You have accessJournal of UrologyInfections/Inflammation of the Genitourinary Tract: Prostate & Genitalia1 Apr 20111423 PREVALENCE OF HUMAN PAPILLOMAVIRUS INFECTION OF THE INNER FORESKIN IN MEN WITHOUT CLINICAL SYMPTOMS Michael Ladurner Rennau, Alexander Buttazoni, Renate Pichler, Barbara Schlenk, Gerald Klinglmair, Elisabeth Richter, Helga Fritsch, Bettina Zelger, and Josef Oswald Michael Ladurner RennauMichael Ladurner Rennau Innsbruck, Austria More articles by this author , Alexander ButtazoniAlexander Buttazoni Innsbruck, Austria More articles by this author , Renate PichlerRenate Pichler Innsbruck, Austria More articles by this author , Barbara SchlenkBarbara Schlenk Innsbruck, Austria More articles by this author , Gerald KlinglmairGerald Klinglmair Innsbruck, Austria More articles by this author , Elisabeth RichterElisabeth Richter Innsbruck, Austria More articles by this author , Helga FritschHelga Fritsch Innsbruck, Austria More articles by this author , Bettina ZelgerBettina Zelger Innsbruck, Austria More articles by this author , and Josef OswaldJosef Oswald Innsbruck, Austria More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2011.02.1335AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES It has been well established that human papillomaviruses (HPVs) are the main risk factor for cervical carcinoma in 70 out of 100 cases. We investigated in a prospective study the epidemiology of HPV and its colonization of the male genital tract and in particular the inner layer of the foreskin. METHODS We examined anonymized foreskins of unselected 133 males aged between 7 months and 82 years without clinical HPV associated warts, who underwent radical circumcision. Indication for circumcision was phimosis. The presence of viral (HPV) nucleic acid (DNA) sequences in tissues was demonstrated via in-situ hybridization (ISH) by using labelled DNA probes. DNA extraction of 40 foreskin tissue sections was done to explore viral nucleic acid of HPV by Real-Time RT-PCR. PCR primers were used to detect HPV-DNA of low risk types (6, 11) as well as of high risk types (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 66). These findings were correlated with in-situ hybridization data of the same tissue samples. RESULTS We found a prevalence of low-risk HPV genotypes in foreskins of 18.8%, whereas the prevalence of high-risk HPV genotypes was 9.77%. Gene expression of both high- and low-risk HPV genotypes of the same tissue samples correlated significantly with in-situ hybridisation data. CONCLUSIONS Our study revealed the occurrence of subclinical genital low and high risk HPV infections in boys and men which could be a reservoir for HPV-associated diseases. Since it is proven that viral transfer results from sexual contact it is advisable to vaccinate not only girls but also boys before adolescence. © 2011 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 185Issue 4SApril 2011Page: e570 Advertisement Copyright & Permissions© 2011 by American Urological Association Education and Research, Inc.MetricsAuthor Information Michael Ladurner Rennau Innsbruck, Austria More articles by this author Alexander Buttazoni Innsbruck, Austria More articles by this author Renate Pichler Innsbruck, Austria More articles by this author Barbara Schlenk Innsbruck, Austria More articles by this author Gerald Klinglmair Innsbruck, Austria More articles by this author Elisabeth Richter Innsbruck, Austria More articles by this author Helga Fritsch Innsbruck, Austria More articles by this author Bettina Zelger Innsbruck, Austria More articles by this author Josef Oswald Innsbruck, Austria More articles by this author Expand All Advertisement Advertisement Loading ...