Viewer Program Research Articles

Analysis of Class II Intermaxillary Elastics Applied Forces: An in-vitro Study

ObjectivesThe aims of this study were: (1) to assess the average inter-arch distances characterizing Class II malocclusions, (2) to analyze the applied forces at those distances by different elastics, and (3) to compare measured forces with those declared by manufacturers, both in dry and wet environments.Materials and MethodsSettings and sample population: Class II models of 167 adult subjects (96 women and 71 men, age: 28 ± 3 years) referred to the Orthodontic Department of the University of Turin, Turin, Italy, between January 2018 and January 2020, were collected. Distances between facial axes (FA) points of upper canines and lower first molars (A), upper first premolar and lower first molar (B), upper second premolar and lower first molar (C), upper canine and lower first premolar (D), and upper canine and lower second premolar (E), were measured using 3Shape Ortho® Viewer program. Different elastics' diameters and forces were tested at those distances. The MTS Insight® Electromechanical Testing System was used to measure the tensile forces of elastics. The applied forces were measured in dry (T0) and wet conditions, after 1 (T1), 6 (T2), and 12 h (T3).ResultsAverage distances were calculated: A = 24.64 mm (SD 2.10), B = 16.3 mm (SD 1.94), C = 9.78 mm (SD 1.77), D = 9.8 mm (SD 1.88), and E = 15.99 mm (SD 2.06). Significant differences (p < 0.05) were highlighted between the measured force and the force declared by manufacturers, and all elastics had a significant force decay (p < 0.05).ConclusionThe results showed that 3/16" 4.5 oz are the most reliable elastics in terms of applied force with respect to the declared one and in terms of force degradation.

Online education research trends in science education: content and bibliometric mapping analysis

This study aims to reveal online education research trends in science education through content analysis and to examine the bibliometric structure of articles on online education in science education. A total of 68 articles were accessed from the Web of Science database for bibliometric mapping analysis, and all articles published without a year limit were included for content analysis. The data set was analyzed in the Web of Science database and the VOS viewer program. Science education, e-learning, distance learning, and online education were found to be the most used keywords in 71 publications produced between 1992 and 2022. Among these keywords, it stands out that e-learning is used in more recent publications. When the distribution of articles by year is examined, it is seen that there was an increase in publications according to the years, but the peak is in 2020 and 2021 due to the impact of the COVID-19 pandemic. The most cited journals were Computers & Education, Science Education, Journal of Research in Science Teaching, and International Journal of Science Education.

Diyabetik Ayak Enfeksiyonu İle İlgili Yayınların İşbirliğine Dayalı Ağ Analizi ve Bibliyometrik Analizi

Aim: As the number of people with diabetes mellitus is increasing globally, its consequences are worsening. People with diabetes are prone to foot problems that develop due to prolonged periods of high blood sugar levels diabetic foot disease (DFD) results in a major global burden for patients and the health care system. It is important to fully understand the characteristics of DFD, which is a major cause of mortality, for early diagnosis and definitive treatment. Our study aimed to examine DFD studies and guide the studies to be planned in the future.
 Materials and methods: In our study, the Web of Science (WOS) database was scanned to search for publications until February 28, 2021. While the data obtained in our study were analyzed to present various bibliometric indicators, the maps were visualized using the VOS viewer program (VOS viewer). The Hirsch (H) index and impact factor (IF) of the publishing journal were used as indicators of the impact of the publications. The publications containing the keywords 'Diabetic foot' and 'Diabetic foot disease' or 'Diabetic foot infection' were examined.
 Results: A total of 802 publications were reached from the WOS database according to the search criteria. The most publications were from the USA (32.941%), Turkey (9.412%), and England (8.431%). Most of the publications were articles (63,6%) and meeting abstracts (16,9%). 19,4% of the publications were in the endocrinology and metabolism area. The average number of citations of these 802 publications was found to be 20,98. The H index was found to be 53. It was determined that the number of citing rates of publications on diabetic foot infections has increased over the years, however, it was determined that the number of publications on this subject decreased over the years.
 Conclusion: This is the first bibliometric study on DFD. Bibliometric analysis should be used more widely to evaluate the quality of the increasing academic paper and guide the missing study issues.

Bacteria as an Efficient Bacteriosystem for the Synthesis of Nanoparticles: A Bibliometric Analysis

The growth trend of publications in the field of nanoparticles biosynthesis by bacteria was analyzed using bibliometric techniques to the identification of the areas with significant development and the orientations that have guided the research on bacteria. This study extracted data from the Clarivate Analytics Web of Science (WoS) and Scopus between 2000 and 2020 to compare results. The number of articles published annually, participating countries, resource growth rate, most used keywords and research collaborations were obtained from bibliometric analysis. The results showed that nanoparticle documents in materials science, biochemistry, genetics and molecular biology have improved citation rates as the authors researched in multidisciplinary areas. Although these keywords have the most sites, they are not emerging keywords, and their most common use was in the years 2014–2018. The results showed the upward trend of articles published from 2000 to 2020, most of which were related to the last 10 years. The 5 countries that had the most articles published in this field were China, USA, India, Germany and Iran. The top sources that had the most published documents in this field had a similar scope to our research. The results of each section had a significant relationship with other sections of this study. VOS viewer program was used for quantitative analysis. It seems that bibliometric methods will complement meta-analysis and qualitative structured literature reviews as a method for reviewing and evaluating scientific literature. The advantage of the bibliographic analysis was the awareness of trends in a study area.

Bibliometric Analysis of Peer Reviewed Literature on Sustainable Agriculture Research – Challenges and Prospects

With the need to fulfil the rising food demands of the increasing population, the agriculture sector faces a huge challenge. The issue is compounded by climatic change, severe shortage of water, increasing salinity, habitat vulnerability and pollution amongst others. Considerable emphasis worldwide is focused on research aimed to sustain as well increase agricultural productivity. The present study focuses on systematically mapping the thrust areas of research related to sustainable agriculture based on search results from SCOPUS database. The retrieved data was analysed using VOS viewer application program to create network maps based on term co-occurrence analysis. The analysis further provides insight into the most active authors, countries, institutions as well as citations. A total of 12,261 documents were retrieved. A significant increase in the number of publications was seen in the last decade. The retrieved documents were contributed by authors belonging to 156 countries and had an average of 6.24 citations per document with an h-index of 40. Cluster analysis highlighted three focus areas of research- (1) sustainable agriculture (2) factors affecting crop yield and (3) basic research into plant growth, metabolism, development and aging. The underlying principle of all the research programs pertain to the use of innovative technologies and judicious use of resources for maximal production with due importance to environmental, economic and social aspects.

Linkages between Climate Change and Coastal Tourism: A Bibliometric Analysis

Tourism and climate are inextricably linked to several interactions. In recent years, there has been a greater focus on the linkages between climate change and coastal tourism. This study aims to provide an overview of the literature on climate change and coastal tourism, identify core areas of interest and important sources and authors, and examine the thematic evolution of the field. A bibliometric analysis of 92 documents related to climate change and coastal tourism published in the Web of Science Core Collection database was carried out. The analysis provides information on the most cited papers, most leading authors, the most productive countries, and the most leading institutions in this field. The study utilized the Visualization of Similarities Viewer program (VOS) to map author keyword co-occurrences, co-citations, and bibliographic coupling. The study showed that, with some fluctuations since 2008, the number of publications in this field had increased significantly. The most influential authors and most productive institutions are from the United States of America, England, Canada, and other European countries. The findings of this study will assist researchers conducting climate change and coastal tourism-related studies to understand which papers, academics, organizations, countries, and journals have a dominant influence on climate change and coastal tourism research.

Etik Girişimcilik Alanında Yapılmış Ulusal ve Uluslararası Çalışmaların Bibliyometrik Analizi(The Bibliometric Analysis of National, and International Studies on Ethical Entrepreneurship)

Purpose – In the study, it is expected to provide an overview of the related publications by examining the studies on ethical entrepreneurship in the national and international literature, to provide information about network structures, to enable the monitoring of developments and changes in the field as well as to shed light on future studies in order to spread ethical awareness in entrepreneurship.. Design/methodology/approach – Studies on “ethics” and “entrepreneurship” on the subject through TR Index Turkish Academic Network and Information Center (ULAKBIM) until 2021 and similarly on “ethics” AND “entrepreneurship” (in English) on the subject published under the SCI, SSCI and AHCI citation indexes through Web of Science database were analyzed. As a result of the research, using social network analysis via VOS Viewer program for international publications institutions and author collaborations were presented in maps. In the study, a total of 313 works in national and international literature were examined using the bibliometric citation analysis technique, one of the qualitative research methods. Findings – Based on the studies published in the Ulakbim TR index, it is seen that the issue of ethics and entrepreneurship has not been studied sufficiently in the national literature. In the Web of Science database, there is a remarkable increase in the number of studies in 2013, and later due to reasons such as journals particularly devoted to the ethics subject, and technological developments. When compared with the studies in the international literature, it is noteworthy that the social entrepreneurship dimension of the subject is not discussed in the national literature. In addition, in terms of citations, the fact that the number of citations to national studies is quite low compared to international ones reveals the low level of awareness. Discussion – The analysis shows that the issue of ethical entrepreneurship has not been sufficiently covered in national studies yet and that it is insufficient to determine the connection between the issue of ethics and entrepreneurship. The academic community needs to support and contribute to the development of ethical entrepreneurship with scientific data through researches at micro, meso and macro levels. So that, those studies will draw the attention of organizations to focus on ethical entrepreneurship in challenging Red Ocean conditions for their sustainability

A Bibliometric Mapping of the Research Trends of Public Governance and Entrepreneurship Framework

The involvement of governments and public institutions in entrepreneurial activity has grown considerably in the last years. Public governance has begun to pay more attention to the fact that, in order to achieve the goal of economic development in a country, it is necessary to increase entrepreneurship both qualitatively and quantitatively. Most governments have created significant actions that encouraged and supported the entrepreneurial activity and also they have contributed to supporting the private sector in the economy. The present paper analyses the scientific works related to entrepreneurship and public governance, as well as to investigate the connection between these topics. The study presents a bibliometric analysis of the importance of the nexus public governance and entrepreneurship. The proposed research studies 201 references collected from the Clarivate Analytics, Web of Science Core Collection database. The research methodology performed to graphically map the literature is bibliometric analysis. The analysis is carried out on three distinct levels, namely the analysis of the co-occurrence of keywords, co-citation and co-author by means of (VOS) viewer program. This approach allows us to highlight the main results presented in journals, magazines, documents, subjects, authors, institutions but also results recorded at the country level. The study also evidence the main trends and impact of research in the form of a visual and schematic research framework on the relationship between public governance and entrepreneurship and its related elements, namely innovation and development.

Correlation between the transverse dimension of the maxilla, upper airway obstructive site, and OSA severity.

Acquiring a better comprehension of obstructive sleep apnea physiopathology can contribute to improving patient selection for surgical treatments. We hypothesize that maxillary transverse deficiency restricts the space available for the tongue, leading to upper airway obstruction during sleep. Our primary hypothesis was that maxillary transverse deficiency increases the prevalence of tongue collapse during drug-induced sleep endoscopy (DISE). The secondary hypothesis was that maxillary transverse deficiency will also increase the prevalence of circumferential collapse at the velopharynx. The exploratory hypothesis was that maxillary transverse deficiency is associated with increased obstructive sleep apnea severity. The objectives of this study were to correlate maxillary morphometric measurements with (1) the anatomic level of obstruction during DISE and (2) the apnea-hypopnea index on polysomnography. We made a cross-sectional analysis of patients with obstructive sleep apnea undergoing DISE in search of positive airway pressure alternative treatment. Maxillary measurements were collected from a computed tomography scan (interpremolar distance, intermolar distance [IMD] and sella-nasion A point angle), findings from DISE, and sleep study variables from polysomnography. Correlation between computed tomography, DISE, and polysomnography data was assessed using Pearson's correlation, and receiver operating characteristic curves were determined for each facial measurement. Sixty-nine patients were included in the study. The group with velopharyngeal circumferential collapse had mean IMD = 26.30 mm (25.5-31.45), and the group with anteroposterior collapse had mean IMD = 29.20 mm (26.8-33.10; P = .040). The group with complete tongue-base obstruction had mean interpremolar distance = 26.40 mm (25.1-28) and IMD = 26.30 mm (25.6-28.4), and the group without obstruction had mean interpremolar distance = 28.7 mm (27.2-30; P = .003) and IMD = 34.06 mm (32.1-37; P < .001). The receiver operating characteristic curve determined an IMD cutoff of 29.8 mm for predicting tongue-base obstruction. The maxillary transverse deficiency, identified by reduction in interpremolar distance and IMD, predicted the occurrence of complete tongue-base obstruction, complete concentric collapse at the velopharynx, and multilevel obstruction during DISE. We did not find an association between the maxillary measurements and obstructive sleep apnea severity. These associations hold some promise in ultimately supplanting insights previously available only through DISE.

Transcriptome-based single-nucleotide polymorphism markers between Pinus brutia and Pinus halepensis and the analysis of their hybrids

Natural hybridization occurs in sites where Pinus halepensis Mill. and Pinus brutia Ten. overlap geographically. Studies have shown that these hybrids demonstrate vigorous growth. Currently, there is no efficient method for hybrid identification. In the present study, a transcriptome database that was established from P. halepensis and P. brutia provided 111,388 potential SNP markers for hybrid identification. We selected 295 SNPs that were verified in silico using the Integrative Genomics Viewer (IGV) program. Forty-three SNPs were confirmed in vitro using several methods, including CAPS, HRM, TaqMan, KASP, and direct sequencing. Seven SNPs that were used for genotyping P. brutia trees in three planted sites along the climatic gradient of Israel demonstrated hybrid incidences of 2.5, 6.3, and 9.4%. The trunk volume of the hybrids in these sites was 2.9, 2.4, and 1.2 times larger than that of their P. brutia neighboring trees. Differences among the sites in the hybrid vigorous growth extent might have resulted from several factors, including genetic source, stand age, thinning history, and site conditions. However, the overall vigorous growth of the hybrids that was more pronounced in the arid site highlights the potential of interspecific hybridization as a means for improving the drought resistance of forest trees. This work provides a reliable SNP database for the identification of P. brutia × P. halepensis hybrids for the potential utilization of these hybrids to confront changes in climate.

Analysis of the phenotypic heterogeneity of CD123-positive cells in Kikuchi-Fujimoto disease using a sequential immunoperoxidase labeling and erasing method

To identify the phenotypic heterogeneity of CD123-expressing cells in the affected lymph nodes in patients with KFD by a sequential immunoperoxidase labeling and erasing (SIMPLE) method. Excision biopsies of lymph nodes were examined in 3 patients with KFD. After an immunohistochemical reaction using a single antibody, the tissue specimen was digitized with a Pannoramic 250 Flash III scanner (3DHISTECH, Hungary), then the cover glass was removed from the section, the specimen was hydrated and placed in a specialized buffer. Then the following primary antibody was applied to the washed tissue specimen and further immunohistochemical reaction and scanning were performed. As a result, each tissue specimen was sequentially stained in reactions with 4 antibodies. The microphotographs of specimens stained in a reaction with anti-CD123 antibody showed positive cells for their identification in the Pannoramic Viewer program (3DHISTECH, Hungary) on the remaining microphotographs displaying the expression of the other 3 markers. The selected fields of view were exported to a JPG format. Assessing the co-expression of the antigens CD123, MNDA, CD68, and TCL1A detected 4 CD123+ cell subpopulations: No. 1. CD68+/ MNDA+/ TCL1A+; No. 2. CD68+/ MNDA+/ TCL1A-; No. 3. CD68+/ MNDA-/ TCL1A+; No. 4. CD68-/ MNDA-/ TCL1A+. SIMPLE has shown the phenotypic heterogeneity of CD123-positive cells (some of them may be PDCs) and could identify 4 immunophenotypically distinct subpopulations in the affected lymph nodes in patients with KFD. Further investigations are needed to define the role of subpopulations in the pathogenesis of KFD and other diseases.

СЕМАНТИЧЕСКОЕ УПРАВЛЕНИЕ КАК ИНСТРУМЕНТ СТРАТЕГИЙ НАУЧНО-ТЕХНОЛОГИЧЕСКОГО РАЗВИТИЯ

The paper suggests that semantic events in the field of science regulation correlate with the processes of implementation of the global project, conventionally called «Industry 4.0». It is shown that the Russian Strategy of scientific and technological development is implemented to a certain extent with the help of methods and means of semantic management. The Google Ngram Viewer program is used in order to compare the frequencies of management concepts usage. Under the conditions of increasing crisis phenomena, big challenges and sanctions pressure, a multi-level approach should replace gamification and experimental methods in the Science and Technology Development regulation, when changes are carried out sequentially, from one level (organization, social structure, conceptual understanding) to another.

Морфогенез енхондральних осередків окостеніння кісток пташенят спортивних голубів у ранньому постнатальному періоді онтогенезу

Racing pigeons are potential carriers of zooanthroponic diseases, since they can travel considerable distances during their flight. The study of the formation features of the pigeon skeleton’s hematopoietic function on different levels of its structural organization with the determination of the relationship between the growth and development of hematopoietic components and the processes of osteohistogenesis is necessary to understand the immunity of pigeons, especially during the early stages of their development when young animals are in the greatest risk of disease, especially during processes of osteogenesis and haematopoiesis in chicks. The bones of the axial and peripheral skeleton of one-day-old, 5-, 10-, 15-, 20-, and 25-day-old pigeons (n = 5) were examined. The presence and degree of development of dia- and epiphyseal ossification centers (EOCs), their relative area (RA) and X-ray density in the organs of universal hematopoiesis were determined on radiographs made on an x-Ray-TW-102 x-ray machine with an Alpha 4600 receiver using the MultiVox Dicom Viewer program. It was established that in day-old pigeons the rudiments of the studied bones are completely built by cartilaginous tissue, have low radiographic density, and fuzzy contours on radiographs. On the 10th day of life, the axial skeleton of pigeon cubs has formed EOCs of head and a tubercle on the 5th rib (third «true rib»), while in the bones of the peripheral skeleton there is an enchondral EOC of the diaphysis. The spongy and compact bone structure (BS) of the bones were fuzzy. The body of the rib and the epiphyses of the limbs’ tubular bones were cartilaginous. The X-ray density of the humerus at this age remains unchanged, and the tibial-metatarsal increases by 1.5 times, reaching 12 HU. In 15-day-old chicks, the RA of the EOC in the skeleton was moderately increased due to an increase in the RA of previously formed EOCs at the age of 10 days, the appearance of individual EOCs in the body of 5th rib, and in the tubular bones of the limbs, the enchondral EOCs of the proximal and distal epiphyses. In the diaphysis of the extremities tubular bones, a strip-like compact BS was clearly distinguished, and in the epiphyses, a small-sized spongy BS. The X-ray density of the bones almost doubled, reaching 19–21 HU. In 20-day-old pigeons, the processes of osteohistogenesis in the skeleton were almost completed, in the studied bones, all the main and additional EOCs were well expressed, their RA increased sharply. X-ray density of bones did not change. For 25-day-old pigeons in the skeleton, there was a slight increase in RA of the EOC, a process of complete synostosis of the bones was characteristic. From the moment of the appearance of the EOC in 10-day-old chicks until they reach the age of 25 days, the bones of the axial skeleton and the skeleton of the limbs were formed by 95–100% of BS, and their X-ray density was increased almost 2.5 times, reaching 19–26 HU.

Green competitiveness: the evolution of concept formation

The modern tendencies confirmed the necessity to consider the environmental factors in companies' effectiveness. The goal of the article is to investigate the structural and functional environment of the development of the green competitiveness concept. In the paper were used the methods of bibliometric analysis for determining vectors of green competitiveness concept development. The hypothesis of the study is the growing tendency of analysing sustainable competitiveness by scientists. In this order, a bibliometric analysis of the spread of the concept of «green competitiveness» in scientific articles indexed by the Scopus database was performed. The authors reviewed about 30000 papers from Scopus base in the period from 1996 till 2019. The Scopus screening tools showed that in 2004 the number of articles that focused on green competitiveness began to increase. On the international level, the investigated area was represented by the next countries: the United States, China, India, Great Britain, Taiwan. Using the VOS viewer program were identified the main clusters of the scientific papers, which have investigated green competitiveness with the interconnection of such categories: green marketing, green innovation and sustainable development. The findings could be used for improving the basis for the creation of the strategies for green competitiveness promotion.

Promising Modern Research Areas in Smart Mobility

Contemporary smart mobility technology makes it possible to solve the problems of urban transport, improving quality of urban mobility, increasing availability of transport, and ensuring its efficiency. The objective of the study is to identify key trends of research on smart mobility in recent scientific sources. The author, using VOS Viewer program, carried out a bibliometric analysis of publications indexed in the Web of Science and Scopus databases and containing keywords related to smart urban mobility. Co-citations, cross-references, shared keywords, co-authorships have been analyzed for the entire set of selected publications. As a result, a map of links between publications in the field of smart mobility was built, defining existing relationships between keywords and authors in this area. Also, a map of clusters was built , which displays proximity of publications to each other. A cluster comprises a group of research closely related via research topics, authors, and keywords. Clustering of publications was conducted followed by identifying six clusters, each of which reflects single promising research area in the field of smart mobility. The results obtained make it possible to identify promising development trends for smart mobility through automated processing of a highquality array of publications. The results can be used by municipal officials, transportation business and academia to review and analyze emerging smart mobility technologies and to make informed urban transport planning decisions.

Analysis and modification of graphic data compression algorithms

The article examines the algorithms for JPEG and JPEG-2000 compression of various graphic images. The main steps of the operation of both algorithms are given, their advantages and disadvantages are noted. The main differences between JPEG and JPEG-2000 are analyzed. It is noted that the JPEG-2000 algorithm allows re-moving visually unpleasant effects. This makes it possible to highlight important areas of the image and improve the quality of their compression. The features of each step of the algorithms are considered and the difficulties of their implementation are compared. The effectiveness of each algorithm is demonstrated by the example of a full-color image of the BSU emblem. The obtained compression ratios were obtained and shown in the corresponding tables using both algorithms. Compression ratios are obtained for a wide range of quality values from 1 to ten. We studied various types of images: black and white, business graphics, indexed and full color. A modified LZW-Lempel-Ziv-Welch algorithm is presented, which is applicable to compress a variety of information from text to images. The modification is based on limiting the graphic file to 256 colors. This made it possible to index the color with one byte instead of three. The efficiency of this modification grows with increasing image sizes. The modified LZW-algorithm can be adapted to any image from single-color to full-color. The prepared tests were indexed to the required number of colors in the images using the FastStone Image Viewer program. For each image, seven copies were obtained, containing 4, 8, 16, 32, 64, 128 and 256 colors, respectively. Testing results showed that the modified version of the LZW algorithm allows for an average of twice the compression ratio. However, in a class of full-color images, both algorithms showed the same results. The developed modification of the LZW algorithm can be successfully applied in the field of site design, especially in the case of so-called flat design. The comparative characteristics of the basic and modified methods are presented.

Effect of Hemodialysis on Peripapillary Choroidal Thickness Measured by Swept-Source Optical Coherence Tomography.

The aim was to investigate the effects of hemodialysis (HD) on peripapillary choroidal thickness (PCT) by swept-source optical coherence tomography and on other ophthalmologic parameters in patients with end-stage kidney disease. This was a prospective observational study. The authors evaluated 29 patients who underwent HD for end-stage kidney disease. Detailed ophthalmologic examinations and swept-source optical coherence tomography were performed immediately before and after HD. PCT was measured using the modification tool in the built-in OCT image viewer program. Changes in PCT before and after HD were statistically analyzed. The average PCT significantly decreased from 127.3±49.2 μm before HD to 117.1±50.9 μm after HD (P<0.001). A significant correlation was found between changes in PCT and macular choroidal thickness (ρ=0.547, P=0.002). Changes in mean ocular perfusion pressure did not significantly correlate with changes in PCT (ρ=-0.049, P=0.803). PCT significantly decreased after HD. HD could influence the optic nerve head and its surrounding structures.

Proteomic Examination for Gluconeogenesis Pathway-Shift during Polyhydroxyalkanoate Formation in Cupriavidus necator Grown on Glycerol.

Because of availability and inexpensiveness, glycerol can be considered as a suitable raw material for polyhydroxyalkanoate (PHA) production with bacterial fermentation. Nevertheless, compared to the production of glucose as a raw precursor, PHA produced from glycerol by Cupriavidus necator was found to produce lower PHA with low bacterial growth rates. According to our study, C. necator was able to synthesize glucose-like intermediates from glycerol via gluconeogenesis. This resulted in a decrease of the cell dry weight and the yield of PHA polymers, especially in the active cell growth phase. It was indicated that glycerol used as a carbon source of the PHA synthesis pathway has glucogenesis-shift, which causes a decrease of the PHA content and productivity. In this research, we investigated the proteins that were closely expressed with the increase of the intracellular PHA and glucose content. For solving the above problem, the proteins inside the bacterial cells were analyzed and compared to the database proteins via mass spectrometry. The proteins were isolated by 1-D SDS-polyacrylamide gel electrophoresis (PAGE) technique and identified by the liquid chromatography mass spectrometry (LC-MS) technique. By using bioinformatics validation, a total number of 1361 proteins were examined and found in the culture bacterial cells. Selective protein expression was correlated with the amount of PHA at each cultivation time and generating glucose by studying the 1361 proteins was elucidated in proteomic information. The results of the cluster of proteins were found to contain 93 proteins using the multiple array viewer (MEV) program with the KMS data analysis model. Protein species with the same expression pattern for PHA and six proteins with similar expression patterns were found to be correlated with generating glucose content. The associations of the two protein groups were then determined through a Stitch program. The protein and chemical associations were analyzed both directly and indirectly through different databases. The proteins of interest were found with research data linked between glycerol and glucose. Five protein types are connecting to glucose and glycerol shift pathway, two of which are glycosyl hydrolase (H16_B1563) and short-chain dehydrogenase (H16_B0687), both of which are enzymes used to break the bonds of complex sugars, possibly related to the partial conversion of glycerol to glucose. The two proteins found in the strains used in the Cupriavidus necator H16 experiment give rise to the break down the bonds of α,α-1,1-glucoside of malto-oligosyltrehalose and short-chain sugar molecules such as mannitol (C6H14O6), respectively. In this research, finding the associated expression proteins which is involved in changing the pathway of gluconeogenesis shift to PHA synthesis will be useful information on genetically modifying microorganisms to produce PHA more efficiently, leading to reduction of the production costs.

Anode heel effect application with step wedge against effect of signal to noise ratio in computed radiography

It was researched on the application of Anoda Heel Effect (AHE) with a step wedge on the effect of Signal To Noise Ratio (SNR) on Computed Radiography (CR) has been carried out. This research was conducted on a 21 step wedge with two treatments, namely the application of AHE and without the application of AHE. This measurement is repeated three times on radiographs to obtain a total image of six images (without the application of AHE as many as three images and with the application of AHE as many as three images). The results of taking radiographs using AHE and without AHE were measured using the RadiAnt Dicom Viewer program. The SNR value on the step wedge image without the AHE application has an average of 26.89. The SNR value on the step wedge image using AHE is 60.54. The results of the correlation test (Pearson correlation test) on the SNR showed that there was a significant and very strong effect of the application of AHE on the step wedge on the SNR in CR (p-value &lt;0.001 and the R-value ranging from 0.600 to 0.799).

Development of Interactive PLC Learning Media Based on Virtual Learning

This research aims to develop interactive PLC learning media based on virtual learning. The interactive PLC learning media uses a 3D simulator design to simulate the application of PLC in the industry, so it looks like real and makes students more interested in learning PLC This study uses experimental methods. The 3D simulation software is Factory I/O developed by Real Games. It enables us to build a conveyor virtual plant and to bring it into the classroom. The automatic controller used to control the virtual plant is the PLC Siemens S7-1200 with Tia Portal software. The PLC is connected to the computer server through an ethernet network via TCP/IP protocol. With the rapid development of current internet technology, PLC learning can be done in virtual learning. This PLC learning media can be run remotely through the internet with team viewer program so that PLC learning is not only done on campus but can be done anywhere. From the results of research that has been done, the user can create PLC programs remotely to control conveyor virtual plant on a computer server through an internet network without having to install the program on their computers.&#x0D; Keywords: PLC, interactive learning media, virtual learning, remote desktop