The study aimed to isolate and identify of the types of bacteria which cause gingivitis. Gram positive bacteria were isolated by 89% more than gram-negative bacteria, 60 isolates were Streptococcus viridans and 5 isolates were Escherichia coli. The biochemical study showed a significant increase in concentration of total cholesterol (P 14≤"> 0.05) in the gingivitis and diabetes groups, as well as in very low density lipoproteins showed a significant increase in gingivitis and diabetes group (P 14≤"> 0.033), High-density lipoproteins showed a significant decrease in the group of gingivitis and diabetes compared to the control group at a significant level (P 14≤"> 0.018). In the immunological parameters, the results showed a significant decrease in the concentration of IL-10 in the group gingivitis and diabetes (P 14≤"> 0.05). The concentration of TNF-α increased in gingivitis and diabetes at a significant level (P 14≤"> 0.05).