Traditionally, virtual inertia is used as the control strategy for wind turbines when participating in frequency regulation. However, it has inherent defects in measurement error amplification due to the frequency differential. Also, when control delay of wind turbines is taken into consideration, virtual inertia is essentially a fast power response, just the same as droop control. Hence, considering the adaptability of droop control, it is valuable to explore a novel droop control method without virtual inertia, as the frequency response model of wind turbines. For this reason, a multi-segment droop control strategy is proposed to provide a reliable frequency regulation model for wind turbines that can be broadly applied to other inverter-based power sources. Firstly, based on the extended system frequency response model, a differential equation including piecewise time-varying coefficients is established, and the analytical expression of the frequency response is obtained using the impulse function balancing principle and the integration by parts algorithm. Subsequently, based on analytical expression, the performance of the presented strategy is theoretically analyzed via comparison with virtual inertia control, and the conclusion that the presented multi-segment droop control has better frequency regulation performance can be got. Furthermore, to achieve optimal frequency regulation performance, a Lagrangian function is established to determine the optimal parameters of the proposed control strategy. Finally, the performance of the proposed strategy was verified in a two-area system model constructed on MATLAB/Simulink. Results show that the proposed multi-segment droop control has better frequency regulation performance.