In this study, the effect of variation of the grading of fine and coarse aggregate within the range specified in JIS on the fluidity, viscosity and segregation resistance of fresh concrete was investigated. Additionally, the effect was considered based on the excess paste theory. The following findings were obtained as the results. (1) The variation of grading of fine aggregate has a great effect on the fluidity and the segregation resistance of concrete. On the other hand, that of coarse aggregate has a relatively small effect on these properties. (2) The effects mentioned above can be theoretically controlled based on the distance between each aggregate in dispersion. (3) The fluidity of concrete is dominantly affected by the properties of aggregate such as grading. On the other hand, the viscosity of concrete is greatly affected by the properties of cement paste.