Teaching science requires specific competencies that trained teachers in their previous education level. Teachers' educational backgrounds influence the implementation of science learning. Besides, the teaching experience is also an essential factor in determining the quality of science learning. This study aimed to identify how science teachers' educational background influences their way of teaching science. The data was obtained by observing three science teachers from Biology Education, Physics Education, and Biology when teaching the topics of Vibration and Excretory systems. The video recordings were then analyzed by using qualitative analysis software. The result showed that teachers from Biology Education backgrounds taught the Vibration topic exceeded the science teacher who had Physics Education degrees. According to teaching period and professional development program records, the teacher who had been teaching for 35 years outperformed the other two teachers. Therefore, science teachers are recommended to increase their participation in teacher training to improve science teaching skills.