Viola Albida Research Articles

A complete chloroplast genome sequence of Viola albida Palibin 1899 (Violaceae), a member of VIOLA ALBIDA complex

The VIOLA ALBIDA complex is a complicated group with taxonomic problems having continuous leaf variations and composed of taxa related to the following names: Viola albida, V. albida var. takahashii, and V. chaerophylloides. As a first step to understanding the genomic nature of this complex, this study identified the whole chloroplast genome of V. albida. The genome is 157,692 bp in length (36.3% of GC content) and contains four subregions: a large single copy region of 86,220 bp, a small single copy region of 17,248 bp, and a pair of inverted regions of 27,112 bp each. An annotation of the gene identifies 111 unique genes, including 77 protein-coding genes, four rRNA genes, and 30 tRNA genes. The phylogenetic analysis of this genome with selected cp genomes from Viola identifies the close relationship between V. albida and V. ulleungdoensis. It is noteworthy that V. chaerophylloides, traditionally recognized as a member of the VIOLA ALBIDA complex, is genetically distant from V. albida and forms a sister group of all other members of the subsection Patellares. Our genome report is expected to serve as a basis for understanding the identity of the VIOLA ALBIDA complex.

Speciation of <i>Viola albida</i> var. <i>takahashii</i> based on both artificial and natural hybridization between <i>V. albida</i> var. <i>albida</i> and <i>V. albida</i> var. <i>chaerophylloides</i>

The study aims are to examine the characteristics of artificial and natural hybrids between <i>Viola albida</i> var. <i>albida</i> (= <i>albida</i>, from below) and <i>V. albida</i> var. <i>chaerophylloides</i> (= <i>chaerophylloides</i>, from below), and to confirm if hybrids could be fertile and make populations in their native habitats. The 1st filial (= F<sub>1</sub>, from below) leaf shape produced by artificial crossing between <i>albida</i> and <i>chaerophylloides</i> was the same as that of <i>V. albida</i> var. <i>takahashii</i> (= <i>takahashii</i>, from below), and F<sub>1</sub> bore also both chasmogamous and cleistogamous flowers. F<sub>1</sub> seed number was 9.6 per cleistogamous pods, which was remarkably less than the average of 38.2 for <i>albida</i> and <i>chaerophylloides</i>, but the germination rate was all similar. The leaf type of self-crossed 2nd offsprings (= F<sub>2</sub>, from below) showed all leaf types found in the <i>Viola albida</i> complex, but the ratio of <i>chaerophylloides</i> leaf type was relatively low. Individuals whose F<sub>2</sub> leaf type was restored to <i>albida</i> produced an average of 31.4 seeds per capsule, meaning that fertility was restored. On the other hand, individuals of F<sub>2</sub> <i>takahashii</i> leaf type come to fruition a low average of 10.4 seeds per capsule, which is similar to that of <i>takahashii</i>. The results of crossbreeding experiment, where is their native habitats, were similar to that of laboratory. Both <i>albida</i> and <i>chaerophylloids</i> in Mt. Bulmyeong distribute extensively, but <i>takahashii</i> make a small population only in places where <i>albida</i> and <i>chaerophylloides</i> grow together. Summarizing the above results is suggesting that the speciation of <i>takahashii</i> was done by hybrid between <i>albida</i> and <i>chaerophylloides</i>, and these have been maintained with relatively small population by cleistogamous capsules.

A correlation between leaf shape and its related key genes in Viola albida complex

Simple to compound leaves occur in the Viola albida complex, which comprises the simple, finely serrate leaves of V. albida Palib., the deeply lobed leaves of V. albida var. takahashii (Nakai) Nakai, and the compound leaves of Viola chaerophylloides (Regel) W. Becker. To identify a correlation between the different leaf forms and the expression of several key genes with roles in leaf morphogenesis, the distinct leaf forms occurring within these species were generated by tissue culture of the V. chaerophylloides petiole, for comparison with wild-type leaves. Compound leaves were generally formed from a petiole explant taken close to the leaf blade, whereas simple leaves resulted from petiole explants taken close to the petiole base. KNOTTED-1 (VaKN1), SHOOTMERISTEMLESS (VaSTM), CUP-SHAPED COTYLEDON-2 (VaCUC2), and ASYMMETRIC LEAVES 1 (VaAS1), which are known to play key roles during compound leaf patterning and morphogenesis, were isolated and multiple sequence alignment revealed that there was no sequence variation at the amino acid level within each gene and between the three varieties. Phylogenetic analysis confirmed that the isolated genes were homologous to KN, STM, CUC2, and AS1. The expression of VaKN1, VaSTM, and VaCUC2 was significantly elevated in the in vitro-cultured deeply lobed and compound leaves, as well as in V. chaerophylloides and V. albida var. takahashii plants, but was very low in the in vitro-cultured simple leaves and V. albida plants. These findings demonstrated that elevated transcripts of VaKN1, VaSTM, and VaCUC2 lead to the development of compound and deeply lobed leaves in the V. albida complex.

A comparative study of early leaf development in the Viola albida complex

Early leaves within the Viola albida complex were investigated by scanning electron microscopy in order to determine the morphological segments during morphogenesis. The early leaf development of V. albida var. albida could be morphologically divided into the eight stages in the following order: I, the initiation of shoot germination; II, the conical growth directionally of the leaf; III, the adaxial and abaxial formation of the leaf; IV, the initiation of the stipule; V, the formation of a transitional zone between the leaf blade and petiole; VI, the expansion of the upper part of the leaf blade; VII, the formation of almost all parts of the early leaf; VIII, the early simple leaf. Viola albida var. takahashii differs from V. albida var. albida by additional stages, i.e., V-1, the initiation of the first lateral lobe at the both lateral parts of the leaf after the stage V and an early lobed leaf. Viola albida var. chaerophylloides is also distinguished from two taxa by two developmental features, V-2, the initiation of a second lateral lobe below of the first lateral lobe, and an early palmately compound leaf. These findings suggest that the Viola albida complex would be in the process of peramorphosis, showing developmental changes in a chain of events, leading to a different leaf shape. These data would also be useful for isolating genes that give rise to different leaf morphogenesis outcomes among the taxa in the Viola albida complex.

낙남정맥에 분포하는 관속식물상 - 길마재, 무량산, 여항산, 무학산, 천주산, 신어산을 중심으로 -

This study was carried out to investigated the flora of Naknam-Jeongmaek, Korea. The survey sites were Gilmajae, Mt. Muryang, Mt. Yeohang, Mt. Muhak, Mt. Cheonju and Mt. Sineo. The flora were summarized, numbering 666 taxa including 112 families, 357 genera, 574 species, 4 subspecies, 73 varieties and 15 forms. The threatened species was Aconitum austrokoreense. The rare plants were 12 taxa including Asarum maculatum, Wikstroemia ganpi, Viola albida, Tricyrtis macropoda and so forth. The Korean endemic plants were 19 taxa including Salix koriyanagi, Thalictrum actaefolium var. brevistylum, Weigela subsessilis, Hosta minor and so forth. The specific plants by floristic region were 81 taxa including 38 taxa of grade I, 19 taxa of grade II, 16 taxa of grade III, 7 taxa of grade IV and 1 taxa of grade V. The naturalized plants were 32 taxa including Rumex crispus, Physalis angulata, Erechtites hieracifolia, Taraxacum officinale and so forth. The invasive alien plants were Ambrosia artemisiifolia and Aster pilosus.

충남 오서산과 봉수산의 식물상 및 관리방안

The vascular plants in the Ohseosan and the Bongsusan, Chungnam were listed 439 taxa (9.0% of all 4,881 taxa of vascular plants); 95 families, 268 genera, 339 species, 5 subspecies, 75 varieties and 20 forms. Furthermore, the Ohseosan were listed 339 taxa and the Bongsusan were listed 306 taxa. So, Hemicryptophytes (H) were 107 taxa (24.4%), Therophytes (Th), Geophytes (G) same as were 82 taxa (18.7%) showed high proportional ratio in life form. Based on the list of rare plants, 4 taxa; Aristolochia contorta, Viola albida, Scutellaria insignis (LC) and Scrophularia koraiensis (DD) and endemic plants, 8 taxa; Aconitum pseudolaeve, Ajuga spectabilis, etc. Based on the list of specific plants, 35 taxa; Wisteria floribunda for. floribunda in class IV, 5 taxa (Scutellaria insignis, Scrophularia koraiensis, etc.) in class III, 5 taxa (Acer triflorum, Cymopterus melanotilingia, etc.) in class II, 24 taxa (Pyrus ussuriensis var. ussuriensis, Campanula punctata, etc.) in class I. Based on the list of naturalized plants, 9 families, 20 genera, 24 taxa (Persicaria orientalis, Carduus crispus, etc.) and ecosystem disturbing plants were Rumex acetocella, Ambrosia artemisiifolia. Naturalization rate was 5.5% of all 439 taxa of vascular plants and urbanization index was 7.5% of all 321 taxa of naturalized plants. In particular, for rare plants, in and ex-situ conservation of genetic resources must surely be done, by preserving present natural habitats, discovering additional natural habitats and securing seeds. Moreover, ecosystem disturbing plants require long-term monitoring and consistent management, since not only do they disturb the ecosystem in competition with Korean native species, but damage humans, too.

가야산국립공원의 식물상과 보전방안

This study is to offer the raw data for conservation and restoration of national park by surveying and analysing the vascular plants in the Gayasan national park. The flora were summarized as 102 families, 328 genera, 469 species, 4 subspecies, 59 varieties and 9 forms. The endangered plants designated by Ministry of Environment were 2 taxa; Pedicularis hallaisanensis Hurus. and Gymnadenia conopsea for. albiflora Y.N.Lee. The rare plants designated by Korea Forest Service were 13 taxa; Crypsinus hastatus (Thunb.) Copel., Ligusticum tachiroei (Franch. & Sav.) M. Hiroe & Constance, Primula modesta var. fauriae (Franch.) Takeda, Lilium distichum Nakai ex Kamibay and so forth. The korean endemic plants were 15 taxa; Betula ermanii Cham., Carpinus laxiflora (Siebold & Zucc.) Blume, Stewartia pseudocamellia Maxim., Galium koreanum (Nakai) Nakai, Heloniopsis koreana Fuse, N.S. Lee & M.N. Tamura and so forth. The specific plants by floristic region were 66 taxa; Betula chinensis Maxim., Spiraea fritschiana Schneid., Vaccinium hirtum var. koreanum (Nakai) Kitam., Cimicifuga heracleifolia Kom., Adoxa moschatellina L. and so forth. The plants with approval for delivering oversea were 22 taxa; Salix hallaisanensis H.L<TEX>$\acute{e}$</TEX>v., Sanguisorba argutidens Nakai, Viola albida Palib., Weigela subsessilis (Nakai) L.H.Bailey and so forth. The naturalized plants were 30 taxa; Chenopodium album L., Lepidium apetalum Willd., Trifolium pratense L., Bidens frondosa L., Helianthus tuberosus L. and so forth.

장안산과 팔공산 구간(전북 장수)의 식물상 및 보전관리방안

The vascular plants in the trail from Jangansan to Palgongsan were listed 389 taxa (8.0% of all 4,881 taxa of Korean vascular plants); 84 families, 226 genera, 325 species, 1 subspecies, 55 varieties and 8 forms. Furthermore, hemicryptophytes (H) were 110 taxa (28.3%), geophytes (G) were 77 taxa (19.8%), megaphanerophytes (MM) were 48 taxa (12.3%), nanophanerophytes (N) were 47 taxa (12.1%) showed high proportional ratio in life form. So, resource plants of 237 taxa of edible (60.9%), 278 taxa of medicinal (71.5%), 229 taxa of ornamental (58.9%) and 178 taxa of the others (45.8%). Based on the list of rare plants, 7 taxa (1.8% of all 389 taxa of flora and 1.2% of all 571 taxa of rare plants); Paeonia japonica (VU), Viola albida, Viola diamantica, Lilium distichum, Disporum ovale, Tricyrtis dilatata, Iris ensata var. spontanea (LC), etc. Based on the list of endemic plants, 7 taxa (1.8% of all 389 taxa of flora and 2.1% of all 328 taxa of endemic plants); Salix koriyanagi, Aconitum pseudolaeve var. erectum, Thalictrum actaefolium var. brevistylum, Philadelphus schrenckii, Asperula lasiantha, Weigela subsessilis, Carex okamotoi, etc. Based on the list of specific plants, 46 taxa (11.8% of all 389 taxa of flora and 4.3% of all 1,071 taxa of specific plants); 2 taxa (Wisteria floribunda for. floribunda, Cardamine yezoensis, etc.) in class IV, 6 taxa (Acer palmatum, Ulmus davidiana, etc.) in class III, 14 taxa (Ligularia fischeri, Cymopterus melanotilingia, etc.) in class II, 24 taxa (Ilex macropoda, Fraxinus mandshurica, etc.) in class I. Based on the list of naturalized plants, 6 families, 16 genera, 18 taxa (Phytolacca americana, Robinia pseudoacacia, Oenothera odorata, Erechtites hieracifolia, Festuca arundinacea, etc.) and invasive alien plants were 3 taxa; Rumex acetocella, Ambrosia artemisiifolia, Aster pilosus. Naturalization rate (NR) was 4.6% of all 389 taxa of vascular plants and urbanization index (UI) was 5.6% of all 321 taxa of naturalized plants.

경상남도 양산시 주요지역의 관속식물상과 관리방안 - 천태산, 낙동강습지, 법기수원지, 양산천, 천성산을 중심으로 -

The purpose of this study is to offer the raw data for conserving the biodiversity of urban ecosystem by objective surveying and analysing the vascular plants distributed in major sites of Yangsan-si, Gyeongsangnam-do. The results are as follows. The numbers of vascular plants were summarized as 522 taxa including 110 families, 314 genera, 459 species, 4 subspecies, 46 varieties and 13 forms. The rare plants were 5 taxa including Aristolochia contorta, Drosera rotundifolia, Potentilla discolor, Viola albida and Hydrocharis dubia. The endemic plants were 13 taxa including Salix koriyanagi, Carpinus laxiflora, Stewartia pseudocamellia, Weigela subsessilis and so forth. The specific plants by floristic region were 30 taxa including Caltha palustris, Caryopteris incana, Ilex macropoda, Monochoria korsakowii and so forth. The naturalized plants were 44 taxa including Phytolacca americana, Lepidium virginicum, Solanum americanum, Aster subulatus var. sandwicensis, Panicum dichotomiflorum and so forth. The invasive alien plants were 5 taxa including Sicyos angulatus, Ambrosia artemisiifolia, Ambrosia trifida, Aster pilosus and Paspalum distichum.

북한산국립공원 주요지역의 관속식물상 -북한산 둘레길, 진관내동 습지, 북한동 철거지를 대상으로-

The vascular plants in Bukhansan Dulegil, Jingwan-dong Wetland and Bukhan-dong Cheolgeoji were listed 432 taxa (8.9% of all 4,881 taxa of vascular plants); 95 families, 274 genera, 345 species, 2 subspecies, 69 varieties and 16 forms. Divided into woody plants were 145 taxa (33.6%) and herbaceous plants were 287 taxa (66.4%). The Bukhansan Dulegil were listed (Myeongsang-gil 159 taxa, Huingureum-gil 227 taxa, Wooyiryeong-gil 216 taxa, Banghakdong-gil 139 taxa, Boru-gil 199 taxa), Jingwan-dong Wetland were listed 147 taxa and Bukhan-dong Cheolgeoji were listed 129 taxa. Based on the list of rare plants by Korea Forest Service and Korea National Arboretum were Viola albida Palibin (Violaceae), Iris minutiaurea Makino (Iridaceae) and Korea National Arboretum were endemic plants, Philadelphus schrenkii Rupr. var. schrenkii (Saxifragaceae), Weigela subsessilis L.H. Bailey (Caprifoliaceae). Based on the list of floristic regional indicator plants by Korean Ministry of Environment were total 22 taxa; Wisteria floribunda DC. for. floribunda in class IV, 3 taxa (Betula davurica Pall., Mukdenia rossii Koidz., Glechoma grandis Kuprian., etc.) in class III, 5 taxa (Acer triflorum Kom., Viola orientalis W. Becker, Heloniopsis koreana Fuse & Al., etc.) in class II and 13 taxa (Camptosorus sibiricus Rupr., Clematis patens C. Morren & Decne., Cirsium pendulum Fisch. ex DC., etc.) in class I. Based on the list of naturalized plants, 16 families, 35 genera, 37 species, 3 varieties, 1 form, total 41 taxa (Rumex crispus L., Abutilon theophrasti Medicus, Panicum dichotomiflorum Michx. etc.), naturalization rate was 9.5% of all 432 taxa of vascular plants and urbanization index was 13.3% of all 309 taxa of naturalized plants. Ecosystem disturbing wild plants were 5 taxa; Rumex acetocella L., Ambrosia artemisiifolia L., Ambrosia trifida L. var. trifida, Eupatorium rugosum Houtt., Aster pilosus Willd.

Taxonomic study of Viola albida complex based on RAPD data

A taxonomic study of Viola albida complex, containing the representative individuals of three taxa, V. albida var. albida, V. albida var. chaerophylloides, and V. albida var. takahashii, was done based on RAPD data. The amplified loci were 476 in total; obtained with 68 universal primers on seven OTUs. Nei's genetic dissimilarity appeared relatively low within individuals of V. albida var. albida and V. albida var. chaerophylloides (0.118-0.171 and 0.051 respectively), however, it was higher in individuals of V. albida var. takahashii (0.348). On the other hand, there is no specific trend in terms of genetic dissimilartiy among taxa, such as between individuals of V. albida var. albida and V. albida var. takahashii, between those of V. albida var. albida and V. albida var. chaerophylloides, and between those of V. albida var. albida and V. albida var. takahashii. The similarity of OTUs studied is high in clustering analysis, so that this result is compatible with the establishment of this complex. All OTUs are clustered within two groups. The individuals of V. albida var. takahashii, however, are clustered both to the group of V. albida var. albida and to the group of V. albida var. chaerophylloides, meaning that the genetic difference is high which would be commensurate with their morphological variations.

Vegetation and Flora in the Cheonbansan (Mt.), Jinan

The vascular plants in the Cheonbansan were listed 241 taxa; 72 families, 157 genera, 307 species, 27 varieties and 7 forms. Divided into 241 taxa; woody plants were 95 taxa (39.4%) and herbaceous plants were 146 taxa (60.6%). Based on the rare plants (IUCN) by the Korea Forest Service and Korea National Arboretum, 2 taxa (Viola albida, etc.). Based on the list of Korean endemic plants, 8 taxa (Salix koriyanagi, Lespedeza maximowiczii for. tomentella, Indigofera koreana, Weigela subsessilis, Carex okamotoi, Lilium amabile, etc.). Specific plant species by floral region were total 15 taxa (6.2% of all 241 taxa of vascular plants); Lonicera subhispida in class, Orostachys sikokianus, Ostericum melanotilingia in class, 9 taxa (Betula schmidtii, Quercus variabilis, Clematis patens, Spiraea blumei, Ilex macropoda, Vaccinium oldhami, Lonicera praeflorens, Microstegium japonicum, Hosta capitata) in class. The naturalized plants in the surveyed sites were 8 families, 13 genera, 16 taxa (Rumex acetocella, Silene armeria, Lepidium apetalum, Trifolium pratense, Oenothera odorata, Cuscuta pentagona, Xanthium strumarium, Eragrostis curvula, etc.) and naturalization rate was 6.6% of all 241 taxa of vascular plants. The vegetation of results in the Cheonbansan, a total of 4 plant communities; Pinus densiflora community, Quercus variabilis community, Quercus serrata community, Quercus mongolica community. The occupied in low-slope and valley of Quercus serrata community, mountain side of Pinus densiflora community, Quercus variabilis community, top and ridge of Quercus mongolica community.

Dispersion of Vascular Plant in Mt. Huiyangsan, Korea

We surveyed that vascular plants can be classified into 90 families and 240 genus, 336 species, 69 variants, 22 forms, 3 subspecies, total 430 taxa. Dicotyledon plant is 80.9%, monocotyledon plant is 9.8%, Pteridophyta is 8.1%, Gymnosermae is 1.2% among the whole plant family. Rare and endangered plants are Crypsinus hastatus, Lilium distichum, Viola albida, Rhododendron micranthum, totalling four species. Endemic plants are Carex okamotoi, Salix koriyanagi for. koriyanagi, Clematis trichotoma, Thalictrum actaefolium var. brevistylum, Galium trachyspermum, Asperula lasiantha, Weigela subsessilis, Adenophora verticillata var. hirsuta, Aster koraiensis, Cirsium chanroenicum and Saussurea seoulensis total 11 taxa. Specialized plants are 20 classification for I class, 7 classifications for the II class, 7 classifications for the III class, 2 classification for the IV class, and 1 classification for the V class, total 84 taxa. Naturalized plants specified in this study are 10 types but Naturalization rate is not high compared to the area of BaekDu-DaeGan. This survey area is focused on the center of BaekDu-DaeGan, and it has been affected by excessive investigations and this area has been preserved as Buddhist temples’ woods. Therefore, we need health care provisions and monitoring for this.

계룡산국립공원의 관속식물상

The vascular plants in the Gyeryongsan National Park were listed 227 taxa; 71 families, 148 genera, 196 species, 1 subspecies, 26 varieties and 4 forms, Among them, trees and herbs were 103 taxa (45.4%), 124 taxa (54.6%), respectively. The rare plants were identified 6 taxa such as Paeonia obovata (Legal protection species), Viola albida, Acanthopanax chiisanensis, Lilium distichum, Lilium callosum and Goodyera schlechtendaliana by the Korea Forest Service. Based on the list of Korean endemic plants, 8 taxa were recorded; Cephalotaxus koreana, Pseudostellaria multiflora, Thalictrum uchiyamai, Indigofera koreana, Acanthopanax chiisanensis, Asperula lasiantha, Weigela subsessilis and Carex okamotoi. Based on the list of approved for delivering overseas of plants, 6 taxa were recorded; Viola albida, Acanthopanax chiisanensis, Asperula lasiantha, Carex okamotoi, Lilium distichum and Lilium callosum. Specific plant species by floral region were total 21 taxa (9.3%); Paeonia obovata in class V, 5 taxa (Betula davurica, Acer palmatum, Asperula lasiantha, Lonicera subhispida, Phaenosperma globosa) in class III, 4 taxa (Viola orientalis, Acanthopanax chiisanensis, Ostericum melanotilingia, Lilium distichum) in class II and 11 taxa (Cephalotaxus koreana, Betula schmidtii, Quercus variabilis, Aphananthe aspera, Ilex macropoda, Meliosma oldhamii, Tilia amurensis, Lilium callosum, Erythronium japonicum, Polygonatum inflatum, Hosta capitata) in class I. It is important that relationship between national and local government will be needed for preservation of natural environment of Gyeryongsan National Park. As well, a long time of monitering and systematical management counterplan will be required.

Taxonomic study on Viola albida var. albida and its related taxa

Taxonomic study on Viola albida var. albida and its related taxa

Interspecific relationships of Korean Viola based on RAPD, ISSR and PCR-RFLP analyses

Molecular taxonomic studies were conducted to evaluate interspecific relationships in Korean Viola 34 taxa including two Japanese populations using RAPD(randornly amplified polymorphic DNA), ISSR(inter simple sequence repeat) and PCR-RFLP(restriction fragment length polymorphism) analysis. Only six and four primers out of 40 arbitrary and 12 ISSR primers were screened for 34 taxa, and were revealed 70 (98.6%) and 28 (96.6%) polymorphic bands, respectively. Fifteen restriction endonucleases produced 80 restriction sites and size variations from the large single copy region of cpDNA, 16 (20%) of which were polymorphic. The separate analyses from the RAPD, ISSR and PCR-RFLP data were incongruent in the relationships among 34 taxa, but combined data was in accordance with previous infrageneric classification system based on morphological characters, especially the subsection and series level. Section Chamaemelanium placed between subsect. Patellares and Vagimtae of section Nomimium was not formed as a distinct group. Viola alb ida complex including three very closely related taxa was recognized independent group within subsect. Patellares in combined data tree. This result strongly suggested that they should be treated to series Pinmtae. RAPD analysis was very useful to clarify the interspecific relationships among the species of Korean Viola than ISSH and PCR-RFLP analyses.