Vigor Of Seeds Research Articles

Sugar Receding in Aril Benefits the Recalcitrant Seeds of Litchi (Litchi chinensis) and Longan (Dimocarpus longan) to Cope with Dry Spells after Maturation

Litchi and longan are tropic/subtropic fruits harboring recalcitrant seeds that are covered with sugar-rich arils as the edible part. A rapid sugar content decline, called sugar receding, in the arils after the fruit maturation largely reduces the fruit quality, however, the mechanism is unclear. Litchi and longan fruits grow and mature in a hot and rainy season with dry spells between rainfalls. Here, we found that the seed maturation was around 2 weeks earlier than the fruit maturation, after which the sugar contents of the arils significantly decreased, while the fruits could stay on the tree for 1–2 months with high seed vigor. A continuously low-level fruit drop after the seed maturation resulted in continuous seed germination and seedling establishment in around 2–3 months. Blocking assimilate supply by storage of detached fruits or on-tree girdling-plus-defoliation for 7 days, the aril sugar contents of the treated fruits significantly decreased, while the sugar levels and vigor of the seeds increased, implying that the arils are sugar sources to maintain or even enhance the seed vigor and suggesting that sugar receding in arils benefits the recalcitrant seeds of litchi and longan to cope with dry spells after maturation.

Germination test of maize (Zea mays L.) seeds in a NaCl solution

R bjective: To evaluate the germination behavior and to define the initial vigor of maize seeds. Methodology: The genetic material consisted of four batches of 20 hybrid seeds each (M-2014, SR-2014, SR-2012, and SR-2011), which were germinated in a NaCl solution (0, 5, 10, and 15 dS m-1) in a germination chamber. A randomized complete block experimental design was used, in which the seed batch and salinity level were the experimental units, with four repetitions each. Seeds with defined radicle and plumule were counted for evaluation. Results: As a result of the comparison of the three batches from the same environment (production site) and different years, the statistical analysis indicated that the longest-lived seeds have a lower germination percentage. Similarly, when batches from different production environments and the same year were compared, M-2014 obtained the highest average germination percentage (92.19%). When the maximum level of salinity (15 dS m-1) was evaluated, an average 41.25% loss in germination was observed. Conclusions: The test efficiently discriminated seed batches, based on speed and uniformity in NaCl-based germination, therefore providing acceptable results for the evaluation of the viability and vigor of a seed batch.

Ultrasound promoting vigor and germination in seeds of Amburana cearensis

As populações de Amburana cearensis (AC) têm diminuído no bioma Caatinga por conta da atividade extrativista. A implantação de campos comerciais é importante, demandando tecnologias sustentáveis, como o ultrassom (US), para melhorar o vigor e a germinação de sementes. Este trabalho teve por objetivo testar o tratamento de sementes de AC com o uso de US, para melhorar o vigor e a qualidade das plântulas. Para isso, sementes de AC foram submetidas à exposição de 0, 1, 2, 3 e 4 min em US, sendo testadas quanto ao vigor e qualidade. Os resultados foram analisados por ANOVA e teste de Tukey. O tratamento de sementes de A. cearensis em armazenamento com 3 min de exposição em US (42 kHz) pode ser interessante para o manejo silvicultural dessa espécie.

Analysis of seedling images to evaluate the physiological potential of soybean seeds

The use of artificial intelligence techniques collaborate in laboratory analysis of seeds. In this sense, the objective of this work was to verify the efficiency of the Ground Eye® equipment in evaluating the vigor of soybean seeds and the agronomic performance of soybean varieties 74I77 RSF IPRO and NT 1478SP. For this, experiments were carried out to analyze the germination of soybean seeds. The variables analyzed by the analyst and Ground Eye® equipment were the following: number of normal, intermediate, weak and abnormal seedlings. The tetrazolium, vigor and seedling emergence test under field conditions for soybean cultivars were also performed. For seedling emergence in the field, tetrazolium test and vigor there was no statistical difference between soybean cultivars. The Ground Eye Series L equipment showed a lower number of normal seedlings, number of intermediate seedlings, number of weak seedlings and a higher number of vigorous seedlings when compared to the analyzes carried out manually. Based on the results, the cultivar NT 1478SP showed better performance and may be promising in the field. The Ground Eye L Series equipment is an efficient tool for checking the vigor of soybean seedlings, helping the analyst to perform the tests more quickly.

Effect of hydrogen sulfide (H2S) on the growth and development of tobacco seedlings in absence of stress

BackgroundHydrogen sulfide (H2S) is a novel signaling molecule involved in the growth and development of plants and their response to stress. However, the involvement of H2S in promoting the growth and development of tobacco plants is still unclear.ResultsIn this study, we explored the effect of pre-soaking or irrigating the roots of tobacco plants with 0.0, 2.0, 4.0, 6.0, and 8.0 mM of sodium hydrosulfide (NaHS) on endogenous H2S production, antioxidant enzymatic and cysteine desulfhydrase activities, seed germination, agronomic traits, photosynthetic pigments contents, and root vigor. The results revealed that exogenous NaHS treatment could significantly promote endogenous H2S production by inducing gene expression of D/L-CD and the activities of D/L-CD enzymes. Additionally, a significant increase in the agronomic traits and the contents of photosynthetic pigments, and no significant difference in carotenoid content among tobacco plants treated with 0.0 to 8.0 mM of NaHS was observed. Additionally, a significant increase in the germination speed, dry weight, and vigor of tobacco seeds, whereas no significant effect on the percentage of seed germination was observed on NaHS treatment. Furthermore, NaHS treatment could significantly increase the activity of superoxide dismutase (SOD) and peroxidase (POD) enzymes, which reduces damage due to oxidative stress by maintaining reactive oxygen species homeostasis.ConclusionsThese results would aid in enhancing our understanding of the involvement of H2S, a novel signaling molecule to promote the growth and development of tobacco plants.

Seed vigour testing of Acacia mangium Willd. as a result of mutation

Plant breeding techniques are applied to increase the genetic variation of a plant. Induction of plant mutation using gamma irradiation is one of the methods that can be applied to improve the genetic diversity of plants. This study aims to analyze the vigour of mangium seeds treated by mutation. Mutation treatment was administered to M0 of mangium seed. This study used M2 mangium seeds and control mangium seeds (without mutation treatment). Several assays, such as growth, stress, and biochemical tests, were conducted to determine the mangium seed’s vigour. The growth test showed that M2 mangium seeds had higher germination values, germination height, dry weight, and vigour index than control mangium seeds. The stress test consisting of the Hiltner test, accelerated ageing test, and saturation test of the germination value of M2 mangium seeds wass higher than control mangium seeds. In the biochemical test, the electrical conductivity value of control mangium seeds was more significant than M2 mangium seeds. In general, M2 mangium seeds had higher vigour than control mangium seeds.

Determination of efficiency degrees on vigor and antioxidant content of after ripening treatment in eggplant seeds

The research was carried out over a period of two years to study the effects of ripening treatments at different temperatures on the viability, vigour and antioxidant composition of seeds of eggplant varieties ‘Kemer 27’, ‘Aydın Siyahı’ and ‘Manisa Kır Çizgili’ harvested at different periods. In the study, the controlled deterioration tests were kept at two different moisture content and the measurements of the electrical conductivity test were carried out in four different times. Antioxidant analyses were conducted individually for each maturity period. In both years, there were statistically significant differences in all maturation treatments of the cultivars ‘Aydn Siyah’, ‘Kemer 27’, and ‘Manisa Kır Çizgili’ as determined by the controlled deterioration test with 18% and 20% seed moisture content, electrical conductivity, and antioxidant tests for all ripeness seed groups (p<0.05). In the germination test of the control group seeds at 30 °C, the best results were between 58% and 99%, while the highest germination rates at the end of the controlled deterioration test ranged between 97% and 100%. After analysing the test results, it was found that the seed groups subjected to the 20/35 °C and 35 °C ripening processes performed better than the control group. Furthermore, it was found that the seeds obtained from the fruits harvested on the 75th day after anthesis (DAA) had higher viability than the other groups.

Non-destructive detection of single corn seed vigor based on visible/near-infrared spatially resolved spectroscopy combined with chemometrics

Seed vigor is an essential quality evaluation index for seed selection. However, accurately detecting the vigor of a single corn seed is challenging. In this study, we constructed a single-fiber spatially resolved detection device using visible/near-infrared spectroscopy to investigate the patterns and correlations between spatially resolved spectroscopy (SRS) at 500–1000 nm and seed vigor. The device collected spectral data at a light source–detector distance of 5–6.6 mm on the embryo side (S1) and endosperm side (S2) of the corn seeds. The proposed spectral ratio method based on SRS and spectral combination analysis achieved an improvement in the detection accuracy of different corn seed vigor. Modeling by SG-CARS-PLSDA using the ratio method showed further improvement in the prediction ability. The highest accuracy for both S1 and S2 in the Zhengdan 958 variety was 91.67 %, while those of S1 and S2 for the Shaandan 650 variety were 86.67 % and 88.33 %, respectively. In addition, SRS was found to be more advantageous in S2 acquisition, verifying the potential of SRS in the non-destructive testing of seed vigor. This provides a favorable reference for the comprehensive evaluation of other internal quality indices of seeds.

Genome-Wide Association Analysis of Seed Vigor-Related Traits in Wheat

Seed vigor is a crucial indicator comprehensively assessing the quality of seeds, reflecting the growth advantage and production potential of seeds, and has a significant effect on seeds’ stress resistance. Identifying and controlling loci related to wheat seed vigor is essential for accelerating genetic trait gains. Here, we performed a large genome-wide association study (GWAS) to identify several significant quantitative trait loci (QTLs) associated with seed vigor-related traits. A total of 404 wheat samples with diverse genetic backgrounds were used as experimental materials. Twenty-eight loci significantly associated with seed vigor-related traits in wheat were identified, distributed on chromosomes 3A, 4A, 5B, 7A, and 7B. Two potential novel loci controlling wheat seed vigor were discovered, with a total of 80 candidate genes associated with seed vigor located on these loci. Among them, TraesCS4A01G020000.1 encodes a late embryogenesis abundant (LEA) protein gene, and TraesCS5B01G298500.1 encodes a helicase gene, both showing specific expression in seeds and highly correlated with seed vigor. Overall, these findings provide valuable insights for the future application of these genes in wheat breeding.

Effects of panicle position on seed viability and vigor in some selected castor seed accessions

Castor bean is widely cultivated around the world because of the commercial importance for its oil. The fruits of castor are set on a panicle-like structure called raceme. The racemes are classified into the primary, secondary and tertiary racemes as a result of their different time of initiation on the mother plant. The possibility that the raceme initiation points could affect seed viability attributes through its association with time to seed set exists. This study, therefore, determines variation in seed viability and vigor of seeds originating from different racemes positions on the same mother plant. The germination study was conducted in a screen house with 7 castor accessions using sand as a substratum. They were planted in the field under rain-fed conditions in a randomized complete block design with three replications. The castor seeds were planted in 3 rows plot with intra- and inter-row spacing of 1 m. Three seeds from each accession were sown per hole. The racemes were found to be statistically similar in terms of germination ability. However, secondary racemes had the highest mean (64.4%) for the germination percentage and germination rate index (GI) of 5.9. Accession NKAN had the higher germination ability with mean germination percentage of 68.9 and germination rate index (GI) of 6.4. In conclusion, the seed viability and vigor of castor seeds are not significantly affected by raceme positions on the mother plant.

Effect of Rapid Desiccation with Ethanol Immersion on Viability and Vigor of Some Rice Seeds (Oryza sativa L.)

The availability of quality seeds is important in crop cultivation, so quality seeds must be available when farmers need them. Quality seeds must be able to be stored for a long time. Rapid aging through soaking with 95% ethanol within 5 minutes, can predict the shelf life of seeds. Rice seeds that were treated with rapid soaking were Inpari 45 (V1), Sunggal (V2), Memberamo (V3) and Ciherang (V4) varieties. From the results of the analysis of variance, the observation parameters of Germination Power (DB), Maximum Growth Potential (PTM) and Keserempakan Tumbuh (KST) were significantly different (1%). Of the four varieties, after the seeds were soaked in 95% ethanol for 5 minutes DB, PTM and KST decreased very drastically. The order of varieties based on their seed viability and vigor levels, from high to low, are Inpari 45 (V1), Memberamo (V3) Sunggal (V2) and Ciherang (V4). Inpari 45 (V1) has the highest germination rate (DB) of 39%, while Ciherang (V4) has the lowest germination rate (DB) of 15.67%. These results suggest that the Inpari 45 (V1) variety can be stored longer than the other three varieties. Viability and vigor of seeds are not only controlled by environmental factors but also influenced by genetic factors
 
 napid desiccatio, rice varieties, seed viability and vigor

Effect of Zinc Oxide Nanoparticle Based Seed Priming for Enhancing Seed Vigour and Physio-Biochemical Quality of Tomato Seedlings under Salinity Stress

Effect of Zinc Oxide Nanoparticle Based Seed Priming for Enhancing Seed Vigour and Physio-Biochemical Quality of Tomato Seedlings under Salinity Stress

Maternal Effects of Habitats Induce Stronger Salt Tolerance in Early-Stage Offspring of Glycyrrhiza uralensis from Salinized Habitats Compared with Those from Non-Salinized Habitats.

(1) Wild Glycyrrhiza uralensis Fisch (licorice) seeds from different habitats are often mixed for cultivation. However, differences in the responses of seeds from different habitats to salt at the early-stage offspring stage are unclear. (2) Our objective was to evaluate the salt tolerance of G. uralensis germplasms by comparing differences in seed germination and seedling vigor in salinized (abandoned farmland and meadow) and non-salinized (corn farmland edge) soil habitats under different sodium chloride (NaCl) concentrations. (3) The germination rates and germination indexes of seeds from the two salinized habitats with 0-320 mmol·L-1 NaCl were higher and their germination initiation times were earlier. Only seeds from salinized habitats were able to elongate their germs at 240 mmol·L-1 NaCl. Seedlings from salinized habitats had higher fresh weights and relative water contents, while they exhibited lower accumulation of malondialdehyde and less cell electrolyte leakages. Under NaCl treatment, seedlings from the salinized habitats displayed higher superoxide dismutase, catalase, and peroxidase (SOD, CAT, and POD) activities and lower superoxide anion and hydrogen peroxide (O2- and H2O2) contents. Their comprehensive scores showed that the vigor of licorice seeds from salinized habitats was higher. (4) The salt tolerances of different wild G. uralensis seeds were different, and the offspring of licorice from salinized habitats had stronger early-stage salt tolerances.

Codium fragile (Suringar) Hariot as Biostimulant Agent to Alleviate Salt Stress in Durum Wheat: Preliminary Results from Germination Trials.

Soil salinization is a critical environmental problem in arid and semiarid regions of the world. The aim of the present study was to evaluate the effect of an algae-based biostimulant on germination and seedling vigour of durum wheat (Triticum turgidum L. subsp. durum (Desf.) Husn.), under different saline conditions (0, 100, and 200 mM NaCl). The experiment was carried out under controlled-environment conditions. Seeds were sprayed with a solution containing a combination of fungicide and different concentrations of Codium fragile (Suringar) Hariot algae (0%w/v, 10%w/v, 20%w/v, and 30%w/v). All experimental units were placed in a germination cabinet. The effect of the seaweed extract (SWE) on seed germination and seedling performance under salinity stress was evaluated over a period of 8 days. Coleoptile length and biomass were found to be significantly and positively affected by the application of different SWE doses as compared to the control treatment (0% algae). As for germination traits, seeds treated with SWE showed a final germination (from 82% to 88%), under severe saline conditions, significantly higher than that observed in the control treatment (61%). Our findings indicate that the appropriate dose of biostimulant can markedly improve the germination and the seedlings vigour of durum wheat seeds under saline conditions. Additional studies will be needed to understand the mechanism of action of this biostimulant and its effectiveness in longer studies under field conditions.

Seed Germination and Seed Vigour Induction through Foliar Application of Plant Growth Regulators and Nutrients under Drought Stress in Chickpea (Cicer arietinum L.)

Chickpea cultivation in rainfed regions, such as Madhya Pradesh, faces challenges due to water stress, impacting crop yield and quality. The study conducted assesses the impact of foliar spray with plant growth regulators and nutrients on the germination percentage, seedling length, and seed vigour indices of chickpea varieties (JG 36 and JG 14) under water deficit conditions. Cytokinin analogues, known for breaking dormancy, and other growth regulators and nutrients were applied during the flowering stage. The results revealed significant differences in germination percentage, seedling length, seed vigour index I, and seed vigour index II among irrigation levels, varieties, and foliar spray of plant growth regulators and nutrients. Under different irrigation levels, D1 (Irrigation at 30 DAS and at flower initiation) recorded the highest germination percentage (97.24%), seedling length (31.41 cm), seed vigour index I (3054.81), and seed vigour index II (52.77) compared to control D2 (Drought stress at Flowering up to physiological maturity). JG 14 (V1) performed well, achieving the highest germination percentage of 96.99%. Among the treatments, foliar application of Benzyladenine (BA) at 40 ppm (T3) resulted in the highest germination percentage (98.17%) and seed vigour index II (60.33), whereas T8 (BA 40ppm + ZnSO4 1%) exhibited the highest seedling length (31.83 cm) and seed vigour index I (3099.85). This study demonstrates that foliar spray with plant growth regulators and nutrients, particularly Benzyladenine at 40 ppm, significantly improved germination percentage, seedling length, and seed vigour index, offering potential solutions for enhancing chickpea productivity under water deficit conditions.

Siberian Wildrye (Elymus sibiricus) Seed Vigor Estimation for the Prediction of Emergence Performance under Diverse Environmental Conditions

Seed vigor is an important aspect of seed quality. It is critical to predict seed vigor and plant seedling emergence under diverse environmental conditions using the laboratory vigor test. Accordingly, laboratory experiments were conducted to determine the standard germination (SG), early individual counts of radicle emergence (RE), mean germination time (MGT), and vigor index (VI) for 16 commercially available seed lots of Siberian wildrye (Elymus sibiricus), an economically and ecological important grass species. The field emergence (FE) for seed lots was explored using a three-year field trial from 2021 to 2023. Meanwhile, pot experiments were carried out to determine the seedling emergence performance under different environmental conditions, including control, drought, and salinity stress. The correlation and regression analysis were done to investigate the links between laboratory vigor test with emergence performance under both field and pot conditions. The results showed that the SG of 16 seed lots were high, similar, and did not differ significantly between seed lots. However, the seedling emergence performance (emergence percentage, seedling dry weight, and simplified vigor index) differed significantly between seed lots under both field and pot conditions. The SG was not significantly correlated with seedling emergence performance under either the field or pot conditions. The counts of RE at selected timing, MGT, and VI showed a significant relationship with seed vigor as reflected by seedling emergence performance under diverse environmental conditions, and we found that RE at 108 h was highly predictive of seed vigor and seedling emergence. The RE at 108 h and VI were positively related to seed vigor, while MGT was negatively related. The seed lots with low vigor had lower RE at 108 h and VI but longer MGT. Overall, both RE at 108 h, MGT, and VI can be used to estimate seed vigor and predict seedling emergence under different environmental conditions, and we highly recommend RE at 108 h as a quick, precise, and convenient vigor test and early warning sign for seed storage of E. sibiricus. These results will enable seed researchers, plant breeders, farmers, and government program directors to target higher seed vigor more effectively for E. sibiricus and similar grass species.

Growth and transcriptional response of wheat and rice to the tertiary amine BMVE.

Seed vigor is largely a product of sound seed development, maturation processes, genetics, and storage conditions. It is a crucial factor impacting plant growth and crop yield and is negatively affected by unfavorable environmental conditions, which can include drought and heat as well as cold wet conditions. The latter leads to slow germination and increased seedling susceptibility to pathogens. Prior research has shown that a class of plant growth regulators called substituted tertiary amines (STAs) can enhance seed germination, seedling growth, and crop productivity. However, inconsistent benefits have limited STA adoption on a commercial scale. We developed a novel seed treatment protocol to evaluate the efficacy of 2-(N-methyl benzyl aminoethyl)-3-methyl butanoate (BMVE), which has shown promise as a crop seed treatment in field trials. Transcriptomic analysis of rice seedlings 24 h after BMVE treatment was done to identify the molecular basis for the improved seedling growth. The impact of BMVE on seed development was also evaluated by spraying rice panicles shortly after flower fertilization and subsequently monitoring the impact on seed traits. BMVE treatment of seeds 24 h after imbibition consistently improved wheat and rice seedling shoot and root growth in lab conditions. Treated wheat seedlings grown to maturity in a greenhouse also resulted in higher biomass than controls, though only under drought conditions. Treated seedlings had increased levels of transcripts involved in reactive oxygen species scavenging and auxin and gibberellic acid signaling. Conversely, several genes associated with increased reactive oxygen species/ROS load, abiotic stress responses, and germination hindering processes were reduced. BMVE spray increased both fresh and mature seed weights relative to the control for plants exposed to 96 h of heat stress. BMVE treatment during seed development also benefited germination and seedling growth in the next generation, under both ambient and heat stress conditions. The optimized experimental conditions we developed provide convincing evidence that BMVE does indeed have efficacy in plant growth enhancement. The results advance our understanding of how STAs work at the molecular level and provide insights for their practical application to improve crop growth.

The Effects of Storage Conditions on Seed Deterioration and Ageing: How to Improve Seed Longevity

Seeds are classified as either: orthodox, seeds that tolerate dehydration; recalcitrant, seeds that are high in moisture content and cannot withstand intensive desiccation; or intermediate, seeds that survive dehydration but die during dry storage at low temperatures. Seed lifespan depends on the seed category and also varies from one species to another. The rate of loss of vigor and viability of orthodox seeds depends mainly on temperature and seed moisture content (MC); the lower the MC and storage temperature, the longer the longevity. Ultimately, storage in liquid nitrogen or seed ultra-drying by well-adapted processes should allow for long-term storage. The ageing of orthodox seeds is associated with numerous forms of cellular and metabolic damage (membrane integrity, energy metabolism, and the impairment of DNA, RNA, and proteins) in which reactive oxygen species play a prominent role. Interestingly, priming treatment can reinvigorate aged seeds by restoring the antioxidant systems. The storage of recalcitrant seeds is very difficult since they must be placed in a wet medium to avoid dehydration and at temperatures low enough to prevent germination but warm enough to avoid chilling injury. A better understanding of the mechanisms involved in ageing is necessary to identify markers in order to estimate seed longevity.

Radicle emergence and seed imbibition tests as estimators of seed vigour in mexican Pepitilla maize landrace

Seed vigour of Mexican native maize was evaluated in 10 Pepitilla populations (PEP) and three improved varieties by radicle emergence vigour (REVT) and seed imbibition tests, in order to design a plant breeding programme to obtain native varieties with high seed vigour. As part of the REVT, protrusion percentage (PR), radicle emergence (RE), non-germinated seeds (NGS) and radicle length (RL) were measured. Additionally, the imbibition curve and the percentage of testa rupture were recorded. Radicle protrusion in Pepitilla seed lots was 73-94%; for improved varieties, it ranged from 69 to 94%. For ‘PEP-2401NS’, ‘PEP-2402AG’, ‘PEP-2406GU’ and ‘PEP-2410RP’, RE was equal to PR, so, neither of these genotypes produced a radicle < 2 mm; while, RE in ‘V 236P’ was 50% lower than PR. In addition, the R2 of 0.9630 between RE and standard germination suggests that RE can be considered a reliable test to predict maize seed germination. For NGS, ‘PEP-2402AG’ and ‘H-565’ showed 6%, and RL in Pepitilla was three times greater than for the improved varieties. After 36 hours imbibition, an improved variety registered the highest water absorption (49.8%), but all Pepitilla landraces showed higher testa rupture. In general, the Pepitilla populations had a high seed-vigour due to the rapid RE and a greater RL.

Peptidomics analysis reveals stress response proteins involved in the establishment of seed vigor in tobacco

ABSTRACT: Seed vigor of tobacco (Nicotiana tabacum L.) is established during seed development stage, while its regulators remain largely unknown. Here, a comparative peptidomics analysis of the developing seeds was conducted to reveal the regulators involving the establishment of tobacco seed vigor. The most significant difference of seed vigor was observed between seeds harvested at 20 and 30 days after pollination (DAP), and then the corresponding seeds were collected separately for peptidomics analysis. A total of 2932 and 2812 nonredundant peptides were identified in seeds harvested at 20 and 30 DAP, respectively. In which, 349 differentially expressed peptides (DEPs) were characterized. To explore the potential functions of these DEPs, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were further analyzed according to their precursor proteins. Most DEP precursor proteins were involved in response to abiotic stimulus, response to water, and protein processing in endoplasmic reticulum. Further, the peptides derived from the precursor proteins, such as late embryogenesis abundant (LEA) protein, heat shock protein, peroxiredoxin, and globulin proteins, may regulates the establishment of seed vigor by influencing reactive oxygen species. The results provide a foundation for further exploration of the peptides functions on the establishment of seed vigor in tobacco.