Vigor Of Seeds Research Articles

Genetic Mapping for Seed Aging Tolerance Under Multiple Environments in Sweet Corn

Seed vigor significantly impacts seed production and storage. Enhancing seed vigor is a pivotal goal in sweet corn breeding, as improved seed sowing quality is crucial for agricultural development, aiding in better resilience against storage adversities and facilitating long-term germplasm preservation. In this study, a recombinant inbred line (RIL) population, including 158 families, was derived from the aging-tolerant line K62 and the aging-sensitive line K107. Utilizing SNP arrays, genotypes were identified, and a genetic linkage map was constructed. Composite interval mapping was employed to detect quantitative trait loci (QTLs) associated with five seed vigor traits, namely the seedling fresh weight (SFW), germination potential (GP), germination rate (GR), germination index (GI), and vigor index (VI), at three days after artificial aging treatment. Upon analysis, a total of 42 QTLs affecting seed vigor indices were identified over two years. Of these, six were linked to SFW, while the GP, GR, GI, and VI each comprised nine QTLs. Nine QTL clusters were identified, with significant contributions (>10%) from Loci02.1, Loci05.2, Loci06.1, and Loci10.1, ranging from 9.50% to 24.20%, 8.89% to 11.54%, 9.16% to 15.55%, and 7.54% to 17.77%, respectively. Candidate genes were explored within QTL cluster regions based on the aging-induced transcriptomic sequencing data of K62 and K107. Through Gene Ontology enrichment, gene annotation, and expression profiling clustering analyses, 12 positive candidate genes linked to seed aging tolerance were identified. This study provides a foundational understanding of the genetic mechanisms of seed aging tolerance and the innovation of an elite germplasm for seed aging tolerance in sweet corn.

Evaluation and Development Trends of Optical Detection Technology for Seed Vigor

Evaluation and Development Trends of Optical Detection Technology for Seed Vigor

Physiological quality of cowpea seeds after application of chitosan-based bioproduct

The use of biological methods in the agricultural field has instigated studies in the development of products based on biopolymers. Chitosan is a biopolymer and important alternative to the agrochemicals used in large cultures, presenting favorable characteristics to the development of the plants, to the environmental issues, and to the agricultural sector economy. Therefore, the present study aimed to evaluate the effect of the application of a chitosan-based bioproduct in the germination and vigor of cowpea seeds. A completely randomized design was adopted with four replications of 50 seeds, with the exception of the mass and length tests, carried out in five replications of 20 seeds. The chitosan-based product was tested in the concentrations of 0.0 (witness); 0.5; 1; 2.5; 5; and 10% and physiological quality parameters, emergency, vigor, leaf pigments contents and electrolyte extravasation were evaluated. The results indicated that the treatments coated with the bioproduct significantly increased the length of the seedling, shoots and radicle, shoot and seedling fresh masses, and seedlings dry mass emerged in a bed. We concluded that the application of the bioproduct, at FTSeed concentrations at 3 and 3.25%, as a coating in cowpea seeds induced the seedlings growth, acting as a biostimulant. The principal component analysis (PCA) showed a correlation between biochemical tests and germination potential in the field, with emphasis on the 5% treatment.

Metabolomic and Transcriptomic Analysis Reveals Flavonoid-Mediated Regulation of Seed Antioxidant Properties in Peanut Seed Vigor.

Peanut (Arachis hypogaea L.) is an oilseed crop grown worldwide. Flavonoids have profound benefits for plant growth and development because of their powerful antioxidant properties. Seed vigor is an important indicator of seed quality. However, how flavonoids impact seed vigor formation in large-seed peanuts is still poorly understood. Here, we profiled flavonoids, phytohormones, and transcriptomes of developing seeds of large-seed peanut varieties with low (ZP06) and high (H8107) seed vigor. A total of 165 flavonoids were identified, 51 of which were differentially accumulated in ZP06 and H8107. Lower levels of dihydromyricetin (0.28 times) and hesperetin-7-O-glucoside (0.26 times) were observed in ZP06 seeds than in H8107. All flavonoid biosynthesis structural genes were down-regulated in ZP06. The different hormone levels found in ZP06 and H8107 seeds could be associated with the expression of flavonoid biosynthesis genes via MYB and bHLH transcription factors. Dihydromyricetin could relate to ZP06's poor seed vigor by impacting its seed antioxidant properties. Thus, the presence of flavonoids in large-seed peanuts could contribute to their physiological quality and germination potential through controlling the accumulation of reactive oxygen species to improve seed antioxidant properties.

The Effect of Selenium Sources and Rates on Cowpea Seed Quality

Selenium (Se) is a beneficial element for plants and is essential for human nutrition. In plants, it plays an important role in the formation of selenocysteine and selenomethionine and in the activation of hydrolytic enzymes, which can aid in seed germination and reduce abiotic stress during germination. The objective of this study was to evaluate the effects of the application of selenium sources and rates to the soil on the physiological quality of cowpea seeds. The experimental design was a randomized block with four replications and a factorial scheme (7 × 2). Two sources of Se (sodium selenate and sodium selenite) and seven rates (0, 2.5, 5, 10, 20, 40 and 60 g ha−1) were used. Physiological characterization was carried out by first counting of germination, germination, emergence, accelerated aging, cold testing, electrical conductivity, length and dry biomass of shoots and roots. Germination after accelerated aging increased with selenate, even at higher rates, whereas selenite provided benefits at lower rates. Selenation linearly increased germination after the cold test and linearly reduced electrolyte leakage as the Se rate increased. The soil application of Se is beneficial for cowpea seed quality. Compared with those treated with sodium selenite, cowpea plants treated with sodium selenate through the soil produce more vigorous seeds. The application of 10 g ha−1 Se in the form of sodium selenate provides seedlings with faster germination and root development and is an alternative for rapid stand establishment.

Anti-inflammatory and antioxidant activity of functional lipids extracted through sustainable technologies from Mexican Opuntia ficus-indica seeds

Opuntia ficus-indica (OFI) seeds are a rich source of functional lipids, yet research on Mexican cultivars remains limited. This study evaluated the antioxidant and anti-inflammatory properties of lipids extracted through subcritical fluid and supercritical fluid extraction with carbon dioxide (SCE-CO₂ and SFE-CO₂) from Mexican OFI Villanueva and Rojo Vigor seeds with and without enzymatic pretreatment. SCE OFI Villanueva oil showed higher extraction efficiency of linoleic (45.86 mg/g), and oleic (9.86 mg/g) acids purified more than 5.47 and 1.18 times, respectively. Additionally, SCE oils exhibited the highest antioxidant potential (68 %) and anti-inflammatory activity (45 %) at the evaluated doses. In conclusion, SCE-CO₂ enhanced the extraction efficiency of unsaturated fatty acids, improving their potential biological effects, while enzymatic pretreatment did not positively impact on results, suggesting reduced extraction efficiency and bioactivity. These findings suggest that OFI seeds can serve as a valuable source of functional ingredients for the development of value-added food products.

Raffinose Priming Improves Seed Vigor by ROS Scavenging, RAFS, and α-GAL Activity in Aged Waxy Corn

Raffinose family oligosaccharides (RFOs) are known to benefit plants under stress conditions; however, the role of exogenous raffinose in seed germination remains poorly understood. In this study, we investigated the potential role of raffinose in promoting seed germination and elucidated the underlying mechanisms. The results showed that artificial aging significantly reduced the germination rate and vigor of waxy corn seeds. Conversely, exogenous raffinose significantly enhanced the germination of these artificially aged seeds. Exogenous raffinose significantly reduced the levels of reactive oxygen species (O2− and H2O2) and enhanced the activity of antioxidant enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT). Additionally, the levels of α-galactosidase (α-GAL) and raffinose synthase (RAFS) were significantly elevated in raffinose-treated aged seeds. These findings suggest that exogenous raffinose induces the expression of α-GAL and RAFS, thereby providing energy and reducing excessive reactive oxygen species (ROS), which in turn promotes the germination of artificially aged seeds. This study provides a theoretical foundation for enhancing seed vigor and extending seed longevity in crop management.

Physiological, morphological and chemical changes in pea seeds under different storage conditions

The loss of germination, viability, and vigor of seeds under storage conditions are the main causes of the need to multiply the seed material for leguminous crops. For crop establishment, seeds obtained in propagation fields are usually used, and the coating comes from the basic seed. In the case of leguminous species, the seeds quickly lose their viability, and in accordance with international regulations, for legumes, the number of seeds increases only in the first year of propagation. Therefore, the main objective of this study was to assess the effects of variations in the storage period, temperature and humidity on the morphophysiological and chemical traits of two pea seed varieties (Gloriosa and Kelvedon Wonder). The pea seeds were harvested at the end of June 2017, 2018 and 2019 and stored for 32, 20 and 8 months at T = 4 °C and H = 8%; T = 4 °C and H = 12%; T = 8 °C and H = 8%; T = 8 °C and H = 12%; and T = 22 °C and H = 65%. The results of the morphological, chemical, and biochemical analyses showed that the highest germination rate; hypocotyl length; radicle length; lipid content; dietetic fiber content; caloric value; and Ca, Mg, K, Na, Fe and Zn contents were detected in the Gloriosa and KW seeds stored for 8 months at 4 °C and 8% humidity. Analysis of the experimental data by statistical methods revealed that increasing the storage time had an individual significant negative influence only on the germination rate of both pea varieties and on the hypocotyl length and radicle length of the KW variety, while humidity and temperature variation had individual significant influences on the lipid content. The significant effects of humidity and temperature on the germination rate, hypocotyl length and root length of KW plants were also determined. For the remaining morphophysiological and chemical traits of pea seeds, the individual and combined effects of the factors were not statistically significant. Furthermore, the comparison of means using the Tukey test showed that storage conditions related to temperature and humidity generally used by farmers (T = 22 °C × H = 65%) did not significantly affect the majority of the nutritional properties of the pea seeds. However, maintaining pea seeds under these conditions for a longer period of time significantly affects seed germination and vigor.

Impact of accelerated aging on seed quality, seed coat physical structure and antioxidant enzyme activity of Maize (Zea mays L.).

Aging induces many deteriorative changes to seeds during storage like protein degradation, enzyme inactivation and loss of membrane integrity. In this study, we investigate the impact of accelerated aging on seed quality, seed coat physical structure and antioxidant enzyme activity of maize. Three genotypes African Tall, MAH 14-5 and a local landrace were selected and artificially aged for 96 and 120 h. The aging process led to a decrease in germination, vigour, and total dehydrogenase in seeds, while the electrical conductivity of seed leachates increased, indicating a decline in seed quality. Additionally, there was a variation in the microsculpture pattern of seed coats between genotypes. There was an accumulation of damage on the seed coat surface as the seeds aged and higher damage occurred in African Tall followed by MAH 14-5 and local landrace. Higher catalase, superoxide dismutase, peroxidase and polyphenol oxidase activity were reported in the seed coat of Local landrace and MAH 14-5 that resisted aging and deterioration while, lower catalase, superoxide dismutase, peroxidase and polyphenol oxidase activity was reported in African Tall seed coat that deteriorated during aging. Decrease antioxidant activity in aged seeds might be a possible cause of seed deterioration due to the accumulation of free radicals. Thus, these results clearly show the influence of seed coat structure and antioxidant activity on seed quality during aging.

GhDOFD45 promotes sucrose accumulation in cotton seeds by transcriptionally activating GhSWEET10 expression.

Cotton seed development and fiber elongation are the inseparable and overlapped development processes requiring the continuous supply of sucrose as the direct carbon source. However, little is known about the molecular mechanism of how sucrose is transported from the source tissues (leaves) into growing cotton seeds. Here, we identify the function of a sucrose transporter gene, Sugars Will Eventually be Exported Transporter 10, GhSWEET10 in cotton seed development. GhSWEET10 encodes a functional sucrose transporter, predominantly expressing in the funiculus, inner seedcoat, and endosperm during fiber elongation. GhSWEET10 RNAi plants (GhSWEET10i) accumulated less sucrose and glucose in growing seeds and that led to shorter fibers and smaller seeds, whereas GhSWEET10 overexpressed plants (GhSWEET10OE) had bigger seeds and longer fibers with more sugar accumulation during fiber elongation. GhSWEET10 gene is transcriptionally controlled by the transcription factor GhDOFD45. GhDOFD45 knockout plants (GhDOFD45-KO) possessed the phenotypes of smaller seeds and shorter fibers like those of GhSWEET10i plants. Furthermore, GhSWEET10 mainly exports the sucrose from the funiculus into developing seeds according to the mimic-analysis of sucrose transporting. Collectively, all these findings show that GhDOFD45 positively regulates GhSWEET10 expression to mainly transport sucrose from leaves into developing cotton seeds. Our findings also imply that the sucrose transport into enlarging seeds benefits fiber development, and thus GhSWEET10 can be selected as a target of breeding novel cotton varieties with larger and more vigorous seeds.

Assessing the potential of mosquito larval rearing water for enhanced tomato seedling establishment

Vigorous seedlings guarantee satisfactory production in the forward stages of the vegetation period. This study aimed to evaluate the impact of bio-based rearing water of two mosquito species (Culiseta sp. and Culex sp.) on tomato germination, emergence, and seedling quality. For this purpose, two distinct larval-rearing waters (LRW)(with diverse larval densities), and fry food-applied water were used as bio-priming agents. The findings revealed that using bio-based rearing water could enhance the vigor of tomato seeds. All Culex sp. derived LRWs had a shorter mean germination time than the control group. One Culex sp. derived larval rearing water treatment resulted in the shortest mean germination time (4.35 days), whereas one Culiseta sp. derived larval rearing water treatment resulted in the longest (6.20 days). There were no statistically significant differences in stem length but significant differences in plant length. Plant length was shorter in larval rearing water and fry food-applied water than in the control. The stem diameters of plants primed with larval rearing water were generally wider than the control. According to analyses of the plant length, stem length, and stem diameter measurements, the larval rearing water and fry food-applied water treatments may have had a reductive influence on plant length but provided significant support for thicker seedlings, which are more beneficial for seedlings. Other germination and growth characteristics (vigor index of germination, emergence percentage, mean time of emergence, vigor index of emergence, plant length, stem length, leaf width, leaf length, stem fresh weight, stem dry weight, root dry weight) did not show significant variation among treatments. Using larval rearing water as a bio-priming agent in agriculture offers several benefits. Larval rearing water enhances seed germination and vigor due to its possibly rich nutrient content and bioactive compounds, promoting faster and more uniform germination. It is an eco-friendly and cost-effective alternative to chemical treatments, supporting sustainable agricultural practices.

A seed-specific DNA-binding with One Finger transcription factor, RPBF, positively regulates galactinol synthase to maintain seed vigour in rice.

Seed vigour and longevity are intricate yet indispensable physiological traits for agricultural crops, as they play a crucial role in facilitating the successful emergence of seedlings and exert a substantial influence on crop productivity. Transcriptional regulation plays an important role in seed development, maturation, and desiccation tolerance, which are important attributes for seed vigour and longevity. Here, we have investigated the regulatory role of the seed-specific DNA-binding with One Finger (DOF) transcription factor and the rice prolamin box binding factor (RPBF) in seed vigour. RPBF modulates the transcription of galactinol synthase (GolS) and improves seed vigour. The promoter region of GolS-encoding genes from different species was enriched with DOF-binding sites, and the expression levels of both RPBF; OsGolS were found to enhance during seed development. Furthermore, direct interaction of RPBF with the OsGolS promoter has been demonstrated through multiple approaches: yeast one-hybrid assays, in planta promoter-GUS assays, dual luciferase assay, and in silico molecular docking. To assess functionality, Agrobacterium-mediated genetic transformation of rice was performed to generate the RNAi lines with reduced RPBF expression. In these RNAi lines, a reduction in both galactinol and raffinose content was observed. Since galactinol and raffinose are known contributors to seed vigour, the T2-transgenic lines were assessed for vigour and viability. For this, RNAi seeds were subjected to accelerated ageing by exposing them to high relative humidity and temperature, followed by scoring the germination and viability potential. Tetrazolium and seed germination assay revealed that the RNAi seeds were more sensitive to ageing compared to their wild-type and vector control counterparts. Collectively, this is the first report demonstrating that the DOF transcription factor RPBF controls the seed vigour through transcriptional regulation of GolS.

Evaluation of physiological quality and vigor of corn seeds coated with chitosan

Evaluation of physiological quality and vigor of corn seeds coated with chitosan

Seed and grain yield and quality of wheat subjected to advanced harvest using a physiological ripening process

This study aimed to evaluate the yield performance, physiological and nutritional quality of wheat seeds and grains subjected to times of application of a physiological ripening agent in pre-harvest. The experiment was arranged in a randomized block design and the treatments were six times of application of the physiological process of ripening in three replications. The applications were carried out at the ealy milk stage, milk stage, early dough stage, soft dough stage, hard dough stage and a control with no application. Yield, nutritional characteristics and the physiological quality of the seeds were evaluated. Analysis of variance and the Skott Knott test were performed at 5% probability. Linear correlation coefficients between pairs of traits were calculated. The application of the physiological process of ripening from the soft dough stage did not affect wheat grain yield. Wheat seed germination was not impacted when the physiological process of ripening was applied from the hard dough stage. The use of the ripening agent reduced the vigor of wheat seeds, regardless of the time of application. The presence of protein in grains was not influenced by the application of the physiological ripening agent. Starch had the greatest expression when the physiological process of ripening was applied between the milk stage and the early dough stage.

Proline Priming Enhances Seed Vigour and Biochemical Attributes of Rice (Oryza sativa L.) during Germination.

Seed vigour is a desirable trait especially for direct seeded rice (DSR) cultivation. Seeds with high vigour could improve seed germination, support seedlings in competing with weeds for water and nutrients, and improving seedling establishment throughout the early stages of crop growth. The success of DSR system which account for more 25% of world cultivation areas is highly dependent on the seed vigour and seedling establishment. Seed priming is a promising technique to improve seed vigour. Proline is an amino acid that has been well studied for its roles in plants under different environmental stress conditions. Nevertheless, the effect of proline as a seed priming agent in improving seed vigour in rice remain elusive. In this research, the effect of 24 h of proline priming at various concentrations (0 mM, 1 mM, 2 mM, 10 mM and 20 mM) on rice seed vigour, amylase activity, and total soluble sugar (TSS) content of a Malaysia indica rice variety, MR269 was investigated. Results showed that seeds primed with lower concentration of proline (0 mM, 1 mM and 2 mM) had better germination responses while priming at high concentrations (10 mM and 20 mM) reduced seed germination. Among the concentration tested, priming with 1 mM proline enhanced seed vigour with significantly higher germination percentage (GP), germination rate index (GRI) and seedling vigour index (SVI). In addition, proline primed seeds also exhibited increased amylase activity and TSS content as compared to unprimed seeds. However, priming seed with 20 mM proline was detrimental to the seed vigour and seedling growth whereby lower GP, GRI and SVI and higher mean germination time (MGT) were observed. In short, this study shows that proline could be a potential seed priming agent to improve seed vigour in rice.

Integrated multispectral imaging, germination phenotype, and transcriptomic analysis provide insights into seed vigor responsive mechanisms in quinoa under artificial accelerated aging.

Seed vigor is an important trait closely related to improved seed quality and long-term germplasm conservation, and it gradually decreases during storage, which has become a major concern for agriculture. However, the underlying regulatory mechanisms of seed vigor loss in terms of genes remain largely unknown in quinoa. Here, two cultivars of quinoa seeds with different storage performance, Longli No.4 (L4) and Longli No.1 (L1), were subjected to transcriptome sequencing to decipher the pathways and genes possibly related to vigor loss under artificial aging. Multispectral imaging features and germination phenotypes showed significantly less seed vigor loss in L1 than in L4, indicating L1 seeds having stronger aging resistance and storability. Totally, 272 and 75 differentially expressed genes (DEGs) were, respectively, identified in L4 and L1 during aging. Transcriptomic analysis further revealed the differences in metabolic pathways, especially, flavonoid biosynthesis, TCA cycle, and terpenoid backbone biosynthesis were significantly enriched in L4 seeds, while carbon metabolism in L1 seeds, which involved key genes such as CHS, CHI, AACT, ENO1, IDH, NADP-ME, and HAO2L. It indicated that the adverse effects on flavonoids and terpenoids induced by aging might be the significant reasons for more vigor loss in storage sensitive seeds, whereas storage tolerant seeds had a stronger ability to maintain carbon metabolism and energy supply. These findings elucidated the underlying molecular mechanism of seed vigor loss in quinoa, which also provided novel insights into improving seed vigor through modern molecular breeding strategies.

Interference of mechanical damage on the physiological performance of corn seeds

Corn (Zea mays L.) is cultivated and consumed globally due to its nutritional qualities and short production cycle. However, during harvesting, processing, transportation, and storage, seeds are susceptible to various types of damage that can compromise their productive capacity, affecting both germination and plant vigor. Therefore, this study aimed to evaluate the physiological quality of corn seeds subjected to different types of mechanical damage. The experiment was conducted at the Laboratory of Storage and Processing of Agricultural Products at the Federal University of Campina Grande, Campina Grande, Paraíba, Brazil. Corn seeds were subjected to four types of mechanical damage: hammering, cutting, abrasion, and perforation, alongside a control sample that did not undergo any mechanical damage. Each treatment (mechanical damage) consisted of 200 seeds, which were evaluated through germination tests, including first count, germination rate, germination speed index (GSI), seedling dry weight, and root length. The experiment was conducted in Completely Randomized Design. Data were subjected to Analysis of Variance, and means were compared using the Scott-Knott test (P ≤ 0.05) when necessary. Mechanical damage reduces the germination and vigor of corn seeds. Cutting-type damage affects corn seeds more severely, reducing germination by an average of 80% and impairing vigor.

Effect of physical energy on germination and seedling vigor of alfalfa seeds (Medicago sativa L.)

Effect of physical energy on germination and seedling vigor of alfalfa seeds (Medicago sativa L.)

Physiological quality of green corn seeds under water stress conditions

This study aimed to evaluate the physiological quality of green corn seeds subjected to water stress. The experimental design used was completely randomized, in a 2 x 5 factorial arrangement, consisting of two corn hybrids (AG1051 and BRS3046) and five osmotic potentials (0; -0.4; -0.8; -1.2 and – 1.6 MPa) simulated with aqueous solutions of polyethylene glycol 6000 (PEG 6000), with four replications of 50 seeds per treatment, totaling 40 experimental units. The analyzed variables were: water content, germination, root protrusion, final root protrusion, germination speed index, root length and coleoptile length. There was a significant effect of the interaction between hybrid factors and osmotic potentials for all analyzed variables, except for final root protrusion, which showed an isolated effect for the two factors studied. The results obtained indicated a higher percentage of germination and initial development of green corn seedlings for green corn seeds, hybrids AG1051 and BRS3046 at the osmotic potential of 0.0 MPa. The germination and vigor of green corn seeds decrease under water stress conditions from the osmotic potential of -0.4 MPa induced by PEG 6000, inhibiting the development of normal seedlings, affecting seed viability. Hybrid AG1051 presents superior germination and vigor compared to BRS3046.

QTL mapping for seed vigor-related traits under artificial aging in common wheat in two introgression line (IL) populations

Background Seed vigor recognized as a quantitative trait is of particular importance for agricultural production. However, limited knowledge is available for understanding genetic basis of wheat seed vigor. Methods The aim of this study was to identify quantitative trait loci (QTL) responsible for 10 seed vigor-related traits representing multiple aspects of seed-vigor dynamics during artificial aging with 6 different treatment times (0, 24, 36, 48, 60, and 72 h) under controlled conditions (48 °C, 95% humidity, and dark). The mapping populations were two wheat introgression lines (IL-1 and IL-2) derived from recipient parent (Lumai 14) and donor parent (Shaanhan 8675 or Jing 411). Results A total of 26 additive QTLs and 72 pairs of epistatic QTLs were detected for wheat seed-vigor traits. Importantly, chromosomes 1B and 7B contained several co-located QTLs, and chromosome 2A had a QTL-rich region near the marker Xwmc667, indicating that these QTLs may affect wheat seed vigor with pleiotropic effects. Furthermore, several possible consistent QTLs (hot-spot regions) were examined by comparison analysis of QTLs detected in this study and reported previously. Finally, a set of candidate genes for wheat seed vigor were predicted to be involved in transcription regulation, carbohydrate and lipid metabolism. Conclusion The present findings lay new insights into the mechanism underlying wheat seed vigor, providing valuable information for wheat genetic improvement especially marker-assisted breeding to increase seed vigor and consequently achieve high grain yield despite of further investigation required.