Victoria Blue Staining Research Articles

Hepatitis B surface antigen in hepatocellular carcinoma.

Hepatitis B surface antigen (HBs-Ag) was detected histologically (by victoria blue-nuclear fast red staining) in the liver in 20-40% of liver cirrhosis and/or hepatocellular carcinoma (hepatoma) cases. In hepatoma cases, HBs-Ag was usually found in non-cancerous areas of the liver. However, some HBs-Ag positive cells were also found dispersed in cancerous areas; these were regarded as HBs-Ag-infected non-cancerous hepatic cells remaining undestroyed. There were a few cases where inconsistency was found between the results of the immunofluorescence technique using anti HBs-Ag serum and victoria blue staining in detecting HBs-Ag. This phenomenon was rare in non-cancerous areas, but was relatively frequent in cancerous areas.

The preepiglottic and paraglottic spaces in relation to spread of carcinoma of the larynx

The submucosal compartments of the larynx were examined using a whole-organ sectioning technique. Seven specimens of normal larynx and 35 specimens of carcinoma of the larynx were studied. Victoria blue and van Gieson stains were used to demonstrate connective tissue elements. The preepiglottic space was distinctly separated from the paraglottic spaces by membranous partitions made up of collagen and elastic fibers. These partitions offer some resistance to tumor invasion in the early stages of laryngeal cancer, keeping the tumor from extending to the adjacent compartment while allowing it to spread freely within the same compartment. In the absence of mucosal extension across the midline, submucosal spread of tumor to the opposite side is unlikely. In view of possible cranial spread of tumor along the preepiglottic and paraglottic spaces, excision of all these spaces, together with the hyoid bone, is indicated in total laryngectomy.

The structure of 180° supernumerary limbs and a hypothesis of their formation

The results of a detailed analysis of 100 supernumerary limbs generated by 180° ipsilateral rotation (on the same limb stump) of regeneration blastemas is presented. The limbs were analyzed in terms of their position of origin, frequency, cartilage structure by Victoria blue staining, and muscle structure by serial sections. Single, double, or triple supernumeraries can be produced at no unique position of origin, although the posterodorsal quadrant was preferred. Four classes of supernumerary limbs were generated by such operations—normal; double dorsal or double ventral; part normal/part mirror imaged; part normal/part inverted in approximately equal frequencies. After amputation of these supernumeraries the same muscle patterns are faithfully regenerated. A hypothesis to explain the production of these abnormal limbs is proposed based on the observed phenomenon of fusion of supernumerary blastemata, but their regenerative behaviour presents problems for current models of pattern formation. Similar results have been obtained with developing limb buds and the relation between development and regeneration is discussed.

A simple manoeuver to prevent loss of sections for performic acid–Victoria blue technique and a comparison of various neurosecretory stains

It is shown that whole brains of migratory locusts can be oxidized with performic acid and that paraffin sections of these can be successfully stained with Victoria blue. Either performic acid or acid permanganate oxidants can be used for Victoria blue and aldehyde fuchsin staining techniques thus allowing crisscross comparisons.

Adaptation of certain histological techniques for in situ demonstration of the neuro-endocrine system of insects and other animals

ABSTRACT Three techniques for staining the secretory neurones in sections were applied directly to the whole brain and/or intact organs of the neuro-endocrine system of certain insects, and the whole brain of various invertebrates and vertebrates. After minor changes in the original procedures, in situ staining was achieved in those components of the neuro-endocrine system that are known to contain the neurosecretory material. With the Victoria blue staining technique, the secretory neurones, the neurosecretory pathway, and the storage-and-release organ were stained satisfactorily in all the experimental animals, in such a way that observations could be made in whole mounts or suitably dissected portions of the bulk-stained preparations. With the aldehyde-fuchsin and aldehyde-thionin staining techniques, the somata and the proximal portion of the axon of the neurones and the storage-and-release organ were usually stained satisfactorily enough for purposes of observation in the invertebrate material only. On sectioning the bulk-stained components of the neuroendocrine system and mounting the sections, the sites known to contain the neurosecretory material were revealed promptly. On comparing the information derived from mounts of the bulk-stained preparations with that derived from sections of similar preparations, and also with that derived from routine histological procedure, no difference was detected.

LIGHT MICROSCOPICAL STUDIES OF ACRYLIC FIBERS

The present paper deals with three different kinds of light microscopical techniques which can be used successfully for the purpose of investigating acrylic fibers:(1) Phase contrast microscope.(2) Basic dye (Victoria Blue) staining.(3) Sandocryl (Sandocryl Black) staining.Several results obtained from a variety of commercial acrylic fibers are presented for illustration. See the figures in the text. In every figure three sheets of photomicrograph are arranged in the same order from left to right: (1) phase contrast picture, (2) Victoria Blue staining, and (3) Sandocryl Black staining. The magnification is fixed to. ca. 500 diameters throughout all figures.The phase contrast microscopy might be potentially used in combination with an electron microscopical study on ultrathin sections.The basic dye staining procedure is not only effective in revealing structural details of acrylic fibers under observation, but also will be of importamce in respect to providing informations about practical dyeability.Sandocryl staining should be remarked on its specificity against acrylic fibers, where copper (I) complex salt formation is involved. The two-component structure of Orlon 21 is demonstrated in a clearest manner.