Medical virtual simulation teaching environment is an advanced and complex combination of virtual simulation technology and medical technology training. As an advanced information technology, it has been attracting the attention of medical education researchers. Based on the characteristics of medical laboratory technology education, the virtual simulation experimental teaching center was established and a framework of the virtual simulation training environment was designed in the Department of Laboratory Medicine, Southern Medical University. Virtual simulation softwares and tool softwares were put into use widely in our experimental teaching.As it turns out, this simulated training environment has many merits such as risk–free safety, great efficiency, genuine learning experience and wide application potential, which partly overcomes the restriction of the teaching process brought by the condition of the practical operation and improves the teaching effect significantly. (Chin J Lab Med, 2015, 38: 716–718) Key words: Medical laboratory science; Teaching methods; Computer–assisted instruction; Laboratories, hospital