You have accessJournal of UrologyProstate Cancer: Basic Research (III)1 Apr 2013496 FIBROBLAST GROWTH FACTOR 9 IN PROSTATE CANCER CELLS IS ASSOCIATED WITH POSTOPERATIVE RECURRENCE THROUGH ACCELERATION OF MESENCHYMAL TRANSITION, PROLIFERATION, AND INVASION Jun Teishima, Koichi Shoji, Tetsutaro Hayashi, Shigeki Yano, Kiyotaka Oka, Hirotaka Nagamatsu, Shinya Ohara, and Akio Matsubara Jun TeishimaJun Teishima Hiroshima, Japan More articles by this author , Koichi ShojiKoichi Shoji Hiroshima, Japan More articles by this author , Tetsutaro HayashiTetsutaro Hayashi Hiroshima, Japan More articles by this author , Shigeki YanoShigeki Yano Hiroshima, Japan More articles by this author , Kiyotaka OkaKiyotaka Oka Hiroshima, Japan More articles by this author , Hirotaka NagamatsuHirotaka Nagamatsu Hiroshima, Japan More articles by this author , Shinya OharaShinya Ohara Hiroshima, Japan More articles by this author , and Akio MatsubaraAkio Matsubara Hiroshima, Japan More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2013.02.1890AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Disruption of the fibroblast growth factor (FGF) signaling pathway in the prostate has been reported to cause failure of tissue homeostasis and development of malignant disease. Among the FGF family, FGF9 enhances cell proliferation and invasiveness in several malignant diseases. The aim of the present study is to investigate the role of FGF9 in prostate cancer cells. METHODS Cell viability and invasion of LNCaP and PC3 cells were assessed by using MTT assay and Matrigel invasion assay, respectively, by treatment with recombinant FGF9. Expression of MMPs, VEGFs, and cadherins in LNCaP by incubation with FGF9 was assessed by western blot analysis. Tissues obtained during a radical prostatectomy in 133 male patients were immunohistochemically stained by using anti-FGF9, anti-cadherin, anti-VEGF, and anti-MMP antibodies. RESULTS Cell viability and invasion of LNCaP and PC3 cells were significantly enhanced by treatment with recombinant FGF9. These effects were significantly suppressed by treatment with anti-FGF9 neutralizing antibody. Expression of N-cadherin, VEGF-A, and MMP2 were induced in LNCaP cells incubated in medium with FGF9 for five days. Immunohistochemical staining detected FGF9-positive cells in 20 samples. Furthermore, the immunoreactivity of N-cadherin and VEGF-A was highly detected in FGF9-positive samples. The prevalence of FGF9-positive cells was 34.2% in cases diagnosed with a Gleason score of 8 or higher (vs. Gleason score of 7 or lower, 7.4%, p=0.0003), 43.8% with seminal vesicle invasion (vs. without seminal vesicle invasion, 11.1%, p=0.0022), or 27.7% for those whose serum PSA level was higher than 10 ng/ml (vs. those whose serum PSA level was 10 ng/ml or lower, 8.1%, p=0.0058), significantly higher in comparison with other cases. The third-year biochemical relapse-free survival rate was 17.5% in cases with FGF9-positive cells, which was significantly lower than that in cases in which FGF9-positive cells were not detectable (75.5%, p<0.0001). Multivariate analysis demonstrated that the presence of FGF9-positive cells was an independent predictive factor for biochemical recurrence after radical prostatectomy. CONCLUSIONS These data indicate that FGF9 can correlate with mesenchymal transition in prostate cancer cells. FGF9 can also stimulate proliferation and invasion in prostate cancer cells by inducing the expression of VEGF-A and MMP2, and thus, FGF9 could be a novel candidate as a factor predicting recurrence after radical prostatectomy. © 2013 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 189Issue 4SApril 2013Page: e203-e204 Advertisement Copyright & Permissions© 2013 by American Urological Association Education and Research, Inc.MetricsAuthor Information Jun Teishima Hiroshima, Japan More articles by this author Koichi Shoji Hiroshima, Japan More articles by this author Tetsutaro Hayashi Hiroshima, Japan More articles by this author Shigeki Yano Hiroshima, Japan More articles by this author Kiyotaka Oka Hiroshima, Japan More articles by this author Hirotaka Nagamatsu Hiroshima, Japan More articles by this author Shinya Ohara Hiroshima, Japan More articles by this author Akio Matsubara Hiroshima, Japan More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...