Virus In Ghana Research Articles

Antibiotic resistance and draft genome profiles of 10 Streptococcus pneumoniae and 3 Streptococcus pseudopneumoniae strains isolated from the nasopharynx of people living with human immunodeficiency virus in Ghana.

Genomic data on clinically important bacteria species such as Streptococcus pneumoniae and Streptococcus pseudopneumoniae from low- and middle-income countries, including Ghana, are scarce. In this study, we provide data on antimicrobial resistance (AMR) and whole-genome profiles of a collection of streptococci species to support AMR surveillance efforts in the country.

Tetracistronic minigenomes elucidate a functional promoter for Ghana virus and unveils Cedar virus replicase promiscuity for all henipaviruses.

Henipaviruses are recognized as significant global health threats due to their high mortality rates and lack of effective vaccines or therapeutics. Due to the requirement for high biocontainment facilities, the scope of research which may be conducted on henipaviruses is limited. To address this challenge, we developed innovative tetracistronic, transcription, and replication-competent minigenomes. We demonstrate that these systems replicate key aspects of the viral life cycle, such as budding, fusion, and receptor binding, and are safe for use in lower biocontainment settings. Importantly, the application of this system to the Ghana virus revealed that its known sequence is incomplete; however, substituting the missing sequences with those from other henipaviruses allowed us to overcome this challenge. We demonstrate that the Ghana virus replicative machinery is functional and can identify two orally efficacious antivirals effective against it. Our research offers a versatile system for life-cycle modeling of highly pathogenic henipaviruses at low biocontainment.

A Retrospective Analysis Reveals That the 2021 Outbreaks of African Swine Fever Virus in Ghana Were Caused by Two Distinct Genotypes.

African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), a highly infectious and lethal disease of domesticated swine. Outbreaks of ASF have been mostly restricted to the continent of Africa. The outbreaks that have occurred outside of Africa were controlled by extensive depopulation of the domesticated pig population. However, in 2007, an outbreak occurred in the country of Georgia, where ASFV infected wild pigs and quickly spread across eastern Europe. Since the reintroduction of ASF into Europe, variants of the current pandemic strain, ASFV Georgia 2007/01 (ASFV-G), which is classified as Genotype 2 based on p72 sequencing, have been reported in countries within western Europe, Asia, and the island of Hispaniola. Additionally, isolates collected in 2020 confirmed the presence of variants of ASFV-G in Nigeria. Recently, we reported similar variants of ASFV-G collected from domestic pigs suspected of dying of ASF in Ghana in 2022. Here, we retroactively report, based on full-length sequencing, that similar variants were present in Ghana in 2021. The SNP analysis revealed derivatives of ASFV with distinct genetic markers. Furthermore, we identified three full-length ASFV genomes as Genotype 1, indicating that there were two genotypes circulating in proximity during the 2021 ASF outbreaks in Ghana.

Structure-guided mutagenesis of Henipavirus receptor-binding proteins reveals molecular determinants of receptor usage and antibody-binding epitopes.

Hendra virus and Nipah virus (NiV) are lethal, zoonotic Henipaviruses (HNVs) that cause respiratory and neurological clinical features in humans. Since their initial outbreaks in the 1990s, several novel HNVs have been discovered worldwide, including Ghana virus. Additionally, there is serological evidence of zoonotic transmission, lending way to concerns about future outbreaks. HNV infection of cells is mediated by the receptor-binding protein (RBP) and the Fusion protein (F). The work presented here identifies NiV RBP amino acids important for the usage of ephrin-B3 (EFNB3), a receptor highly expressed in neurons and predicted to be important for neurological clinical features caused by NiV. This study also characterizes epitopes recognized by antibodies against divergent HNV RBPs. Together, this sheds insight to amino acids critical for HNV receptor usage and antibody binding, which is valuable for future studies investigating determinants of viral pathogenesis and developing antibody therapies.

Cacao Swollen Shoot Viruses in Ghana.

Cacao swollen shoot virus causes cacao swollen shoot disease of Theobroma cacao (cacao) plants. At least six cacao-infecting Badnavirus species-Cacao swollen shoot Togo A virus, Cacao swollen shoot Togo B virus (previously known as Cacao swollen shoot virus), Cacao swollen shoot CE virus, Cacao swollen shoot Ghana M virus, Cacao swollen shoot Ghana N virus, and Cacao swollen shoot Ghana Q virus-are responsible for the swollen shoot disease of cacao in Ghana. Each of these species consists of a multiplicity of strains. The New Juaben strain, the most virulent cacao swollen shoot virus strain in Ghana, belongs to the Cacao swollen shoot Togo B virus species, and is a commonly used strain in laboratory transmission assays. Infection of cacao trees with multiple strains of the virus is common and new evidence suggests that these coinfections may have resulted in the emergence of recombinant strains of the virus. The impact of these emerging recombinant strains on disease severity is uncertain. This review focuses largely on the discovery of cacao swollen shoot virus in Ghana, diversity of the virus strains, molecular characterization, propagation of virus infection in cacao plants, emergence of recombinant virus strains, vector-mediated transmission of the virus, and the management of the cacao swollen shoot disease in Ghana. It also contains sections on the botany and origin of the cacao tree, its introduction to Ghana, the role of cacao swollen shoot disease in facilitating Ghana's independence from Britain, and a brief history of chocolate.

Genetic diversity and epidemic histories of rice yellow mottle virus in Ghana

Rice yellow mottle virus (RYMV) has persisted as a major biotic constraint to rice production in Africa. However, no data on RYMV epidemics were available in Ghana, although it is an intensive rice-producing country. Surveys were performed from 2010 to 2020 in eleven rice-growing regions of Ghana. Symptom observations and serological detections confirmed that RYMV is circulating in most of these regions. Coat protein gene and complete genome sequencings revealed that RYMV in Ghana almost exclusively belongs to the strain S2, one of the strains covering the largest area in West Africa. We also detected the presence of the S1ca strain which is being reported for the first time outside its area of origin. These results suggested a complex epidemiological history of RYMV in Ghana and a recent expansion of S1ca to West Africa. Phylogeographic analyses reconstructed at least five independent RYMV introductions in Ghana for the last 40 years, probably due to rice cultivation intensification in West Africa leading to a better circulation of RYMV. In addition to identifying some routes of RYMV dispersion in Ghana, this study contributes to the epidemiological surveillance of RYMV and helps to design disease management strategies, especially through breeding for rice disease resistance.

Deadly Marburg virus in Ghana, 2022 amidst monkeypox and COVID-19 pandemic: A distressing concern for public health.

Deadly Marburg virus in Ghana, 2022 amidst monkeypox and COVID-19 pandemic: A distressing concern for public health.

Recombinant Soluble Henipavirus Glycoprotein Preparation.

Henipaviruses possess two envelope glycoproteins, the attachment (G) and the fusion (F) proteins that mediate cellular entry and are the major targets of virus-neutralizing antibody responses. Recombinant expression technologies have been used to produce soluble G and F proteins (sG and sF) that retain native-like oligomeric conformations and epitopes, which are advantageous for the development and characterization of vaccines and antiviral antibody therapeutics. In addition to Hendra virus and Nipah virus tetrameric sG and trimeric sF production, we also describe the expression and purification of Cedar virus tetrameric sG and Ghana virus trimeric sF glycoproteins. These henipavirus glycoproteins were also used as immunizing antigens to generate monoclonal antibodies, and binding was demonstrated with a pan-henipavirus multiplex microsphere immunoassay.

Marburg virus in Ghana: A public health threat to Ghanaians and to Africans

AbstractThe Marburg virus disease (MVD) outbreak in Ghana and the re‐emergence of other infectious diseases in Africa require a unified effort to fight these highly infectious health threats. Although no new cases have been reported after the three MVD confirmed cases, Ghana Health Service (GHS), and Health Authorities of neighbouring countries are on the alert to identify any new cases and deal with them at the earliest. Public health measures, however, inadequate, are in place in Ghana. Varied challenges remain in the fight against these outbreaks. All known public health measures must be brought to bear in the fight. African governments must resource the African Centre for Diseases Control and Prevention, in its efforts to augment the various country‐level health services in the fight against the threats. African governments, citizens, development partners, and foreign governments must commit to fighting these diseases before they turn into pandemics.

Cryptococcal and Histoplasma Antigen Screening Among People With Human Immunodeficiency Virus in Ghana and Comparative Analysis of OIDx Histoplasma Lateral Flow Assay and IMMY Histoplasma Enzyme Immunoassay.

BackgroundCryptococcal meningitis (CM) and disseminated histoplasmosis (DH) are common in people with human immunodeficiency virus (PWH) and diagnosed by detecting cryptococcal antigen (CrAg) and Histoplasma antigen (HistoAg), respectively. In Ghana, CM and DH are rarely suspected by clinicians due to limited epidemiological data.MethodsThis study was conducted among PWH in Ghana who are unwell. Sociodemographic and clinical data were collected by questionnaire. Serum and/or urine were screened for CrAg and HistoAg, using IMMY CrAg lateral flow assay (LFA) and IMMY Histoplasma enzyme immunoassay (EIA) kits, respectively, regardless of symptoms. Samples run with IMMY Histoplasma EIA were simultaneously run with Optimum Imaging Diagnostics (OIDx) Histoplasma LFA. Laboratory investigations were conducted by the research team, and diagnosis incorporating clinical assessment, screening, and confirmatory testing results and treatment decisions were made by the clinical team. Treatment and outcome information on CM and DH patients were evaluated.ResultsOverall, 150 participants were recruited. There were 73% (n = 109) females, and the age range was 18–62 years. The prevalence rates of CrAg and HistoAg were 2.7% (4 of 150) and 4.7% (5 of 107), respectively. The OIDx Histoplasma LFA showed a high concordance (98.4%) with the IMMY Histoplasma EIA. All antigen-positive cases by standard tests were diagnosed with CM and DH. Antifungal treatment was given in 5 patients and follow-up revealed 2 deaths and 3 recoveries.ConclusionsHistoplasmosis among PWH may be more common than previously anticipated and may be more frequent than cryptococcosis in Ghana. The performance of the OIDx Histoplasma LFA should be further explored.

Impact of Personal Protective Equipment in the Covid-19 Era: A Study of Health Workers in Ghana

COVID-19 has become well-known in the world. The measures to kill the virus have also become hard to come by. Personal Protective Equipment (PPE) has been using by health workers to contain the virus in Ghana hence the study sought to ascertain the impact of Personal Protective Equipment on Ghanaian health workers in the COVID-19 era. The study used primary and secondary sources of data. Importantly, a simple random sampling technique was used to engage a total of 2,420 health workers in Ghana, and also convenience sampling technique was used to select 150 public hospitals and 150 private hospitals for the study. The study used both questionnaires and in-depth interviews to gather the required information. The information obtained from the participants was analyzed by content and statistical analysis. It was revealed that health workers have been using Personal Protective Equipment frequently to contain the spread of the COVID-19 virus in the health care setting in Ghana. It was recommended that health workers should use the Personal Protective Equipment according to indication. Intensive monitoring was also recommended to check the proper uses of the Personal Protective Equipment by health workers in Ghana. Lastly, a sensitization programme was recommended to give more insight on the importance of using Personal Protective Equipment by health workers in Ghana.

Inconsistent PCR detection of Cacao swollen shoot virus (CSSV) is linked to the occurrence of different variants across the cocoa regions of Ghana

Reliable diagnostic tools capable of detecting latent and asymptomatic infections are critically important to support the management of the cocoa swollen shoot virus disease (CSSVD) and also to complement research activities on screening for resistant cocoa varieties. Development of efficient polymerase chain reaction (PCR) assays sensitive for detection of CSSV infections has thus been a major research focus over the years. Advances in the full genome sequence information have resulted in the design of several Cacao swollen shoot virus (CSSV)-specific and degenerate primers. The objective of the present study was to further assess the detection efficiency of ten (10) of the most utilized and novel CSSV primers on isolates of the virus across Ghana. Results from the PCR assays showed a highly variable and poor efficiency of the primers on the 189 samples of CSSV isolates evaluated. The overall detection potential of the primers ranged from 4 % to 23 % with the four best performing primers in terms of PCR positivity being P4 (23 %), CSSD1 (21 %), JOEL (21 %) CSSD2 (19 %) and BADNA (19 %). The generally poor and inconsistent efficiency of the primers are discussed in the context of the genetic variability and also the occurrence of new variants of the virus in Ghana.

Chronicling Country-Specific Response to Covid-19 Pandemic in Africa: From the Perspective of Ghana

Health is closely tied to human development and the overall development of a nation. Indeed, throughout history, improved public health has been a major determinant of development. Within this context, the consequences of infectious diseases including the novel Coronavirus are not in doubt. Particularly, the COVID-19 pandemic has destabilized the much-talked about world civilization with many nations still under various infection control measures. Globally, the pandemic has resulted in several losses in the areas of jobs, income, government revenues, foreign direct investments, among others. Beyond the incalculable economic losses, the pandemic constitutes a great threat to physical, social and mental health. Since the outbreak of the pandemic, several studies have been conducted on the experiences of different countries to help deepen global knowledge on the pandemic. This review paper chronicles the Ghanaian experience of COVID-19 and attempts to probe whether the country’s overall response to the disease is worthy of emulation by other nations. Part one introduces the paper by looking at the intersection of health and development, a brief history of COVID-19, challenges associated with the pandemic and measures some countries have employed to rebuild their economies. Part two describes the key issues underpinning the spread of the virus in Ghana whilst part three considers the efforts key stakeholders employed to contain and manage the spread of the virus. Part four focuses on the challenges Ghana has faced in the management of the pandemic whereas the concluding part presents the core issues which should engage the attention of stakeholders. Keywords: COVID-19 pandemic, misinformation, social risk amplification and attenuation theory, health services, development DOI: 10.7176/NMMC/97-03 Publication date: August 31 st 2021

Functional Analysis of the Fusion and Attachment Glycoproteins of Mojiang Henipavirus.

Mojiang virus (MojV) is the first henipavirus identified in a rodent and known only by sequence data, whereas all other henipaviruses have been isolated from bats (Hendra virus, Nipah virus, Cedar virus) or discovered by sequence data from material of bat origin (Ghana virus). Ephrin-B2 and -B3 are entry receptors for Hendra and Nipah viruses, but Cedar virus can utilize human ephrin-B1, -B2, -A2 and -A5 and mouse ephrin-A1. However, the entry receptor for MojV remains unknown, and its species tropism is not well characterized. Here, we utilized recombinant full-length and soluble forms of the MojV fusion (F) and attachment (G) glycoproteins in membrane fusion and receptor tropism studies. MojV F and G were functionally competent and mediated cell–cell fusion in primate and rattine cells, albeit with low levels and slow fusion kinetics. Although a relative instability of the pre-fusion conformation of a soluble form of MojV F was observed, MojV F displayed significantly greater fusion activity when heterotypically paired with Ghana virus G. An exhaustive investigation of A- and B-class ephrins indicated that none serve as a primary receptor for MojV. The MojV cell fusion phenotype is therefore likely the result of receptor restriction rather than functional defects in recombinant MojV F and G glycoproteins.

Quality of antiretroviral therapy services in Ghana: Implications for the HIV response in resource-constrained settings.

Objective:Number of People Living with Human Immune-deficiency Virus in Ghana is over 300,000 and unmet need for antiretroviral therapy is approximately 60%. This study sought to determine the quality of antiretroviral therapy services in selected ART sites in Ghana using the input-process-outcome approach.Methods:This is a descriptive cross-sectional case study that employed modified normative evaluation to assess quality of antiretroviral therapy services in the Oti and Volta regions of Ghana among People Living with HIV (n = 384) and healthcare providers (n = 16). The study was conducted from 11 March to 9 May 2019.Results:Resources for managing HIV clients were largely available with the exception of viral load machines, reagents for CD4 counts, and antifungals such as Fluconazole and Cotrimoxazole. Patients enrolled on antiretroviral therapy within 2 weeks was 71% and clients retained in care within 2 weeks of enrolment was 90%. Approximately 26% of enrolled clients recorded viral load suppression; 33% of People Living with HIV who were not insured with the National Health Insurance Scheme paid for some antiretrovirals and cotrimoxazole. Adherence to ART and Cotrimoxazole were 95% and 88%, respectively, using pill count on their last three visits. Time spent with clinical team was among the worst rated (mean = 2.98, standard deviation = 0.54) quality indicators by patients contrary to interpersonal relationship with health provider which was among the best rated (mean = 3.25, standard deviation = 0.41) indicators.Conclusion:Observed quality care gaps could potentially reverse gains made in HIV prevention and control in Ghana if not addressed timely; an important value addition of this study is the novel application of input-process-outcome approach in the context of antiretroviral therapy services in Ghana. There is also the need for policy dialogue on inclusion of medications for prophylaxis in antiretroviral therapy on the National Health Insurance Scheme to promote adherence and retention.

Religion and the state in an episodic moment of COVID-19 in Ghana.

The goal of this paper is to discuss the role religions are playing to stem the tide against the spread of coronavirus (COVID-19) in Ghana. Focusing on the ubiquity of religions in Ghana, the paper argues that religious experts deploy their ability to invoke the transcendental being to compel adherents to comply with the lockdown rules, as well as follow precautionary measures to fight the virus in Ghana. The paper also maintains that the state has, in the face of the current crisis, passed a law that could be inimical to the future of religious freedom in the country. Methodologically, I draw on critical reflections on electronic and social media reports about religion and humanitarian services in Ghana to argue that religions are bridging the mundane and spiritual gaps to aid the state, while the state is also creatively engaging religions to invest in prayers and also pass laws that are potentially inimical to the future of religious freedom.

Chronicling Country-Specific Response to Covid-19 Pandemic in Africa: From the Perspective of Ghana

Health is closely tied to human development and the overall development of a nation. Indeed, throughout history, improved public health has been a major determinant of development. Within this context, the consequences of infectious diseases including the novel Coronavirus are not in doubt. Particularly, the COVID-19 pandemic has destabilized the much-talked about world civilization with many nations still under various infection control measures. Globally, the pandemic has resulted in several losses in the areas of jobs, income, government revenues, foreign direct investments, among others. Beyond the incalculable economic losses, the pandemic constitutes a great threat to physical, social and mental health. Since the outbreak of the pandemic, several studies have been conducted on the experiences of different countries to help deepen global knowledge on the pandemic. This review paper chronicles the Ghanaian experience of COVID-19 and attempts to probe whether the country’s overall response to the disease is worthy of emulation by other nations. Part one introduces the paper by looking at the intersection of health and development, a brief history of COVID-19, challenges associated with the pandemic and measures some countries have employed to rebuild their economies. Part two describes the key issues underpinning the spread of the virus in Ghana whilst part three considers the efforts key stakeholders employed to contain and manage the spread of the virus. Part four focuses on the challenges Ghana has faced in the management of the pandemic whereas the concluding part presents the core issues which should engage the attention of stakeholders. Keywords: COVID-19 pandemic, misinformation, social risk amplification and attenuation theory, health services, development DOI: 10.7176/NMMC/97-03 Publication date: August 31 st 2021

Assessment of Korean rice lines for their reaction to rice yellow mottle virus in Ghana

Rice yellow mottle virus (RYMV) is the most damaging viral disease of rice in Africa and can cause yield losses of up to 100%. The objective of this study was to characterize newly introduced rice lines from Korea into Ghana for their reaction to RYMV infection. One hundred and seventy-two rice lines from Korea were screened for their level of resistance RYMV in a screen house at Fumesua, Ghana. Four checks consisting of two highly resistant lines (Tog7291 and Gigante with rymv1-2 (resistant gene1-allele2) and rymv2 (resistant gene2) respectively), a moderately resistant line (CRI-Amankwatia) and a susceptible cultivar Jasmine 85 were used. The experiment was carried out in a 4 x 44 lattice design with four replicates. Screening for RYMV resistance was conducted by visual symptom scoring and virus-assessment through serology using enzyme linked immunosorbent assay (ELISA) test. Disease incidence and severity were assessed from 2 to 42 dpi. Data for disease severity and incidence were transformed (Log x+1) for ANOVA.Five lines (8261112, 8261119, 8261133, 8261588, and 8261634) were identified to be highly resistant to the disease just like Tog7291 and Gigante. The study also revealed 24 lines that were resistant but not grouping with Tog7291 and Gigante, whereas 100 moderately resistant lines clustered with the moderately resistance check CRI-Amankwatia in a distinct group. Forty-three (43) susceptible lines were identified with the susceptible check Jasmine 85 falling in this group. No highly susceptible line was identified. The newly idenfied resistant genotypes can be used by breeders to develop RYMV resistant varieties.

Lived Experiences of Adolescent Living with human immunodeficiency virus in Ghana: A Phenomenology Study.

Aim and objectivesTo explore the meaning of living with HIV positive in Ghanaian adolescents.DesignHermeneutic phenomenological approach developed by Van Manen methodology (1990).MethodA purposive sampling of 12 adolescents living with HIV was recruited. Data were collected between September 2019–January 2020 using semi‐structured interviews and analysed using thematic analysis.ResultsTwo main themes emerged: Stigmatization and HIV disclosure and Living with a heavy burden. Seven subthemes were also found. Adolescents living with HIV in Ghana face discrimination, rejection and go through psychological distress such as suicidal thoughts, fear of death and hopelessness.ConclusionThe problems faced by adolescents living with HIV are a result of the inherent beliefs of the Ghanaian society about HIV. Nurses working with ADLHIV should concentrate on identifying challenges and provide support and care, in addition to their treatment.

Fc-Based Recombinant Henipavirus Vaccines Elicit Broad Neutralizing Antibody Responses in Mice.

The genus Henipavirus (HNVs) includes two fatal viruses, namely Nipah virus (NiV) and Hendra virus (HeV). Since 1994, NiV and HeV have been endemic to the Asia–Pacific region and responsible for more than 600 cases of infections. Two emerging HNVs, Ghana virus (GhV) and Mojiang virus (MojV), are speculated to be associated with unrecognized human diseases in Africa and China, respectively. Despite many efforts to develop vaccines against henipaviral diseases, there is presently no licensed human vaccine. As HNVs are highly pathogenic and diverse, it is necessary to develop universal vaccines to prevent future outbreaks. The attachment enveloped glycoprotein (G protein) of HNVs mediates HNV attachment to the host cell’s surface receptors. G proteins have been used as a protective antigen in many vaccine candidates for HNVs. We performed quantitative studies on the antibody responses elicited by the G proteins of NiV, HeV, GhV, and MojV. We found that the G proteins of NiV and HeV elicited only a limited cross-reactive antibody response. Further, there was no cross-protection between MojV, GhV, and highly pathogenic HNVs. We then constructed a bivalent vaccine where the G proteins of NiV and HeV were fused with the human IgG1 Fc domain. The immunogenicity of the bivalent vaccine was compared with that of monovalent vaccines. Our results revealed that the Fc-based bivalent vaccine elicited a potent antibody response against both NiV and HeV. We also constructed a tetravalent Fc heterodimer fusion protein that contains the G protein domains of four HNVs. Immunization with the tetravalent vaccine elicited broad antibody responses against NiV, HeV, GhV, and MojV in mice, indicating compatibility among the four antigens in the Fc-fusion protein. These data suggest that our novel bivalent and tetravalent Fc-fusion proteins may be efficient candidates to prevent HNV infection.