glutamine flux, hypoxia inducible factor 1α (HIF-1α) stability, intracellular lipid droplets after a lipid challenge, transepithelial electrical resistance, cell morphology, and pigmentation. Medium volumes commonly employed during RPE culture limit diffusion of O2 to cells, triggering hypoxia, activating HIF-1α, limiting OCR, and dramatically altering cell metabolism, with only minor effects on typical markers of RPE health. Media volume effects on O2 availability decrease acetyl-CoA utilization, increase glycolysis and reductive carboxylation, and alter the size and number of intracellular lipid droplets under lipid-rich conditions. Despite having little impact on visible and typical markers of RPE culture health, media volume dramatically affects RPE physiology "under the hood." As RPE-centric diseases like age-related macular degeneration involve oxidative metabolism, RPE cultures need to be optimized to study such diseases. We provide guidelines for optimal RPE culture volumes that balance ample nutrient availability from larger media volumes with adequate O2 availability seen with smaller media volumes.

Epigenetics insights from perceived facial aging

Facial aging is the most visible manifestation of aging. People desire to look younger than others of the same chronological age. Hence, perceived age is often used as a visible marker of aging, while biological age, often estimated by methylation markers, is used as an objective measure of age. Multiple epigenetics-based clocks have been developed for accurate estimation of general biological age and the age of specific organs, including the skin. However, it is not clear whether the epigenetic biomarkers (CpGs) used in these clocks are drivers of aging processes or consequences of aging. In this proof-of-concept study, we integrate data from GWAS on perceived facial aging and EWAS on CpGs measured in blood. By running EW Mendelian randomization, we identify hundreds of putative CpGs that are potentially causal to perceived facial aging with similar numbers of damaging markers that causally drive or accelerate facial aging and protective methylation markers that causally slow down or protect from aging. We further demonstrate that while candidate causal CpGs have little overlap with known epigenetics-based clocks, they affect genes or proteins with known functions in skin aging, such as skin pigmentation, elastin, and collagen levels. Overall, our results suggest that blood methylation markers reflect facial aging processes, and thus can be used to quantify skin aging and develop anti-aging solutions that target the root causes of aging.

Meso-CF3-Difluoroboradiazaindacenes (BODIPY) as Markers for Visualization of Lysosomes

meso-CF3-Difluoroboradiazaindacenes (BODIPY) as Markers for Visualization of Lysosomes

VISUALITAS PENANDA DOMINANSI KONTEKS SIBERTEKS DALAM CYBERPRAGMATICS

Linguistic-pragmatic studies in conjunction with information technology has given rise to a new discipline in pragmatics called internet pragmatics or cyber-pragmatics. In this new pragmatics discipline, the speaker's intent cannot only be interpreted by replacing the conventional extra-linguistic context because the elements of that context are no longer sufficient to be used as a means of analysis and interpretation of intent. The dimensions of visual context as a marker of the dominance of cybertext context elements cannot be ignored in understanding the meaning of cybertext data. This cyber-pragmatics research aims to describe the manifestations of the visual dimensions that mark the dominance of cybertext context elements. The formulation of the main problem of this research is presented as follows: How is the manifestation of the visual dimensions marking the dominance of cybertext context elements in the cyber-pragmatics perspective? This study uses a phenomenological approach with a qualitative descriptive method. The target object of the research is the forms and functions that mark the visuality of the cybertext context. Furthermore, the research data is in the form of snippets of texts on the internet in which the target object of the research is contained, namely the form and function of cybertext context visual markers. Sources of substantive data for this research are social media texts in which there is research data, namely the form and function of visual markers in the cybertext context. Taking into account various limitations, research data is limited to a maximum of 9 snippets of cybertext from social media Instagram which are accessed around the time of the research. Data collection was carried out by applying the listening method to cybertext texts. The technique used is the read-note technique. With the methods and techniques of data collection, this research data is well identified for further classification. The data classification step is followed by the step of compiling data types so that they are ready for analysis. Data analysis was carried out by applying the extralingual equivalent method. The analysis technique used is the comparison technique. The analysis is carried out on data that has been properly typed so that interpretation is carried out easily. The next step is to present the results of the analysis and interpretation of the data using informal methods through ordinary language, not by interpreting symbols to convey certain rules. The results of this study are in the form of findings of visual manifestations in the multimodality cybertext context which are detailed as follows: (a) Visual Manifestations in the Form of Graphic Variations; (b) Visual Manifestation in the Form of Image Variations; (c) Visual Manifestations in the Form of Color Variations; (d) Visual Manifestations in the Form of Figure Variations; and (e) Visual Manifestations in the Form of Variations in Forms.

Norms that matter: Exploring the distribution of women’s work between income generation, expenditure-saving and unpaid domestic responsibilities in India

Based on primary data from a large household survey in seven districts in West Bengal in India, this paper analyses the reasons underlying low labor force participation of women. In developing countries, women who are engaged in unpaid economic work in family enterprises are often not counted as workers, whereas the men are. We show that for women, not being in paid work is not synonymous with not being in the labour force. Women are often involved in expenditure saving activities i.e. productive work within the family, over and above domestic chores and care work. We document the fuzziness of the boundary between domestic work and unpaid (and therefore invisible) productive work that leads to mismeasurement of women’s work and suggest methods to improve measurement. Counting women’s expenditure-saving activities yields a substantially higher estimate of women’s participation in economic work. On social norms, we show that religion and visible markers such as veiling are not significant determinants of the probability of being in paid work. We find that being primarily responsible for domestic chores lowers the probability of “working”, after accounting for all the conventional factors. Our data shows substantial unmet demand for paid work. Given that women are primarily responsible for domestic chores, we find that women express a demand for work that would be compatible with household chores. We demonstrate the existence of ‘virtuous cycles’ within families: a history of working women in the family (mother or mother-in-law ever worked) increases the probability of being in paid work between 18 and 21 percentage points. This suggests that the positive effects of increasing women’s labour force participation today are likely to have positive multiplier effects on the prospects for work in future generations of women.

Dissociable online integration processes in visual working memory.

Visual working memory has severe capacity limits, creating a bottleneck for active processing. A key way of mitigating this limitation is by chunking, i.e. compressing several pieces of information into one visual working memory representation. However, despite decades of research, chunking efficiency remains debated because of mixed evidence. We propose that there are actually 2 integration mechanisms: Grouping combines several objects to one representation, and object-unification merges the parts of a single object. Critically, we argue that the fundamental distinction between the 2 processes is their differential use of the pointer system, the indexing process connecting visual working memory representations with perception. In grouping, the objects that are represented together still maintain independent pointers, making integration costly but highly flexible. Conversely, object-unification fuses the pointers as well as the representations, with the single pointer producing highly efficient integration but blocking direct access to individual parts. We manipulated integration cues via task-irrelevant movement, and monitored visual working memory's online electrophysiological marker. Uniquely colored objects were flexibly grouped and ungrouped via independent pointers (experiment 1). If objects turned uniformly black, object-integration could not be undone (experiment 2), requiring visual working memory to reset before re-individuation. This demonstrates 2 integration levels (representational-merging versus pointer-compression) and establishes the dissociation between visual working memory representations and their underlying pointers.

Stakeholder Challenges and Opportunities of GPS Shock Collars to Achieve Optimum Welfare in a Conservation or Farm Setting.

Virtual fences for livestock facilitated by a GPS shock collar (GPS-SC) and phone app were introduced to the UK in cattle herd trials in 2020. Technology which uses aversive shocks to control livestock movement on farms and in other settings poses a significant risk to livestock welfare. There are currently no welfare protocols in place in the UK to ensure the ethical use of GPS-SCs. The objective of this study was to understand how GPS-SCs were being used in practice in the UK and gather data to assist researchers and policymakers in the future research and development of a welfare protocol for the UK. We studied how the technology performs in terms of welfare challenges and opportunities, covering extensive livestock production, conservation settings, "rewilding", and regenerative farming practices, where the technology is currently being applied. Semistructured interviews were conducted with key stakeholders. In-depth interviews (n = 8) supported the previous literature that the use of GPS-SCs in restricted grazing settings poses a risk to animal welfare. This is due to the wavering virtual fence boundary line (which is affected by satellite movements), a lack of visual markers, and, in some "rewilding" and conservation settings, livestock keepers, which require training and support to enable optimal welfare in practice and prevent misuse of the technology. Results also indicated that there are opportunities for enhancing livestock welfare with GPS-SCs in very extensive farm settings, where targeted care can be facilitated by using the data to monitor and track livestock using GPS-SCs, and which can also prevent cattle injury or fatality through virtual pastures designed to protect livestock from hazards such as roads or bogs. Future research is needed to focus on minimising shocks in the training period and to better understand the value of visual electric fences in the training process.

Testing a candidate meiotic drive locus identified by pool sequencing.

Meiotic drive biases the transmission of alleles in heterozygous individuals, such that Mendel's law of equal segregation is violated. Most examples of meiotic drive have been discovered over the past century based on causing sex ratio distortion or the biased transmission of easily scoreable genetic markers that were linked to drive alleles. More recently, several approaches have been developed that attempt to identify distortions of Mendelian segregation genome wide. Here, we test a candidate female meiotic drive locus in Drosophila melanogaster, identified previously as causing a ∼54:46 distortion ratio using sequencing of large pools of backcross progeny. We inserted fluorescent visible markers near the candidate locus and scored transmission in thousands of individual progeny. We observed a small but significant deviation from the Mendelian expectation; however, it was in the opposite direction to that predicted based on the original experiments. We discuss several possible causes of the discrepancy between the 2 approaches, noting that subtle viability effects are particularly challenging to disentangle from potential small-effect meiotic drive loci. We conclude that pool sequencing approaches remain a powerful method to identify candidate meiotic drive loci but that genotyping of individual progeny at early developmental stages may be required for robust confirmation.

In vivo direct cell-penetrating peptide mediated protein transduction system in Acyrthosiphon pisum

ObjectiveThe principal delivery method for CRISPR-based genome editing in insects is now based on microinjection into single cells or embryos. The direct protein transduction systems cannot be employed in aphids because oogenesis occurs without apparent vitellogenesis. Given the limited timing of injection into the embryonic stage in oviparous aphids, a protein delivery system from the hemolymph to the germline and embryos would be a useful tool for genome editing. This study reports a newly developed direct protein delivery system for aphids using cell-penetrating peptides (CPPs). CPPs are short peptides that translocate across the plasma membrane when bound to cargo proteins.ResultsPenetratin (PEN), a widely conserved CPP among insects, was identified in this study. We used mVenus, a recombinant fluorescent protein, as a visual marker for CPP availability assessments, and fused it with PEN by bacterial protein expression. The mVenus-PEN recombinant proteins were introduced into the hemolymph of adult unwinged Acyrthosiphon pisum females using a nanoinjector. Fluorescence emitted by mVenus-PEN was observed in various tissues, such as the gut, trachea, bacteriocytes, and their progeny. This study shows that PEN can deliver exogenously expressed proteins into tissues in vivo, indicating that CPPs are powerful tools for protein transduction.

Mechanical Dyssynchrony Combined with Septal Scarring Reliably Identifies Responders to Cardiac Resynchronization Therapy.

Background and aim: The presence of mechanical dyssynchrony on echocardiography is associated with reverse remodelling and decreased mortality after cardiac resynchronization therapy (CRT). Contrarily, myocardial scar reduces the effect of CRT. This study investigated how well a combined assessment of different markers of mechanical dyssynchrony and scarring identifies CRT responders. Methods: In a prospective multicentre study of 170 CRT recipients, septal flash (SF), apical rocking (ApRock), systolic stretch index (SSI), and lateral-to-septal (LW-S) work differences were assessed using echocardiography. Myocardial scarring was quantified using cardiac magnetic resonance imaging (CMR) or excluded based on a coronary angiogram and clinical history. The primary endpoint was a CRT response, defined as a ≥15% reduction in LV end-systolic volume 12 months after implantation. The secondary endpoint was time-to-death. Results: The combined assessment of mechanical dyssynchrony and septal scarring showed AUCs ranging between 0.81 (95%CI: 0.74-0.88) and 0.86 (95%CI: 0.79-0.91) for predicting a CRT response, without significant differences between the markers, but significantly higher than mechanical dyssynchrony alone. QRS morphology, QRS duration, and LV ejection fraction were not superior in their prediction. Predictive power was similar in the subgroups of patients with ischemic cardiomyopathy. The combined assessments significantly predicted all-cause mortality at 44 ± 13 months after CRT with a hazard ratio ranging from 0.28 (95%CI: 0.12-0.67) to 0.20 (95%CI: 0.08-0.49). Conclusions: The combined assessment of mechanical dyssynchrony and septal scarring identified CRT responders with high predictive power. Both visual and quantitative markers were highly feasible and demonstrated similar results. This work demonstrates the value of imaging LV mechanics and scarring in CRT candidates, which can already be achieved in a clinical routine.

Engineering of insecticidal hybrid gene into potato chloroplast genome exhibits promising control of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae).

The potato chloroplast was transformed with codon optimized synthetic hybrid cry gene (SN19) to mitigate crop losses by Colorado potato beetle (CPB). The bombarded explants (leaves and internode) were cultured on MS medium supplemented with BAP (2.0mg/l), NAA (0.2mg/l), TDZ (2.0mg/l) and GA3 (0.1mg/l); spectinomycin 50mg/l was used as a selection agent in the medium. Leaf explants of cultivar Kuroda induced highest percentage (92%) of callus where cultivar Santae produced the highest percentage (85.7%) of transplastomic shoots. Sante and Challenger showed 9.6% shoot regeneration efficiency followed by cultivar Simply Red (8.8%). PCR amplification yielded 16 postive transplastomic plantlets out of 21 spectinomycin resistant ones. Target gene integration was confirmed by PCR and Southern blot, whereas RT-qPCR was used to assess the expression level of transgene. The localization of visual marker gene gfp was tracked by laser scanning confocal microscopy which confirmed its expression in chloroplasts of leaf cells. The transplastomic plants ensured high mortality to both larvae and adult CPB. Foliage consumption and weight gain of CPB fed on transplastomic leaves were lower compared to the control plants. Sucessful implementation of current research findings can lead to a viable solution to CPB mediated potato losses globally.

Screening Mosquito Larvae Under a Fluorescence Binocular Microscope.

Transgenic mosquitoes are used in many aspects of mosquito research, and convenient selection markers are crucial to identifying transgenic individuals even among large numbers of wild types. Visual markers, in the form of fluorescent proteins expressed in larval and adult mosquito tissues, are the most commonly used. This requires observing large numbers of mosquitoes under the fluorescence microscope and recognizing positive individuals expressing the fluorescent genetic marker. As research models, mosquito larvae possess the following two advantages over many other insects, greatly facilitating fluorescence screening: (1) Being aquatic, they can be isolated in a drop of clear water, an ideal medium for live observations under the binocular microscope; and (2) synchronous hatching from many eggs is easily obtained, so that large populations of larvae can be screened in batches of several tens of thousands at a time. Screening at the neonate stage, when larvae are ∼1-mm-long, allows the concentration of hundreds of larvae in a drop of water that fits in the observation field of the microscope at medium magnification. Thus, many individuals can be screened rapidly. We strongly recommend screening larvae at the neonate stage and before any feeding for two reasons, as follows: (1) Food particles can be strongly autofluorescent, thereby dramatically increasing the fluorescence background noise around larvae, and (2) tissue autofluorescence increases during development, notably in the digestive tract, significantly decreasing the specific signal-to-noise ratio. In this protocol, we guide the experimenter step-by-step for a fast and efficient medium-throughput manual screening for fluorescent larvae.

Perspectives on Secondary Mitral Regurgitation in Heart Failure.

This review focuses on broader perspectives of mitral regurgitation (MR) in patients with heart failure. The ratio of regurgitant volume to end-diastolic volume appears to help identify patients who may benefit from valve interventions. Secondary MR is not only attributed to geometric changes of the LV but also related to the structural changes in the mitral valve that include fibrosis of the mitral leaflets and changes in the extracellular matrix. The transition from mild to severe secondary MR can occur at different rates, from a slow LV remodeling process to a more abrupt process precipitated by an inciting event such as atrial fibrillation. Septal flash and apical rocking, two new visual markers of LV mechanical dyssynchrony, appear to be predictive of MR reduction following cardiac resynchronization therapy. Optimal guideline-directed medical therapy has been shown to decrease the severity of secondary MR effectively. A theoretical framework to characterize secondary MR as it relates to the onset of MR is proposed. Type A: Early onset of MR contemporaneous with myocardial injury. The maladaptive LV remodeling occurs in parallel with MR. Type B: LV remodeling proceeds without significant MR until the LV is moderately dilated, which coincides with or without inciting factors such as atrial fibrillation. Type C: LV remodeling proceeds after myocardial injury without significant MR until the LV is severely dilated. MR is a late manifestation of LV remodeling.

Machine Learning of Functional Connectivity to Biotype Alcohol and Nicotine Use Disorders

BackgroundMagnetic resonance imaging provides noninvasive tools to investigate alcohol use disorder (AUD) and nicotine use disorder (NUD) and neural phenotypes for genetic studies. A data-driven transdiagnostic approach could provide a new perspective on the neurobiology of AUD and NUD. MethodsUsing samples of individuals with AUD (n = 140), individuals with NUD (n = 249), and healthy control participants (n = 461) from the UK Biobank, we integrated clinical, neuroimaging, and genetic markers to identify biotypes of AUD and NUD. We partitioned participants with AUD and NUD based on resting-state functional connectivity (FC) features associated with clinical metrics. A multitask artificial neural network was trained to evaluate the cluster-defined biotypes and jointly infer AUD and NUD diagnoses. ResultsThree biotypes—primary NUD, mixed NUD/AUD with depression and anxiety, and mixed AUD/NUD—were identified. Multitask classifiers incorporating biotype knowledge achieved higher area under the curve (AUD: 0.76, NUD: 0.74) than single-task classifiers without biotype differentiation (AUD: 0.61, NUD: 0.64). Cerebellar FC features were important in distinguishing the 3 biotypes. The biotype of mixed NUD/AUD with depression and anxiety demonstrated the largest number of FC features (n = 5), all related to the visual cortex, that significantly differed from healthy control participants and were validated in a replication sample (p < .05). A polymorphism in TNRC6A was associated with the mixed AUD/NUD biotype in both the discovery (p = 7.3 × 10−5) and replication (p = 4.2 × 10−2) sets. ConclusionsBiotyping and multitask learning using FC features can characterize the clinical and genetic profiles of AUD and NUD and help identify cerebellar and visual circuit markers to differentiate the AUD/NUD group from the healthy control group. These markers support a new growing body of literature.

Rapid generation of a tomato male sterility system and its feasible application in hybrid seed production.

A practical approach for the rapid generation and feasible application of green hypocotyl male-sterile (GHMS) tm6 dfr lines in tomato hybrid breeding was established. Male sterility enables reduced cost and high seed purity during hybrid seed production. However, progress toward its commercial application has been slow in tomato due to the disadvantages of most natural male-sterile mutants. Here, we developed a practical method for efficient tomato hybrid seed production using a male-sterile system with visible marker, which was rapidly generated by CRISPR/Cas9-mediated gene editing. Two closely linked genes, TM6 and DFR, which were reported to be candidates of ms15 (male sterile-15) and aw (anthocyanin without) locus, respectively, were knocked out simultaneously in two elite tomato inbred lines. Mutagenesis of both genes generated green hypocotyl male-sterile (GHMS) lines. The GHMS lines exhibited male sterility across different genetic backgrounds and environmental conditions. They also showed green hypocotyl due to defective anthocyanin accumulation, which serves as a reliable visible marker for selecting male-sterile plants at the seedling stage. We further proposed a strategy for multiplying the GHMS system and verified its high efficiency in stable male sterility propagation. Moreover, elite hybrid seeds were produced using GHMS system for potential side effects evaluation, and no adverse influences were found on seed yield, seed quality as well as important agronomic traits. This study provides a practical approach for the rapid generation and feasible application of male sterility in tomato hybrid breeding.

A randomized control trial to support smoke-free policy compliance in public housing

BackgroundSmoke-free housing policies in multiunit housing are increasingly widespread interventions to reduce smoking and secondhand smoke exposure. Little research has identified factors that impede compliance with smoke-free housing policies in low-income multiunit housing and test corresponding solutions.MethodsWe are using an experimental design to test two compliance support interventions: (A) a “compliance through reduction (via relocation and reduction in personal smoking) and cessation” intervention targets households with smokers and involves support to shift smoking practices to areas beyond the apartment or building setting, reduce personal smoking, and deliver in-residence smoking cessation support services via trained peer educators and (B) a “compliance through resident endorsement” intervention involving voluntary adoption of smoke-free living environments through personal pledges, visible door markers, and/or via social media. We will compare randomly sampled participants in buildings that receive A or B or A plus B to the NYCHA standard approach.DiscussionThis RCT addresses key gaps in knowledge and capitalizes on key scientific opportunities by (1) leveraging the federal mandate to ban smoking in a public housing system of more than sufficient size to conduct an adequately powered RCT; (2) expanding our understanding of smoke-free policy compliance beyond policy implementation by testing two novel treatments: (a) in-residence smoking cessation and (b) resident endorsement, while (3) addressing population and location-specific tobacco-related disparities. At the conclusion of the study, this RCT will have leveraged a monumental policy shift affecting nearly half a million NYC public housing residents, many of whom disproportionately experience chronic illness and are more likely to smoke and be exposed to secondhand smoke than other city residents. This first-ever RCT will test the effects of much-needed compliance strategies on resident smoking behavior and secondhand smoke exposure in multiunit housing.Trial registrationClinical Trials Registered, NCT05016505. Registered on August 23, 2021.

Radiomic markers of intracerebral hemorrhage expansion on non-contrast CT: independent validation and comparison with visual markers.

To devise and validate radiomic signatures of impending hematoma expansion (HE) based on admission non-contrast head computed tomography (CT) of patients with intracerebral hemorrhage (ICH). Utilizing a large multicentric clinical trial dataset of hypertensive patients with spontaneous supratentorial ICH, we developed signatures predictive of HE in a discovery cohort (n = 449) and confirmed their performance in an independent validation cohort (n = 448). In addition to n = 1,130 radiomic features, n = 6 clinical variables associated with HE, n = 8 previously defined visual markers of HE, the BAT score, and combinations thereof served as candidate variable sets for signatures. The area under the receiver operating characteristic curve (AUC) quantified signatures' performance. A signature combining select radiomic features and clinical variables attained the highest AUC (95% confidence interval) of 0.67 (0.61-0.72) and 0.64 (0.59-0.70) in the discovery and independent validation cohort, respectively, significantly outperforming the clinical (pdiscovery = 0.02, pvalidation = 0.01) and visual signature (pdiscovery = 0.03, pvalidation = 0.01) as well as the BAT score (pdiscovery < 0.001, pvalidation < 0.001). Adding visual markers to radiomic features failed to improve prediction performance. All signatures were significantly (p < 0.001) correlated with functional outcome at 3-months, underlining their prognostic relevance. Radiomic features of ICH on admission non-contrast head CT can predict impending HE with stable generalizability; and combining radiomic with clinical predictors yielded the highest predictive value. By enabling selective anti-expansion treatment of patients at elevated risk of HE in future clinical trials, the proposed markers may increase therapeutic efficacy, and ultimately improve outcomes.

A 3D pose estimation framework for preterm infants hospitalized in the Neonatal Unit

AbstractInfant pose estimation is crucial in different clinical applications, including preterm automatic general movements assessment. Recent infant pose estimation methods are limited by a lack of real clinical data and are mainly focused on 2D detection. We introduce a stereoscopic system for infants’ 3D pose estimation, based on fine-tuning state-of-the-art 2D human pose estimation networks on a large, real, and manually annotated dataset of infants’ images. Our dataset contains over 88k images, collected from 175 videos from 53 premature infants born &lt;33 weeks of gestational age (GA), acquired within the Neonatology department of the Centre Hospitalier Universitaire de Saint Etienne, France, between 32 and 41 weeks of GA. This framework significantly reduced the pose estimation error compared to existing 2D infant pose estimation networks. It achieved a mean error of 1.72 cm on 18000 stereoscopic images in the 3D pose estimation task. This framework is the first 3D pose estimation tool dedicated to preterm infants hospitalized in the Neonatal Unit that does not depend on any visual markers or infrared cameras.

Engineering a complex, multiple enzyme-mediated synthesis of natural plant pigments in the silkworm, Bombyx mori

Plants produce various pigments that not only appear as attractive colors but also provide valuable resources in applications in daily life and scientific research. Biosynthesis pathways for these natural plant pigments are well studied, and most have multiple enzymes that vary among plant species. However, adapting these pathways to animals remains a challenge. Here, we describe successful biosynthesis of betalains, water-soluble pigments found only in a single plant order, Caryophyllales, in transgenic silkworms by coexpressing three betalain synthesis genes, cytochrome P450 enzyme CYP76AD1, DOPA 4,5-dioxygenase, and betanidin 5-O-glucosyltransferase. Betalains can be synthesized in various tissues under the control of the ubiquitous IE1 promoter but accumulate mainly in the hemolymph with yields as high as 274 μg/ml. Additionally, transformed larvae and pupae show a strong red color easily distinguishable from wild-type animals. In experiments in which expression is controlled by the promoter of silk gland-specific gene, fibroin heavy-chain, betalains are found predominantly in the silk glands and can be secreted into cocoons through spinning. Betalains in transformed cocoons are easily recovered from cocoon shells in water with average yields reaching 14.4 μg/mg. These data provide evidence that insects can synthesize natural plant pigments through a complex, multiple enzyme-mediated synthesis pathway. Such pigments also can serve as dominant visible markers in insect transgenesis applications. This study provides an approach to producing valuable plant-derived compounds by using genetically engineered silkworms as a bioreactor.