Vigor Of Seeds Research Articles

A comparative study on seed physiology and germination requirements for 15 species of Eucalyptus

Seed physiology of 15 Eucalyptus species of interest for cut foliage plantations was unknown and therefore evaluated. The viability and vigour of seeds and germination potential of 15 Eucalyptus species was determined by using a tetrazolium (TZ) staining test, and the results were compared to a germination test. In a separate experiment, seeds of each lot were subjected to either 0 or 4-week cold stratification at 4 ± 1 °C to investigate their potential stratification requirement. After stratification, seeds were then allowed to germinate at 22 ± 1 °C with 16 h lighting per day for 36 days. Seed viability and vigour were checked by evaluating % root, cotyledon and first true leaves emergence, and the speed of emergence, in the germination test. The germination percentages varied with the species. Seed stratification with the interaction of seed species lots significantly affected both viability and vigour. The seed viability of the different species ranged from 9 to 100% and 2 to 100%, for the TZ test and germination test, respectively, with a high correlation (R2 = 0.89) between the two. Physiology tests revealed that cold stratification of seed was not required for the 15 species to maximise their germination potential and growth in Irish and British climate.

Effect of late Sowing and Hydropriming Time on some Seed Vigour Attributes of Five Chickpea (Cicer arietinum L.) Lines

A set of laboratory tests was conducted in 2015, at the Central Seed laboratory Seed Administration, Ministry of Agriculture andForestry, Khartoum, Sudan, with the objective to investigate the effect of late sowing and hydro priming times on some seed vigour attributes of five chickpea lines ( DR1-27-5; DR1-44; DR1-109; DR1-114 and DR1- 115). Seeds were provided by Hudeiba Research Station, from planting at optimum sowing date (November); late sowing (January) of the harvest of season 2011/2012. The experiment was conducted in completely randomized design with four replications. Treatments were sowing dates(2), hydropriming time (4) and chickpea lines (5) Data were collected on initial moisture content, 100 seed weight, germination percentage and rate, seedling fresh and dry weights. The results indicated that late sowing had highly significant (P ≤ 0.01) effect on seed vigour by affecting seed moisture content, 100 seed weight, germination percentage and seedlingdry weight. The lines and hydropriming time showed significant (P ≤ 0.01) effect on all studied parameters. There were positive significant (P ≤ 0.01) correlation between moisture content, 100-seed weight, germination percent and seedling fresh and dry weights; but all these parameters were negatively correlated with rate of germination. Further studies are neededto investigate, in depth, the mechanism of heat tolerance and determination of the appropriate hydro-priming time for chickpea seeds

Management of Early Harvesting Time and Drying Temperature on Maize (Zea mays L.) Seeds Storability and Seeds Vigor

Management of Early Harvesting Time and Drying Temperature on Maize (Zea mays L.) Seeds Storability and Seeds Vigor

Influence of Chemicals, Botanicals and Growth Regulator Treatments on Plant Growth and Yield Attributing Traits of Lentil (Lens culinaris L.) Variety: K-75

The experiment was conducted in post graduate Seed Testing Laboratory and Field Department of Genetics and Plant Breeding, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj (U.P) during Rabi season 2020-2021, in order to standardize the suitable pre-sowing seed treatment for Lentil (K-75). Different pre-sowing seed treatments viz.,T0-Control (untreated), T1- KCL @1%, T2- KCL @ 3%, T3- KNO3 @ 1%, T4- KNO3 @ 3%, T5- Panchagavya @ 2%, T6 – GA3 @ 20ppm, T7 – Panchagavya @ 4%, T8 – GA3 @ 10ppm,T9 – Panchagavya @ 6%,T10 – Tulasi Leaf Extract @ 2%, T11 – Tulasi Leaf Extract @ 4%,T12 – Tulasi Leaf Extract @ 6% were evaluated by screening of 12 hour. It was found that all the pre-sowing seed treatments recorded the significant difference with that of control. In field condition highest field emergence percentage,yield and yielding attributes was found in T7 -Panchagavya @ 4%. Pre-sowing seed treatment with Panchagavya @ 4% followed by GA3 @ 10 ppm, Panchagavya @ 2%, KCL @ 1% significantly increased the germination and yielding attributes of Lentil. Pre-sowing seed treatments with Panchagavya (4%) and GA3 (10ppm) resulted in maximum increase in field emergence, vigour of Lentil seeds and found to be lowest in control seeds. Pre-sowing seed treatment that leads to a physiological condition that allows the seed to germinate more effectively and no costly equipment and chemical requirements could be used. Hence it is a simple method for overcoming weak germination and seedling establishment and helps to preserve agriculture and economical, non-toxic, eco-friendly sources.

Isolation of Drought-Tolerant Endophyte Bacteria From Local Tomato Plants.

<b>Background and Objective:</b> Exploration of drought-tolerant endophytic bacteria is significant to identify bacteria that can provide plant resistance to drought stress. This study aims to obtain the potential of endophytic bacteria to promote plant growth from tomato plants in dry land. <b>Materials and Methods:</b> Exploration of endophytic bacteria from healthy tomato plants in a dry and rocky land, Muna Regency, Indonesia. Selection of drought-tolerant endophytic bacteria using polyethylene glycol 6000. Selected isolates were tested to increase the viability of tomato seeds using a Completely Randomized Design (CRD). <b>Results:</b> There were 123 isolates of endophytic bacteria isolated from the roots and stems of local tomato plants in a dry and rocky land, Muna Regency, Indonesia. There were 39 (31.70%) isolates sensitive to drought, 55 (44.71%) isolates very sensitive to drought, 8 (6.50%) isolates tolerant to drought and 21 (17.02%) isolates very tolerant to drought. Dryness for the maximum polyethylene glycol concentration at osmotic pressure of -2.00 MPa. Inoculation of endophytic bacteria in local tomato seeds increased the viability and vigour of local tomato seeds compared to the absence of endophytic bacteria. Of the 21 isolates of drought-tolerant endophytic bacteria, there were 12 potential isolates in increasing the viability of local tomato seeds belonging to the <i>Pseudomonas</i> sp. and <i>Bacillus</i> sp. bacterial groups. <b>Conclusion:</b> <i>Pseudomonas </i>sp. and Bacillus sp. isolates identified the endophytic bacteria, which can be drought-tolerant and increase tomato seeds' viability.

Discriminant analysis and comparison of corn seed vigor based on multiband spectrum

Seed vigor is an important factor determining the viability of seeds, which is related to many seed-internal components. The vitality of seeds can be identified by assessing the internal information of seeds. In this study, seeds of the sweet corn variety “TuxpenoSweet” were used. Visible and near-infrared spectral ranges were assessed to explore the viability of seeds under different processing methods, and variable selection algorithms were used to optimize models. Heat damaged and artificially aged seeds were compared with normal seeds, and two spectrometers with different band widths were used to collect the spectra of seeds. Seed discrimination models were established based on full-wavelength data and effective variables were selected through competitive adaptive reweighted sampling. The discrimination accuracy of models based on both spectrometers exceeded 95%, and the discrimination accuracy of the model based on effective variables was higher than or consistent with that of the model established with full wavelengths. The experimental results corroborate the feasibility of using spectral ranges between 500–1100 nm or 1000–1850 nm to discriminate seed vigor. The model based on the effective variables extracted by competitive adaptive reweighted sampling reduces the amount of calculated data and either optimizes or maintains the accuracy of the discrimination rate. Effective variables, extracted from the spectrometer with the spectral range of 500–1100 nm, effectively reduced the test cost and the amount of data while ensuring high seed vigor discrimination accuracy.

P-075. A survey on the diagnosis and management of uteroplacental dysfunction in hypertensive disorders of pregnancy in Japan

Seed vigor is an important factor determining the viability of seeds, which is related to many seed-internal components. The vitality of seeds can be identified by assessing the internal information of seeds. In this study, seeds of the sweet corn variety “TuxpenoSweet” were used. Visible and near-infrared spectral ranges were assessed to explore the viability of seeds under different processing methods, and variable selection algorithms were used to optimize models. Heat damaged and artificially aged seeds were compared with normal seeds, and two spectrometers with different band widths were used to collect the spectra of seeds. Seed discrimination models were established based on full-wavelength data and effective variables were selected through competitive adaptive reweighted sampling. The discrimination accuracy of models based on both spectrometers exceeded 95%, and the discrimination accuracy of the model based on effective variables was higher than or consistent with that of the model established with full wavelengths. The experimental results corroborate the feasibility of using spectral ranges between 500–1100 nm or 1000–1850 nm to discriminate seed vigor. The model based on the effective variables extracted by competitive adaptive reweighted sampling reduces the amount of calculated data and either optimizes or maintains the accuracy of the discrimination rate. Effective variables, extracted from the spectrometer with the spectral range of 500–1100 nm, effectively reduced the test cost and the amount of data while ensuring high seed vigor discrimination accuracy.

Indigenous fungi from corn as a potential plant growth promoter and its role in Fusarium verticillioides suppression on corn

Indigenous fungi can suppress infection by pathogens and produce secondary metabolites that directly or indirectly affect plant growth. This study aimed to test indigenous fungi collected from corn plants as biological control agents and their effects on the viability and vigor of corn seeds. Purposive sampling method was used for sampling where soil samples taken from the rhizosphere zone, corn stem and leaf tissue from three locations namely Maros-South Sulawesi, Bone-South Sulawesi, Sigi-Central Sulawesi, Indonesia. Rhizospheric fungi were isolated from soil collected at the rhizosphere and rhizoplane using a serial dilution technique, while the endophytic fungi isolated from the leaves and stem tissues using surface sterilization method. The isolated fungi were cultured on a potato dextrose agar (PDA) medium. An antagonism test was performed using the dual culture method on PDA media with F. verticillioides as target pathogen. Pathogenicity test and the effect of fungi on corn seed germination was carried out using the blotter test method. Parameters observed were; necrotic symptoms on seedlings, growth potential, germination, growth rate, growth simultaneity, vigor index, germination rate, and time needed for 50% of the total germination. The effect of the isolated indigenous fungi on corn growth was carried out in-planta using seedling trays. The results of the blotter test and in-planta test were further confirmed by a physiological characteristic test. And assessing the fungi's ability to dissolve potassium, phosphate, and produce protease enzymes. A total of 89 fungal isolates were isolated and collected from various parts of the corn plant. Nineteen of the 89 fungal isolates showed inhibitory activity against F. verticillioides by ≥ 50% inhibition. The fungal isolates JRP 5 MRS, JRP 9 MRS, JRP 10 MRS, JRP 7 MRS, and JEDF 1B BN were selected based on the tests and showed a consistently positive effect on seed viability and vigor with a value of ≥90%. The isolates did not cause necrosis in corn, and had the ability to suppress the growth of pathogenic F. verticillioides by ≥ 50%.

Germination characteristics of maize seeds with high and low- vigour levels in response to on-farm seed priming

On-farm seed priming, which refers to soaking seeds in water, is a pre-sowing seed enhancement technique in agriculture production. This study aimed to investigate the effects of on-farm priming on the germination performance of maize seeds with different vigour levels. Two maize hybrids and four inbred lines were used as experimental materials. Soaking treatment significantly decreased the final germination percentage of low- vigour seeds and had no beneficial effects on high-vigour seeds. With longer soaking times, the reduction in germination increased and there was a positive, negative or neutral effect of water soaking on the germination index. The responses of seeds to on-farm priming varied among the different hybrid / inbred lines used and depending on the length of the treatment. Reducing imbibition damage by PEG soaking had little impact on the poor germination induced by soaking in water. Removal of the seed pericarp alleviated the negative effects of soaking on germination. This study has demonstrated, for the first time, the differential effects of water soaking on maize seeds with different vigour levels. Our results suggested that all influencing factors, such as such as seed initial physiological quality and the technological process, need to be considered to realize the full potential of priming.

Research on Detection Method of Pea Seed Vigor based on Hyperspectral Imaging Technology

Rapid and noninvasive detection methods of seed vigor, an important index to evaluate seed quality, have been the research focus in recent years. In this paper, the detection method of pea seed vigor based on hyperspectral imaging technology was researched. First, the spectral images of different vigor grade samples with artificial aging were captured, and the original spectrum was pretreated with multiple scattering correction. Secondly, SPA and PCA were used to select respective bands. Finally, PLS-DA and LS-SVM model were established to identify the seed vigor of the pea seed, based on the whole band spectrum, the characteristic bands extracted by SPA and PCA respectively. The results showed that PLS-DA and LS-SVM models are effective, but LS-SVM had better performance. Through comparison, the method using full band spectrum was more accurate, the efficiency of method using 5 characteristic bands extracted by PCA was the highest while the way of extracting the representative band by SPA was the most meaningful to this study which achieved similar accuracy to the full band with only 20 bands. The SPA-LS-SVM method afforded the recognition accuracy (100%) for modeling set and validation set used to determine the vigor of pea seeds. The overall results suggest that hyperspectral imaging technology is useful for classification of different vitality pea seeds with non-destructive manner, which can provide a basis for further development of online scoring devices

A genome-wide association study reveals that the 2-oxoglutarate/malate translocator mediates seed vigor in rice.

Seed vigor is an important trait for the direct seeding of rice (Oryza sativa L.). In this study, we examined the genetic architecture of variation in the germination rate using a diverse panel of rice accessions. Four quantitative trait loci for germination rate were identified using a genome-wide association study during early germination. One candidate gene, encoding the 2-oxoglutarate/malate translocator (OsOMT), was validated for qGR11. Disruption of this gene (Osomt mutants) reduced seed vigor, including seed germination and seedling growth, in rice. Functional analysis revealed that OsOMT influences seed vigor mainly by modulating amino acid levels and glycolysis and tricarboxylic acid cycle processes. The levels of most amino acids, including the Glu family (Glu, Pro, Arg, and GABA), Asp family (Asp, Thr, Lys, Ile, and Met), Ser family (Ser, Gly, and Cys), and others (His, Ala, Leu, and Val), were significantly reduced in the mature grains and the early germinating seeds of Osomt mutants compared to wild type (WT). The glucose and soluble sugar contents, as well as adenosine triphosphate levels, were significantly decreased in germinating seeds of Osomt mutants compared to WT. These results provide important insights into the role of OsOMT in seed vigor in rice.

Morphophysiological responses of Crambe abyssinica Hochst. lineages submitted to water deficit during flowering

Water deficit (WD) at the start of the flowering stage can negatively affect the productivity of plants. The aim was to investigate the morphophysiological strategies of two crambe lineages (FMS CR 1326 and 1307) submitted to WD during the flowering stage and their connection with the progeny's germination. Plants were submitted to WD at the start of flowering for 12 d and then were irrigated again (water resumption, WR). As a control, plants were cultivated with uninterrupted daily irrigation. Under WD, reductions were observed in the stomatal conductance, the number of xylem vessels, and the mass of grains. Positive K- and L-bands occurred, indicating lower stability and efficiency in the use of energy under WD. In the WR period, plants presented photochemical recovery. WD induced less vigorous seeds. FMS CR 1307 had the highest capacity to maintain its photochemical performance, due to alterations in water conductivity, resulting in greater seed production and vigor.

Evaluation of volatile metabolites as potential markers to predict naturally-aged seed vigour by coupling rapid analytical profiling techniques with chemometrics

Rapid volatile detection methods for seed vigour rely heavily on artificial ageing (AA), however the comparability of volatile organic compounds (VOCs) to natural ageing (NA) and practicability of the detection models were not well known. In this study, VOCs between AA and NA sweet corn seeds were compared and Partial Least Squares Regression (PLS-R) modelswere constructed based on AA to predict the seed vigour of NA. A total of 33 VOCs were identified, among which aldehydes showed the highest consistency between NA and AA. Furthermore, a AS-PLS-R model with variable importance in projection (VIP > 1) and Pearson Correlation Coefficient (r > 0.9) algorithms, which was built on 3 volatile markers: benzaldehyde monomer, n-nonanal, 1-butanol monomer, achieved the best performance (R2p of 0.901 and RMSEP of 0.050). Therefore, coupling Gas Chromatography- Ion Mobility Spectrometry (GC-IMS) with chemometrics can be an effective way to monitor and predict stored seeds vigour.

Influence of Priming with Exogenous Selenium on Seed Vigour of Alfalfa (Medicago sativa L.)

Background: Selenium (Se) is an essential trace element to higher plants, animals and humans, but low Se levels are a global public health concern. Seed priming has become a basic strategy for the production of Se-riched agricultural products, but its application is still not clear in the production of Se-enrich alfalfa, hence this study was conducted for the production of Se-enriched alfalfa by seed priming with different concentrations and time on seed vigour. Methods: Seeds were primed with 0, 0.5, 1.0, 2.0, 4.0 and 8.0 mmol L-1 of sodium selenite solution for 0, 3, 6, 9 and 12 h at 20°C and their germination percentage, germination index, seedling vigour index and mean germination time were analyzed. Result: Seed vigour of alfalfa was improved by priming with low selenium (Se) concentration (0.5 and 1.0 mmol L-1), but was inhibited by high Se concentration ( greater than 2.0 mmol L-1). Hence, it must be necessary to carefully select appropriate concentration and time for the application of Se priming in alfalfa seeds. The optimal manner of Se priming in alfalfa seeds might be at 1.0 mmol L-1 concentration for 9 h.

Germination and fungi incidence in Cerejeira-da-mata seeds at different fruit maturation levels and temperatures

The effect of different levels of physiological maturation of the fruit and temperature on the seed germination and incidence of fungi that are harmful to the seeds, becomes fundamental for the improvement of the propagation of different native fruit species. The objective of this work was to evaluate the effect of physiological maturation and temperature on the germination and fungi incidence in Eugenia involucrata DC. seeds. Three experiments were carried out. In the experiment I, the effect of four levels of physiological maturation was evaluated, visually determined based on the color of the fruit, and in the experiment II, was evaluated the effect of two levels of maturation and two temperatures (25 and 35 °C) on the germination and vigor of the seeds. In the experiment III, was investigated the incidence of fungi in the seeds at two temperatures (25 and 35 °C). The levels of maturation evaluated did not influence the percentage of germinated seeds, but they influenced seed vigor. The temperature of 25°C was suitable for seed germination. The fungi genera found on the seeds were Trichoderma , Aspergillus , Fusarium , Penicillium , Alternaria, and Colletotrichum with variable incidences depending on evaluated temperature.

Advances in the Identification of Quantitative Trait Loci and Genes Involved in Seed Vigor in Rice.

Seed vigor is a complex trait, including the seed germination, seedling emergence, and growth, as well as seed storability and stress tolerance, which is important for direct seeding in rice. Seed vigor is established during seed development, and its level is decreased during seed storage. Seed vigor is influenced by genetic and environmental factors during seed development, storage, and germination stages. A lot of factors, such as nutrient reserves, seed dying, seed dormancy, seed deterioration, stress conditions, and seed treatments, will influence seed vigor during seed development to germination stages. This review highlights the current advances on the identification of quantitative trait loci (QTLs) and regulatory genes involved in seed vigor at seed development, storage, and germination stages in rice. These identified QTLs and regulatory genes will contribute to the improvement of seed vigor by breeding, biotechnological, and treatment approaches.

Assessment of rice seed vigour using selected frequencies of electrical impedance spectroscopy

The electrical impedance spectra of fresh and naturally aging rice seeds were measured to develop a non-destructive method for measuring the vigour of such seeds. To this end, three frequencies were selected using the coefficient of variation method within various frequency domains. A prediction model was then established using Fisher linear discriminant analysis, with the impedance, phase angle, and three-dimensional size of the seeds at the selected frequencies as independent variables and fresh and aging seeds as dependent variables. The water states and micromorphology of the seeds were subsequently evaluated to reveal the influence mechanism of impedance and vigour. The results showed that the impedance parameters of fresh and aging seeds were distinguishable, and the difference was evident in the low-frequency region. Moreover, the discriminant analysis model showed good predictive ability; the accuracies of the discriminant functions, Y 1 and Y 5 , were 90%. The impedance variables had a significant influence on the accuracy of the model, and the prediction accuracies for the fresh and aging seeds were improved by 24% and 14%, respectively, through introduction of the independent variables. Additionally, changes in the vigour, water state, and impedance were observed to be interrelated. The moisture content of the aging seeds was mainly affected by the content of bound water, and the ratio of bound water was inversely proportional to the impedance value. The variation in the impedance of the aging seeds was deemed to result from the combined effect of water state and micromorphology. • Electrical impedance spectroscopy parameters can reveal vigor level of rice seeds. • Moisture content of aging seeds is mainly affected by the content of bound water. • Ratio of bound water is inversely proportional to the impedance value. • Impedance variation combined effect of water state and micromorphology.

Hydroelectrostatic hybrid priming stimulates germination performance via ABA and GA regulation: New promising evidence for tomato gene expression

Seed companies and farmers constantly demand seeds with proper agricultural performance. Seed priming technology is a feasible method to efficiently increase the production of agricultural crops by improving the morphophysiological pattern and metabolism of important enzymes, regulating phytohormones and reprogramming gene expression. The present study reports the ability of hydroelectrostatic hybrid priming, an ecofriendly combined method with multiple priming treatments, to enhance the germination indices and vigor of tomato seeds, as well as the effects of priming over a period of time. The dynamic levels of abscisic acid and gibberellins and the transcription levels of eleven genes associated with germination were tracked during the entire priming procedure and seed germination. Hydroelectrostatic hybrid priming enhances the germination index and vigor of tomato seeds, whereas unprimed seeds took longer to complete the germination process. An increase in gibberellin levels and a decrease in abscisic acid levels were detected during the priming procedures, allowing for the weakening of the endosperm cap quickly after sowing. In addition, significant changes in the transcription levels of SlNCED2 and SlDELLA were observed during the priming procedures; both genes play important roles during seed germination. The present investigation also demonstrated a potential synergism associated with the priming procedures, conferring fast germination of tomato seeds via hormone regulation and the reprogramming of gene expression. Regarding the effects of priming over a period of time, the results showed an enhancement of the germination indices and vigor responses of primed seeds compared to primed and unprimed seeds without a storage period. Hence, hydroelectrostatic hybrid priming is a promising and ecofriendly protocol for seed companies and farmers to meet the high demand for tomatoes.

Effectivity of Pseudomonas fluorescens TBT214 in increasing soybean seed quality in different seed vigor

The research aims to assess P. fluorescens TBT214 as biopriming to increase seeds quality. Research conducted in Field Laboratory, Faculty of Agriculture, Halu Oleo University. Research arranged in randomized block designs. The first factor is the level of seed vigor consisting of 2 levels: V1= low vigor seeds (60-65% germination rate), and V2= high vigor seeds (86-100% germination rate). The second factor is the seed bio-invigoration technique which consists of 6 levels: B0=without seed bio-invigoration (control), B1=KNO3 1%, B2= KNO3 1% + P. fluorescens TBT214, B3= P. fluorescens TBT214, B4= P. fluorescens TBT214 + red brick powder, B5= P. fluorescens TBT214 + husk charcoal powder. (B5). So that obtained 12 treatment combinations. The variables observed in this study were (1) The total number of pods, (2) The number of filled pods, (3) the weight of 1,000 grains (g), and (4) Grain production (ton ha-1). The results showed that biopriming treatment could improve seed quality in seeds with low vigor levels or seeds with high vigor. Increasing seed quality resulted in the high potential of pod production, the weight of 1,000 grain seed, and yield. It can conclude that the biopriming treatment can improve the quality of seeds, both seeds with low vigor and seeds with high vigor.

Invigoration of Katokkon chili (Capsicum chínense Jacq.) seeds using halopriming and duration of immersion

A study was carried out to study the response of katokkon seeds to invigoration treatment using atonic substances, phosphoric acid, and KCl. This research was conducted in the form of an experiment using a randomized block design (RBD) 21. Invigoration treatments were given consisting of a combination of atonic substances, phosphoric acid and KCl with different immersion times. The results showed that the invigoration treatment package on the seeds that gave the best effect on the germination and vigor of the chili seeds was the best percentage value of sprouts, namely the package with atonic solution with a soaking time of 6 hours (75%), the percentage value of the vigor index, namely the package with long atonic solution immersion for 6 hours (95.00). Plumula length with a package consisting of a 6 hour immersion phosphoric acid solution with a plumule length (0.24 cm), a radicle length with a package consisting of 3 hours immersion atonic solution with a radicle length (0.51 cm), plant height with a package consisting of acidic solution Phosphate immersion 3 hours with high (9.49 cm), and long roots with a package consisting of a 12 hour long immersion atonic solution (14.02 cm).